Calmodulin (CALM2) (NM_001743) Human Untagged Clone

CAT#: SC119076

CALM2 (untagged)-Human calmodulin 2 (phosphorylase kinase, delta) (CALM2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Calmodulin
• Synonyms: CALM; CALML2; caM; CAM1; CAM3; CAMC; CAMII; CAMIII; LQT15; PHKD; PHKD2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001743 edited
  GAATTCGGCACGAGGGCGAGCTGAGTGGTTGTGTGGTCGCGTCTCGGAAACCGGTAGCGC
  TTGCAGCATGGCTGACCAACTGACTGAAGAGCAGATTGCAGAATTCAAAGAAGCTTTTTC
  ACTATTTGACAAAGATGGTGATGGAACTATAACAACAAAGGAATTGGGAACTGTAATGAG
  ATCTCTTGGGCAGAATCCCACAGAAGCAGAGTTACAGGACATGATTAATGAAGTAGATGC
  TGATGGTAATGGCACAATTGACTTCCCTGAATTTCTGACAATGATGGCAAGAAAAATGAA
  AGACACAGACAGTGAAGAAGAAATTAGAGAAGCATTCCGTGTGTTTGATAAGGATGGCAA
  TGGCTATATTAGTGCTGCAGAACTTCGCCATGTGATGACAAACCTTGGAGAGAAGTTAAC
  AGATGAAGAAGTTGATGAAATGATCAGGGAAGCAGATATTGATGGTGATGGTCAAGTAAA
  CTATGAAGAGTTTGTACAAATGATGACAGCAAAGTGAAGACCTTGTACAGAATGTGTTAA
  ATTTCTTGTACAAAATTGTTTATTTGCCTTTTCTTTGTTTGTAACTTATCTGTAAAAGGT
  TTCTCCCTACTGTCAAAAAAATATGCATGTATAGTAATTAGGACTTCATTCCTCCATGTT
  TTCTTCCCTTATCTTACTGTCATTGTCCTAAAACCTTATTTTAGAAAATTGATCAAGTAA
  CATGTTGCATGTGGCTTACTCTGGATATATCTAAGCCCTTCTGCACATCTAAACTTAGAT
  GGAGTTGGTCAAATGAGGGAACATCTGGGTTATGCCTTTTTTAAAGTAGTTTTCTTTAGG
  AACTGTCAGCATGTTGTTGTTGAAGTGTGGAGTTGTAACTCTGCGTGGACTATGGACAGT
  CAACAATATGTACTTAAAAGTTGCACTATTGCAAAACGGGTGTATTATCCAGGTACTCGT
  ACACTATTTTTTTGTACTGCTGGTCCTGTACCAGAAACATTTTCTTTTATTGTTACTTGC
  TTTTTAAACTTTGTTTAGCCACTTAAAATCTGCTTATGGCACAATTTGCCTCAAAATCCA
  TTCCAAGTTGTATATTTGTTTTCCAATAAAAAAATTACAATTTACCCAAAAAAAAAAAAA
  AAAAACTCGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001743 unedited
  ATTTTGTAATACGACTTANCTATAGGGCGGCCGCGAATTCGCACGAGGGCGAGCTGATTG
  GTTGTGTGGTCGCGTCTCGGAAACCGGTAGCGCTTGCAGCATGGCTGACCAACTGACTGA
  AGAGCAGATTGCAGAATTCAAAGAAGCTTTTTCACTATTTGACAAAGATGGTGATGGAAC
  TATAACAACAAAGGAATTGGGAACTGTATTTATATCTCTTGGGCAGAATCCCACAGAAGC
  AGAGTTACAGGACATGATTAATGAAGTAGATGCTGATGGTAATGGCACAATTGACTTCCC
  TGAATTTCTGACAATGATGGCAAGAAAAATGAAAGACACAGACAGTGAAGAAGAAATTAG
  AGAAGCATTCCGTGTGTTTGATAAGGATGGCAATGGCTATATTAGTGCTGCAGAACTTCG
  CCATGTGATGACAAACCTTGGAGAGAAGTTAACAGATGAAGAAGTTGATGAAATGATCAG
  GGAAGCAGATATTGATGGTGATGGTCAAGTAAACTATGAAGAGTTTGTACAAATGATGAC
  AGCAAAGTGAAGACCTTGTACAGAATGTGTTAAATTTCTTGTACAAAATTGTTTATTTGC
  CTTTTCTTTGTTTGTAACTTATCTGTAAAAGGTTTCTCCCTACTGTCAAAAAAATATGCA
  TGTATAGTAATTAGGACTTCATTCCTCCATGTTTTCTTCCCTTATCTTACTGTCATTGTC
  CTAAAACCTTATTTTAGAANATTGATCAAGTAACATGTTGCATGTGGCTTACTCTGGATA
  TATCTAAGCCCTTCTGCACATCTAAACTTAAATGGAGNTGGTCAAATGAGGGAAACATCT
  GGNTATGCCTTTTTTTAAGTAAGTTTCTTAGGAACTGTCACCATGNTGTTGTTGAAGTGT
  GGAGTTGAACTCTGCGTGGACTATGGCAGCCACCATATGTACTTAAAAGTGCACTATGCA
  AACGGTTGATNATCCAGGACTT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001743 unedited
  TTTCCCCCCCCCTCCNCCCCTCCNCTTCCCTTACCTTGTAAGCGGCCGCAATCTACATCG
  AGTTTTTTTTTTTTTTTTTTGGGTAAATTGTAATTTTTTTATTGGAAAACAAATATACAA
