BubR1 (BUB1B) (NM_001211) Human Untagged Clone

CAT#: SC119395

BUB1B (untagged)-Human budding uninhibited by benzimidazoles 1 homolog beta (yeast) (BUB1B)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: BubR1
• Synonyms: Bub1A; BUB1beta; BUBR1; hBUBR1; MAD3L; MVA1; SSK1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_001211 edited
  ATGGCGGCGGTGAAGAAGGAAGGGGGTGCTCTGAGTGAAGCCATGTCCCTGGAGGGAGAT
  GAATGGGAACTGAGTAAAGAAAATGTACAACCTTTAAGGCAAGGGCGGATCATGTCCACG
  CTTCAGGGAGCACTGGCACAAGAATCTGCCTGTAACAATACTCTTCAGCAGCAGAAACGG
  GCATTTGAATATGAAATTCGATTTTACACTGGAAATGACCCTCTGGATGTTTGGGATAGG
  TATATCAGCTGGACAGAGCAGAACTATCCTCAAGGTGGGAAGGAGAGTAATATGTCAACG
  TTATTAGAAAGAGCTGTAGAAGCACTACAAGGAGAAAAACGATATTATAGTGATCCTCGA
  TTTCTCAATCTCTGGCTTAAATTAGGGCGTTTATGCAATGAGCCTTTGGATATGTACAGT
  TACTTGCACAACCAAGGGATTGGTGTTTCACTTGCTCAGTTCTATATCTCATGGGCAGAA
  GAATATGAAGCTAGAGAAAACTTTAGGAAAGCAGATGCGATATTTCAGGAAGGGATTCAA
  CAGAAGGCTGAACCACTAGAAAGACTACAGTCCCAGCACCGACAATTCCAAGCTCGAGTG
  TCTCGGCAAACTCTGTTGGCACTTGAGAAAGAAGAAGAGGAGGAAGTTTTTGAGTCTTCT
  GTACCACAACGAAGCACACTAGCTGAACTAAAGAGCAAAGGGAAAAAGACAGCAAGAGCT
  CCAATCATCCGTGTAGGAGGTGCTCTCAAGGCTCCAAGCCAGAACAGAGGACTCCAAAAT
  CCATTTCCTCAACAGATGCAAAATAATAGTAGAATTACTGTTTTTGATGAAAATGCTGAT
  GAGGCTTCTACAGCAGAGTTGTCTAAGCCTACAGTCCAGCCATGGATAGCACCCCCCATG
  CCCAGGGCCAAAGAGAATGAGCTGCAAGCAGGCCCTTGGAACACAGGCAGGTCCTTGGAA
  CACAGGCCTCGTGGCAATACAGCTTCACTGATAGCTGTACCCGCTGTGCTTCCCAGTTTC
  ACTCCATATGTGGAAGAGACTGCACGACAGCCAGTTATGACACCATGTAAAATTGAACCT
  AGTATAAACCACATCCTAAGCACCAGAAAGCCTGGAAAGGAAGAAGGAGATCCTCTACAA
  AGGGTTCAGAGCCATCAGCAAGCGTCTGAGGAGAAGAAAGAGAAGATGATGTATTGTAAG
  GAGAAGATTTATGCAGGAGTAGGGGAATTCTCCTTTGAAGAAATTCGGGCTGAAGTTTTC
  CGGAAGAAATTAAAAGAGCAAAGGGAAGCCGAGCTATTGACCAGTGCAGAGAAGAGAGCA
  GAAATGCAGAAACAGATTGAAGAGATGGAGAAGAAGCTAAAAGAAATCCAAACTACTCAG
  CAAGAAAGAACAGGTGATCAGCAAGAAGAGACGATGCCTACAAAGGAGACAACTAAACTG
  CAAATTGCTTCCGAGTCTCAGAAAATACCAGGAATGACTCTATCCAGTTCTGTTTGTCAA
  GTAAACTGTTGTGCCAGAGAAACTTCACTTGCGGAGAACATTTGGCAGGAACAACCTCAT
  TCTAAAGGTCCCAGTGTACCTTTCTCCATTTTTGATGAGTTTCTTCTTTCAGAAAAGAAG
  AATAAAAGTCCTCCTGCAGATCCCCCACGAGTTTTAGCTCAACGAAGACCCCTTGCAGTT
  CTCAAAACCTCAGAAAGCATCACCTCAAATGAAGATGTGTCTCCAGATGTTTGTGATGAA
  TTTACAGGAATTGAACCCTTGAGCGAGGATGCCATTATCACAGGCTTCAGAAATGTAACA
