DUSP9 (NM_001395) Human Untagged Clone

CAT#: SC119259

DUSP9 (untagged)-Human dual specificity phosphatase 9 (DUSP9)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DUSP9
• Synonyms: MKP-4; MKP4
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001395, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGAGGGTCTGGGCCGCTCGTGCCTGTGGCTGCGTCGGGAGCTGTCGCCCCCGCGGCCGCGGCTCCTGC
  TCCTGGACTGCCGCAGCCGCGAGCTGTACGAGTCGGCGCGCATCGGTGGGGCGCTGAGCGTGGCCCTGCC
  GGCGCTCCTGCTGCGCCGCCTGCGGAGGGGCAGCCTGTCGGTGCGCGCGCTCCTGCCTGGGCCGCCGCTG
  CAGCCGCCCCCGCCTGCCCCCGTGCTCCTGTACGACCAGGGCGGGGGCCGGCGCCGGCGCGGGGAGGCCG
  AGGCCGAGGCCGAGGAGTGGGAGGCCGAGTCGGTGCTGGGCACCCTGCTGCAGAAGCTGCGAGAGGAAGG
  CTACCTGGCCTACTACCTCCAGGGAGGCTTCAGCAGATTCCAGGCCGAGTGCCCTCACCTGTGTGAGACC
  AGCCTTGCTGGCCGTGCCGGCTCCAGCATGGCGCCGGTGCCCGGTCCAGTGCCCGTGGTGGGGTTGGGCA
  GCCTGTGCCTGGGCTCCGACTGCTCTGATGCGGAATCCGAGGCTGACCGCGACTCCATGAGCTGTGGCCT
  GGATTCGGAGGGTGCCACACCCCCACCAGTGGGGCTGCGGGCATCCTTCCCTGTCCAGATCCTGCCCAAC
  CTCTATCTGGGCAGTGCCCGGGATTCCGCCAATTTGGAGAGCCTGGCCAAACTGGGCATCCGCTACATCC
  TCAATGTCACCCCCAACCTCCCAAACTTCTTCGAGAAGAATGGTGACTTTCACTACAAGCAGATCCCCAT
  CTCCGACCACTGGAGCCAGAACCTGTCGCGGTTCTTTCCGGAGGCCATTGAGTTCATTGATGAGGCCTTG
  TCCCAGAACTGCGGGGTGCTCGTCCACTGCTTGGCGGGGGTCAGCCGTTCTGTCACCGTCACTGTGGCCT
  ACCTCATGCAGAAGCTCCACCTCTCTCTCAACGATGCCTATGACCTGGTCAAGAGGAAGAAGTCTAACAT
  CTCCCCCAACTTCAACTTCATGGGGCAGTTGCTGGACTTTGAGCGCAGCTTGCGGCTGGAGGAGCGCCAC
  TCGCAGGAGCAGGGCAGTGGGGGGCAGGCATCTGCGGCCTCCAACCCGCCCTCCTTCTTCACCACCCCCA
  CCAGTGATGGCGCCTTCGAGCTGGCCCCCACCTAG
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001395 unedited
  GTTCCCATTTGTATACGACTCACTATAGGGCGGCCGCGAATTCGCACGAGGAGAACCGAG
  CAGAGCGGAGCGCCCGTGGTCCAGCGTGTAGGGAGCCGATCGCCCATGGAGGGTCTGGGC
  CGCTCGTGCCTGTGGCTGCGTCGGGAGCTGTCGCCCCCGCGGCCGCGGCTCCTGCTCCTG
  GACTGCCGCAGCCGCGAGCTGTACGAGTCGGCGCGCATCGGTGGGGCGCTGAGCGTGGCC
  CTGCCGGCGCTCCTGCTGCGCCGCCTGCGGAGGGGCAGCCTGTCGGTGCGCGCGCTCCTG
  CCTGGGCCGCCGCTGCAGCCGCCCCCGCCTGCCCCCGTGCTCCTGTACGACCAGGGCGGG
  GGCCGGCGCCGGCGCGGGGAGGCCGAGGCCGAGGCCGAGGAGTGGGAGGCCGAGTCGGTG
  CTGGGCACCCTGCTGCAGAAGCTGCGAGAGGAAGGCTACCTGGCCTACTACCTCCAGGGA
  GGCTTCAGCAGATTCCAGGCCGAGTGCCCTCACCTGTGTGAGACCAGCCTTGCTGGCCGT
  GCCGGCTCCAGCATGGCGCCGGTGCCCGGTCCAGTGCCCGTGGTGGAGTTGGGCAGCCTG
  TGCCTGGGCTCCGACTGCTCTGNATGCGAATCCGNAGCTGACCGCGACTCCATGAGCTGT
