HMG1 (HMGB1) (NM_002128) Human Untagged Clone

CAT#: SC118802

HMGB1 (untagged)-Human high mobility group box 1 (HMGB1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: HMG1
• Synonyms: HMG-1; HMG1; HMG3; SBP-1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_002128, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGGCAAAGGAGATCCTAAGAAGCCGAGAGGCAAAATGTCATCATATGCATTTTTTGTGCAAACTTGTC
  GGGAGGAGCATAAGAAGAAGCACCCAGATGCTTCAGTCAACTTCTCAGAGTTTTCTAAGAAGTGCTCAGA
  GAGGTGGAAGACCATGTCTGCTAAAGAGAAAGGAAAATTTGAAGATATGGCAAAAGCGGACAAGGCCCGT
  TATGAAAGAGAAATGAAAACCTATATCCCTCCCAAAGGGGAGACAAAAAAGAAGTTCAAGGATCCCAATG
  CACCCAAGAGGCCTCCTTCGGCCTTCTTCCTCTTCTGCTCTGAGTATCGCCCAAAAATCAAAGGAGAACA
  TCCTGGCCTGTCCATTGGTGATGTTGCGAAGAAACTGGGAGAGATGTGGAATAACACTGCTGCAGATGAC
  AAGCAGCCTTATGAAAAGAAGGCTGCGAAGCTGAAGGAAAAATACGAAAAGGATATTGCTGCATATCGAG
  CTAAAGGAAAGCCTGATGCAGCAAAAAAGGGAGTTGTCAAGGCTGAAAAAAGCAAGAAAAAGAAGGAAGA
  GGAGGAAGATGAGGAAGATGAAGAGGATGAGGAGGAGGAGGAAGATGAAGAAGATGAAGATGAAGAAGAA
  GATGATGATGATGAATAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002128 unedited
  ACTCACTATAGGCGGCCGCGAATTCGCACGAGGAGAGTGAGGAGGCTGCGTCTGGCTCCC
  GCTCTCACAGCCATTGCAGTACATTGAGCTCCATAGAGACAGCGCCGGGGCAAGTGAGAG
  CCGGACGGGCACTGGGCGACTCTGTGCCTCGCTGAGGAAAAATAACTAAACATGGGCAAA
  GGAGATCCTAAGAAGCCGAGAGGCAAAATGTCATCATATGCATTTTTTGTGCAAACTTGT
  CGGGAGGAGCATAAGAAGAAGCACCCAGATGCTTCAGTCAACTTCTCAGAGTTTTCTAAG
  AAGTGCTCAGAGAGGTGGAAGACCATGTCTGCTAAAGAGAAAGGAAAATTTGAAGATATG
  GCAAAAGCGGACAAGGCCCGTTATGAAAGAGAAATGAAAACCTATATCCCTCCCAAAGGG
  GAGACAAAAAAGAAGTTCAAGGATCCCAATGCACCCAAGAGGCCTCCTTCGGCCTTCTTC
  CTCTTCTGCTCTGAGTATCGCCCAAAAATAAAGGAGAACATCCTGGCCTGTCCATTGGTG
  ATGTTGCGAAGAAACTGGGAGAGATGTGGAATAACACTGCTGCAGATGACAAGCAGCCTT
  ATGAAAAGAAGGCTGCGAAGCTGAAGGAANAATACAAAAGGATATTGCTGCATATCGAGC
  TAAAGAAAGCCTGATGCAGCAAANAGGGGAGTGTCAAGGGCTGAAAAAGNCAGAAAAGAA
  GGAAGAAGANGAAGATGANGAANATGAANAGGATGANNGAGAGNANGAAGATGAANAANA
  TGAAGATGAAGAAAAGATGATGATGATGAAATAAGNTGGTTCTAAGCGCAGTTTTTTTTT
  TCTTGGCTATAAGCATTTTACCCCCCCTGTACACAACTNACTTCTTTTAAGAAAAAATTG
  AATGNANGGCTGGGTAANAATTGTTTTAAACTGNACAGGCCTTTTTTTGGATAGGTAACA
  CCTACCCAAGTGGGTTAAAAAACCTGGCCCGGGGGGATTTCAAAGCACCTACCTGCTGAC
  GGNGGGGGGA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002128 unedited
  NNNTTAGCTCTGNNACCGCGGCCGCATTCTAGGATCGAGNTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTTTTTTTTTTTAACTAAAAAAAAAAATGGATTTACTTAAAAGCATTCA
  AAATGGCAACAAAACAGCTGCAACTTTTTTTTTTTTGCAATTACAGAGGGGTATTCAGTT
  AACAAAACAACAATTATTTCGTATAAGCTGCATCAAAGACAACTGAAGATGAAAAAACTA
  CCATCCCCATATATAACTAATTTGGGCTGGGCCCCAACAAAAACCTGCTTTAAATTTCCA
  TGCCAATTTACAACCCCCATACTGTACCAGGCAAGGTTAGGGGCTATTGAAAATACCACC
  AGGACAGGGCTATCTAAAGACACATTCGGGAGGGGGTTAACTATACAAAAAAAGACACTG
  GACAGTTTAAAAACAAATTTTACACAGCCTTACATTTAAATTTTTTTCTTTAAAAGGAGT
  GAGTTGGGCACAGGGGGGTTAAATGCTTTATAGACAAAAAAAAAAAAACTGCGCTAGAAC
  CAACTTATTCATCATCATCATCTTCTTCTTAATCTTCATATTCTTAATCTTCCTCCTCCT
  CCCCATCCCCTTTATCTTCCTCATCTTCCTCCTCTTCCTTTCTTTTCTTGCTTTTTNCAG
  CCTTGACAACTCCCTTTTTTGCTGGATAAGGCCTTTCCTTTAACCCGAAAAGCCGAAATA
  ACCCTTTTGGAATTTTTCCTTTAGCTTCCCAACCTCTTTTTTATAAGGCGGCTTGCATTC
  GGCAGAGGGTTTTTCCCAATCTTCCCCGGTTTTTCCCACCACCACAAGGGCCAGCCCAGG
  AGGTCCCTTTTGTTTTTGGGCCAACCCCAAAACCAAAGAGAAAAAGCGCCCAAGAAGCCC
  TCTGGGGGC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002128
• Insert Size: 1320 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002128.3, NP_002119.1
• RefSeq Size: 3428 bp
• RefSeq ORF: 648 bp
• Locus ID: 3146
• UniProt ID: P09429
• Cytogenetics: 13q12.3
• Domains: HMG
• Protein Families: Druggable Genome, Stem cell - Pluripotency, Transcription Factors
• Protein Pathways: Base excision repair
• Gene Summary: This gene encodes a protein that belongs to the High Mobility Group-box superfamily. The encoded non-histone, nuclear DNA-binding protein regulates transcription, and is involved in organization of DNA. This protein plays a role in several cellular processes, including inflammation, cell differentiation and tumor cell migration. Multiple pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Sep 2015]
  Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1, 2 and 3 encode the same protein.