HARS1 (NM_002109) Human Untagged Clone

CAT#: SC118837

HARS (untagged)-Human histidyl-tRNA synthetase (HARS)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: HARS1
• Synonyms: CMT2W; HARS; HRS; USH3B
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118837 sequence for NM_002109 edited (data generated by NextGen Sequencing)
  ATGGCAGAGCGTGCGGCGCTGGAGGAGCTGGTGAAACTTCAGGGAGAGCGCGTGCGAGGC
  CTCAAGCAGCAGAAGGCCAGCGCCGAGCTGATCGAGGAGGAGGTGGCGAAACTCCTGAAA
  CTGAAGGCACAGCTGGGTCCTGATGAAAGCAAACAGAAATTTGTGCTCAAAACCCCCAAG
  GGCACAAGAGACTATAGTCCCCGGCAGATGGCAGTTCGCGAGAAGGTGTTTGACGTAATC
  ATCCGTTGCTTCAAGCGCCACGGTGCAGAAGTCATTGATACACCTGTATTTGAACTAAAG
  GAAACACTGATGGGAAAGTATGGGGAAGACTCCAAGCTTATCTATGACCTGAAGGACCAG
  GGCGGGGAGCTCCTGTCCCTTCGCTATGACCTCACTGTTCCTTTTGCTCGGTATTTGGCA
  ATGAATAAACTGACCAACATTAAACGCTACCACATAGCAAAGGTATATCGGCGGGATAAC
  CCAGCCATGACCCGTGGCCGATACCGGGAATTCTACCAGTGTGATTTTGACATTGCTGGG
  AACTTTGATCCCATGATCCCTGATGCAGAGTGCCTGAAGATCATGTGCGAGATCCTGAGT
  TCACTTCAGATAGGCGACTTCCTGGTCAAGGTAAACGATCGACGCATTCTAGATGGGATG
  TTTGCTATCTGTGGTGTTTCTGACAGCAAGTTCCGTACCATCTGCTCCTCAGTAGACAAG
  CTGGACAAGGTGTCCTGGGAAGAGGTGAAGAATGAGATGGTGGGAGAGAAGGGCCTTGCA
  CCTGAGGTGGCTGACCGCATTGGGGACTATGTCCAGCAACATGGTGGGGTATCCCTGGTG
  GAACAGCTGCTCCAGGATCCTAAACTATCCCAAAACAAGCAGGCCTTGGAGGGCCTGGGA
  GACCTGAAGTTGCTCTTTGAGTACCTGACCCTATTTGGCATTGATGACAAAATCTCCTTT
  GACCTGAGCCTTGCTCGAGGGCTGGATTACTACACTGGGGTGATCTATGAGGCAGTGCTG
  CTACAGACCCCAGCCCAGGCAGGGGAAGAGCCCCTGGGTGTGGGCAGTGTGGCTGCTGGA
  GGACGCTATGATGGGCTAGTGGGCATGTTCGACCCCAAAGGGCGCAAGGTGCCATGTGTG
  GGGCTCAGCATTGGGGTGGAGCGGATTTTCTCCATCGTGGAACAGAGACTAGAGGCTTTG
  GAGGAGAAGATACGGACCACGGAGACACAGGTGCTTGTGGCATCTGCACAGAAGAAGCTG
  CTAGAGGAAAGACTAAAGCTTGTCTCAGAACTGTGGGATGCTGGGATCAAGGCTGAGCTG
  CTGTACAAGAAGAACCCAAAGCTACTGAACCAGTTACAGTACTGTGAGGAGGCAGGCATC
  CCACTGGTGGCTATCATCGGCGAGCAGGAACTCAAGGATGGGGTCATCAAGCTCCGTTCA
  GTGACGAGCAGGGAAGAGGTGGATGTCCGAAGAGAAGACCTTGTGGAGGAAATCAAAAGG
  AGAACAGGCCAGCCCCTCTGCATCTGCTGA
  
