CRHBP (NM_001882) Human Untagged Clone

CAT#: SC118962

CRHBP (untagged)-Human corticotropin releasing hormone binding protein (CRHBP)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CRHBP
• Synonyms: CRF-BP; CRFBP
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118962 sequence for NM_001882 edited (data generated by NextGen Sequencing)
  ATGTCGCCCAACTTCAAACTTCAGTGTCACTTCATTCTCATCTTCCTGACGGCTCTAAGA
  GGGGAAAGCCGGTACCTAGAGCTGAGGGAAGCGGCGGACTACGATCCTTTCCTGCTCTTC
  AGCGCCAACCTGAAGCGGGAGCTGGCTGGGGAGCAGCCGTACCGCCGCGCTCTGCGGTGC
  CTGGACATGCTGAGCCTCCAGGGCCAGTTCACCTTCACCGCCGACCGGCCGCAGCTGCAC
  TGCGCAGCCTTCTTCATCAGCGAGCCCGAGGAGTTCATTACCATCCACTACGACCAGGTC
  TCCATCGACTGTCAGGGCGGCGACTTCCTGAAGGTATTTGATGGTTGGATTCTCAAGGGG
  GAGAAGTTCCCCAGTTCCCAGGATCATCCTCTCCCCTCAGCTGAGCGGTACATAGATTTC
  TGTGAGAGTGGTCTTAGCAGGAGGAGCATCAGATCTTCCCAGAATGTGGCCATGATCTTC
  TTCCGAGTCCATGAACCAGGAAATGGATTCACATTAACCATAAAGACAGACCCCAACCTC
  TTTCCTTGCAATGTCATTTCTCAGACTCCAAATGGAAAGTTTACCCTGGTAGTTCCACAC
  CAGCATCGAAACTGCAGCTTCTCCATAATTTATCCTGTGGTGATCAAAATATCTGATCTT
  ACCCTGGGACACGTAAATGGTCTTCAGTTAAAGAAATCCTCAGCAGGTTGCGAGGGAATA
  GGAGACTTTGTGGAGCTGCTGGGAGGAACTGGATTGGACCCTTCCAAGATGACGCCTTTA
  GCTGATCTCTGCTACCCCTTTCATGGCCCGGCCCAGATGAAAGTTGGCTGTGACAACACT
  GTGGTGCGCATGGTCTCCAGTGGAAAACACGTAAATCGTGTGACTTTTGAGTATCGTCAG
  CTGGAGCCGTACGAGCTGGAAAACCCAAATGGAAACAGTATCGGGGAATTCTGTTTGTCT
  GGTCTTTGA
  
