EEF1A2 (NM_001958) Human Untagged Clone

CAT#: SC118902

EEF1A2 (untagged)-Human eukaryotic translation elongation factor 1 alpha 2 (EEF1A2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: EEF1A2
• Synonyms: DEE33; EEF1AL; EF-1-alpha-2; EF1A; EIEE33; HS1; MRD38; STN; STNL
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118902 sequence for NM_001958 edited (data generated by NextGen Sequencing)
  ATGGGCAAGGAGAAGACCCACATCAACATCGTGGTCATCGGCCACGTGGACTCCGGAAAG
  TCCACCACCACGGGCCACCTCATCTACAAATGCGGAGGTATTGACAAAAGGACCATTGAG
  AAGTTCGAGAAGGAGGCGGCTGAGATGGGGAAGGGATCCTTCAAGTATGCCTGGGTGCTG
  GACAAGCTGAAGGCGGAGCGTGAGCGCGGCATCACCATCGACATCTCCCTCTGGAAGTTC
  GAGACCACCAAGTACTACATCACCATCATCGATGCCCCCGGCCACCGCGACTTCATCAAG
  AACATGATCACGGGTACATCCCAGGCGGACTGCGCAGTGCTGATCGTGGCGGCGGGCGTG
  GGCGAGTTCGAGGCGGGCATCTCCAAGAATGGGCAGACGCGGGAGCATGCCCTGCTGGCC
  TACACGCTGGGTGTGAAGCAGCTCATCGTGGGCGTGAACAAAATGGACTCCACAGAGCCG
  GCCTACAGCGAGAAGCGCTACGACGAGATCGTCAAGGAAGTCAGCGCCTACATCAAGAAG
  ATCGGCTACAACCCGGCCACCGTGCCCTTTGTGCCCATCTCCGGCTGGCACGGTGACAAC
  ATGCTGGAGCCCTCCCCCAACATGCCGTGGTTCAAGGGCTGGAAGGTGGAGCGTAAGGAG
  GGCAACGCAAGCGGCGTGTCCCTGCTGGAGGCCCTGGACACCATCCTGCCCCCCACGCGC
  CCCACGGACAAGCCCCTGCGCCTGCCGCTGCAGGACGTGTACAAGATTGGCGGCATTGGC
  ACGGTGCCCGTGGGCCGGGTGGAGACCGGCATCCTGCGGCCGGGCATGGTGGTGACCTTT
  GCGCCAGTGAACATCACCACTGAGGTGAAGTCAGTGGAGATGCACCACGAGGCTCTGAGC
  GAAGCTCTGCCCGGCGACAACGTCGGCTTCAATGTGAAGAACGTGTCGGTGAAGGACATC
  CGGCGGGGCAACGTGTGTGGGGACAGCAAGTCTGACCCGCCGCAGGAGGCTGCTCAGTTC
  ACCTCCCAGGTCATCATCCTGAACCACCCGGGGCAGATTAGCGCCGGCTACTCCCCGGTC
  ATCGACTGCCACACAGCCCACATCGCCTGCAAGTTTGCGGAGCTGAAGGAGAAGATTGAC
  CGGCGCTCTGGCAAGAAGCTGGAGGACAACCCCAAGTCCCTGAAGTCTGGAGACGCGGCC
  ATCGTGGAGATGGTGCCGGGAAAGCCCATGTGTGTGGAGAGCTTCTCCCAGTACCCGCCT
  CTCGGCCGCTTCGCCGTGCGCGACATGAGGCAGACGGTGGCCGTAGGCGTCATCAAGAAC
  GTGGAGAAGAAGAGCGGCGGCGCCGGCAAGGTCACCAAGTCGGCGCAGAAGGCGCAGAAG
  GCGGGCAAGTGA
  
