Cathepsin D (CTSD) (NM_001909) Human Untagged Clone

CAT#: SC118936

CTSD (untagged)-Human cathepsin D (CTSD)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Cathepsin D
• Synonyms: CLN10; CPSD; HEL-S-130P
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001909 edited
  GAATTCGGCACGAGGCCGGCTATAAGCGCACGGCCTCGGCGACCCTCTCCGACCCGGCCG
  CCGCCGCCATGCAGCCCTCCAGCCTTCTGCCGCTCGCCCTCTGCCTGCTGGCTGCACCCG
  CCTCCGCGCTCGTCAGGATCCCGCTGCACAAGTTCACGTCCATCCGCCGGACCATGTCGG
  AGGTTGGGGGCTCTGTGGAGGACCTGATTGCCAAAGGCCCCGTCTCAAAGTACTCCCAGG
  CGGTGCCAGCCGTGACCGAGGGGCCCATTCCCGAGGTGCTCAAGAACTACATGGACGCCC
  AGTACTACGGGGAGATTGGCATCGGGACGCCCCCCCAGTGCTTCACAGTCGTCTTCGACA
  CGGGCTCCTCCAACCTGTGGGTCCCCTCCATCCACTGCAAACTGCTGGACATCGCTTGCT
  GGATCCACCACAAGTACAACAGCGACAAGTCCAGCACCTACGTGAAGAATGGTACCTCGT
  TTGACATCCACTATGGCTCGGGCAGCCTCTCCGGGTACCTGAGCCAGGACACTGTGTCGG
  TGCCCTGCCAGTCAGCGTCGTCAGCCTCTGCCCTGGGCGGTGTCAAAGTGGAGAGGCAGG
  TCTTTGGGGAGGCCACCAAGCAGCCAGGCATCACCTTCATCGCAGCCAAGTTCGATGGCA
  TCCTGGGCATGGCCTACCCCCGCATCTCCGTCAACAACGTGCTGCCCGTCTTCGACAACC
  TGATGCAGCAGAAGCTGGTGGACCAGAACATCTTCTCCTTCTACCTGAGCAGGGACCCAG
  ATGCGCAGCCTGGGGGTGAGCTGATGCTGGGTGGCACAGACTCCAAGTATTACAAGGGTT
  CTCTGTCCTACCTGAATGTCACCCGCAAGGCCTACTGGCAGGTCCACCTGGACCAGGTGG
  AGGTGGCCAGCGGGCTGACCCTGTGCAAGGAGGGCTGTGAGGCCATTGTGGACACAGGCA
  CTTCCCTCATGGTGGGCCCGGTGGATGAGGTGCGCGAGCTGCAGAAGGCCATCGGGGCCG
  TGCCGCTGATTCAGGGCGAGTACATGATCCCCTGTGAGAAGGTGTCCACCCTGCCCGCGA
  TCACACTGAAGCTGGGAGGCAAAGGCTACAAGCTGTCCCCAGAGGACTACACGCTCAAGG
  TGTCGCAGGCCGGGAAGACCCTCTGCCTGAGCGGCTTCATGGGCATGGACATCCCGCCAC
  CCAGCGGGCCACTCTGGATCCTGGGCGACGTCTTCATCGGCCGCTACTACACTGTGTTTG
  ACCGTGACAACAACAGGGTGGGCTTCGCCGAGGCTGCCCGCCTCTAGTTCCCAAGGCGTC
  CGCGCGCCAGCACAGAAACAGAGGAGAGTCCCAGAGCAGGAGGCCCCTGGCCCAGCGGCC
  CCTCCCACACACACCCACACACTCGCCCGCCCACTGTCCTGGGCGCCCTGGAAGCCGGCG
  GCCCAAGCCCGACTTGCTGTTTTGTTCTGTGGTTTTCCCCTCCCTGGGTTCAGAAATGCT
  GCCTGCCTGTCTGTCTCTCCATCTGTTTGGTGGGGGTAGAGCTGATCCAGAGCACAGATC
  TGTTTCGTGCATTGGAAGACCCCACCCAAGCTTGGCAGCCGAGCTCGTGTATCCTGGGGC
  TCCCTTCATCTCCAGGGAGTCCCCTCCCCGGCCCTACCAGCGCCCGCTGGGCTGAGCCCC
  TACCCCACACCAGGCCGTCCTCCCGGGCCCTCCCTTGGAAACCTGCCCTGCCTGAGGGCC
  CCTCTGCCCAGCTTGGGCCCAGCTGGGCTCTGCCACCCTACCTGTTCAGTGTCCCGGGCC
  CGTTGAGGATGAGGCCGCTAGAGGCCTGAGGATGAGCTGGAAGGAGTGAGAGGGGACAAA
  ACCCACCTTGTTGGAGCCTGCAGGGTGGTGCTGGGACTGAGCCAGTCCCAGGGGCATGTA
  TTGGCCTGGAGGTGGGGTTGGGATTGGGGGCTGGTGCCAGCCTTCCTCTGCAGCTGACCT
  CTGTTGTCCTCCCCTTGGGCGGCTGAGAGCCCCAGCTGACATGGAAATACAGTTGTTGGC
  CTCCGGCCTCCCCTCAAAAAAAAAAAAAAAAAACTCGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001909 unedited
  NGGTTCAAATTTGTATACGACTCCTATAGGCGGCCCGCGAATTCGCACGAGGCCGGCTAT
  AAGCGCACGGCCTCGGCGACCCTCTCCGACCCGGCCGCCGCCGCCATGCAGCCCTCCAGC
  CTTCTGCCGCTCGCCCTCTGCCTGCTGGCTGCACCCGCCTCCGCGCTCGTCAGGATCCCG
  CTGCACAAGTTCACGTCCATCCGCCGGACCATGTCGGAGGTTGGGGGCTCTGTGGAGGAC
