CSNK1G2 (NM_001319) Human Untagged Clone

CAT#: SC119302

CSNK1G2 (untagged)-Human casein kinase 1, gamma 2 (CSNK1G2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CSNK1G2
• Synonyms: CK1g2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_001319 edited
  ATGGATTTTGACAAGAAAGGAGGGAAAGGGGAGACGGAGGAGGGCCGGAGAATGTCCAAG
  GCCGGCGGGGGCCGGAGCAGCCACGGCATCCGGAGCTCGGGGACCAGCTCGGGGGTCCTG
  ATGGTGGGCCCCAACTTCCGCGTCGGCAAGAAGATCGGCTGCGGCAACTTCGGGGAGCTC
  CGCCTAGGAAAGAATCTCTATACAAATGAATACGTGGCTATCAAATTGGAGCCGATCAAG
  TCCCGGGCCCCGCAGCTGCACCTGGAGTACCGGTTCTACAAGCAGCTCAGCGCCACAGAG
  GGCGTCCCTCAGGTCTACTACTTCGGTCCGTGCGGGAAGTACAACGCCATGGTGCTGGAG
  CTGCTGGGGCCCAGCCTGGAGGACCTGTTCGACCTGTGCGACCGGACCTTCACGCTCAAG
  ACGGTGCTGATGATCGCCATCCAGCTGATCACGCGCATGGAGTATGTGCACACCAAGAGC
  CTAATCTACCGGGACGTGAAGCCCGAGAACTTCCTGGTGGGCCGCCCGGGGACCAAGCGG
  CAGCATGCCATCCACATCATCGACTTCGGGCTGGCCAAGGAGTACATCGACCCCGAGACC
  AAGAAGCACATCCCGTACCGCGAGCACAAGAGCCTGACGGGCACGGCGCGCTACATGAGC
  ATCAACACGCACCTGGGCAAGGAGCAGAGCCGCCGCGACGACCTGGAGGCGCTGGGCCAC
  ATGTTCATGTACTTCCTGCGCGGCAGCCTCCCCTGGCAGGGGCTCAAGGCCGACACGCTC
  AAGGAGCGGTACCAGAAGATCGGGGACACCAAACGCGCCACGCCCATCGAGGTGCTCTGC
  GAGAACTTCCCAGAGGAGATGGCCACGTACCTGCGCTATGTGCGGCGCCTGGACTTCTTC
  GAGAAGCCCGACTATGACTACCTGCGGAAGCTCTTCACCGACCTCTTCGACCGCAGTGGC
  TTCGTGTTCGACTATGAGTACGACTGGGCCGGGAAGCCCCTGCCGACCCCCATCGGCACC
  GTCCACACCGACCTGCCCTCCCAGCCTCAGCTCCGGGACAAAACCCAGCCGCACAGCAAA
  AACCAGGCGTTGAACTCCACCAACGGGGAGCTGAATGCGGACGACCCCACGGCCGGCCAC
  TCCAACGCCCCGATCACAGCGCCTGCAGAGGTGGAGGTGGCCGATGAAACCAAATGCTGC
  TGTTTCTTCAAGAGGAGAAAGAGAAAATCGCTGCAGCGACACAAGTGA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001319 unedited
  TTAACGGCATTATTGTATACGACTCACTAAGGCGGCCCGCGAATTCGCACGAGGCAGAAT
  GTCTCCTGCCCCCGAGAGCGACCCCGAGCCCCTGAGAAGAGCAGCGCGGCCTGGCCGGCC
  CGAACGCCTGCGTCTCAGTAGCTGGGAGCCACGGGCCCACGCCCGCCCACCGGCCGCAGT
  GATGTTCTAGCCACAGAGGAGCCAAGACCTCAGGTTTCCAGAGACTTGGGATTTGCACGG
  CAGCAGAGTCACCGTGGAGAGGCCAGGGTATCACAAACTTATGGATTTTGACAAGAAAGG
  AGGGAAAGGGGAGACGGAGGAGGGCCGGAGAATGTCCAAGGCCGGCGGGGGCCGGAGCAG
  CCACGGCATCCGGAGCTCGGGGACCAGCTCGGGGGTCCTGATGGTGGGCCCCAACTTCCG
  CGTCGGCAAGAAGATCGGCTGCGGCAACTTCGGGGAGCTCCGCCTAGGAAAGAATCTCTA
  TACAAATGAATACGTGGCTATCAAATTGGAGCCGATCAAGTCCCGGGCCCCGCAGCTGCA
  CCTGGAGTACCGGTTCTACAAGCAGCTCAGCGCCACAGAGGGCGTCCCTCAGGTCTACTA
  CTTCGGTCCGTGCGGGAAGTACAACGCCATGGTGCTGGAGCTGCTGGGGCCCAGCCTGGA
  GGACCTGTTCGACCTGTGCGACCGGACCTTCACGCTCAAGACGGTGCTGATGATCGCCAT
