Hsp47 (SERPINH1) (NM_001235) Human Untagged Clone

CAT#: SC119367

SERPINH1 (untagged)-Human serpin peptidase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1) (SERPINH1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Hsp47
• Synonyms: AsTP3; CBP1; CBP2; gp46; HSP47; OI10; PIG14; PPROM; RA-A47; SERPINH2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119367 sequence for NM_001235 edited (data generated by NextGen Sequencing)
  ATGCGCTCCCTCCTGCTTCTCAGCGCCTTCTGCCTCCTGGAGGCGGCCCTGGCCGCCGAG
  GTGAAGAAACCTGCAGCCGCAGCAGCTCCTGGCACTGCGGAGAAGTTGAGCCCCAAGGCG
  GCCACGCTTGCCGAGCGCAGCGCCGGCCTGGCCTTCAGCTTGTACCAGGCCATGGCCAAG
  GACCAGGCAGTGGAGAACATCCTGGTGTCACCCGTGGTGGTGGCCTCGTCGCTGGGGCTC
  GTGTCGCTGGGCGGCAAGGCGACCACGGCGTCGCAGGCCAAGGCAGTGCTGAGCGCCGAG
  CAGCTGCGCGACGAGGAGGTGCACGCCGGCCTGGGCGAGCTGCTGCGCTCACTCAGCAAC
  TCCACGGCGCGCAACGTGACCTGGAAGCTGGGCAGCCGACTGTACGGACCCAGCTCAGTG
  AGCTTCGCTGATGACTTCGTGCGCAGCAGCAAGCAGCACTACAACTGCGAGCACTCCAAG
  ATCAACTTCCGCGACAAGCGCAGCGCGCTGCAGTCCATCAACGAGTGGGCCGCGCAGACC
  ACCGACGGCAAGCTGCCCGAGGTCACCAAGGACGTGGAGCGCACGGACGGCGCCCTGTTA
  GTCAACGCCATGTTCTTCAAGCCACACTGGGATGAGAAATTCCACCACAAGATGGTGGAC
  AACCGTGGCTTCATGGTGACTCGGTCCTATACCGTGGGTGTCATGATGATGCACCGGACA
  GGCCTCTACAACTACTACGACGACGAGAAGGAAAAGCTGCAAATCGTGGAGATGCCCCTG
  GCCCACAAGCTCTCCAGCCTCATCATCCTCATGCCCCATCACGTGGAGCCTCTCGAGCGC
  CTTGAAAAGCTGCTAACCAAAGAGCAGCTGAAGATCTGGATGGGGAAGATGCAGAAGAAG
  GCTGTTGCCATCTCCTTGCCCAAGGGTGTGGTGGAGGTGACCCATGACCTGCAGAAACAC
  CTGGCTGGGCTGGGCCTGACTGAGGCCATTGACAAGAACAAGGCCGACTTGTCACGCATG
  TCAGGCAAGAAGGACCTGTACCTGGCCAGCGTGTTCCACGCCACCGCCTTTGAGTTGGAC
  ACAGATGGCAACCCCTTTGACCAGGACATCTACGGGCGCGAGGAGCTGCGCAGCCCCAAG
  CTGTTCTACGCCGACCACCCCTTCATCTTCCTAGTGCGGGACACCCAAAGCGGCTCCCTG
  CTATTCATTGGGCGCCTGGTCCGGCCTAAGGGTGACAAGATGCGAGACGAGTTATAG
  