  ATTTGTCCTAACCCAGAAGACACTTGTGACTTTGCCAGAGCAGCTCGTTTTGTATCCACT
  CCTTTTCATGAGATAATGTCCTTGAAGGATCTCCCTTCTGATCCTGAGAGACTGTTACCG
  GAAGAAGATCTAGATGTAAAGACCTCTGAGGACCAGCAGACAGCTTGTGGCACTATCTAC
  AGTCAGACTCTCAGCATCAAGAAGCTGAGCCCAATTATTGAAGACAGTCGTGAAGCCACA
  CACTCCTCTGGCTTCTCTGGTTCTTCTGCCTCGGTTGCAAGCACCTCCTCCATCAAATGT
  CTTCAAATTCCTGAGAAACTAGAACTTACTAATGAGACTTCAGAAAACCCTACTCAGTCA
  CCATGGTGTTCACAGTATCGCAGACAGCTACTGAAGTCCCTACCAGAGTTAAGTGCCTCT
  GCAGAGTTGTGTATAGAAGACAGACCAATGCCTAAGTTGGAAATTGAGAAGGAAATTGAA
  TTAGGTAATGAGGATTACTGCATTAAACGAGAATACCTAATATGTGAAGATTACAAGTTA
  TTCTGGGTGGCGCCAAGAAACTCTGCAGAATTAACAGTAATAAAGGTATCTTCTCAACCT
  GTCCCATGGGACTTTTATATCAACCTCAAGTTAAAGGAACGTTTAAATGAAGATTTTGAT
  CATTTTTGCAGCTGTTATCAATATCAAGATGGCTGTATTGTTTGGCACCAATATATAAAC
  TGCTTCACCCTTCAGGATCTTCTCCAACACAGTGAATATATTACCCATGAAATAACAGTG
  TTGATTATTTATAACCTTTTGACAATAGTGGAGATGCTACACAAAGCAGAAATAGTCCAT
  GGTGACTTGAGTCCAAGGTGTCTGATTCTCAGAAACAGAATCCACGATCCCTATGATTGT
  AACAAGAACAATCAAGCTTTGAAGATAGTGGACTTTTCCTACAGTGTTGACCTTAGGGTG
  CAGCTGGATGTTTTTACCCTCAGCGGCTTTCGGACTGTACAGATCCTGGAAGGACAAAAG
  ATCCTGGCTAACTGTTCTTCTCCCTACCAGGTAGACCTGTTTGGTATAGCAGATTTAGCA
  CATTTACTATTGTTCAAGGAACACCTACAGGTCTTCTGGGATGGGTCCTTCTGGAAACTT
  AGCCAAAATATTTCTGAGCTAAAAGATGGTGAATTGTGGAATAAATTCTTTGTGCGGATT
  CTGAATGCCAATGATGAGGCCACAGTGTCTGTTCTTGGGGAGCTTGCAGCAGAAATGAAT
  GGGGTTTTTGACACTACATTCCAAAGTCACCTGAACAAAGCCTTATGGAAGGTAGGGAAG
  TTAACTAGTCCTGGGGCTTTGCTCTTTCAGTGA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001211 unedited
  NGTTCAAATTTGTATACGACTCCTATAGGGCGGCCGCGAATTCGCACGAGGGGTTTGTTA
  GGGAGTCGTGTACGTGCCTTGGTCGCTTCTGTAGCTCCGAGGGCAGGTTGCGGAAGAAAG
  CCCAGGCGGTCTGTGGCCCAGAGGAAAGGCCTGCAGCAGGACGAGGACCTGAGCCAGGAA
  TGCAGGATGGCGGCGGTGAAGAAGGAAGGGGGTGCTCTGAGTGAAGCCATGTCCCTGGAG
  GGAGATGAATGGGAACTGAGTAAAGAAAATGTACAACCTTTAAGGCAAGGGCGGATCATG
  TCCACGCTTCAGGGAGCACTGGCACAAGAATCTGCCTGTAACAATACTCTTCAGCAGCAG
  AAACGGGCATTTGAATATGAAATTCGATTTTACACTGGAAATGACCCTCTGGATGTTTGG
  GATAGGTATATCAGCTGGACAGAGCAGAACTATCCTCAAGGTGGGAAGGAGAGTAATATG
  TCAACGTTATTAGAAAGAGCTGTAGAAGCACTACAAGGAGAAAAACGATATTATAGTGAT
  CCTCGATTTCTCAATCTCTGGCTTAAATTAGGGCGTTTATGCAATGAGCCTTTGGATATG
  TACAGTTACTTGCACAACNCAAGGGATTGGTGTTTCACTTGCTCAGTTCTATATCTCATG
