PABP (PABPC1) (NM_002568) Human Untagged Clone

CAT#: SC118549

PABPC1 (untagged)-Human poly(A) binding protein, cytoplasmic 1 (PABPC1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PABP
• Synonyms: PAB1; PABP; PABP1; PABPC2; PABPL1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118549 sequence for NM_002568 edited (data generated by NextGen Sequencing)
  ATGAACCCCAGTGCCCCCAGCTACCCCATGGCCTCGCTCTACGTGGGGGACCTCCACCCC
  GACGTGACCGAGGCGATGCTCTACGAGAAGTTCAGCCCGGCCGGGCCCATCCTCTCCATC
  CGGGTCTGCAGGGACATGATCACCCGCCGCTCCTTGGGCTACGCGTATGTGAACTTCCAG
  CAGCCGGCGGACGCGGAGCGTGCTTTGGACACCATGAATTTTGATGTTATAAAGGGCAAG
  CCAGTACGCATCATGTGGTCTCAGCGTGATCCATCACTTCGCAAAAGTGGAGTAGGCAAC
  ATATTCATTAAAAATCTGGACAAATCCATTGATAATAAAGCACTGTATGATACATTTTCT
  GCTTTTGGTAACATCCTTTCATGTAAGGTGGTTTGTGATGAAAATGGTTCCAAGGGCTAT
  GGATTTGTACACTTTGAGACGCAGGAAGCAGCTGAAAGAGCTATTGAAAAAATGAATGGA
  ATGCTCCTAAATGATCGCAAAGTATTTGTTGGACGATTTAAGTCTCGTAAAGAACGAGAA
  GCTGAACTTGGAGCTAGGGCAAAAGAATTCACCAATGTTTACATCAAGAATTTTGGAGAA
  GACATGGATGATGAGCGCCTTAAGGATCTCTTTGGCAAGTTTGGGCCTGCCTTAAGTGTG
  AAAGTAATGACTGATGAAAGTGGAAAATCCAAAGGATTTGGATTTGTAAGCTTTGAAAGG
  CATGAAGATGCACAGAAAGCTGTGGATGAGATGAACGGAAAGGAGCTCAATGGAAAACAA
  ATTTATGTTGGTCGAGCTCAGAAAAAGGTGGAACGGCAGACGGAACTTAAGCGCAAATTT
  GAACAGATGAAACAAGATAGGATCACCAGATACCAGGGTGTTAATCTTTATGTGAAAAAT
  CTTGATGATGGTATTGATGATGAACGTCTCCGGAAAGAGTTTTCTCCATTTGGTACAATC
  ACTAGTGCAAAGGTTATGATGGAGGGTGGTCGCAGCAAAGGGTTTGGTTTTGTATGTTTC
  TCCTCCCCAGAAGAAGCCACTAAAGCAGTTACAGAAATGAACGGTAGAATTGTGGCCACA
  AAGCCATTGTATGTAGCTTTAGCTCAGCGCAAAGAAGAGCGCCAGGCTCACCTCACTAAC
  CAGTATATGCAGAGAATGGCAAGTGTACGAGCTGTTCCCAACCCTGTAATCAACCCCTAC
  CAGCCAGCACCTCCTTCAGGTTACTTCATGGCAGCTATCCCACAGACTCAGAACCGTGCT
  GCATACTATCCTCCTAGCCAAATTGCTCAACTAAGACCAAGTCCTCGCTGGACTGCTCAG
  GGTGCCAGACCTCATCCATTCCAAAATATGCCCGGTGCTATCCGCCCAGCTGCTCCTAGA
  CCACCATTTAGTACTATGAGACCAGCTTCTTCACAGGTTCCACGAGTCATGTCAACACAG
  CGTGTTGCTAACACATCAACACAGACAATGGGTCCACGTCCTGCAGCTGCAGCCGCTGCA
  GCTACTCCTGCTGTCCGCACCGTTCCACAGTATAAATATGCTGCAGGAGTTCGCAATCCT
  CAGCAACATCTTAATGCACAGCCACAAGTTACAATGCAACAGCCTGCTGTTCATGTACAA
  GGTCAGGAACCTTTGACTGCTTCCATGTTGGCATCTGCCCCTCCTCAAGAGCAAAAGCAA
  ATGTTGGGTGAACGGCTGTTTCCTCTTATTCAAGCCATGCACCCTACTCTTGCTGGTAAA
  ATCACTGGCATGTTGTTGGAGATTGATAATTCAGAACTTCTTCATATGCTCGAGTCTCCA
  GAGTCACTCCGTTCTAAGGTTGATGAAGCTGTAGCTGTACTACAAGCCCACCAAGCTAAA
  GAGGCTGCCCAGAAAGCAGTTAACAGTGCCACCGGTGTTCCAACTGTTTAA
  
