DNA Polymerase gamma (POLG) (NM_002693) Human Untagged Clone

CAT#: SC118521

POLG (untagged)-Human polymerase (DNA directed), gamma (POLG), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DNA Polymerase gamma
• Synonyms: MDP1; MIRAS; MTDPS4A; MTDPS4B; PEO; POLG1; POLGA; SANDO; SCAE
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_002693, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAGCCGCCTGCTCTGGAGGAAGGTGGCCGGCGCCACCGTCGGGCCAGGGCCGGTTCCAGCTCCGGGGC
  GCTGGGTCTCCAGCTCCGTCCCCGCGTCCGACCCCAGCGACGGGCAGCGGCGGCGGCAGCAGCAGCAGCA
  GCAGCAGCAGCAGCAGCAACAGCAGCCTCAGCAGCCGCAAGTGCTATCCTCGGAGGGCGGGCAGCTGCGG
  CACAACCCATTGGACATCCAGATGCTCTCGAGAGGGCTGCACGAGCAAATCTTCGGGCAAGGAGGGGAGA
  TGCCTGGCGAGGCCGCGGTGCGCCGCAGCGTCGAGCACCTGCAGAAGCACGGGCTCTGGGGGCAGCCAGC
  CGTGCCCTTGCCCGACGTGGAGCTGCGCCTGCCGCCCCTCTACGGGGACAACCTGGACCAGCACTTCCGC
  CTCCTGGCCCAGAAGCAGAGCCTGCCCTACCTGGAGGCGGCCAACTTGCTGTTGCAGGCCCAGCTGCCCC
  CGAAGCCCCCGGCTTGGGCCTGGGCGGAGGGCTGGACCCGGTACGGCCCCGAGGGGGAGGCCGTACCCGT
  GGCCATCCCCGAGGAGCGGGCCCTGGTGTTCGACGTGGAGGTCTGCTTGGCAGAGGGAACTTGCCCCACA
  TTGGCGGTGGCCATATCCCCCTCGGCCTGGTATTCCTGGTGCAGCCAGCGGCTGGTGGAAGAGCGTTACT
  CTTGGACCAGCCAGCTGTCGCCGGCTGACCTCATCCCCCTGGAGGTCCCTACTGGTGCCAGCAGCCCCAC
  CCAGAGAGACTGGCAGGAGCAGTTAGTGGTGGGGCACAATGTTTCCTTTGACCGAGCTCATATCAGGGAG
  CAGTACCTGATCCAGGGTTCCCGCATGCGTTTCCTGGACACCATGAGCATGCACATGGCCATCTCAGGGC
  TAAGCAGCTTCCAGCGCAGTCTGTGGATAGCAGCCAAGCAGGGCAAACACAAGGTCCAGCCCCCCACAAA
  GCAAGGCCAGAAGTCCCAGAGGAAAGCCAGAAGAGGCCCAGCGATCTCATCCTGGGACTGGCTGGACATC
  AGCAGTGTCAACAGTCTGGCAGAGGTGCACAGACTTTATGTAGGGGGGCCTCCCTTAGAGAAGGAGCCTC
  GAGAACTGTTTGTGAAGGGCACCATGAAGGACATTCGTGAGAACTTCCAGGACCTGATGCAGTACTGTGC
  CCAGGACGTGTGGGCCACCCATGAGGTTTTCCAGCAGCAGCTACCGCTCTTCTTGGAGAGGTGTCCCCAC
  CCAGTGACTCTGGCCGGCATGCTGGAGATGGGTGTCTCCTACCTGCCTGTCAACCAGAACTGGGAGCGTT
  ACCTGGCAGAGGCACAGGGCACTTATGAGGAGCTCCAGCGGGAGATGAAGAAGTCGTTGATGGATCTGGC
  CAATGATGCCTGCCAGCTGCTCTCAGGAGAGAGGTACAAAGAAGACCCCTGGCTCTGGGACCTGGAGTGG
  GACCTGCAAGAATTTAAGCAGAAGAAAGCTAAGAAGGTGAAGAAGGAACCAGCCACAGCCAGCAAGTTGC
  CCATCGAGGGGGCTGGGGCCCCTGGTGATCCCATGGATCAGGAAGACCTCGGCCCCTGCAGTGAGGAGGA
  GGAGTTTCAACAAGATGTCATGGCCCGCGCCTGCTTGCAGAAGCTGAAGGGGACCACAGAGCTCCTGCCC
  AAGCGGCCCCAGCACCTTCCTGGACACCCTGGATGGTACCGGAAGCTCTGCCCCCGGCTAGACGACCCTG
  CATGGACCCCGGGCCCCAGCCTCCTCAGCCTGCAGATGCGGGTCACACCTAAACTCATGGCACTTACCTG
  GGATGGCTTCCCTCTGCACTACTCAGAGCGTCATGGCTGGGGCTACTTGGTGCCTGGGCGGCGGGACAAC
  CTGGCCAAGCTGCCGACAGGTACCACCCTGGAGTCAGCTGGGGTGGTCTGCCCCTACAGAGCCATCGAGT
  CCCTGTACAGGAAGCACTGTCTCGAACAGGGGAAGCAGCAGCTGATGCCCCAGGAGGCCGGCCTGGCGGA
