NFkB p100 / p52 (NFKB2) (NM_002502) Human Untagged Clone

CAT#: SC118626

NFKB2 (untagged)-Human nuclear factor of kappa light polypeptide gene enhancer in B-cells 2 (p49/p100) (NFKB2), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NFkB p100 / p52
• Synonyms: CVID10; H2TF1; LYT-10; LYT10; NF-kB2; p49/p100; p52; p100
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118626 sequence for NM_002502 edited (data generated by NextGen Sequencing)
  ATGGAGAGTTGCTACAACCCAGGTCTGGATGGTATTATTGAATATGATGATTTCAAATTG
  AACTCCTCCATTGTGGAACCCAAGGAGCCAGCCCCAGAAACAGCTGATGGCCCCTACCTG
  GTGATCGTGGAACAGCCTAAGCAGAGAGGCTTTCGATTTCGATATGGCTGTGAAGGCCCC
  TCCCATGGAGGACTGCCCGGTGCCTCCAGTGAGAAGGGCCGAAAGACCTATCCCACTGTC
  AAGATCTGTAACTACGAGGGACCAGCCAAGATCGAGGTGGACCTGGTAACACACAGTGAC
  CCACCTCGTGCTCATGCCCACAGTCTGGTGGGCAAGCAATGCTCGGAGCTGGGGATCTGC
  GCCGTTTCTGTGGGGCCCAAGGACATGACTGCCCAATTTAACAACCTGGGTGTCCTGCAT
  GTGACTAAGAAGAACATGATGGGGACTATGATACAAAAACTTCAGAGGCAGCGGCTCCGC
  TCTAGGCCCCAGGGCCTTACGGAGGCCGAGCAGCGGGAGCTGGAGCAAGAGGCCAAAGAA
  CTGAAGAAGGTGATGGATCTGAGTATAGTGCGGCTGCGCTTCTCTGCCTTCCTTAGAGCC
  AGTGATGGCTCCTTCTCCCTGCCCCTGAAGCCAGTCATCTCCCAGCCCATCCATGACAGC
  AAATCTCCGGGGGCATCAAACCTGAAGATTTCTCGAATGGACAAGACAGCAGGCTCTGTG
  CGGGGTGGAGATGAAGTTTATCTGCTTTGTGACAAGGTGCAGAAAGATGACATTGAGGTT
  CGGTTCTATGAGGATGATGAGAATGGATGGCAGGCCTTTGGGGACTTCTCTCCCACAGAT
  GTGCATAAACAGTATGCCATTGTGTTCCGGACACCCCCCTATCACAAGATGAAGATTGAG
  CGGCCTGTAACAGTGTTTCTGCAACTGAAACGCAAGCGAGGAGGGGACGTGTCTGATTCC
  AAACAGTTCACCTATTACCCTCTGGTGGAAGACAAGGAAGAGGTGCAGCGGAAGCGGAGG
  AAGGCCTTGCCCACCTTCTCCCAGCCCTTCGGGGGTGGCTCCCACATGGGTGGAGGCTCT
  GGGGGTGCAGCCGGGGGCTACGGAGGAGCTGGAGGAGGTGGCAGCCTCGGTTTCTTCCCC
  TCCTCCCTGGCCTACAGCCCCTACCAGTCCGGCGCGGGCCCCATGGGCTGCTACCCGGGA
  GGCGGGGGCGGGGCGCAGATGGCCGCCACGGTGCCCAGCAGGGACTCCGGGGAGGAAGCC
  GCGGAGCCGAGCGCCCCCTCCAGGACCCCCCAGTGCGAGCCGCAGGCCCCGGAGATGCTG
  CAGCGAGCTCGAGAGTACAACGCGCGCCTGTTCGGCCTGGCGCAGCGCAGCGCCCGAGCC
  CTACTCGACTACGGCGTCACCGCGGACGCGCGCGCGCTGCTGGCGGGACAGCGCCACCTG
  CTGACGGCGCAGGACGAGAACGGAGACACACCACTGCACCTAGCCATCATCCACGGGCAG
  ACCAGTGTCATTGAGCAGATAGTCTATGTCATCCACCACGCCCAGGACCTCGGCGTTGTC
  AACCTCACCAACCACCTGCACCAGACGCCCCTGCACCTGGCGGTGATCACGGGGCAGACG
  AGTGTGGTGAGCTTTCTGCTGCGGGTAGGTGCAGACCCAGCTCTGCTGGATCGGCATGGA
  GACTCAGCCATGCATCTGGCGCTGCGGGCAGGCGCTGGTGCTCCTGAGCTGCTGCGTGCA
  CTGCTTCAGAGTGGAGCTCCTGCTGTGCCCCAGCTGTTGCATATGCCTGACTTTGAGGGA
  CTGTATCCAGTACACCTGGCGGTCCGAGCCCGAAGCCCTGAGTGCCTGGATCTGCTGGTG
  GACAGTGGGGCTGAAGTGGAGGCCACAGAGCGGCAGGGGGGACGAACAGCCTTGCATCTA
  GCCACAGAGATGGAGGAGCTGGGGTTGGTCACCCATCTGGTCACCAAGCTCCGGGCCAAC
  GTGAACGCTCGCACCTTTGCGGGAAACACACCCCTGCACCTGGCAGCTGGACTGGGGTAC
  CCGACCCTCACCCGCCTCCTTCTGAAGGCTGGTGCTGACATCCATGCTGAAAACGAGGAG
  CCCCTGTGCCCACTGCCTTCACCCCCTACCTCTGATAGCGACTCGGACTCTGAAGGGCCT
  GAGAAGGACACCCGAAGCAGCTTCCGGGGCCACACGCCTCTTGACCTCACTTGCAGCACC
  AAGGTGAAGACCTTGCTGCTAAATGCTGCTCAGAACACCATGGAGCCACCCCTGACCCCG
  CCCAGCCCAGCAGGGCCGGGACTGTCACTTGGTGATACAGCTCTGCAGAACCTGGAGCAG
  CTGCTAGACGGGCCAGAAGCCCAGGGCAGCTGGGCAGAGCTGGCAGAGCGTCTGGGGCTG
  CGCAGCCTGGTAGACACGTACCGACAGACAACCTCACCCAGTGGCAGCCTCCTGCGCAGC
  TACGAGCTGGCTGGCGGGGACCTGGCAGGTCTACTGGAGGCCCTGTCTGACATGGGCCTA
  GAGGAGGGAGTGAGGCTGCTGAGGGGTCCAGAAACCCGAGACAAGCTGCCCAGCACAGAG
  GTGAAGGAAGACAGTGCGTACGGGAGCCAGTCAGTGGAGCAGGAGGCAGAGAAGCTGGGC
  CCACCCCCTGAGCCACCAGGAGGGCTCTGCCACGGGCACCCCCAGCCTCAGGTGCACTGA
  
  Clone variation with respect to NM_002502.3
  153 c=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002502 unedited
  NGGTCANCATTTGTATACGACTCATATAGGGCGGCCGCGATTCGGCACGAGGCTCCACCG
  GATCTCACCCGCCACACCCGGCCAGGCGGCTGGAGGAGGCGGGCGTCTAAAATTCTGGGA
  AGCAGAACCTGGCCGGAGCCACTAGACAGAGCCGGGCCTAGCCCAGAGACATGGAGAGTT
  GCTACAACCCAGGTCTGGATGGTATTATTGAATATGATGATTTCAAATTGAACTCCTCCA
  TTGTGGAACCCAAGGAGCCAGCCCCAGAAACAGCTGATGGCCCCTACCTGGTGATCGTGG
  AACAGCCTAAGCAGAGAGGCTTTCGATTTCGATATGGCTGTGAAGGCCCCTCCCATGGAG
  GACTGCCCGGTGCCTCCAGTGAGAAGGGCCGAAAGACCTATCCCACTGTCAAGATCTGTA
  ACTACGAGGGACCAGCCAAGATCGAGGTGGACCTGGTAACACACAGTGACCCACCTCGTG
  CTCATGCCCACAGTCTGGTGGGCAAGCAATGCTCGGAGCTGGGGATCTGCGCCGTTTCTG
  TGGGGCCCAAGGACATGACTGCCCAATTTAACAACCTGGGTGTCCTGCATGTGACTAAGA
  AGAACATGATGGGGACTATGATACAAAAACTTCAGAGGCAGCGGCTCCGCTCTAGGCCCC
  AGGGCCTTACGGAGGCCGAGCAGCGGGAGCTGGAGCAAGAGCCAAAGAACTGAAGAAGGT
  GATGGATCTGAGTATAGTGCGGCTGCGCTTCTCTGCCTTCCTTAGAGCCAGNGATGGCTC
  CTNTCTCCTGCCCCTGAAGCCAGTCATCTTCCAGCCCATCCATGACAGCAAATCTCCGGG
  GGCATCAAACCTGNAAGATTCTCGAATGGACAAGACAGCAGGCTCTGTGCGGGGTGGAGT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002502 unedited
  GACCGCGGCCCGCTTCTAGNATCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTTTAAGGGGGGAAGGAGGGGTCCTCCCCTTCCCATGAAAATCCTTTAT
  TTGTCCCAACTGAGGGGTGGGTGTGGGGTGTTAAATAAAATTTGAAATAGGGGGGGACCC
  TGTACAGGGGGTCCGGGAAGGGGGCTGGGGGCAGGCAACAGGTCAGTGCACCTGAGGCTG
  GGGGTGCCCGTGGCAAAACCCTCCTGGTGGCTCAGGGGGTGGGCCCAGCTTTTTTGCCTC
  CTGCTCCACTGACTGGCTCCCGTACGCACTGTCTTCCTTCACCTTTGGGCTGGGCAGCTT
  GTCTCGGGTTTTTGGACCCCTCAGCAGCCTCACTCCCTCCTTTAGGCCCATGTCAAACAG
  GGCCTCCAGTAAACCTGCCAGGTCCCCGCCAGCCAGCTCGTAACTGCGCAGGAGGCTGCC
  ACTGGGTGAGGTTGTCTGTCGGTACGTGTCTACCAGGCTGCGCAGCCCCAGACGCTCTGC
  CAGCTCTGCCCAGCTGCCCTGGGCTTTTGGCCCGTCTAGCAGCTGCTCCAGGTTCTGCAG
  AGCTGTATCACCAAGTGACAGTCCCGGCCCTGCTGGGCTGGGCGGGGTCAGGGGTGGCTT
  CATGGGGTTCTGAGCAGCATCTAGCAGCAAGGGCTTCACCTTGGTGCTGCAAGTGAGGTC
  AAGAGGCGTGTGGCCCCGGAAGCTGCTTCGGGTGGCCTTCTCAGGCCTTTAAAGTCCGAT
  CGCTTTAAAGTACGGGGTGGAAGCAGGGGCCACAGAGTTTTTTCTTCTTCTTTGGTTGTA
  CCCCCACTCCTCTAGGAAGCGGTTGAGGTTCTCCACCCCCTATACATCCACAGCATGTGT
  GTCCCACACCACAGGTATTTACTTTACCATTTAACAACCN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002502
• Insert Size: 2900 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002502.2, NP_002493.2
• RefSeq Size: 3001 bp
• RefSeq ORF: 2802 bp
• Locus ID: 4791
• UniProt ID: Q00653
• Cytogenetics: 10q24.32
• Domains: RHD, DEATH, ANK, IPT
• Protein Families: Transcription Factors
• Protein Pathways: MAPK signaling pathway, Pathways in cancer
• Gene Summary: This gene encodes a subunit of the transcription factor complex nuclear factor-kappa-B (NFkB). The NFkB complex is expressed in numerous cell types and functions as a central activator of genes involved in inflammation and immune function. The protein encoded by this gene can function as both a transcriptional activator or repressor depending on its dimerization partner. The p100 full-length protein is co-translationally processed into a p52 active form. Chromosomal rearrangements and translocations of this locus have been observed in B cell lymphomas, some of which may result in the formation of fusion proteins. There is a pseudogene for this gene on chromosome 18. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2013]
  Transcript Variant: This variant (2) differs in the 5' UTR and uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. The encoded isoform (b) is shorter than isoform a. Variants 2, 3, and 4 encode the same isoform (b). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.