  Clone variation with respect to NM_001882.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001882 unedited
  ACCCGATTTGTAACCGNACTTCATAGTAGGCGGCCAGCACATTCGGCACGAGGGTCTGCG
  CGATGGTGTAGGAAGGAAAGCCCAGGACCTCCGGAGCAGAGCACAGCAGCTGCAGAGGCA
  AGGCCAGCATGTCGCCCAACTTCAAACTTCAGTGTCACTTCATTCTCATCTTCCTGACGG
  CTCTAAGAGGGGAAAGCCGGTACCTAGAGCTGATTTTTCGGCGGACTACGATCCTTTCCT
  GCTCTTCAGCGCCAACCTGAAGCGGGAGCTGGCTGGGGAGCAGCCGTACCGCCGCGCTCT
  GCGGTGCCTGGACATGCTGAGCCTCCAGGGCCAGTTCACCTTCACCGCCGACCGGCCGCA
  GCTGCACTGCGCAGCCTTCTTCATCAGCGAGCCCGAGGAGTTCATTACCATCCACTACGA
  CCAGGTCTCCATCGACTGTCAGGGCGGCGACTTCCTGAAGGTATTTGATGGTTGGATTCT
  CAAGGGGGAGAAGTTCCCCAGTTCCCAGGATCATCCTCTCCCCTCAGCTGAGCGGTACAT
  AGATTTCTGTGAGAGTGGTCTTAGCAGGAGGAGCATCAGATCTTCCCAGAATGTGGCCAT
  GATCTTCTTCCGAGTCCATGAACCAGGAAATGGATTCACATTAACCATAAAGACAGACCC
  CAACCTCTTTTCTTGNCATGTCATTTCTCAGACTCCAATGGAAAGTTTACCCTGGTAGTT
  CCACACCAGCATCGAAACTGCAGCTTCTCCATAATTTATCCTGTGGTGATCAAAATATCT
  GATCTTACCCTGGGACACGTAAATGGGTCTTCAGTTAAAAAAATCCCTCANCAAGGTGCG
  AGGGAATTAGAGAACTTTGTGAGCTGCTGGNNAGAACTGGATTGAACCTTCCAGAAGACG
  CCTTTGCTGATCTCTGCTACCCCTTCAT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001882 unedited
  GCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGCCACATTCTGAATGTATTTATTCAT
  GTTTGTATTTGTGGATGGAAAATTTTTTCCATGCTACAAAGTTTGGTTAAAAACAAGTTG
  TTACAAATCAATAAATAATTGCTATAACTATTACCTATTGTAACAATAATAAATAGAAAA
  TAAACAGATGCAACTACTTTGCTATACACAAGACTTTATCCTGTTATAAAACTAATATAA
  GTGCTGCCACTTTTCTTTCTTTGAGTATTTTCCCTTGAGATACGATTTTTGTATTGCACG
  TTGCTTTGTGCTTGTCTTACCAGTTTCAGGTTTGTAGCACATCAAAGAATTACAAACATT
  TCCATTTAGAACTATGATCCTACCAATCAGGGGTTATTTTCTGCCATGTGTTCATTTACA
  GATTACAAACATAAAAGAAGCAAAGTACAAAAATTAATGCGTGTGTGTATGTGTGTGTGT
  GTGTGCGCGCGTGCGCTCAAGGCTGGGAATACTGACTGGTATGAAAGGCTTTTAACTAGT
  TGTGCATCAAAATCATACACAATGGCCATTAAAAACTCACTTAGCTATCAGCATGTAAAT
  CACTGGGTTGGTTATTCAAAGACCAGACAAACAGAATTCCCCGATACTGTTTCCATTTGG
  GTTTTCCAGCTCGTACGGCTTCAGCTGACGATACTCAAAAGTCACACGATTTACGTGTTT
  TCCACTACANACATGCGCACCACAGTGTTGTCACAGCCAAACTTTCATCTGGCCCGGGCC
  ATGAAAAGGGGTANCAAAGATCAGCTAAAGGCGTCATCTTGGAAGGGNTCCATTCAGNTC
  CTTCCAGCAGCTCACCAAAGTTTCCTATCCCTCGCAACCTGCTGAGGATTCTTTACTGAG
  ACCATTTACGGTCCACGGTAGATCAAAATTTGATCACACAGAAAATTTGGGAAGCTGCGT
  TCTAGCTGTGTGGACTACAAGGAACACTCACTTGAGTTAGAAGACATGCAGTAAAGTGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001882
• Insert Size: 1650 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001882.3, NP_001873.2
• RefSeq Size: 1854 bp
• RefSeq ORF: 969 bp
• Locus ID: 1393
• UniProt ID: P24387
• Cytogenetics: 5q13.3
• Gene Summary: Corticotropin-releasing hormone is a potent stimulator of synthesis and secretion of preopiomelanocortin-derived peptides. Although CRH concentrations in the human peripheral circulation are normally low, they increase throughout pregnancy and fall rapidly after parturition. Maternal plasma CRH probably originates from the placenta. Human plasma contains a CRH-binding protein which inactivates CRH and which may prevent inappropriate pituitary-adrenal stimulation in pregnancy. [provided by RefSeq, Jul 2008]