Angiopoietin 2 (ANGPT2) (NM_001147) Human Untagged Clone

CAT#: SC119420

ANGPT2 (untagged)-Human angiopoietin 2 (ANGPT2), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Angiopoietin 2
• Synonyms: AGPT2; ANG2; LMPHM10
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119420 sequence for NM_001147 edited (data generated by NextGen Sequencing)
  ATGTGGCAGATTGTTTTCTTTACTCTGAGCTGTGATCTTGTCTTGGCCGCAGCCTATAAC
  AACTTTCGGAAGAGCATGGACAGCATAGGAAAGAAGCAATATCAGGTCCAGCATGGGTCC
  TGCAGCTACACTTTCCTCCTGCCAGAGATGGACAACTGCCGCTCTTCCTCCAGCCCCTAC
  GTGTCCAATGCTGTGCAGAGGGACGCGCCGCTCGAATACGATGACTCGGTGCAGAGGCTG
  CAAGTGCTGGAGAACATCATGGAAAACAACACTCAGTGGCTAATGAAGCTTGAGAATTAT
  ATCCAGGACAACATGAAGAAAGAAATGGTAGAGATACAGCAGAATGCAGTACAGAACCAG
  ACGGCTGTGATGATAGAAATAGGGACAAACCTGTTGAACCAAACAGCTGAGCAAACGCGG
  AAGTTAACTGATGTGGAAGCCCAAGTATTAAATCAGACCACGAGACTTGAACTTCAGCTC
  TTGGAACACTCCCTCTCGACAAACAAATTGGAAAAACAGATTTTGGACCAGACCAGTGAA
  ATAAACAAATTGCAAGATAAGAACAGTTTCCTAGAAAAGAAGGTGCTAGCTATGGAAGAC
  AAGCACATCATCCAACTACAGTCAATAAAAGAAGAGAAAGATCAGCTACAGGTGTTAGTA
  TCCAAGCAAAATTCCATCATTGAAGAACTAGAAAAAAAAATAGTGACTGCCACGGTGAAT
  AATTCAGTTCTTCAAAAGCAGCAACATGATCTCATGGAGACAGTTAATAACTTACTGACT
  ATGATGTCCACATCAAACTCAGCTAAGGACCCCACTGTTGCTAAAGAAGAACAAATCAGC
  TTCAGAGACTGTGCTGAAGTATTCAAATCAGGACACACCACAAATGGCATCTACACGTTA
  ACATTCCCTAATTCTACAGAAGAGATCAAGGTCTACTGTGACATGGAAGCTGGAGGAGGC
  GGGTGGACAATTATTCAGCGACGTGAGGATGGCAGCGTTGATTTTCAGAGGACTTGGAAA
  GAATATAAAGTGGGATTTGGTAACCCTTCAGGAGAATATTGGCTGGGAAATGAGTTTGTT
  TCGCAACTGACTAATCAGCAACGCTATGTGCTTAAAATACACCTTAAAGACTGGGAAGGG
  AATGAGGCTTACTCATTGTATGAACATTTCTATCTCTCAAGTGAAGAACTCAATTATAGG
  ATTCACCTTAAAGGACTTACAGGGACAGCCGGCAAAATAAGCAGCATCAGCCAACCAGGA
  AATGATTTTAGCACAAAGGATGGAGACAACGACAAATGTATTTGCAAATGTTCACAAATG
  CTAACAGGAGGCTGGTGGTTTGATGCATGTGGTCCTTCCAACTTGAACGGAATGTACTAT
  CCACAGAGGCAGAACACAAATAAGTTCAACGGCATTAAATGGTACTACTGGAAAGGCTCA
  GGCTATTCGCTCAAGGCCACAACCATGATGATCCGACCAGCAGATTTCTAA
  
