Kir4.1 (KCNJ10) (NM_002241) Human Untagged Clone

SKU SC118741

KCNJ10 (untagged)-Human potassium inwardly-rectifying channel, subfamily J, member 10 (KCNJ10)

Product Data

• Type: Human Untagged Clone
• Target Symbol: Kir4.1
• Synonyms: BIRK-10; KCNJ13-PEN; KIR1.2; KIR4.1; SESAME
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118741 sequence for NM_002241 edited (data generated by NextGen Sequencing); ATGACGTCAGTTGCCAAGGTGTATTACAGTCAGACCACTCAGACAGAAAGCCGGCCCCTA; ATGGGCCCAGGGATACGACGGCGGAGAGTCCTGACAAAAGATGGTCGCAGCAACGTGAGA; ATGGAGCACATTGCCGACAAGCGCTTCCTCTACCTCAAGGACCTGTGGACAACCTTCATT; GACATGCAGTGGCGCTACAAGCTTCTGCTCTTCTCTGCGACCTTTGCAGGCACATGGTTC; CTCTTTGGCGTGGTGTGGTATCTGGTAGCTGTGGCACATGGGGACCTGCTGGAGCTGGAC; CCCCCGGCCAACCACACCCCCTGTGTGGTACAGGTGCACACACTCACTGGAGCCTTCCTC; TTCTCCCTTGAATCCCAAACCACCATTGGCTATGGCTTCCGCTACATCAGTGAGGAATGT; CCACTGGCCATTGTGCTTCTTATTGCCCAGCTGGTGCTCACCACCATCCTGGAAATCTTC; ATCACAGGTACCTTCCTGGCGAAGATTGCCCGGCCCAAGAAGCGGGCTGAGACCATTCGT; TTCAGCCAGCATGCAGTTGTGGCCTCCCACAATGGCAAGCCCTGCCTCATGATCCGAGTT; GCCAATATGCGCAAAAGCCTCCTCATTGGCTGCCAGGTGACAGGAAAACTGCTTCAGACC; CACCAAACCAAGGAAGGGGAGAACATCCGGCTCAACCAGGTCAATGTGACTTTCCAAGTA; GACACAGCCTCTGACAGCCCCTTCCTTATTCTACCCCTTACCTTCTATCATGTGGTAGAT; GAGACCAGTCCCTTGAAAGATCTCCCTCTTCGCAGTGGTGAGGGTGACTTTGAGCTGGTG; CTGATCCTAAGTGGGACAGTGGAGTCCACCAGTGCCACCTGTCAGGTGCGCACTTCCTAC; CTGCCAGAGGAGATCCTTTGGGGCTACGAGTTCACACCTGCCATCTCACTGTCAGCCAGT; GGTAAATACATAGCTGACTTTAGCCTTTTTGACCAAGTTGTGAAAGTGGCCTCTCCTAGT; GGCCTCCGTGACAGCACTGTACGCTACGGAGACCCTGAAAAGCTCAAGTTGGAGGAGTCA; TTAAGGGAGCAAGCTGAGAAGGAGGGCAGTGCCCTTAGTGTGCGCATCAGCAATGTCTGA; ; Clone variation with respect to NM_002241.4;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002241 unedited; ACGACTTCACTATAGGGCGGCCGCGATTCGGCACGAGGGCACAGCTCTGCTCCTAACTCC; TGCCCGCCCCGTCCGTCCATCTGTCCCGCTGCCCCGCGGCCCATCCAAGGGGCCACTCCA; CCTCGGACCCAAGATGACGTCAGTTGCCAAGGTGTATTACAGTCAGACCACTCAGACAGA; AAGCCGGCCCCTAATGGGCCCAGGGATACGACGGCGGAGAGTCCTGACAAAAGATGGTCG; CAGCAACGTGAGAATGGAGCACATTGCCGACAAGCGCTTCCTCTACCTCAAGGACCTGTG; GACAACCTTCATTGACATGCAGTGGCGCTACAAGCTTCTGCTCTTCTCTGCGACCTTTGC; AGGCACATGGTTCCTCTTTGGCGTGGTGTGGTATCTGGTAACTGTGGCACATGGGGACCT; GCTGGAGCTGGACCCCCCGGGCAACCACACCCCCTGTGTGGTACAGGTGCACACACTCAC; TGGAGCCTTGCTCTTGTGCCTTGAATCCAAACCACCATTGGGTATGGGTTACGGTACATA; AGTGAGGAATGTGCACTGGACATTGTGCTTGTTATTGAGCAGGTGGTGCTGGCCACCATG; CTGAGAATCTAGATGCAAGATCCTTGCTGGGGAAAATTGACCGGACGAAAACGGGAGTGA; AACCGTGGTTTGGAAGCGCGTGCGGAGTGGGGACTGTGGCAATGGGTGATGCGTGCGTGT; GAAGCAGGTGATGTACTTGGCGGAGACCGGGCTAGGTTGTTGGGTTGATGGACNGATGAA; CTATGGTGGTGCGTGGGCCAGGGGTGAGGGGTGGACCCTGTGCTGGAGCCGCGCGCTTGT; GAGTGTGTGGGTGGCCCCCGCGGCGAGAGGGGCGGGCTTAAGACCAGGCCAGGAGGNNTG; GTGGGGGGNAGCGGGACGGATATAAGCTCGTTACACAACTATGGATAAAAGAACGATATA; AAAGATAAGACTAANATATTC;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002241 unedited; GTACCGCGGCACGCATTTCTAGTAGTCGAGNNNTTTTTTTTTTTTTTTTTTTTTTTTAGG; AAACAAAAGGGGCTTTATTTTATTTTATTTATTTTAAAAAGGCGCTACAGTGGCAGGACG; CTGCTGGGGATGGGGACACACAGTGTAAGTGGGAGATTTTCCAAGACTTCCCCTCCCTAT; TGGCCTCCGAGTTCCTCTCTTCCTGGGCCTATCAGTAGCATAGGATTCAACAAAAGCCTT; CATGAATGCCTCTTTATTTCTGAAAAGGAACTTCTACCCCTCCCTTTCCCATTTCCAGAC; TTGAAATCCTTCATCAGCTATCTCACTTCCATGTTGAAACCAGGACCACTGATTGTAAGT; CTGAAAATCTGGGTGTCCTACCCCATAACCAGGCCAAGATATCTGCCCCCAATGCTAAAG; GCTCTGAATATTTCCTTTCTGGACATTCAATATATTTGGGCATATATACATCTATATACA; TCCTTCTATATATATATTATCTCTGGTTCTTTCTTCTTAGATAAACCCTTCATATATGGC; ATATCCTAATTTCCCAAGCCCTCCCACTCTTTGTTTGCGAGCCTAAGCAAGACTCAAGAG; TTTCATTGATTTCACTAAGGTCCCCAGCATCATTGCTTTCTCATCTTTCTACCCCAAGAT; TCTTCCCCATCACGTGTTTCCCCCCCTTCCGAAAACCCCCTTTTTAACACCGTGCCTACC; GAAATTCCCCTTTTTAAACTGGGCCTCTACTCTGCTGATTTCCAAGCCCCAGTGGGTTGG; GGGCCCATTTCTAACAAACCTGGCGGGTTCTAAGCCGGCCTATGGCTAAAGAAGATTAGG; GCCAAGGGAAAAACATTGTCCAAAAACCCTTTAAGTAAGGAAGGGGGACTTGCCCCCGCG; CAGGGCCTCGGGGAAAAAACCCATCCCCTTTCAGGACACGGGTTTAAGTGCCCCTTAACG; AACCCGGGGAAACCGCAGGATCT;
• Restriction Sites: NotI-NotI
• ACCN: NM_002241
• Insert Size: 4600 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_002241.2, NP_002232.2
• RefSeq Size: 4515 bp
• RefSeq ORF: 1140 bp
• Locus ID: 3766
• UniProt ID: P78508
• Cytogenetics: 1q23.2
• Domains: IRK
• Protein Families: Druggable Genome, Ion Channels: Potassium, Transmembrane
• Summary: This gene encodes a member of the inward rectifier-type potassium channel family, characterized by having a greater tendency to allow potassium to flow into, rather than out of, a cell. The encoded protein may form a heterodimer with another potassium channel protein and may be responsible for the potassium buffering action of glial cells in the brain. Mutations in this gene have been associated with seizure susceptibility of common idiopathic generalized epilepsy syndromes. [provided by RefSeq, Jul 2008]