Creatine kinase M type (CKM) (NM_001824) Human Untagged Clone

CAT#: SC119007

CKM (untagged)-Human creatine kinase, muscle (CKM)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Creatine kinase M type
• Synonyms: CKMM; CPK-M; M-CK
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119007 sequence for NM_001824 edited (data generated by NextGen Sequencing)
  ATGCCATTCGGTAACACCCACAACAAGTTCAAGCTGAATTACAAGCCTGAGGAGGAGTAC
  CCCGACCTCAGCAAACATAACAACCACATGGCCAAGGTACTGACCCTTGAACTCTACAAG
  AAGCTGCGGGACAAGGAGACTCCATCTGGCTTCACTGTAGACGATGTCATCCAGACAGGA
  GTGGACAACCCAGGTCACCCCTTCATCATGACCGTGGGCTGCGTGGCTGGTGATGAGGAG
  TCCTACGAAGTTTTCAAGGAACTCTTTGACCCCATCATCTCGGATCGCCACGGGGGCTAC
  AAACCCACTGACAAGCACAAGACTGACCTCAACCATGAAAACCTCAAGGGTGGAGACGAC
  CTGGACCCCAACTACGTGCTCAGCAGCCGCGTCCGCACTGGCCGCAGCATCAAGGGCTAC
  ACGTTGCCCCCACACTGCTCCCGTGGCGAGCGCCGGGCGGTGGAGAAGCTCTCTGTGGAA
  GCTCTCAACAGCCTGACGGGCGAGTTCAAAGGGAAGTACTACCCTCTGAAGAGCATGACG
  GAGAAGGAGCAGCAGCAGCTCATCGATGACCACTTCCTGTTCGACAAGCCCGTGTCCCCG
  CTGCTGCTGGCCTCAGGCATGGCCCGCGACTGGCCCGACGCCCGTGGCATCTGGCACAAT
  GACAACAAGAGCTTCCTGGTGTGGGTGAACGAGGAGGATCACCTCCGGGTCATCTCCATG
  GAGAAGGGGGGCAACATGAAGGAGGTTTTCCGCCGCTTCTGCGTAGGGCTGCAGAAGATT
  GAGGAGATCTTTAAGAAAGCTGGCCACCCCTTCATGTGGAACCAGCACCTGGGCTACGTG
  CTCACCTGCCCATCCAACCTGGGCACTGGGCTGCGTGGAGGCGTGCATGTGAAGCTGGCG
  CACCTGAGCAAGCACCCCAAGTTCGAGGAGATCCTCACCCGCCTGCGTCTGCAGAAGAGG
  GGTACAGGTGGCGTGGACACAGCTGCCGTGGGCTCAGTATTTGACGTGTCCAACGCTGAT
  CGGCTGGGCTCGTCCGAAGTAGAACAGGTGCAGCTGGTGGTGGATGGTGTGAAGCTCATG
  GTGGAAATGGAGAAGAAGTTGGAGAAAGGCCAGTCCATCGACGACATGATCCCCGCCCAG
  AAGTAG
  
