Alkaline Phosphatase (ALPP) (NM_001632) Human Untagged Clone

CAT#: SC119167

ALPP (untagged)-Human alkaline phosphatase, placental (ALPP)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Alkaline Phosphatase
• Synonyms: ALP; ALPI; IAP; PALP; PLAP; PLAP-1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119167 sequence for NM_001632 edited (data generated by NextGen Sequencing)
  ATGCTGGGGCCCTGCATGCTGCTGCTGCTGCTGCTGCTGGGCCTGAGGCTACAGCTCTCC
  CTGGGCATCATCCCAGTTGAGGAGGAGAACCCGGACTTCTGGAACCGCGAGGCAGCCGAG
  GCCCTGGGTGCCGCCAAGAAGCTGCAGCCTGCACAGACAGCCGCCAAGAACCTCATCATC
  TTCCTGGGCGATGGGATGGGGGTGTCTACGGTGACAGCTGCCAGGATCCTAAAAGGGCAG
  AAGAAGGACAAACTGGGGCCTGAGATACCCCTGGCCATGGACCGCTTCCCATATGTGGCT
  CTGTCCAAGACATACAATGTAGACAAACATGTGCCAGACAGTGGAGCCACAGCCACGGCC
  TACCTGTGCGGGGTCAAGGGCAACTTCCAGACCATTGGCTTGAGTGCAGCCGCCCGCTTT
  AACCAGTGCAACACGACACGCGGCAACGAGGTCATCTCCGTGATGAATCGGGCCAAGAAA
  GCAGGGAAGTCAGTGGGAGTGGTAACCACCACACGAGTGCAGCACGCCTCGCCAGCCGGC
  ACCTACGCCCACACGGTGAACCGCAACTGGTACTCGGACGCCGACGTGCCTGCCTCGGCC
  CGCCAGGAGGGGTGCCAGGACATCGCTACGCAGCTCATCTCCAACATGGACATTGACGTG
  ATCCTAGGTGGAGGCCGAAAGTACATGTTTCGCATGGGAACCCCAGACCCTGAGTACCCA
  GATGACTACAGCCAAGGTGGGACCAGGCTGGACGGGAAGAATCTGGTGCAGGAATGGCTG
  GCGAAGCGCCAGGGTGCCCGGTATGTGTGGAACCGCACTGAGCTCATGCAGGCTTCCCTG
  GACCCGTCTGTGACCCATCTCATGGGTCTCTTTGAGCCTGGAGACATGAAATACGAGATC
  CACCGAGACTCCACACTGGACCCCTCCCTGATGGAGATGACAGAGGCTGCCCTGCGCCTG
  CTGAGCAGGAACCCCCGCGGCTTCTTCCTCTTCGTGGAGGGTGGTCGCATCGACCATGGT
  CATCATGAAAGCAGGGCTTACCGGGCACTGACTGAGACGATCATGTTCGACGACGCCATT
  GAGAGGGCGGGCCAGCTCACCAGCGAGGAGGACACGCTGAGCCTCGTCACTGCCGACCAC
  TCCCACGTCTTCTCCTTCGGAGGCTACCCCCTGCGAGGGAGCTCCATCTTCGGGCTGGCC
  CCTGGCAAGGCCCGGGACAGGAAGGCCTACACGGTCCTCCTATACGGAAACGGTCCAGGC
  TATGTGCTCAAGGACGGCGCCCGGCCGGATGTTACCGAGAGCGAGAGCGGGAGCCCCGAG
  TATCGGCAGCAGTCAGCAGTGCCCCTGGACGAAGAGACCCACGCAGGCGAGGACGTGGCG
  GTGTTCGCGCGCGGCCCGCAGGCGCACCTGGTTCACGGCGTGCAGGAGCAGACCTTCATA
  GCGCACGTCATGGCCTTCGCCGCCTGCCTGGAGCCCTACACCGCCTGCGACCTGGCGCCC
  CCCGCCGGCACCACCGACGCCGCGCACCCGGGGCGGTCCGTGGTCCCCGCGTTGCTTCCT
  CTGCTGGCCGGGACCCTGCTGCTGCTGGAGACGGCCACTGCTCCCTGA
  
