EIF5A (NM_001970) Human Untagged Clone

CAT#: SC118910

EIF5A (untagged)-Human eukaryotic translation initiation factor 5A (EIF5A), transcript variant B

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: EIF5A
• Synonyms: eIF-4D; EIF-5A; EIF5A1; eIF5AI
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118910 sequence for NM_001970 edited (data generated by NextGen Sequencing)
  ATGGCAGATGACTTGGACTTCGAGACAGGAGATGCAGGGGCCTCAGCCACCTTCCCAATG
  CAGTGCTCAGCATTACGTAAGAATGGCTTTGTGGTGCTCAAAGGCCGGCCATGTAAGATC
  GTCGAGATGTCTACTTCGAAGACTGGCAAGCACGGCCACGCCAAGGTCCATCTGGTTGGT
  ATTGACATCTTTACTGGGAAGAAATATGAAGATATCTGCCCGTCAACTCATAATATGGAT
  GTCCCCAACATCAAAAGGAATGACTTCCAGCTGATTGGCATCCAGGATGGGTACCTATCA
  CTGCTCCAGGACAGCGGGGAGGTACGAGAGGACCTTCGTCTCCCTGAGGGAGACCTTGGC
  AAGGAGATTGAGCAGAAGTACGACTGTGGAGAAGAGATCCTGATCACGGTGCTGTCTGCC
  ATGACAGAGGAGGCAGCTGTTGCAATCAAGGCCATGGCAAAATAA
  
  Clone variation with respect to NM_001970.4
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001970 unedited
  GGTGTACGAATTTTGTATACGACTCCTATAGGGCGGCCGCGATTCGGCACGAGGGCGGTA
  GAGGCGGCGGCGGCGGCGGCAGCGGGCTCGGAGGCAGCGGTTGGGCTCGCGGCGAGCGGA
  CGGGGTCGAGTCAGTGCGTTCGCGCGAGTTGGAATCGAAGCCTCTTAAAATGGCAGATGA
  CTTGGACTTCGAGACAGGAGATGCAGGGGCCTCAGCCACCTTCCCAATGCAGTGCTCAGC
  ATTACGTAAGAATGGCTTTGTGGTGCTCAAAGGCCGGCCATGTAAGATCGTCGAGATGTC
  TACTTCGAAGACTGGCAAGCACGGCCACGCCAAGGTCCATCTGGTTGGTATTGACATCTT
  TACTGGGAAGAAATATGAAGATATCTGCCCGTCAACTCATAATATGGATGTCCCCAACAT
  CAAAAGGAATGACTTCCAGCTGATTGGCATCCAGGATGGGTACCTATCACTGCTCCAGGA
  CAGCGGGGAGGTACGAGAGGACCTTCGTCTCCCTGAGGGAGACCTTGGCAAGGAGATTGA
  GCAGAAGTACGACTGTGGAGAAGAGATCCTGATCACGGTGCTGTCTGCCATGACAGAGGA
  GGCAGCTGTTGCAATCAAGGCCATGGCAAAATAACTGGCTCCCAGGGTGGCGGTGGTGGC
  AGCAGTGATCCTCTGAACCTGCAGAGGCCCCCTCCCCGAGCCTGGCCTGGCTCTGGCCCG
  GTCCTAAGCTGGACTCCTCCTACACAATTTATTTGACGTTNTATTTTGGTTTTCCCCACC
  CCCTCATCTGTCGNGGAGCCCCTGCCCTCCACCTAGCTCCTTGGCCAGNAGCGAGCGAAG
  CTGTGCCCTTGGTGAAGCTGCCCTCCTCTTCTCCCTNACACTAAGCCCTGGTGGGAAAGG
  GGGGNGGGGGCNCCCCCCNC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001970 unedited
  AGCTTGTNGTTCTATTCATGNTTGATTTTAACAAATGGTGTCGGGGNANANAGCCCGCAG
  GGAAGGGTAAAGCCCATGGGGGCAGGGCCCTCCCAGATGCCTGAGGAGGGGGCAGGTCCC
  CTCCCCTCTCCTCCTCTTCCCTCCCCATCTAAAGGGGTTTGGGGAGAGACACAGGCAGGC
  GAGGGGGCTGGTCCCCAGTCTGTTGGGGTGGTGCTTATGGTAAAGGGCTATGGGCAACAG
  GGGACCAGACCAGGGATGAGTGGGGAGGGCACAAGGACCATTTGCCAGAATCCACCGCTT
  TCTGATTCCAGATTGAATTAAAAAAAAAAAAAAAAAAAAAAATTGACTAACCCACAAGCA
  GCACCCACCCCCTTCTCCCCCACCAGGGCTGTACTGTGAGGGGAGAAGAGGAGGGCAGCT
  TCACCAAGGCCACAGCTTCGCTCGCTTCTGGCCAAGGGAGCTANGTGAAGGGCCAGGGCT
  CCCCCACAGATTGACGGGGTGGGGAAAACCAAACTCAACTTTCNATAAATTGTGNTNGAG
  GAGTCCAGTTTATGACCGCGCCATACCTCAGCCACAGCTCGGGAAGGGGGCCTCTCCTGC
  TTCAAACGGTCACTGNTGCCAACTCCACCACTCTAGCTCCAGTTTTTTTCGCTAGCCCTC
  CCCGCACCCCTACCCTTTCCGCAGTGGAACACCTATATTTAACCTGTCCCTTCTCAATCG
  CCTCTCTAGACTCAATTGCTTTCCCAAGTTCCCCTCACGGTAATCAAGTTCCTCCCCCCC
  TCTCTTTCTTTCCACAAAGTCCGTCCCATCTTCTCTTCCACCCCTTTTCATAATCNCCTC
  CCCATGTCCCNACCCCCGACNTTCTCCACTACCCTCTTTCCCACATTTCGTTAATCTCCC
  CTTTAACGCCCCTTCTTCTCCTCCAAACTTTTTCCCTACCNTAACATCTTGTATCCN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001970
• Insert Size: 6680 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001970.3, NP_001961.1
• RefSeq Size: 1290 bp
• RefSeq ORF: 465 bp
• Locus ID: 1984
• UniProt ID: P63241
• Cytogenetics: 17p13.1
• Domains: eIF-5a, KOW
• Gene Summary: mRNA-binding protein involved in translation elongation. Has an important function at the level of mRNA turnover, probably acting downstream of decapping. Involved in actin dynamics and cell cycle progression, mRNA decay and probably in a pathway involved in stress response and maintenance of cell wall integrity. With syntenin SDCBP, functions as a regulator of p53/TP53 and p53/TP53-dependent apoptosis. Regulates also TNF-alpha-mediated apoptosis. Mediates effects of polyamines on neuronal process extension and survival. May play an important role in brain development and function, and in skeletal muscle stem cell differentiation. Also described as a cellular cofactor of human T-cell leukemia virus type I (HTLV-1) Rex protein and of human immunodeficiency virus type 1 (HIV-1) Rev protein, essential for mRNA export of retroviral transcripts.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (B) contains an alternate in-frame exon in the 5' coding region and uses a downstream start codon compared to variant A. The encoded isoform (B) has a shorter N-terminus compared to isoform A. Variants B, C, and D encode the same isoform.