  Clone variation with respect to NM_001235.3
  234 a=>g;598 c=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001235 unedited
  TTTGGATTTGTAATACGACTTTCCTATAGGCGGCCGACGAATTCGCACGAGGCGCGGCTC
  GAGAGCGAGAGTCACGTCCCGGCGCTAGCCCAGCCCGACCCATGCCCACCGTGGTGCACG
  CAAACCACTTCCTGGCCATGCGCTCCCTCCTGCTTCTCAGCGCCTTCTGCCTCCTGGAGG
  CGGCCCTGGCCGCCGAGGTGAAGAAACCTGCAGCCGCAGCAGCTCCTGGCACTGCGGAGA
  AGTTGAGCCCCAAGGCGGCCACGCTTGCCGAGCGCAGCGCCGGCCTGGCCTTCAGCTTGT
  ACCAGGCCATGGCCAAGGACCAGGCAGTGGAGAACATCCTGGTGTCACCCGTGGTGGTGG
  CCTCGTCGCTGGGGCTCGTGTCGCTGGGCGGCAAGGCGACCACGGCGTCGCAGGCCAAGG
  CAGTGCTGAGCGCCGAGCAGCTGCGCGACGAGGAGGTGCACGCCGGCCTGGGCGAGCTGC
  TGCGCTCACTCAGCAACTCCACGGCGCGCAACGTGACCTGGAAGCTGGGCAGCCGACTGT
  ACGGACCCAGCTCAGTGAGCTTCGCTGATGACTTCGTGCGCAGCAGCAAGCAGCACTACA
  ACTGCGAGCACTCCAAGATCAACTTCCGCGACAAGCGCAGCGCGCTGCAGTCCATCAACG
  AGTGGGCCGCGCATACCACCGACAGCNAGCTGCCCGATGTCACCAAGGACGTGGAGCGCA
  CGGACGGCGCCCTGTTAGTCAACGCCATGTTCTTCAGCCACACTGGGATGAGAAATTCAC
  CCACAGATGGTGGGACACCGTGGCTTTCTGGTGACTCGGTCCTTACCGTGGGTGTCATGA
  TGATGCACCGGACAGCCTCTACACTACTACGACGACGAGAAGAATAGCTGCAAATCGTGG
  AGATGCCCCTGGCCACAAGCTCTCN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001235 unedited
  TTATGGAACGCGGCCCGCTTTTCTAGAAGTCGAAGTTTTTTCCTTTTTTTTTTTGGCATT
  GGGAAAGTTTTATTGAAAAAAAAAAGTACAAATAAGTTCTTGGATTGATAGCAACAAAGG
  CTCATGTTCCCCCTTCCCTCCCTATCTTTGAAGAACTAAAAAAGGAAGAAACAAAACAAA
  AAGTTCATCCCCACAACGCCAGACACGATGCTTCTTGACCAGAGTCTGCCCAGAAGCCCC
  TCCTGGGAGCTCCTTCTCAATCCGCCTCACTGCGGCCAGGTCATTCTGGGGGTGCCTGGT
  CCCAGGGGCTGCAGCACCTAGTTTTATAGTTGGGAGAGGTTGGGATAGAGCTGGGGAGGC
  AGCTGAGGTGTTTTAGTGTCAGAAGAGGGCTGGCTGACCCCCCTAGCTCAATTTGGTCTC
  ACAGGTGAGAAGGTACCTGGCTATAAATATGAATACTGATTGAGGCAGGCTTGATCTGGG
  ACTTTCAGGGCAGGCAGAATGACTATGGCCCAGGTCCCACAGGATGTGGAGTTTCCGGGC
  TCAGCATCATGGTATCTGGGGCCCATGTCCAAGTGGAGTGAATTCTAGCTGGGAAGGTCC
  GCTCAAGCACACGGGAACCCCGGTCTGCTTTTCCACCCCCACCCCATGGAGTATCCAAGG
  CTGGTACCTCACCTTCCCCCCAACCCCATAGCACCCATGTGTCTCAGGAGCCTTTGGATG
  CCTCCTGCCATCCTGTGTGCACCCCTGAGCCCCTATACTCGGCTTGCATCTTGTCACCCC
  TTAAGCCGGACCAGCCGCCCAATGGATAACAGGAAGCCGCTTTTGTTCGTCCCGACCTAC
  GAAGAAGAAAGGCGGGGGCCGCGTATAACAGCTTGGAGGCTGGCCAACTCCTCCGCCCGC
  AAAGGCCCTGCCAAAGGGTTGCCATCTGTGGCCACTCAAGGCGGGGGGTGGAACAGCTGT
  CCAGTACAGGGCCTTTTGCTGAATGGGGAAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001235
• Insert Size: 2250 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001235.2, NP_001226.2
• RefSeq Size: 2208 bp
• RefSeq ORF: 1257 bp
• Locus ID: 871
• UniProt ID: P50454
• Cytogenetics: 11q13.5
• Domains: SERPIN
• Protein Families: Druggable Genome
• Gene Summary: This gene encodes a member of the serpin superfamily of serine proteinase inhibitors. The encoded protein is localized to the endoplasmic reticulum and plays a role in collagen biosynthesis as a collagen-specific molecular chaperone. Autoantibodies to the encoded protein have been found in patients with rheumatoid arthritis. Expression of this gene may be a marker for cancer, and nucleotide polymorphisms in this gene may be associated with preterm birth caused by preterm premature rupture of membranes. Alternatively spliced transcript variants have been observed for this gene, and a pseudogene of this gene is located on the short arm of chromosome 9. [provided by RefSeq, May 2011]
  Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.