  Clone variation with respect to NM_001632.3
  597 c=>g
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001632 unedited
  NGAGTTAGCATTTGTATCCGACTCCTATAGGCGGCCGCGCAATTCGCACGAGCTCATACT
  CCATGCCCANAATTCCTGCCTCGCCACTGTCCTGCTGCCCTCCAGACATGCTGGGGCCCT
  GCATGCTGCTGCTGCTGCTGCTGCTGGGCCTGAGGCTACAGCTCTCCCTGGGCATCATCC
  CAGTTGAGGAGGAGAACCCGGACTTCTGGAACCGCGAGGCAGCCGAGGCCCTGGGTGCCG
  CCAAGAAGCTGCAGCCTGCACAGACAGCCGCCAAGAACCTCATCATCTTCCTGGGCGATG
  GGATGGGGGTGTCTACGGTGACAGCTGCCAGGATCCTAAAAGGGCAGAAGAAGGACAAAC
  TGGGGCCTGAGATACCCCTGGCCATGGACCGCTTCCCATATGTGGCTCTGTCCAAGACAT
  ACAATGTAGACAAACATGTGCCAGACAGTGGAGCCACAGCCACGGCCTACCTGTGCGGGG
  TCAAGGGCAACTTCCAGACCATTGGCTTGAGTGCAGCCGCCCGCTTTAACCAGTGCAACA
  CGACACGCGGCAACGAGGTCATCTCCGTGATGAATCGGGCCAAGAAAGCAGGGAAGTCAG
  TGGGAGTGGTAACCACCACACGAGTGCAGCACGCCTCGCCAGCCGGCACCTACGCCCACA
  CGGTGAACCGCAACTGGTACTNCGACGCCGACGTGCCTGCCTCGGCCCCGCCAGGAGGGT
  GCCANGACATCGCTACGCAGCTCATCTCCCACATGGACATTGACGTGATCCCTAGTGGGA
  GCCGANAGTACATGTTTCGCATGGGAACCNCAGACCCTGAGTACCCAGATGACTACAGCC
  CAAGTGGNACCAGNCTGNACGGNAGAAATCTGTGCAGNAATGGCTGNCGAANCCCCAGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001632 unedited
  CATTGGCGATGGCACTTCCAGGNCCAGNATAGGCACTGGGTGAGGGTCACAGGGCTGCCA
  CCCGGGATCTGTTCAGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTT
  TTTTTTTTTTTATTATTTATTTATTTTTAAAATTTATTTGTTTATTTTTGAGGCAGAATC
  TCGCTCTGTCGCCCAGGCTGCAGTGCAGTGGCATGACCTCGGCTCACTGCAATCTCCGCC
  GCCCGGGTTCAAGCGATTATCCTGCTTCAGCCTCCTGGGTAGCTGGGACTACAGGCGCAT
  AGATAAGGGGTTTCACCGTGTTATCCTAGATGGTCTCCTTCTCCTGACCTATGGTTCCGC
  TTACTTTTGTCTCGCAAAAGTGCTGGGATTACAAACACTTCCTTTTATTATTGCTTTGTC
  TCCCAAGAGAGCAGATGCCCTGGGGAGGCAGGGGAGTCTTCCTTCTTGAGAAATCTATGC
  CCAGTGTCCATATGTCTGGAGAGGGACCTTTGGCTCTCGACCAGTGTCCTGCACCCAGGG
  CTTCCCAGGAGCTCCTCGGATCTCAGTTGGGTGCTGAAATTGGCATGTGGAGGTATGGAA
  CGTCTGANAAGTTGAGATTTGGGGCAAGCCTTTGCCATGAGGCAGAGGGCCCCCTGGGGG
  GGTACAGGGGTGTGGAATTAGCTTAGTGGGGGGCAGCCGAAGGCTGTGTCAAAATCCCCC
  TGGTGATCCCCACCCCAGGAGCCTCCTTGGCCAGGAGGGTGGCAAGAAACCACCCTTTCC
  CAAGCCTCCTGAAAATCCCGAGTCCTCAGTTCCCACAGTGATTCAGGACTGAACCAAAAT
  TCACGGGCGCGGGGTCTGGTTGCTTCTTTTTCCCAGAAAGGACCCTGCCTGGACCCTTGG
  CTTTTAGTGGTGGCTGGATGCCCTGACCCCTTCTTTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001632
• Insert Size: 1608 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001632.2, NP_001623.2
• RefSeq Size: 2747 bp
• RefSeq ORF: 1608 bp
• Locus ID: 250
• UniProt ID: P05187
• Cytogenetics: 2q37.1
• Domains: alk_phosphatase
• Protein Pathways: Folate biosynthesis, Metabolic pathways
• Gene Summary: The protein encoded by this gene is an alkaline phosphatase, a metalloenzyme that catalyzes the hydrolysis of phosphoric acid monoesters. It belongs to a multigene family composed of four alkaline phosphatase isoenzymes. The enzyme functions as a homodimer and has a catalytic site containing one magnesium and two zinc ions, which are required for its enzymatic function. One of the main sources of this enzyme is the liver, and thus, it's one of several indicators of liver injury in different clinical conditions. In pregnant women, this protein is primarily expressed in placental and endometrial tissue, however, strong ectopic expression has been detected in ovarian adenocarcinoma, serous cystadenocarcinoma, and other ovarian cancer cells. [provided by RefSeq, Aug 2020]