  GGAGTTCCTGCTCACTGACAATAGTGCCATATGGCAAACGGTAGAAGAACTGGATTACTTAGAAGTGGAG
  GCTGAGGCCAAGATGGAGAACTTGCGAGCTGCAGTGCCAGGTCAACCCCTAGCTCTGACTGCCCGTGGTG
  GCCCCAAGGACACCCAGCCCAGCTATCACCATGGCAATGGACCTTACAACGACGTGGACATCCCTGGCTG
  CTGGTTTTTCAAGCTGCCTCACAAGGATGGTAATAGCTGTAATGTGGGAAGCCCCTTTGCCAAGGACTTC
  CTGCCCAAGATGGAGGATGGCACCCTGCAGGCTGGCCCAGGAGGTGCCAGTGGGCCCCGTGCTCTGGAAA
  TCAACAAAATGATTTCTTTCTGGAGGAACGCCCATAAACGTATCAGCTCCCAGATGGTGGTGTGGCTGCC
  CAGGTCAGCTCTGCCCCGTGCTGTGATCAGGCACCCCGACTATGATGAGGAAGGCCTCTATGGGGCCATC
  CTGCCCCAAGTGGTGACTGCCGGCACCATCACTCGCCGGGCTGTGGAGCCCACATGGCTCACCGCCAGCA
  ATGCCCGGCCTGACCGAGTAGGCAGTGAGTTGAAAGCCATGGTGCAGGCCCCACCTGGCTACACCCTTGT
  GGGTGCTGATGTGGACTCCCAAGAGCTGTGGATTGCAGCTGTGCTTGGAGACGCCCACTTTGCCGGCATG
  CATGGCTGCACAGCCTTTGGGTGGATGACACTGCAGGGCAGGAAGAGCAGGGGCACTGATCTACACAGTA
  AGACAGCCACTACTGTGGGCATCAGCCGTGAGCATGCCAAAATCTTCAACTACGGCCGCATCTATGGTGC
  TGGGCAGCCCTTTGCTGAGCGCTTACTAATGCAGTTTAACCACCGGCTCACACAGCAGGAGGCAGCTGAG
  AAGGCCCAGCAGATGTACGCTGCCACCAAGGGCCTCCGCTGGTATCGGCTGTCGGATGAGGGCGAGTGGC
  TGGTGAGGGAGTTGAACCTCCCAGTGGACAGGACTGAGGGTGGCTGGATTTCCCTGCAGGATCTGCGCAA
  GGTCCAGAGAGAAACTGCAAGGAAGTCACAGTGGAAGAAGTGGGAGGTGGTTGCTGAACGGGCATGGAAG
  GGGGGCACAGAGTCAGAAATGTTCAATAAGCTTGAGAGCATTGCTACGTCTGACATACCACGTACCCCGG
  TGCTGGGCTGCTGCATCAGCCGAGCCCTGGAGCCCTCGGCTGTCCAGGAAGAGTTTATGACCAGCCGTGT
  GAATTGGGTGGTACAGAGCTCTGCTGTTGACTACTTACACCTCATGCTTGTGGCCATGAAGTGGCTGTTT
  GAAGAGTTTGCCATAGATGGGCGCTTCTGCATCAGCATCCATGACGAGGTTCGCTACCTGGTGCGGGAGG
  AGGACCGCTACCGCGCTGCCCTGGCCTTGCAGATCACCAACCTCTTGACCAGGTGCATGTTTGCCTACAA
  GCTGGGTCTGAATGACTTGCCCCAGTCAGTCGCCTTTTTCAGTGCAGTCGATATTGACCGGTGCCTCAGG
  AAGGAAGTGACCATGGATTGTAAAACCCCTTCCAACCCAACTGGGATGGAAAGGAGATACGGGATTCCCC
  AGGGTGAAGCGCTGGATATTTACCAGATAATTGAACTCACCAAAGGCTCCTTGGAAAAACGAAGCCAGCC
  TGGACCATAG
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002693 unedited
  GTCAGCTTTTGTATACGACTCCTATAGGGCGGCCGCGAATTCGCACGAGGGGAAGTTGCG
  GCTGCCAGCGAAGCGGACCGGCCGGGTGGAGGCCACACGCTACCCCGAGGCTGCGTAGGC
  CGCGCGAAGGGGGACGCCGTGCCGTGGGCCTGGGGTCGGGGGAGCAGCAGACCGGGAAGC
  ACCGATTTGGGGTGGAAGGCAGGCATGGTCAAACCCATTTCACTGACAGGAGAGCAGAGA
  CAGGACGTGTCTCTCTCCACGTCTTCCAGCCAGTAAAAGAAGCCAAGCTGGAGCCCAAAG
  CCAGGTGTTCTGACTCCCAGCGTGGGGGTCCCTGCACCAACCATGAGCCGCCTGCTCTGG
  AGGAAGGTGGCCGGCGCCACCGTCGGGCCAGGGCCGGTTCCAGCTCCGGGGCGCTGGGTC
  TCCAGCTCCGTCCCCGCGTCCGACCCCAGCGACGGGCAGCGGCGGCGGCAGCAGCAGCAG
  CAGCAGCAGCAGCAGCAGCAACAGCAGCCTCAGCAGCCGCAAGTGCTATCCTCGGAGGGC
  GGGCAGCTGCGGCACAACCCATTGGACATCCAGATGCTCTCGAGAGGGCTGCACGAGCAA
  ATCTTCGGGCAAGGGAGGGGAGATGCCTGGCGAGCNCGCGGTGCGCCGCAGCGTCGAGCA