  CTGATTGCCAAAGGCCCCGTCTCAAAGTACTCCCAGGCGGTGCCAGCCGTGACCGAGGGG
  CCCATTCCCGAGGTGCTCAAGAACTACATGGACGCCCAGTACTACGGGGAGATTGGCATC
  GGGACGCCCCCCCAGTGCTTCACAGTCGTCTTCGACACGGGCTCCTCCAACCTGTGGGTC
  CCCTCCATCCACTGCAAACTGCTGGACATCGCTTGCTGGATCCACCACAAGTACAACAGC
  GACAAGTCCAGCACCTACGTGAAGAATGGTACCTCGTTTGACATCCACTATGGCTCGGGC
  AGCCTCTCCGGGTACCTGAGCCAGGACACTGTGTCGGTGCCCTGCCAGTCAGCGTCGTCA
  GCCTCTGCCCTGNGCGGTGTCAAAGTGGAGAGGCAGGTCTTTGGGGAGGCCACCAAGCAG
  CCAGGCATCACCTTCATCGCAGCCAAGTTCGATGGCATCCTGGGCATGGCCTACCCCCGC
  ATCTCCGTCAACAACGTGCTGCCCGTCTTCGACAACCTGATGCAGCAGAAGCTGGTGGAC
  CAGAACATCTTCTCCTTCTACCTGAGCAGGNACCCAGATGCGCAGCCTGGNGGGTGAGCT
  GATGCCTGGTGGCACAGACTCCAAGATTACAGGGTTTCTCTGTCTACCTGAATGTCACCC
  CGCAGGCTACTGGC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001909 unedited
  ACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGAGGGGAGGCCGNGAGC
  CAACAACTGTATTTCCATGTCAGCTGGGGCTCTCAGCCGCCCAAGGGGAGGACAACAGAG
  GTCAGCTGCAGAGGAAGGCTGGCACCAGCCCCCAATCCCAACCCCACCTCCAGGCCAATA
  CATGCCCCTGGGACTGGCTCAGTCCCAGCACCACCCTGCAGGCTCCAACAAGGTGGGTTT
  TGTCCCCTCTCACTCCTTCCAGCTCATCCTCAGGCCTCTAGCGGCCTCATCCTCAACGGG
  CCCGGGACACTGAACAGGTAGGGTGGCAGAGCCCAGCTGGGCCCAAGCTGGGCAGAGGGG
  CCCTCAGGCAGGGCAGGTTTCCAAGGGAGGGCCCGGGAGGACGGCCTGGTGTGGGGTAGG
  GGCTCAGCCCAGCGGGCGCTGGTAGGGCCGGGGAGGGGACTCCCTGGAGATGAAGGGAGC
  CCCAGGATACACGAGCTCGGCTGCCAAGCTTGGGTGGGGTCTTCCAATGCACGAAACAGA
  TCTGTGCTCTGGATCAGCTCTACCCCCACCAAACAGATGGAGAGACAGACAGGCAGGCAG
  CATTTCTGAACCCAGGGAGGGGAAAACCACAGAACANAACAGCAAGTCGGGCTTGGGCCG
  CCGGCTTCCAGGGCGCCCANNACAGTGGGCGGGCAANTGTGTGGGTGTGTGTGGGAGGGG
  CCCCCTGGCCAGGGGCCTCCTGCTCTGGGACTCTNCTCTGTTTCTGTGCTGGCGCGCGGA
  CGCCTTGGGACTAGAGGGCGGCANCTCCGCGAAGCCCACCTGTTGGTGTACGGGTCAACA
  CGTGTATTAGCGGCGATGAAAACCTCCCCCAGATCCAAATGGCCCCCGGGTTGCGGGATG
  TCATGCCATGAACCGCTCAGCAAAGGTCTTCCCN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001909
• Insert Size: 2190 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001909.3, NP_001900.1
• RefSeq Size: 2205 bp
• RefSeq ORF: 1239 bp
• Locus ID: 1509
• UniProt ID: P07339
• Cytogenetics: 11p15.5
• Domains: asp
• Protein Families: Druggable Genome, Protease
• Protein Pathways: Lysosome
• Gene Summary: This gene encodes a member of the A1 family of peptidases. The encoded preproprotein is proteolytically processed to generate multiple protein products. These products include the cathepsin D light and heavy chains, which heterodimerize to form the mature enzyme. This enzyme exhibits pepsin-like activity and plays a role in protein turnover and in the proteolytic activation of hormones and growth factors. Mutations in this gene play a causal role in neuronal ceroid lipofuscinosis-10 and may be involved in the pathogenesis of several other diseases, including breast cancer and possibly Alzheimer's disease. [provided by RefSeq, Nov 2015]