POLD1 (NM_002691) Human Untagged Clone

CAT#: SC118520

POLD1 (untagged)-Human polymerase (DNA directed), delta 1, catalytic subunit 125kDa (POLD1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: POLD1
• Synonyms: CDC2; CRCS10; MDPL; POLD
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_002691, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGATGGCAAGCGGCGGCCAGGCCCAGGGCCCGGGGTGCCCCCAAAGCGGGCCCGTGGGGGCCTCTGGG
  ATGATGATGATGCACCTCGGCCATCCCAATTCGAGGAGGACCTGGCACTGATGGAGGAGATGGAGGCAGA
  ACACAGGCTGCAGGAGCAGGAGGAGGAGGAGCTGCAGTCAGTCCTGGAGGGGGTTGCAGACGGGCAGGTC
  CCACCATCAGCCATAGATCCTCGCTGGCTTCGGCCCACACCACCAGCGCTGGACCCCCAGACAGAGCCCC
  TCATCTTCCAACAGTTGGAGATTGACCATTATGTGGGCCCAGCGCAGCCTGTGCCTGGGGGGCCCCCACC
  ATCCCGCGGCTCCGTGCCTGTGCTCCGCGCCTTCGGGGTCACCGATGAGGGGTTCTCTGTCTGCTGCCAC
  ATCCACGGCTTCGCTCCCTACTTCTACACCCCAGCGCCCCCTGGTTTCGGGCCCGAGCACATGGGTGACC
  TGCAACGGGAGCTGAACTTGGCCATCAGCCGGGACAGTCGCGGGGGGAGGGAGCTGACTGGGCCGGCCGT
  GCTGGCTGTGGAACTGTGCTCCCGAGAGAGCATGTTTGGGTACCACGGGCACGGCCCCTCCCCGTTCCTG
  CGCATCACCGTGGCGCTGCCGCGCCTCGTGGCCCCGGCCCGCCGTCTCCTGGAACAGGGCATCCGTGTGG
  CAGGCCTGGGCACGCCCAGCTTCGCGCCCTACGAGGCCAACGTCGACTTTGAGATCCGGTTCATGGTGGA
  CACGGACATCGTCGGCTGCAACTGGCTGGAGCTCCCAGCTGGGAAATACGCCCTGAGGCTGAAGGAGAAG
  GCTACGCAGTGCCAGCTGGAGGCGGACGTGCTGTGGTCTGACGTGGTCAGTCACCCACCGGAAGGGCCAT
  GGCAGCGCATTGCGCCCTTGCGCGTGCTCAGCTTCGATATCGAGTGCGCCGGCCGCAAAGGCATCTTCCC
  TGAGCCTGAGCGGGACCCTGTCATCCAGATCTGCTCGCTGGGCCTGCGCTGGGGGGAGCCGGAGCCCTTC
  CTACGCCTGGCGCTCACCCTGCGGCCCTGTGCCCCCATCCTGGGTGCCAAGGTGCAGAGCTACGAGAAGG
  AGGAGGACCTGCTGCAGGCCTGGTCCACCTTCATCCGTATCATGGACCCCGACGTGATCACCGGTTACAA
  CATCCAGAACTTCGACCTTCCGTACCTCATCTCTCGGGCCCAGACCCTCAAGGTACAAACATTCCCTTTC
  CTGGGCCGTGTGGCCGGCCTTTGCTCCAACATCCGGGACTCTTCATTCCAGTCCAAGCAGACGGGCCGGC
  GGGACACCAAGGTTGTCAGCATGGTGGGCCGCGTGCAGATGGACATGCTGCAGGTGCTGCTGCGGGAGTA
  CAAGCTCCGCTCCTACACGCTCAATGCCGTGAGCTTCCACTTCCTGGGCGAGCAGAAGGAGGACGTGCAG
  CACAGCATCATCACCGACCTGCAGAATGGGAACGACCAGACCCGCCGCCGCCTGGCTGTGTACTGCCTGA
  AGGATGCCTACCTGCCACTGCGGCTGCTGGAGCGGCTCATGGTGCTGGTGAACGCCGTGGAGATGGCGAG
  GGTCACTGGCGTGCCCCTCAGCTACCTGCTCAGTCGTGGCCAGCAGGTCAAGGTCGTATCCCAGCTGTTG
  CGGCAGGCCATGCACGAGGGGCTGCTGATGCCCGTGGTGAAGTCAGAGGGCGGCGAGGACTACACGGGAG
  CCACTGTCATCGAGCCCCTCAAAGGGTACTACGACGTCCCCATCGCCACCCTGGACTTCTCCTCGCTGTA
  CCCGTCCATCATGATGGCCCACAACCTGTGTTACACCACGCTCCTTCGGCCCGGGACTGCACAGAAACTG
