mu Crystallin (CRYM) (NM_001888) Human Untagged Clone

SKU SC118966

CRYM (untagged)-Human crystallin, mu (CRYM), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: mu Crystallin
• Synonyms: DFNA40; THBP
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118966 sequence for NM_001888 edited (data generated by NextGen Sequencing); ATGAGCCGGGTACCAGCGTTCCTGAGCGCGGCCGAGGTGGAGGAACACCTCCGCAGCTCC; AGCCTCCTCATCCCGCCTCTAGAGACGGCCCTGGCCAACTTCTCCAGCGGTCCCGAAGGA; GGGGTCATGCAGCCCGTGCGCACCGTGGTGCCGGTGACCAAGCACAGGGGCTACCTGGGG; GTCATGCCCGCCTACAGTGCTGCAGAGGATGCACTGACCACCAAGTTGGTCACCTTCTAC; GAGGACCGCGGCATCACCTCGGTCGTCCCTTCCCACCAGGCTACTGTGCTACTCTTTGAG; CCCAGCAATGGCACCCTGCTGGCGGTCATGGATGGAAATGTCATAACTGCAAAGAGAACA; GCTGCAGTTTCTGCCATTGCCACCAAGTTTCTGAAACCTCCCAGCAGTGAAGTGCTGTGC; ATCCTTGGGGCTGGGGTCCAGGCCTACAGCCATTATGAGATCTTCACAGAGCAGTTCTCC; TTTAAGGAGGTGAGGATATGGAACCGCACCAAAGAAAATGCAGAGAAGTTTGCAGACACA; GTGCAAGGAGAGGTACGGGTCTGTTCTTCGGTCCAGGAGGCTGTGGCAGGTGCAGATGTG; ATCATCACAGTCACCCTGGCAACAGAGCCCATTTTGTTTGGTGAATGGGTGAAGCCAGGG; GCTCACATCAATGCTGTTGGAGCCAGCAGACCTGACTGGAGAGAACTGGATGATGAGCTC; ATGAAAGAAGCTGTGCTGTACGTGGATTCCCAGGAGGCTGCCCTGAAGGAGTCTGGAGAT; GTCCTGCTGTCAGGGGCCGAGATCTTTGCTGAGCTGGGAGAAGTGATTAAGGGAGTGAAA; CCAGCCCACTGTGAGAAGACCACCGTGTTCAAGTCTTTGGGAATGGCAGTGGAAGACACA; GTTGCAGCCAAACTCATCTATGATTCCTGGTCATCTGGTAAATAA; ; Clone variation with respect to NM_001888.3;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001888 unedited; TAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGCGAGATGAGCCGGGTAC; CAGCGTTCCTGAGCGCGGCCGAGGTGGAGGAACACCTCCGCAGCTCCAGCCTCCTCATCC; CGCCTCTAGAGACGGCCCTGGCCAACTTCTCCAGCGGTCCCGAAGGAGGGGTCATGCAGC; CCGTGCGCACCGTGGTGCCGGTGACCAAGCACAGGGGCTACCTGGGGGTCATGCCCGCCT; ACAGTGCTGCAGAGGATGCACTGACCACCAAGTTGGTCACCTTCTACGAGGACCGCGGCA; TCACCTCGGTCGTCCCTTCCCACCAGGCTACTGTGCTACTCTTTGAGCCCAGCAATGGCA; CCCTGCTGGCGGTCATGGATGGAAATGTCATAACTGCAAAGAGAACAGCTGCAGTTTCTG; CCATTGCCACCAAGTTTCTGAAACCTCCCAGCAGTGAAGTGCTGTGCATCCTTGGGGCTG; GGGTCCAGGCCTACAGCCATTATGAGATCTTCACAGAGCAGTTCTCCTTTAAGGAGGTGA; GGATATGGAACCGCACCAAAGAAAATGCAGAGAAGTTTGCAGACACAGTGCAAGGAGAGG; TACGGGTCTGTTCTTCGGTCCAGGAGGCTGTGGCAGGTGCAGATGTGATCATCACAGTCA; CCCTGNCAACAGAGCCCATTTTGTTTGGTGAATGGGTGAAGCCAGGGGCTCACATCAATG; CTGTTGGAGCCAGCAGACCTGACTGGAGAGAACTGGATGATGAGCTCATGAAAGAAGCTG; TGCTGTACGTGGATTCCCAGNAGGCTGCCCTGAAAGAGTCTGGAGATGTCCTGCTGTCAG; GNGCCGAGATCTTTGCTGAGCTGGGAGAAGTGATTAAGGGAGTGAAACCAGCCCACTGTG; AGAAGACCACGTGNTNCAAGTCTTTGGAATGGCAGTGAAN;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001888 