  Clone variation with respect to NM_002568.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002568 unedited
  NGGTCANAATTTGTATACGACTCATATAGGCGGCCGCGNAATTCGCACGAGGTAAAAAAC
  CCCAAAAAAATTACAAAAAATCCGCGTCTCCCCCGCCGGAGACTTTTATTTTTTTTCTTC
  CTCTTTTATAAAATAACCCGGTGAAGCAGCCGAGACCGACCCGCCCGCCCGCGGCCCCGC
  AGCAGCTCCAAGAAGGAACCAAGAGACCGAGGCCTTCCCGCTGCCCGGACCCGACACCGC
  CACCCTCGCTCCCCGCCGGCAGCCGGCAGCCAGCGGCAGTGGATCGACCCCGTTCTGCGG
  CCGTTGAGTAGTTTTCAATTCCGGTTGATTTTTGTCCCTCTGCGCTTGCTCCCCGCTCCC
  CTCCCCCCGGCTCCGGCCCCCAGCCCCGGCACTCGCTCTCCTCCTCTCACGGAAAGGTCG
  CGGCCTGTGGCCCTGCGGGCAGCCGTGCCGAGATGAACCCCAGTGCCCCCAGCTACCCCA
  TGGCCTCGCTCTACGTGGGGGACCTCCACCCCGACGTGACCGAGGCGATGCTCTACGAGA
  AGTTCAGCCCGGCCGGGCCCATCCTCTCCATCCGGGTCTGCAGGGACATGATCACCCGCC
  GCTCCTTGGGCTACGCGTATGTGAACTTCCAGCAGCCGGCGGACGCGGAGCGTGCTTTGG
  ACACCATGAATTTTGATGTTATAAAGGGCAAGCCAGTACGCATCATGTGGTCTCAGCGTG
  ATCCATCACTTCGCANAAGTGGAGTAGGCAACATATTCATTAAAAATCTGGACAAATCCA
  TTGATAATAAAGCACTGTATGATACATTNTCTGCTTTTGGGTACATCCTTTCATGTAGGT
  GGNTTGTGATGAAAATGGTCCAAGGCTATGGATTTGACACTCTGAGACGCAGAACAGCTA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002568 unedited
  CGGCCGCAATCTAGNATCGAGTTTTTTTTTTTTTTTTTTATTTATTTTATATTTTGCAAT
  GTTTTTTTTCCATAATATTTAAGTTTTTCGATGTTTAAATATTTTTCTTCGGTGAAGCAC
  AAGTTTCTTTTCATGGTCCCTGATCAATTTTAAACAGTTGGAACACCGGTGGCACTGTTA
  ACTGCTTTCTGGGCAGCCTCTTTAGCTTGGTGGGCTTGTAGTACAGCTACAGCTTCATCA
  ACCTTAGAACGGAGTGACTCTGGAGACTCGAGCATATGAAGAAGTTCTGAATTATCAATC
  TCCAACAACATGCCAGTGATTTTACCAGCAAGAGTAGGGTGCATGGCTTGAATAAGAGGA
  AACAGCCGTTCACCCAACATTTGCTTTTGCTCTTGAGGAGGGGCAGATGCCAACATGGAA
  GCAGTCAAAGGTTCCTGACCTTGTACATGAACAGCAGGCTGTTGCATTGTAACTTGTGGC
  TGTGCATTAAGATGTTGCTGAGGATTGCGAACTCCTGCAGCATATTTATACTGTGGAACG
  GTGCGGACAGCAGGAGTAGCTGCAGCGGCTGCAGCTGCAGGACGTGGACCCATTGTCTGT
  GTTGATGTGTTAGCAACACGCTGTGTTGACATGACTCGTGGAACCTGTGAAGAAGCTGGT
  CTCATAGTACTAAATGGTGGTCTANGAGCAGCTGGGCGGATAGCACCCNGGCATATTTGG
  AATGGATGANGTCTGGCACCCTGAGCAGTCCAGCGAGGACTTGGTCTTANNTGAGCAATT
  TGCTANGAAGAAGTATGCACCACGGNTCTGAGTCTGTGGGATAACTGCATGAAAAACCTT
  AAGAGTGCCTGCCTGTAGGGGNTGATACAGGTTTGGGACACNTCGACACCTGCCATCCTG
  CATAACTGGNTANGGAGGGAGCCTGGCGCTCTTCTTGGCCGAGCAAACTACTACAGGGCT
  TGGA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002568
• Insert Size: 2570 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002568.3, NP_002559.2
• RefSeq Size: 2869 bp
• RefSeq ORF: 1911 bp
• Locus ID: 26986
• UniProt ID: P11940
• Cytogenetics: 8q22.3
• Domains: RRM, PABP, RRM_1
• Gene Summary: This gene encodes a poly(A) binding protein. The protein shuttles between the nucleus and cytoplasm and binds to the 3' poly(A) tail of eukaryotic messenger RNAs via RNA-recognition motifs. The binding of this protein to poly(A) promotes ribosome recruitment and translation initiation; it is also required for poly(A) shortening which is the first step in mRNA decay. The gene is part of a small gene family including three protein-coding genes and several pseudogenes.[provided by RefSeq, Aug 2010]