  GGCAGAAGAATATGAAGCTAGAGAAAACTTTAGGAAAGCAGATGCGATATTTCAGGAAGG
  GATTCAACAGAAGGCTGAACCACTAGAAAGACTACAGTCCCAGCACCGACAATTCCAAGC
  TCGAGTGTCTCGGCANACTCTGTTGGCACTTGAGAAAGAAGAGAGGNAGGNAAGTNNTTG
  AGTCTTCTGTACCACACGAAGCACACTAGNNCTGACTAAAGAGCAAGGGAANAAGACAGC
  AGAGCCCAATCATTCGGTTNAGGAGGTGCTCTCA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001211 unedited
  AATCCACTTTACAGGTGATCATAGAGACATTTACAATTACAAATGGGTTAAGTCAGGGAA
  AAGTAGAGACAAATGGGTTAAATAACAAGGTAACCATTTGTAATGAGTCTGTTTAGAATA
  AAATAGTTCTTCACAAAAGTTAGACAAGGCCATGAGTAAGTATATCACTGTATAAAAAAT
  ATCAGTGACGTCAAAATATACCTGTACCAAAAAGTAGAACAGCAATGGTAGTGCATCTAA
  ATGTGTCCTAAATTAAATTACAGCACATACAGTTTCAGTGTTCCACAATACAACCATTGC
  TCTGAGGCAGCAATCTGTGAGACTTGATCGCCTAGTTCACTGAAAGAGCAAAGCCCCAGG
  ACTAGTTAACTTCCCTACCTTCCATAATGCTTTGTTCCGGTGACTTTGGAATGTAGTGTC
  AAAAACCCCATTCATTTCTGCTGCAAGCTCCCCAAGAACAGACACTGCGGCCTCATCATT
  GGCATTTAGAATTCCGACAAAGATTTATTCCACATTCACCATCCTTTATCTCAGAATATC
  TCGGCTATGTTCCAGAAGACCCATCCCACAAACCTCGCCGGGTCCCTTGAACAATATCAA
  CGCGCTAAACCCGGTATTCCAAACCGCTCTCCTGCTAGGGAAAAAACGGTAGCCAGATCC
  TTTGCCCTTCCGGAATCTGCCAGTCCTATACCCGTTGAGGGTAAAACTCCCCGCTTGTCC
  CCACGGTTTTCCTCTGGCCGAATAGCCCCTCATTTTCAAGCTCGGTTGTTTTTGCCATAT
  TATAAGGTATCGGCTCTTCCCCTTGACAACATATTTTTGCACTCACCCACTATGGATCAC
  CTTTGCCTTGCGCCCCTTCCCTTCCCGTCATAGGTCCTACCCACACACCCGTCCTCTATT
  TTCCCCCTTCCCGCCCTGGTTATCTCCCACCGCCACGTTTCCTTTTTGTCGTTTATCCAC
  CACCCCGCCCCCGCTTTCCCACCCCCCACACCCCCTCACTTTCTCTCCCGCGCCTTTCTC
  TCCCG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001211
• Insert Size: 3900 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001211.4, NP_001202.4
• RefSeq Size: 3721 bp
• RefSeq ORF: 3153 bp
• Locus ID: 701
• UniProt ID: O60566
• Cytogenetics: 15q15.1
• Protein Families: Druggable Genome, Protein Kinase, Stem cell - Pluripotency
• Protein Pathways: Cell cycle
• Gene Summary: This gene encodes a kinase involved in spindle checkpoint function. The protein has been localized to the kinetochore and plays a role in the inhibition of the anaphase-promoting complex/cyclosome (APC/C), delaying the onset of anaphase and ensuring proper chromosome segregation. Impaired spindle checkpoint function has been found in many forms of cancer. [provided by RefSeq, Jul 2008]