  Clone variation with respect to NM_002109.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002109 unedited
  ATACGACTTCACTATAGGGCGGCCGCGAATTCGCACGAGGGGCAACCACCGCAGGTCGAG
  ACAGCAGGCGGCTCAAGTGGACAGCCGGGATGGCAGAGCGTGCGGCGCTGGAGGAGCTGG
  TGAAACTTCAGGGAGAGCGCGTGCGAGGCCTCAAGCAGCAGAAGGCCAGCGCCGAGCTGA
  TCGAGGAGGAGGTGGCGAAACTCCTGAAACTGAAGGCACAGCTGGGTCCTGATGAAAGCA
  AACAGAAATTTGTGCTCAAAACCCCCAAGGGCACAAGAGACTATAGTCCCCGGCAGATGG
  CAGTTCGCGAGAAGGTGTTTGACGTAATCATCCGTTGCTTCAAGCGCCACGGTGCAGAAG
  TCATTGATACACCTGTATTTGAACTAAAGGAAACACTGATGGGAAAGTATGGGGAAGACT
  CCAAGCTTATCTATGACCTGAAGGACCAGGGCGGGGAGCTCCTGTCCCTTCGCTATGACC
  TCACTGTTCCTTTTGCTCGGTATTTGGCAATGAATAAACTGACCAACATTAAACGCTACC
  ACATAGCAAAGGTATATCGGCGGGATAACCCAGCCATGACCCGTGGCCGATACCGGGAAT
  TCTACCAGTGTGATTNTGACATTGCTGGGAACTTTGATCCCATGATCCCTGATGCAGAGT
  GCCTGAAGATCATGTGCGAGATCCTGAGNTCACTTCAGATAGGCGACTTCCTGGTCAGGT
  AAACGATCGACGCATTCTAGATGGGATGNTNGCTATCTGTGGTGTTTCTGACAGCAAGTT
  CCGTACCATCTGCTNCTCAGTANACAAGCTGGACAAGGTGTNCTGGNAAGAGGTGAAAAT
  GANATGGTGGNAGAGAAGGGCTTGCACCTGAGTGGCTGACCGATTGGGGACTATTGTCAG
  CACATGTTGGGGTTTCCCTGGTGAACANTGCTC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002109 unedited
  GCTGTGGACCCGCGGCCGCAATCTANGATCGAGTTTTTTTTTTTTTTTTTTCCGTGGTTG
  AGGCATTTTATTGGACCTTTGGCAATTGGTGGTGGGGAGGCATCTGCTCCAACTGGTGCG
  GGGCCCTGCAGATGGGACCATCTCAGGCTGGGTCCTTGTAGCCCAGGAGCACAGACTGGA
  CTAAGCCTCCTGGGCCTTGTATGAAAAAGGTGGTTGTNACCTGGCCGTTTTTGCCAGTAA
  TAATCAATAAAATAACCATAATAAAAATCAAAGGCTCTGTTCTGACCACTCTTCAGGTCT
  TCCGCTGAAACCGAAAAGTGCAAAGCAATTGAAGTACATATGCAGTTTGTCTTAACCTCA
  AATAGTGCCAGTCCCACTTCCTTTCCTCTGATAGTTTGTTCAGTTCAGCAGATGCAGAGG
  GGCTGGCCTGTTCTCCTTTTGATTTCCTCCACAAGGTCTTCTCTTCGGACATCCACCTCT
  TCCCTGCTCGTCACTGAACGGAGCTTGATGACCCCATCCTTGAGTTCCTGCTCGCCGATG
  ATAGCCACCAGTGGGATGCCTGCCTCCTCACAGTACTGTAACTGGTTCAGTAGCTTTGGG
  TTCTTCTTGTACAGCAGCTCAGCCTTGATCCCAGCATCCCACAGTTCTTGAGACAAGCTT
  TAGTCTTTCCTCTAGCAGCTTCTTCTGTGCAGATGCCACCAGCACCTGTGTCTCCGTGGT
  CCCTATCTTCTCCTCCAAAGCCTTTATTTCTTGTCCACGATGGAGAAATCCGCTCCACCC
  TATGCTGGACCCCAAACTGGCACCTTGCCCCCTTTGGGGTTAACATGCCACTTCCCCTTA
  TAAGGTTCTCCAGAAGCCAATTGTCCCCACCCAGGGTTTTTCCCTGCCGGGCTGGGGTCT
  GACCACCCTGCCTCTTTATAACCCCATGTAATATCCACCCTTACCAGGTTCAGGTAAGGA
  ATTTTGTTTAATGCCAAATGGTCAG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002109
• Insert Size: 2050 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002109.3, NP_002100.2
• RefSeq Size: 1981 bp
• RefSeq ORF: 1530 bp
• Locus ID: 3035
• UniProt ID: P12081
• Cytogenetics: 5q31.3
• Domains: WHEP-TRS, tRNA-synt_2b, HGTP_anticodon
• Protein Pathways: Aminoacyl-tRNA biosynthesis
• Gene Summary: Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. The protein encoded by this gene is a cytoplasmic enzyme which belongs to the class II family of aminoacyl-tRNA synthetases. The enzyme is responsible for the synthesis of histidyl-transfer RNA, which is essential for the incorporation of histidine into proteins. The gene is located in a head-to-head orientation with HARSL on chromosome five, where the homologous genes share a bidirectional promoter. The gene product is a frequent target of autoantibodies in the human autoimmune disease polymyositis/dermatomyositis. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).