  Clone variation with respect to NM_001147.2
  408 g=>t;735 g=>a;882 g=>a;932 c=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001147 unedited
  AAAGTCAGAATTGTATACGACTCATATAGGCGGCCGCGCAATTCGCACGAGGCCTCGTGC
  CGAATTCNGCACGAGGCATGGGGCCTGGGTGGGGGGCAGGCATTCTGCTCTGATTTTTCC
  TGTGGCCTGGCTAGTGACCCCCTACAGGGAAGATAACGGCTAAGCCAGGAGGGCGGAGCA
  GCCCACTACACATGTCTGGCTGCTCTTATCAACTTATCATATAANGAAAGGAAAGTGATT
  GAGTCGGATACTGACACTGTAGGATCTGGGGAGAGAGGAACAAAGGACCGTGAAAGCTGC
  TCTGTAAAAGCTGACACAGCCCTCCCAAGTGAGCAGGAGTGGTCTTCCCACTGGAATCTG
  ACAGTTTACTGCATGCCTGGAGAGAACACAGCAGTAAAAACCAGGTTTGCTACTGGAAAA
  AGAGGAAAGAGAAGACTTTCATTGGCGGACCCAGCCATGGCAGCGTAGCAGCCCTGCGTT
  TCAGACGGCAGCAGCTCGGGACTCTGGACGTGTGTTTGCCCTCAAGTTTGCTAAGCTGCT
  GGTTTATTACTGAAGAAAGAATGTGGCAGATTGGTTTCTTTACTCTGAGCTGTGATCTTG
  GCTTGGCCGCAGCCTATAACAACTTTCGGAAGAGCATGGACAGCATANGAAAGAAGCAAT
  ATCAGGTGCAGCATGGGTCCTGCAGCTACACTTTCCTCCTGCGAGAGATGGACAACTGCC
  GCTCTTCCTCCAGCCCCTACGTGTCCAATGCTGTGCANAGGGACCCGCCGCTCNAATACA
  ATGACTCCGTGCANAGGCTGCAAGTGCTGGAGAACATCATGGGAAACACACTCAGTGGCT
  AATGAAGCTTGAGAATATATCCGGGACACATGAAGAAAGAATGGTGGAATACAGCAGATG
  CGGTCAA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001147 unedited
  GCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTATCCCCTAATAAGTTATATTTAA
  TTACTAAAAATACCTTCATATTTAACAATCAGGCAGAAAAAAATAGTACGGTCTGCATAT
  AAACTAAAATGGCACGTTTCTGTTGATAATTTCAGAGATTCTGGAAGTTTCTACCATATA
  AATTTGAAATACGTATTTGAGCATTAACTTATAACTAAGCTGTCAACATAAATGTAAATA
  CGCTGTTTTTGAAATAAAAATTTAAAGCACCTAAGAGATGGAGTAAAAATGCACTAACTG
  TTTTTCCAAATATTAAACTTCTAGTAACCCCTTCTCAGAATATCCCTGAATATGTCTTTT
  TATGGCTTAGAGAGTTTTTTTCTTCCTTTTAATTGTGATAGTGATGGTGAATTCAGGACA
  TATGGGTATTTACACAGTGTATAAACAGTGCTCAGAAGAATGCAGTTCCAAGATGATCTG
  TATTGTATAACATAAGTGTTCTGTTTTCCAGTTATTTACTGATAAACTTGCACATAACAT
  TCTTGGTTGTGACAGCAGCGTCTGTAAACTGTCAGTCTGATTCTCAGCCTCGGGTTCATC
  TTTGCATAGGTGTTCTGTCTAATCACAATTATGGATGTTTAGGGTCTTGCTTTGGTCCGT
  TAAGTGATGCAAGTTTAAGTGATAAAGTTTACAGGCTCTAATCTGGAGCATGTGGGTCCC
  GTCAGCACCGAGCACACGCCCTCTGTGGGTGGAAGAGACACAGTGCGCAGCCGTGACTTT
  CAGTGCACTGGGCTTAAGTCTTTGAAATAGTTCGAGACAGTCCTTCAGTGACCTGGATGT
  TTAGAATCTGCTGGTCGGACATCATGGNTGTGGCCTTGAGCGATAGCCTGACCCTTTCAG
  TATACCATNTAATGCCGNTGACTTATTTGGGGTCTGGCCCTGGGGAAAGNACATTCCGCT
  CAAGNTGGAGGGACACATGCATCAACCACCACCTNCT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001147
• Insert Size: 2770 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001147.1, NP_001138.1
• RefSeq Size: 2269 bp
• RefSeq ORF: 1491 bp
• Locus ID: 285
• UniProt ID: O15123
• Cytogenetics: 8p23.1
• Domains: FBG
• Protein Families: Druggable Genome, Secreted Protein
• Gene Summary: This gene belongs to the angiopoietin family of growth factors. The protein encoded by this gene is an antagonist of angiopoietin 1, and both angiopoietin 1 and angiopoietin 2 are ligands for the endothelial TEK receptor tyrosine kinase. Angiopoietin 2 is upregulated in multiple inflammatory diseases and is implicated in the direct control of inflammation-related signaling pathways. The encoded protein affects angiogenesis during embryogenesis and tumorigenesis, disrupts the vascular remodeling ability of angiopoietin 1, and may induce endothelial cell apoptosis. This gene serves a prognostic biomarker for acute respiratory distress syndrome. [provided by RefSeq, Aug 2020]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments.