alpha Actinin (ACTN1) (NM_001102) Human Untagged Clone

CAT#: SC119433

ACTN1 (untagged)-Human actinin, alpha 1 (ACTN1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: alpha Actinin
• Synonyms: BDPLT15
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119433 sequence for NM_001102 edited (data generated by NextGen Sequencing)
  ATGGACCATTATGATTCTCAGCAAACCAACGATTACATGCAGCCAGAAGAGGACTGGGAC
  CGGGACCTGCTCCTGGACCCGGCCTGGGAGAAGCAGCAGAGAAAGACATTCACGGCATGG
  TGTAACTCCCACCTCCGGAAGGCGGGGACACAGATCGAGAACATCGAAGAGGACTTCCGG
  GATGGCCTGAAGCTCATGCTGCTGCTGGAGGTCATCTCAGGTGAACGCTTGGCCAAGCCA
  GAGCGAGGCAAGATGAGAGTGCACAAGATCTCCAACGTCAACAAGGCCCTGGATTTCATA
  GCCAGCAAAGGCGTCAAACTGGTGTCCATCGGAGCCGAAGAAATCGTGGATGGGAATGTG
  AAGATGACCCTGGGCATGATCTGGACCATCATCCTGCGCTTTGCCATCCAGGACATCTCC
  GTGGAAGAGACTTCAGCCAAGGAAGGGCTGCTCCTGTGGTGTCAGAGAAAGACAGCCCCT
  TACAAAAATGTCAACATCCAGAACTTCCACATAAGCTGGAAGGATGGCCTCGGCTTCTGT
  GCTTTGATCCACCGACACCGGCCCGAGCTGATTGACTACGGGAAGCTGCGGAAGGATGAT
  CCACTCACAAATCTGAATACGGCTTTTGACGTGGCAGAGAAGTACCTGGACATCCCCAAG
  ATGCTGGATGCCGAAGACATCGTTGGAACTGCCCGACCGGATGAGAAAGCCATCATGACT
  TACGTGTCTAGCTTCTACCACGCCTTCTCTGGAGCCCAGAAGGCGGAGACAGCAGCCAAT
  CGCATCTGCAAGGTGTTGGCCGTCAACCAGGAGAACGAGCAGCTTATGGAAGACTACGAG
  AAGCTGGCCAGTGATCTGTTGGAGTGGATCCGCCGCACAATCCCGTGGCTGGAGAACCGG
  GTGCCCGAGAACACCATGCATGCCATGCAACAGAAGCTGGAGGACTTCCGGGACTACCGG
  CGCCTGCACAAGCCGCCCAAGGTGCAGGAGAAGTGCCAGCTGGAGATCAACTTCAACACG
  CTGCAGACCAAGCTGCGGCTCAGCAACCGGCCTGCCTTCATGCCCTCTGAGGGCAGGATG
  GTCTCGGACATCAACAATGCCTGGGGCTGCCTGGAGCAGGTGGAGAAGGGCTATGAGGAG
  TGGTTGCTGAATGAGATCCGGAGGCTGGAGCGACTGGACCACCTGGCAGAGAAGTTCCGG
  CAGAAGGCCTCCATCCACGAGGCCTGGACTGACGGCAAAGAGGCCATGCTGCGACAGAAG
  GACTATGAGACCGCCACCCTCTCGGAGATCAAGGCCTTGCTCAAGAAGCATGAGGCCTTC
  GAGAGTGACCTGGCTGCCCACCAGGACCGTGTGGAGCAGATTGCCGCCATCGCACAGGAG
  CTCAATGAGCTGGACTATTATGACTCACCCAGTGTCAACGCCCGTTGCCAAAAGATCTGT
  GACCAGTGGGACAATCTGGGGGCCCTAACTCAGAAGCGAAGGGAAGCTCTGGAGCGGACC
  GAGAAACTGCTGGAGACCATTGACCAGCTGTACTTGGAGTATGCCAAGCGGGCTGCACCC
  TTCAACAACTGGATGGAGGGGGCCATGGAGGACCTGCAGGACACCTTCATTGTGCACACC
  ATTGAGGAGATCCAGGGACTGACCACAGCCCATGAGCAGTTCAAGGCCACCCTCCCTGAT
  GCCGACAAGGAGCGCCTGGCCATCCTGGGCATCCACAATGAGGTGTCCAAGATTGTCCAG
  ACCTACCACGTCAATATGGCGGGCACCAACCCCTACACAACCATCACGCCTCAGGAGATC
  AATGGCAAATGGGACCACGTGCGGCAGCTGGTGCCTCGGAGGGACCAAGCTCTGACGGAG
  GAGCATGCCCGACAGCAGCACAATGAGAGGCTACGCAAGCAGTTTGGAGCCCAGGCCAAT
  GTCATCGGGCCCTGGATCCAGACCAAGATGGAGGAGATCGGGAGGATCTCCATTGAGATG
  CATGGGACCCTGGAGGACCAGCTCAGCCACCTGCGGCAGTATGAGAAGAGCATCGTCAAC
  TACAAGCCAAAGATTGATCAGCTGGAGGGCGACCACCAGCTCATCCAGGAGGCGCTCATC
  TTCGACAACAAGCACACCAACTACACCATGGAGCACATCCGTGTGGGCTGGGAGCAGCTG
  CTCACCACCATCGCCAGGACCATCAATGAGGTAGAGAACCAGATCCTGACCCGGGATGCC
  AAGGGCATCAGCCAGGAGCAGATGAATGAGTTCCGGGCCTCCTTCAACCACTTTGACCGG
  GATCACTCCGGCACACTGGGTCCCGAGGAGTTCAAAGCCTGCCTCATCAGCTTGGGTTAT
  GATATTGGCAACGACCCCCAGGGAGAAGCAGAATTTGCCCGCATCATGAGCATTGTGGAC
  CCCAACCGCCTGGGGGTAGTGACATTCCAGGCCTTCATTGACTTCATGTCCCGCGAGACA
  GCCGACACAGATACAGCAGACCAAGTCATGGCTTCCTTCAAGATCCTGGCTGGGGACAAG
  AACTACATTACCATGGACGAGCTGCGCCGCGAGCTGCCACCCGACCAGGCTGAGTACTGC
  ATCGCGCGGATGGCCCCCTACACCGGCCCCGACTCCGTGCCAGGTGCTCTGGACTACATG
  TCCTTCTCCACGGCGCTGTACGGCGAGAGTGACCTCTAA
  
  Clone variation with respect to NM_001102.3
  1297 c=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001102 unedited
  NGGTTTAAATTTGTATACGACTCACTATAGGGCGGCCGCGAATTCGCACGAGGCTAGTTC
  AGTGTGCGTGGAGATTAGGTCCAAGCGCCCGCCCAGAGGCGGCAGTCCGCGAGCCCAGCC
  GCCGCTGTCGCCGCCAGTAGCAGCCTTCGCCAGCAGCGCCGCGGCGGAACCGGGCGCAGG
  GGAGCGAGCCCGGCCCCGCCAGCCCAGCCCAGCCCAGCCCAGCCCTACTCCCTCCCCACG
  CCAGGGCAGCAGCCGTTGCTCAGAGAGAAGGTGGAGGAAGAAATCCAGACCCTAGCACGC
  GCGCACCATCATGGACCATTATGATTCTCAGCAAACCAACGATTACATGCAGCCAGAAGA
  GGACTGGGACCGGGACCTGCTCCTGGACCCGGCCTGGGAGAAGCAGCAGAGAAAGACATT
  CACGGCATGGTGTAACTCCCACCTCCGGAAGGCGGGGACACAGATCGAGAACATCGAAGA
  GGACTTCCGGGATGGCCTGAAGCTCATGCTGCTGCTGGAGGTCATCTCAGGTGAACGCTT
  GGCCAAGCCAGAGCGAGGCAAGATGAGAGTGCACAAGATCTCCAACGTCAACAAGGCCCT
  GGATTTCATAGCCAGCAAAGGCGTCAAACTGGTGTCCATCGGAGCCCGAAGAAATCGTGG
  ATTGGGGAATGTGAAGATGACCCTGTGCATGACCTGGCCATCATCCTGCGCTTTGGCATC
  CAGGACATCTCCGTGGAAGAGACTTCAGCCAAGGAAGGCCTGCTCCTGTGGTGTCAGAGC
  AAGACAGCCCCTTACAAAATGTAAACCTTCAGAACTCTCACATAGCTGGAAGGATGGCCT
  CGCTTCTGTGCTTTGATCCACCGACACCGGCCCCACCTGTATTGACTCGGTAAGCTGCGG
  AAG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001102 unedited
  CGCGGCACGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGTCTAGGAGTGTGGTTTT
  TTTAATACCATCTGTGCCAAGCAATATCAAAGTTGTCCATACTCTCCAAGTTTGTCCTAT
  TTTTAATAGACAATATTAAAAATTTTTTAAGTGTGTGCTGATAAAGAGACTAGTCACACC
  TCTGAGGGGATTTAAAAATGAGAAGCCACATAAATTCTCTTCCTTAAAAAACAGCCATTT
  CAAAAGATTTTCTTTAATATCATAAAATATTTTCATGGACAAGTGAGCTAGCAAACACAC
  ATGCACCAATGTGCCTTTTGACAAGAGTACCCCCTACCCCGACTCCCACACCAAAATGGA
  CATGAGATTGGAGAAATGAATACAGCAGATGGAACAGATAGAAGAAAAAAAATCAGTAAA
  AAGGATGGAATATAACTTTGTGCTTGGTTATTTTCCTATGTCGCAACAAAACAAGACATT
  TCGGTGCAAATAGTTAATCTTTCCTCTTTTTTTCCATTTGTCTGGTGGAGAAAAAATACT
  TTTTCTATAATAAAATATGTAGTTTTTTGGTTTTTAACGTAACTTTTTTTTTCTTTTTTG
  CAGAAAATAATTTTGTAAACTGTCACTTCGCGGGCAGGGAGGATCGATGCCACGTGGGCC
  CCAGCTCACCCGGGTGGAGGCTGGGAGCTGAAACCGAACCCAGCAGGATATGGGCGACGG
  CGGATGTGCAAGGCATGGCACGGCGCACAATACNAGGGCGGCCGGGCGGGGTGGATTAAA
  GGTCACTCTCGCCGTACAGCGCCGTGGAGATGACATGTANTCCATAACACCCTGCACGGA
  GTCGGGGCCGTGTTAGGGGCCCATCCCGCGCAGCAGTACTCANCCTGTTTCGGTGGCACC
  TCGCGGCGCAACTCGNCCATGGTAATGTAANTCCTTGCCCCCAGCAAGATCTTGTAGGNA
  AGCATGACTGTGGCTGCTGTN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001102
• Insert Size: 3520 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001102.2, NP_001093.1
• RefSeq Size: 3398 bp
• RefSeq ORF: 2679 bp
• Locus ID: 87
• UniProt ID: P12814
• Cytogenetics: 14q22-q24
• Domains: CH, spectrin, EFh
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS
• Protein Pathways: Adherens junction, Arrhythmogenic right ventricular cardiomyopathy (ARVC), Focal adhesion, Leukocyte transendothelial migration, Regulation of actin cytoskeleton, Systemic lupus erythematosus, Tight junction
• Gene Summary: Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, cytoskeletal, alpha actinin isoform and maps to the same site as the structurally similar erythroid beta spectrin gene. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (b) has the same N- and C-termini but is shorter compared to isoform a.