  CCTGCAGAAGCACGGGCTCTGGNNGGCAGCCAGCCGTGCCCTTGCCCGACGTGGNAGCTG
  CGCCTGCCGCCCCTCTACGGNNGACACCTGGACCAGCACTTCCGCCTCCTGGCCCAGAAG
  CAGAGCCTGCCTACCTGGNAGCGGCAACTTGTGTGTTTGCAGGCCCAGCTGNCCCGAGCC
  CCCGCTTGCTGTNNNNNNNNNNNGNNTNTTTNCCCCCCCTGCCCNCCCGGGGGGGGGGGC
  CCCTCCCCGTGGCCTTCCCGGGGGACGGCCTTG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002693 unedited
  ATGTACGCGGCCGCAATCTATATCGAGCTCTTTTTTTTTTTTTTTTTTTTTTTTCCTTCT
  TTTTATTTCCACTGCCTTGGAGCAGGTTTATCACGTTAAAGCATTAATTCTTTCCCCTTC
  TAGGGCACTGCATCAAAGCATGGGGGACAGAACAAAGAACCAGCCAAGAAGAAAAGGAAA
  AAATAAATGAAATGCCTGAGTTAATGTGAACTTTGGGGCTTCTGCTTCATTTTTACCCAA
  CAAGCAACAATGCCCCTTGTCCTGTAGTCCACACCGATGTTGGCATCTTGGTTCTGAACC
  CACTGAATTCAACTGCACCTTCAGTTAAAAGGAATCTTCTTGGCAGGTCCTGCTACTGAA
  AAATGGCTGGCCTTAGGCAAGCCCTTTTGCAAAAAGCACAGCTGAAAGCCTGATTTTGGG
  AGCCTGCACCACCCCGATGAAGCTCCACGGGAGCAAATACAGAGCCTCCAGGCAGTGCTA
  TGGTCCAGGCTGGCTTAGTTTTTCCAAGGAGCCTTTGGCGAGTTCAATTATCTGGTAAAT
  ATCCAGCGCTTCACCCTGTGGAATCCCGTATCCTCTTTTCATCCCATTTGGGTTGGAAAG
  GGGTTTACAATCCATGGTCACTTCTTTCCTGAGGCACCGGTCAATTTAGACTGCCCCGCA
  AAAGGCCACTGACTTGGGCAAGTATTTCATACCAAGCTTGTAGGTAAACATGCACCTGGT
  ACATAAGGTTGTGTTCCCTGCATGCCCAGGCCATACTGTTCTTGCCCCCTCTCCCCCCCC
  ACTACCGTATCCTCCCCTGTTTGTTCAGCATCTACCCCTATTTTTGTTATACTCTTCTAT
  AGTCCCCTCTTCGATCACTACCCCGCCGGCCATTCCTTATAGGAACCACTCCGTACAACA
  GTATTTACAGCCTGTTATCTTCTTTAACTCCACATCCCACACCCTACCTCCTCCCGCGCC
  CCCTCTCTCACCCTCCGCGCTCATATAATTCACCCCCAAGTATCCTATCCAT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002693
• Insert Size: 4110 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002693.1, NP_002684.1
• RefSeq Size: 4465 bp
• RefSeq ORF: 3720 bp
• Locus ID: 5428
• UniProt ID: P54098
• Cytogenetics: 15q26.1
• Domains: DNA_pol_A
• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways
• Gene Summary: Mitochondrial DNA polymerase is heterotrimeric, consisting of a homodimer of accessory subunits plus a catalytic subunit. The protein encoded by this gene is the catalytic subunit of mitochondrial DNA polymerase. The encoded protein contains a polyglutamine tract near its N-terminus that may be polymorphic. Defects in this gene are a cause of progressive external ophthalmoplegia with mitochondrial DNA deletions 1 (PEOA1), sensory ataxic neuropathy dysarthria and ophthalmoparesis (SANDO), Alpers-Huttenlocher syndrome (AHS), and mitochondrial neurogastrointestinal encephalopathy syndrome (MNGIE). Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) represents the longer transcript. Both variants encode the same protein.