  CTTGCGAAGGATTTTGAGGCAAATTGTGCCATAAGCAGATTTTAAGTGGCTAAACAAAGT
  TTAAAAAGCAAGTAACAATAAAAGAAAATGTTTCTGGTACAGGACCAGCAGTACAAAAAA
  ATAGTGTACGAGTACCTGGATAATACACCCGTTTTGCAATAGTGCAACTTTTAAGTACAT
  ATTGTTGACTGTCCATAGTCCACGCAGAGTTACAACTCCACACTTCAACAACAACATGCT
  GACAGTTCCTAAAGAAAACTACTTTAAAAAAGGCATAACCCAGATGTTCCCTCATTTGAC
  CAACTCCATCTAAGTTTAGATGTGCAGAAGGGCTTATATATATCCAGAGTAAGCCACATG
  CAACATGTTACTTGATCAATTTTCTAAAATAAGGCTTTAGGACAATGACAGCAAGATAAG
  GGAAGAAAACATGGAGGAATGAAGCCCTAATTACTATACATGCATATTTTTTTGACAGTA
  GGGAGAAACCTTTTACAGATAAGTTACAAACACAGAAAAGGCAAATAAACAATTTTGTTC
  CAGAAAATTAACACATTCTGTACAAGTCCTTAACTTTGGTGTCATTATTTTGTACAAACT
  TTTTATTAGTTTACTTGACCATCACCATTAATATCTGCTTCCCTGATATTTCAATAAACT
  TTTAATCCGTTAACCTCTCTTCAAGGTTTTGTTATACAATTGCCCAAAGTTCTTTAACCA
  CTAATAATACCCATTGGCCTTCCCTATTCAAACACACCGGAAAGCCTCCCCTAATTCCTT
  TCTCAACGGCCTGGGCCTCTTCATTTTTCTTTGCCACTCTTTGCACAGAAT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001743
• Insert Size: 1160 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001743.3, NP_001734.1
• RefSeq Size: 1128 bp
• RefSeq ORF: 450 bp
• Locus ID: 805
• UniProt ID: P62158
• Cytogenetics: 2p21
• Domains: EFh
• Protein Families: Druggable Genome
• Protein Pathways: Alzheimer's disease, Calcium signaling pathway, Glioma, GnRH signaling pathway, Insulin signaling pathway, Long-term potentiation, Melanogenesis, Neurotrophin signaling pathway, Olfactory transduction, Oocyte meiosis, Phosphatidylinositol signaling system, Vascular smooth muscle contraction
• Gene Summary: This gene is a member of the calmodulin gene family. There are three distinct calmodulin genes dispersed throughout the genome that encode the identical protein, but differ at the nucleotide level. Calmodulin is a calcium binding protein that plays a role in signaling pathways, cell cycle progression and proliferation. Several infants with severe forms of long-QT syndrome (LQTS) who displayed life-threatening ventricular arrhythmias together with delayed neurodevelopment and epilepsy were found to have mutations in either this gene or another member of the calmodulin gene family (PMID:23388215). Mutations in this gene have also been identified in patients with less severe forms of LQTS (PMID:24917665), while mutations in another calmodulin gene family member have been associated with catecholaminergic polymorphic ventricular tachycardia (CPVT)(PMID:23040497), a rare disorder thought to be the cause of a significant fraction of sudden cardiac deaths in young individuals. Pseudogenes of this gene are found on chromosomes 10, 13, and 17. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Mar 2015]
  Transcript Variant: This variant (2) lacks an exon in the 5' coding region, and uses an alternate start codon compared to variant 1. The encoded isoform (2) has a shorter N-terminus compared to isoform 1, and is the same protein encoded by other calmodulin gene family members.