unedited; ATGTACCGCGGCACGCAATCTATAGTCGAGTTTTTTTTTTTTTTTTTTTTAACAATTGGA; ATGTGCTTTATTTCAGGGAAAATATAAAGGGAAATGAATGCTATTATAACTTGGTAGAAC; AGAAGAAATGGCTACCTAGCTTTGCTTTCCAACTACAAACATAAATGAGGATCTCAGCAT; TTAAGGTAAAACATGATAAGCACAAAAGGAGAGTTCACTGGGGACTGGACTCCCTCATTT; ACTCTAGAAATTATGAGAACCAGCAGCAATATTCCTCAAGCATCCATCTCAACATCAAGT; TCCTTTGTTTTATTTACCAGATGACCAGGAATCATAGATGAGTTTGGCTGCAACTGTGTC; TTCCACTGCCATTCCCAAAGACTTGAACACGGTGGTCTTCTCACAGTGGGCTGGTTTCAC; TCCCTTAATCACTTCTCCCAGCTCAGCAAAGATCTCGGCCCCTGACAGCAGGACATCTCC; AGACTCCTTCAGGGCAGCCTCCTGGGAATCCACGTACAGCACAGCTTCTTTCATGAGCTC; ATCATCCAGTTCTCTCCAGTCAGGTCTGCTGGCTCCAACAGCATTGATGTGAGCCCCTGG; CTTCACCCATTCACCAAACAAAATGGGCTCTGTTGCCAGGGTGACTGTGATGATCACATC; TGCACCTGCCACAGCCTCCTGGACCGAAGACAGACCCGTACCTCTCCTTGCACTGTGTCT; GCAAACTTCTCTGCATTTTCTTTGGTGCCGGTTCATATCCTCACCTCCTTAAGGAAAACT; GCTCTGGAAAACCTCATATGGCTGGGAGCCCGGACCCCACCCCAAGATGACAGCACTTCA; CTGCTGGGAGGTTCAAACCTGGGGGCATGGCAAAAACTGCACTGTCCTCTTGCATTAATA; CATTTCTCCTTGACGCCACAGGGGGCCTGGCTGGGCTCAAAGTACCCATACCCGGGGGAA; GGCAACCCGGTGATCCGGGTCCCTA;
• Restriction Sites: NotI-NotI
• ACCN: NM_001888
• Insert Size: 1320 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001888.2, NP_001879.1
• RefSeq Size: 1303 bp
• RefSeq ORF: 945 bp
• Locus ID: 1428
• UniProt ID: Q14894
• Cytogenetics: 16p12.2
• Domains: ODC_Mu_crystall
• Summary: Crystallins are separated into two classes: taxon-specific and ubiquitous. The former class is also called phylogenetically-restricted crystallins. The latter class constitutes the major proteins of vertebrate eye lens and maintains the transparency and refractive index of the lens. This gene encodes a taxon-specific crystallin protein that binds NADPH and has sequence similarity to bacterial ornithine cyclodeaminases. The encoded protein does not perform a structural role in lens tissue, and instead it binds thyroid hormone for possible regulatory or developmental roles. Mutations in this gene have been associated with autosomal dominant non-syndromic deafness. [provided by RefSeq, Sep 2014]