H3.3A (H3F3A) (NM_002107) Human Untagged Clone

CAT#: SC118836

H3F3A (untagged)-Human H3 histone, family 3A (H3F3A)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: H3.3A
• Synonyms: H3-3B; H3.3A; H3F3; H3F3A
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118836 sequence for NM_002107 edited (data generated by NextGen Sequencing)
  ATGGCTCGTACAAAGCAGACTGCCCGCAAATCGACCGGTGGTAAAGCACCCAGGAAGCAA
  CTGGCTACAAAAGCCGCTCGCAAGAGTGCGCCCTCTACTGGAGGGGTGAAGAAACCTCAT
  CGTTACAGGCCTGGTACTGTGGCGCTCCGTGAAATTAGACGTTATCAGAAGTCCACTGAA
  CTTCTGATTCGCAAACTTCCCTTCCAGCGTCTGGTGCGAGAAATTGCTCAGGACTTTAAA
  ACAGATCTGCGCTTCCAGAGCGCAGCTATCGGTGCTTTGCAGGAGGCAAGTGAGGCCTAT
  CTGGTTGGCCTTTTTGAAGACACCAACCTGTGTGCTATCCATGCCAAACGTGTAACAATT
  ATGCCAAAAGACATCCAGCTAGCACGCCGCATACGTGGAGAACGTGCTTAA
  
  Clone variation with respect to NM_002107.4
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002107 unedited
  GCCGCGAATTCGGCACGAGCCGCCTCTCGCTCGCCGAGCTCCAGCCGAAGGAGAAGGGGG
  GTAAGTAAGGAGGTCTCTGTACCATGGCTCGTACAAAGCAGACTGCCCGCAAATCGACCG
  GTGGTAAAGCACCCAGGAAGCAACTGGCTACAAAAGCCGCTCGCAAGAGTGCGCCCTCTA
  CTGGAGGGGTGAAGAAACCTCATCGTTACAGGCCTGGTACTGTGGCGCTCCGTGAAATTA
  GACGTTATCAGAAGTCCACTGAACTTCTGATTCGCAAACTTCCCTTCCAGCGTCTGGTGC
  GAGAAATTGCTCAGGACTTTAAAACAGATCTGCGCTTCCAGAGCGCAGCTATCGGTGCTT
  TGCAGGAGGCAAGTGAGGCCTATCTGGTTGGCCTTTTTGAAGACACCAACCTGTGTGCTA
  TCCATGCCAAACGTGTAACAATTATGCCAAAAGACATCCAGCTAGCACGCCGCATACGTG
  GAGAACGTGCTTAAGAATCCACTATGATGGGAAACATTTCATTCTCAAAAAAAAAAAAAA
  AAAANCTCGACTCTAGAATTGCGGCCGCGGTCATAGCTTGTTTCCTGAACAGATCCCGGG
  TGGCATCCCTGTGACCCCTCCCCAGTGCCTCTCCTGGCCCTGGAAGTTGCCACTCCAGTG
  CCCACCAGCCTTGTCCTAATAAAATTAAAGTGCATCATTTTGTCTGACTAGGTGTCCTTC
  TATAATATATGGGGTGANGGGGGNGGGN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002107 unedited
  NCCCCCATTACTTTGNACCGTCGGCCGCATNCTACGATCGAGTTTTTTTTTTTTTTTTTT
  GAGAATGAAATGTTTCCCATCATAGTGGATTCTTAAGCACGTTCTCCACGTATGCGGCGT
  GCTAGCTGGATGTCTTTTGGCATAATTGTTACACGTTTGGCATGGATAGCACACAGGTTG
  GTGTCTTCAAAAAGGCCAACCAGATAGGCCTCACTTGCCTCCTGCAAAGCACCGATAGCT
  GCGCTCTGGAAGCGCAGATCTGTTTTAAAGTCCTGAGCAATTTCTCGCACCAGACGCTGG
  AAGGGAAGTTTGCGAATCAGAAGTTCAGTGGACTTCTGATAACGTCTAATTTCACGGAGC
  GCCACAGTACCAGGCCTGTAACGATGAGGTTTCTTCACCCCTCCAGTAGAGGGCGCACTC
  TTGCGAGCGGCTTTTGTAGCCAGTTGCTTCCTGGGTGCTTTACCACCGGTCGATTTGCGG
  GCAGTCTGCTTTGTACGAGCCATGGTACAGAGACCTCCTTACTTACCCCCCTTCTCCTTC
  GGCTGGAGCTCGGCGAGCGAGAGGCGGCTCGTGCCGAATTCGCGGCCGCCCTATAGTGAG
  CTCCATTACAAAATTCTGACGGCTCACTAAACGAACTCTGCTTATATAGACCTCTCCCCG
  TACACCCCTACCCTCCATTTGCGTAACCGGGCGGGGTTATTACTACCACTTTGCCAGTCC
  CTTTGATCTTCCTGCCAAACAAACTCCCTTTCACCTCCATGGTGCGTGAGACCTCGCAAA
  CCCCTTTATGCCACCCCCCTATCCCCCCCCCAGCTTACTGCCCACCCCCTTTCCCCACGT
  CCCCAGGCCCCTTTATACCCTATCATCTCCCCATTCCCCAACCCCCCTCGCCCCCCCTCT
  GCCCT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002107
• Insert Size: 530 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002107.3, NP_002098.1
• RefSeq Size: 1117 bp
• RefSeq ORF: 411 bp
• Locus ID: 3020
• UniProt ID: P84243
• Cytogenetics: 1q42.12
• Domains: H3, histone
• Protein Pathways: Systemic lupus erythematosus
• Gene Summary: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene contains introns and its mRNA is polyadenylated, unlike most histone genes. The protein encoded is a replication-independent member of the histone H3 family. [provided by RefSeq, Jul 2008]