  GGCCTGGATTCNGAGGGTGCCACACCCNCACCAGTGGGGCTGCGGGCATCCTTCCCTGTC
  CAGATCCTGCCCCACCTCTATCTGGGCAGTGCCCGGGATTCGNCAATNTGGAGAGCCTGG
  NCAAACTGGGCATNCGCTACATNNCTCATGTCACCCCCNACCTNCCCAACTTCTTCGAGA
  AGAATGGTGACTTTCACTANCAGCAGATCCCCATCTCCGACCACTGGNAGCAGAACCTGG
  TCGCGTNTCTTTCCGGAGCCACTGAGTTCATTGATGAGGCCTTGTCCANNACCTGCGGGT
  GCT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001395 unedited
  AAGACCTAAATAACTGTTTNTTCCACTAAGGNAACAATTAAGNAAAGCAACTATGATATG
  GATTACAAAAACAAACAAGCATAGATCCTCTCCAGGCTCCAGGGTGAGACGGCCCCACGC
  TGCAAAGACTGCCAGCCCTGGGCTGGGTGCATCAGTGGGCTAGCATGTGTGTGTGAGCGC
  CGACACGTGCGCACACACAAGCACACACACGCACACCCGCCACCCACAGGGGTGTGGACA
  GAAATGAATGACTGTGTGTGTCTGCAGCTTGGCCCCTTCCCCACCCCCCAAAGTAGCAGC
  GGCTGTGTGGGCCCCGCAGCAGCAAAAGTGAGGCCTTCTTCTTCCCCTCCCCCTCCGCAG
  CTCTCGCTCTGAGACCCCATCTCCTCCGGCGGTAGTTTGGCCACCCTCACTCACAAAGAG
  CCCCAGTGATCCCGTCAAGACATCTGGGGGAAGAGGAAAAAAAAAAAAACAAAACAGCCC
  ACCAGATGTGTGGCCGGGAACCTGCCCAGTAGCTCCTAGGCCATGGATGTGGGCGGCCAC
  TGCACCTCTGGGTCCCCATCCCCTGGGAGGCCCCAGCCCCGAGAGGCCCAAGCCCTTGGT
  GGAGAAACAGGGAGGTGACAACACCAGTGATGGCAAACGCCTCTCCCCCAAGCAGCCACT
  GGGATCCAGTGCAACGAGGTGGCACACACCCTTCCTGAAGGGGGAAGCGCAGGGAGGCGC
  AGGGCCTAGCCGAGCTGGCTGAGCACCCAGGGCCGGGACCTGTGCCTGGACACCACCTGN
  AGAGGGAACCCCCACCCCCATCCCGCATAGAAAAAGTAACAGTCGCACAAGAGGCAGGCT
  CCGAGCGTGGTCCCGCCATAGGCACGTTGATTAGGACGGCAGCCCGCGTGAGGTATTGCG
  CTCTCCCCCAGCACCCTGGTGAG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001395
• Insert Size: 2450 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001395.1, NP_001386.1
• RefSeq Size: 2303 bp
• RefSeq ORF: 1155 bp
• Locus ID: 1852
• UniProt ID: Q99956
• Cytogenetics: Xq28
• Domains: DSPc, RHOD
• Protein Families: Phosphatase
• Protein Pathways: MAPK signaling pathway
• Gene Summary: The protein encoded by this gene is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which is associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene product shows selectivity for members of the ERK family of MAP kinases and is localized to the cytoplasm and nucleus. Aberrant expression of this gene is associated with type 2 diabetes and cancer progression in several cell types. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016]
  Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Both variants 1 and 2 encode the same protein.