  Clone variation with respect to NM_001824.3
  369 t=>c;1119 t=>c
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001824 unedited
  TGTAATACGACTCACTATAGNNGGCGGCCGCGAAATTCGGCACGAGGGTCACCTCCAGGA
  TACAGACAGCCCCCCTTCAGCCCAGCCCAGCCAGGTCTCCTACACCGCCACCATGCCATT
  CGGTAACACCCACAACAAGTTCAAGCTGAATTACAAGCCTGAGGAGGAGTACCCCGACCT
  CAGCAAACATAACAACCACATGGCCAAGGTACTGACCCTTGAACTCTACAAGAAGCTGCG
  GGACAAGGAGACTCCATCTGGCTTCACTGTAGACGATGTCATCCAGACAGGAGTGGACAA
  CCCAGGTCACCCCTTCATCATGACCGTGGGCTGCGTGGCTGGTGATGAGGAGTCCTACGA
  AGTTTTCAAGGAACTCTTTGACCCCATCATCTCGGATCGCCACGGGGGCTACAAACCCAC
  TGACAAGCACAAGACTGACCTCAACCATGAAAACCTCAAGGGTGGAGACGACCTGGACCC
  CAACTACGTGCTCAGCAGCCGCGTCCGCACTGGCCGCAGCATCAAGGGCTACACGTTGCC
  CCCACACTGCTCCCGTGGCGAGCGCCGGGCGGTGGAGAAGCTCTCTGTGGAAGCTCTCAA
  CAGCCTGACGGNCGAGTTCAAAGGGAAGTACTACCCTTTGAAGAGCATGACCGAGAAGGA
  GCAGCAGCAGCTCATCGATGACCACTTTCTGTTCGACAAGCCCGTGTCCCCGCTGGTGTT
  GCCCTAAGCATGGCCCGGGACTGGCCCGAGCCCGTGGCATCTTGCACAATGACACAAGAG
  CTTCTGGTGTGGATGACGAAGGAGATCACCTCGGGTCATCTCATGGAAAAGGGGGCACAT
  GAAGGAGGTTTTCCGCCCTTTTGCGTAGGCTGCAAAGATTGAAGAAATCTTAAAAAGCTG
  GCACCCCTTTAGTGAACCAGCACTGTGTTACGGCTACCTGCCATCCACTG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001824 unedited
  ATAAGGACGCGGCCCCCATTCAAGGGCGGGTTTTTTTTTTTTTTTTTAGGCGCCCGATGC
  TTTTATTTATCGCTTTGCGTGGAGACAAAGCACAAGCTCCGAGTGTGCTGGGAGCTGTCC
  ATTAACTAGCGCTCCTGGTTGGGGCGGAGCTCTGGGAAAAGAAGACGACCCTGCCCGGGA
  GATATTTCACTTGGCCAGAATCCAGAGGATGGAGCCCATCGGTTGGAACTCTGGCTGAAA
  CTGGAACTCTGAGAAGGGCGGAGAGAGCCCCCAGGTGGGACTCTGGGACATGGGGCGGGG
  CGAGGAGTGAGGGACCAAGACTCTGGCGGGCCAGGCCCTCCGCCTGGCTGGGTTCCAGCC
  ATCGGAGGCAGGTGGGTGATGCGCCTGCTTGTGGGCGGAGGAGATGACGGCGATCGGAAT
  GGCCTCTTTCCGTCTTATCCTCCCGTTGCGCCATGCCCTTCCACCCCTGGACCATGGACT
  GAATCTGTTCTGGGTCGTACCCGCGTAGTCGATCCGTGTGGGGCGTCTCCGACGCTGGTC
  CGGGGGCGGCGTTTGGGCTATCCCGCTTAGCCCCGCTTCGGCAGGCCGGGGCCCGTGATT
  ATCTTTCCCGGACGCGCGCGCGTATGATAGAGGCCCCCGCATCGCCAACGCAGGTTGCAC
  GAATGCCTCGATATCCCGTCCGCGGGTCGGCTCCAGCCCATACATGCAAGGGTGCGCGAT
  TCCTTTATCGTCATCCGATGTTCCCCCGAGCGTTTCGGGTCCGCGTCGTTCCCCGTCGCG
  TGCAATGGATGGCCCCCTCGCGCTGCGGAACCACCTTAGAGATAGAGAGTGACGGGAAGT
  GGAGACTGGGGTGACTGCCTCGTACGGCAATGGCTTGGTACTTCTACAGCACAAGCCCAG
  GCTTGTCCGGTCCAAGTGTGGCCGCCCGGGTATACACTCTAAGAGCGGCACGCGGGTGCT
  ATGCTTGCTGGCTGCGCGGCCGGCCGTCCG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001824
• Insert Size: 1700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001824.2, NP_001815.2
• RefSeq Size: 1620 bp
• RefSeq ORF: 1146 bp
• Locus ID: 1158
• UniProt ID: P06732
• Cytogenetics: 19q13.32
• Domains: ATP-gua_Ptrans
• Protein Families: Druggable Genome
• Protein Pathways: Arginine and proline metabolism, Metabolic pathways
• Gene Summary: The protein encoded by this gene is a cytoplasmic enzyme involved in energy homeostasis and is an important serum marker for myocardial infarction. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in striated muscle as well as in other tissues, and as a heterodimer with a similar brain isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family. [provided by RefSeq, Jul 2008]