  CCAGCTGATCACGCGCATGGAGTATGTGCACACCAAGAGCCTAATCTACCGGGACGTGAA
  GCCCGAGAACTCCCTGGNTGGCCGCCCGGGGACCAAGCGGCAGCATGCCATCCACATCAT
  CGACTTCGGGCTGGCCAAGGAGTACATCGACCCCGAGACCAGAGCACATCCCGTACCGCG
  AGCACNAGAGCCTGACGGGCACGGCGCGCT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001319 unedited
  GCGGCCGCAATCTAGNATCGAGTTTTTTTTTTTTTTTTTTCCTTGTTTTAAATAGTTAAT
  GCTCTTGGAGGGGAGGACTAGACACAGGTGTAGAAATTTCGGCCAAAGTCTTACATGAAG
  GAAGTGACCCCACGTCCCGGGGGTGGGGCCGCCTGAGCCAGGCGCGGCCCCTGCAGTCTG
  CGTTCTGGCTTCCAGCCAGGGTCTGGCCCTGGGCCGCTGTGGGTGGGCCCCGAGGGCCTC
  GCACAAGGTCGCGCCCCAAGGGGCTGCACGGAGAAGATTCAGGGGGCTGCACGCGCCCAG
  GGTCACTTGTGTCGCTGCAGCGATTTTCTCTTTCTCCTCTTGAAGAAACAGCAGCATTTG
  GTTTCATCGGCCACCTCCACCTCTGCAGGCGCTGTGATCGGGGCGTTGGAGTGGCCGGCC
  GTGGGGTCGTCCGCATTCAGCTCCCCGTTGGTGGAGTTCAACGCCTGGTTTTTGCTGTGC
  GGCTGGGTTTTGTCCCGGAGCTGAGGCTGGGAGGGCAGGTCGGTGTGGACGGTGCCGATG
  GGGGTCGGCAGGGGCTTCCCGGCCCAGTCGTACTCATAGTCGAACACGAAGCCACTGCGG
  TCGAAGAGGTCGGTGAAGAGCTTCCGCAGGTAGTCATAGTCGGGCTTCTCGAAGAAGTCC
  CAGCGCCGCACATAGCGCAGGTACGTGGCCATCTCCTCTGGGAAGTTCTCGCAGAGCACC
  CTCGATGGGCGTGGCGCGTTTGTTGTCCCCGATCTTCTGGTACGCTCCTTGAGCGTGTCG
  CCCTTGACCCCTGCCAGGGAAGCTGCCGNCCAGGAGTACATGAACATGTGGCCCACGGCT
  CCAGTTGTCGCGGCGGTTCTGTTCTTGCCAGTGGCGTGTGAAGCTATGTAAGGCGCCGGC
  CCGTAAGGCTTTGGTCCTCCGATCGGGATGGCCTTTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001319
• Insert Size: 1800 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001319.5, NP_001310.2
• RefSeq Size: 2446 bp
• RefSeq ORF: 1248 bp
• Locus ID: 1455
• UniProt ID: P78368
• Cytogenetics: 19p13.3
• Domains: pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Hedgehog signaling pathway
• Gene Summary: Serine/threonine-protein kinase. Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates COL4A3BP/CERT, MTA1 and SMAD3. Involved in brain development and vesicular trafficking and neurotransmitter releasing from small synaptic vesicles. Regulates fast synaptic transmission mediated by glutamate. SMAD3 phosphorylation promotes its ligand-dependent ubiquitination and subsequent proteasome degradation, thus inhibiting SMAD3-mediated TGF-beta responses. Hyperphosphorylation of the serine-repeat motif of COL4A3BP/CERT leads to its inactivation by dissociation from the Golgi complex, thus down-regulating ER-to-Golgi transport of ceramide and sphingomyelin synthesis. Triggers PER1 proteasomal degradation probably through phosphorylation.[UniProtKB/Swiss-Prot Function]