  GGCCTGACTGAGGATCAGTTCATCAGGACCCCCACCGGGGACGAGTTTGTGAAGACCTCAGTGCGGAAGG
  GGCTGCTGCCCCAGATCCTGGAGAACCTGCTCAGTGCCCGGAAGAGGGCCAAGGCCGAGCTGGCCAAGGA
  GACAGACCCCCTCCGGCGCCAGGTCCTGGATGGACGGCAGCTGGCGCTGAAGGTGAGCGCCAACTCCGTA
  TACGGCTTCACTGGCGCCCAGGTGGGCAAGTTGCCGTGCCTGGAGATCTCACAGAGCGTCACGGGGTTCG
  GACGTCAGATGATCGAGAAAACCAAGCAGCTGGTGGAGTCTAAGTACACAGTGGAGAATGGCTACAGCAC
  CAGTGCCAAGGTGGTGTATGGTGACACTGACTCCGTCATGTGCCGATTCGGCGTGTCCTCGGTGGCTGAG
  GCGATGGCCCTGGGGCGGGAGGCCGCGGACTGGGTGTCAGGTCACTTCCCGTCGCCCATCCGGCTGGAGT
  TTGAGAAGGTCTACTTCCCATACCTGCTTATCAGCAAGAAGCGCTACGCGGGCCTGCTCTTCTCCTCCCG
  GCCCGACGCCCACGACCGCATGGACTGCAAGGGCCTGGAGGCCGTGCGCAGGGACAACTGCCCCCTCGTG
  GCCAACCTGGTCACTGCCTCACTGCGCCGCCTGCTCATCGACCGAGACCCTGAGGGCGCGGTGGCTCACG
  CACAGGACGTCATCTCGGACCTGCTGTGCAACCGCATCGATATCTCCCAGCTGGTCATCACCAAGGAGCT
  GACCCGCGCGGCCTCCGACTATGCCGGCAAGCAGGCCCACGTGGAGCTGGCCGAGAGGATGAGGAAGCGG
  GACCCCGGGAGTGCGCCCAGCCTGGGCGACCGCGTCCCCTACGTGATCATCAGTGCCGCCAAGGGTGTGG
  CCGCCTACATGAAGTCGGAGGACCCGCTGTTCGTGCTGGAGCACAGCCTGCCCATTGACACGCAGTACTA
  CCTGGAGCAGCAGCTGGCCAAGCCCCTCCTGCGCATCTTCGAGCCCATCCTGGGCGAGGGCCGTGCCGAG
  GCTGTGCTACTGCGGGGGGACCACACGCGCTGCAAGACGGTGCTCACGGGCAAGGTGGGCGGCCTCCTGG
  CCTTCGCCAAACGCCGCAACTGCTGCATTGGCTGCCGCACAGTGCTCAGCCACCAGGGAGCCGTGTGTGA
  GTTCTGCCAGCCCCGGGAGTCTGAGCTGTATCAGAAGGAGGTATCCCATCTGAATGCCCTGGAGGAGCGC
  TTCTCGCGCCTCTGGACGCAGTGCCAGCGCTGCCAGGGCAGCCTGCACGAGGACGTCATCTGCACCAGCC
  GGGACTGCCCCATCTTCTACATGCGCAAGAAGGTGCGGAAGGACCTGGAAGACCAGGAGCAGCTCCTGCG
  GCGCTTCGGACCCCCTGGACCTGAGGCCTGGTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002691 unedited
  ATATCCCCCGCCCCGTTGCCGCAAAGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAA
  GCAGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCG
  CGAATTCGGCACGAGGGCGGCGTAGGCTGTGGCGGGAAACGCTGTTTGAAGCGGGATGGA
  TGGCAAGCGGCGGCCAGGCCCAGGGCCCGGGGTGCCCCCAAAGCGGGCCCGTGGGGGCCT
  CTGGGATGATGATGATGCACCTCGGCCATCCCAATTCGAGGAGGACCTGGCACTGATGGA
  GGAGATGGAGGCAGAACACAGGCTGCAGGAGCAGGAGGAGGAGGAGCTGCAGTCAGTCCT
  GGAGGGGGTTGCAGACGGGCAGGTCCCACCATCAGCCATAGATCCTCGCTGGCTTCGGCC
  CACACCACCAGCGCTGGACCCCCAGACAGAGCCCCTCATCTTCCAACAGTTGGAGATTGA
  CCATTATGTGGGCCCAGCGCAGCCTGTGCCTGGGGGGCCCCCACCATCCCGCGGCTCCGT
  GCCTGTGCTCCGCGCCTTCGGGGTCACCGATGAGGGGTTCTCTGTCTGCTGCCACATCCA
  CGGCTTCGCTCCCTACTTCTACACCCCAGCGCCCCCTGGTTTCGGGCCCGAGCACATGGG
  TGACCTGCAACGGGAGCTGAACTTGGCCATCAGCCGGGACAGTCGCGGGGGGAGGGGAGC