  Clone variation with respect to NM_001958.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001958 unedited
  ACCTGGATTTGTAAACCGNACTTCACTATAGGGCGGCACAAGCAATTCGCACGAGGGAGA
  CCTCGGCTCCGGNAATCACTGCAGCCCCCCTCGCCCTGAGCCAGAGCACCCCGGGTCCCG
  CCAGCCCCTCACACTCCCAGCAAAATGGGCAAGGAGAAGACCCACATCAACATCGTGGTC
  ATCGGCCACGTGGACTCCGGAAAGTCCACCACCACGGTTTTCCTCATCTACAAATGCGGA
  GGTATTGACAAAAGGACCATTGAGAAGTTCGAGAAGGAGGCGGCTGAGATGGGGAAGGGA
  TCCTTCAAGTATGCCTGGGTGCTGGACAAGCTGAAGGCGGAGCGTGAGCGCGGCATCACC
  ATCGACATCTCCCTCTGGAAGTTCGAGACCACCAAGTACTACATCACCATCATCGATGCC
  CCCGGCCACCGCGACTTCATCAAGAACATGATCACGGGTACATCCCAGGCGGACTGCGCA
  GTGCTGATCGTGGCGGCGGGCGTGGGCGAGTTCGAGGCGGGCATCTCCAAGAATGGGCAG
  ACGCGGGAGCATGCCCTGCTGGCCTACACGCTGGGTGTGAAGCAGCTCATCGTGGGCGTG
  AACAAAATGGACTCCACAGAGCCGGCCTACAGCGAGAAGCGCTACGACGAGATCGTCAAG
  GAAGTCAGCGCCTACATCAAGAAGATCGGCTACAACCCGGCCACCGTGCCCTTTGTGCCC
  CTCTCCGGCTGGCACGGTGACAACATGCTGGAGCCCTCCCCCACATGCCGTGGTTCAAGG
  GCTGGAAGGTGGAGCGTAAGGAGGGCAACGCAANGCGCGTGTCCCTGCTGGNAGCCCTGG
  ACACATNCTGCCCCCCACGCGCCCACGGACAGCCCCTGCGCCTGCCGCTGCAAAACGTGT
  ACAAAATTGGCGGCATTGGCACCGTG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001958 unedited
  GGCGGTCACAGTGGAGCCACGCCGTCTCTGTTCAGGAAACAGCTATGACCGCGGCCGCAA
  TCTAGAGTCGAGTTTTTTTTTTTTTTTTTTAGGGTCTCTCACTTTATTGGGAAAACACAC
  ACTGATCGTGGCGAGCCCTGGCCCCGGAACCCTGGGGGGGGCGCGCCTTTCCCCGTGGAC
  AACCTCCACTGTCCCATTGCGGCGCAGCCGGGGATCGGTGAGCCGTGGCCTCTGGCGCTC
  CACTGGCCCGACTTCCGCGCCTTTTGCGTCGCACTTGCTGACCTTGCCGGCGCCGCCGCT
  CTTCTTCTCCACGTTCTTGATGACGCCTACGGCCACCGTCTGCCTTATGCTCCGCACTGT
  TAATCGGCCGATAAGCGGGTACTGGGAGAAGCTCCTCCCACACATGGTCCTTTCCCGGCA
  CCATCTCCACGATGGCCGTGTCTCCAGACTTCAGGGACTTGGTGTTGCCCCCCCTCTTCT
  TGTCCGAGCGCCGTGTAATCTTCTCCTTCTCCTCCGCCAACTTGCATGCCACTTTGTTCG
  GCGTGGTAGTCGATGACCGGGGAGTAGCCGGCGCCACCCTTGTCCCGGGCGGGTCAAGGA
  TGATGGACCTGNGAGGTGAACCGAATCATCCTCCTGTTTCTGGGCACAACTTGCCGGGCT
  CCTTTTACTTTGGCCCCGCCGGGGCGTCCCTCACCCGAACCGGTTCTTTATATTTGAAAC
  CCACCCTTTTNTTCTTGTAAAAGCTTCGCTTCAAAGCCCCCGGGTGTCTCGGCCCGGGCT
  AGCCCCGTGATGGTGTAGTTCTATTGCGCCCTTAGGCCCCCNCCCGCCCCCGGGCCTTTA
  GATGCCCGTTCNTCCACTCGGCGCCTGTGTGCCCTGGCCCATACCACCCCACTCTTTTTA
  AAGATACTGGAAAGAGAAGAGAGAG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001958
• Insert Size: 1890 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001958.2, NP_001949.1
• RefSeq Size: 1841 bp
• RefSeq ORF: 1392 bp
• Locus ID: 1917
• UniProt ID: Q05639
• Cytogenetics: 20q13.33
• Domains: GTP_EFTU, GTP_EFTU_D3, GTP_EFTU_D2
• Protein Families: Druggable Genome
• Gene Summary: This gene encodes an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 2) is expressed in brain, heart and skeletal muscle, and the other isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas. This gene may be critical in the development of ovarian cancer. [provided by RefSeq, Mar 2014]