  TGACTGGGCCGGCCGTGCTGGCTGTGGAACTGTGCTCCCGAGAGAGCATGTTTGGGTACC
  ACGGGCACGNGCCCTCCCCGTTCCTGCGCTCANCGTTGGCGCTGCCGCGCCTCGTGGCCC
  CGGCCCGCCGTCTCCTGGGACAGGGCATCCGTGTGGCAGGCCTGGGCACGCCCAGCTTCG
  CGCCCTACGAGCCNACGTCN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002691 unedited
  CATATTATGTACCGCGCCGCATTCTANGATCGATTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTAACAAAAGTCCAAAACTTTATTAATTCTCCCTGGTCCCGCCCCCGCCC
  CATGGGATGCTTGCAAGGTCACCAAGCCTCAAGTCCAGGGGGTCCGAAACGCCGCAAGAG
  CTGCTCCTGGTCTTCCAGGTCCTTCCGCACCTTTTTGCGCATGTAAAAAATGGGGCAGTC
  CCGGCTGGTGCAAATGACGTCCTCGTGCAAGCTGCCCTGGCAGCGCTGGCACTGCGTCCA
  AAGGCGCGAGAAGCGCTCCTCCAGGGCATTCAAATGGGATACCTCCTTCTGATACAGCTC
  AAACTCCCGGGGCTGGCAGAACTCACACACGGCTCCCTGGTGGCTGAGCACTGTGCGGCA
  GCCAATGCAACATTTGCGGCGTTTGGCGAAAGCCAAGAAGCCGCCCACCTTGCCCGTGAA
  CACCGTCTTGCAGCGCGTGTGGTCCCCCCGCAGTAGCACAACCTCGGCACGGCCCTCGCC
  CAAGATGGGCTCGAATATGCGCAAGAAGGGCTTGGCCAACTGCTGCTCCAAGTAGTACTG
  CGTGTCAATGGGCAAGCTGTGCTCCAGCACGAACAGCGGGTCCTCCGACTTTATGTAAGC
  GGGCACACCCTTGGCGGGACTGATGATCACCTATGGGACGCGGGTCCCCAAGCTGGGCGC
  ACTTCCAGGGTCCCGCTTTCTCATACTCTTGGGCAACTCCACGTGGGCCTTGTTGCCNGC
  TTATTCGGAGGGCCCCCGGGTCAACTTCTTGTGGATGACCCACTGGGAGATATCGATTGC
  GTTTGCCAACAAGGTCCCAAAAACCTCCCGTGCGTGACCCACCGGCCCCCCAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002691
• Insert Size: 3600 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002691.1, NP_002682.1
• RefSeq Size: 3443 bp
• RefSeq ORF: 3324 bp
• Locus ID: 5424
• UniProt ID: P28340
• Cytogenetics: 19q13.33
• Domains: DNA_pol_B_exo, DNA_pol_B, POLBc
• Protein Families: Druggable Genome, Stem cell - Pluripotency
• Protein Pathways: Base excision repair, DNA replication, Homologous recombination, Metabolic pathways, Mismatch repair, Nucleotide excision repair, Purine metabolism, Pyrimidine metabolism
• Gene Summary: This gene encodes the 125-kDa catalytic subunit of DNA polymerase delta. DNA polymerase delta possesses both polymerase and 3' to 5' exonuclease activity and plays a critical role in DNA replication and repair. Alternatively spliced transcript variants have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 6. [provided by RefSeq, Mar 2012]
  Transcript Variant: This variant (2) contains an alternate exon in the 5' UTR and uses an alternate splice site in the 3' coding region, compared to variant 4. The encoded isoform (1) is shorter than isoform 2.