PSMD9 (NM_002813) Human Untagged Clone

CAT#: SC118384

PSMD9 (untagged)-Human proteasome (prosome, macropain) 26S subunit, non-ATPase, 9 (PSMD9)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PSMD9
• Synonyms: p27; Rpn4
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118384 sequence for NM_002813 edited (data generated by NextGen Sequencing)
  ATGTCCGACGAGGAAGCGAGGCAGAGCGGAGGCTCCTCGCAGGCCGGCGMCGTGACTGTC
  AGCGACGTCCAGGAGCTGATGCGGCGCAAGGAGGAGATAGAAGCGCAGATCAAGGCCAAC
  TATGACGTGCTGGAAAGCCAAAAAGGCATTGGGATGAACGAGCCGCTGGTGGACTGTGAG
  GGCTACCCCCGGTCAGACGTGGACCTGTACCAAGTCCGCACCGCCAGGCACAACATCATA
  TGCCTGCAGAATGATCACAAGGCAGTGATGAAGCAGGTGGAGGAGGCCCTGCACCAGCTG
  CACGCTCGCGACAAGGAGAAGCAGGCCCGGGACATGGCTGAGGCCCACAAAGAGGCCATG
  AGCCGCAAACTGGGTCAGAGTGAGAGCCAGGGCCCTCCACGGGCCTTCGCCAAAGTGAAC
  AGCATCAGCCCCGGCTCCCCAGCCAGCATCGCGGGTCTGCAAGTGGATGATGAGATTGTG
  GAGTTCGGCTCTGTGAACACCCAGAACTTCCAGTCACTGCATAACATTGGCAGTGTGGTG
  CAGCACAGTGAGGGGAAGCCCCTGAATGTGACAGTGATCCGCAGGGGGGAAAAACACCAG
  CTTAGACTTGTTCCAACACGCTGGGCAGGAAAAGGACTGCTGGGCTGCAACATTATTCCT
  CTGCAAAGATGA
  
  Clone variation with respect to NM_002813.4
  50 t=>m
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002813 unedited
  ATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGTCCCTAGCCCG
  GGAGCCGGGTCTCTGGAGTCGCGGCCCGGGGTTCACGATGTCCGACGAGGAAGCGAGGCA
  GAGCGGAGGCTCCTCGCAGGCCGGCGCCGTGACTGTCAGCGACGTCCAGGAGCTGATGCG
  GCGCAAGGAGGAGATAGAAGCGCAGATCAAGGCCAACTATGACGTGCTGGAAAGCCAAAA
  AGGCATTGGGATGAACGAGCCGCTGGTGGACTGTGAGGGCTACCCCCGGTCAGACGTGGA
  CCTGTACCAAGTCCGCACCGCCAGGCACAACATCATATGCCTGCAGAATGATCACAAGGC
  AGTGATGAAGCAGGTGGAGGAGGCCCTGCACCAGCTGCACGCTCGCGACAAGGAGAAGCA
  GGCCCGGGACATGGCTGAGGCCCACAAAGAGGCCATGAGCCGCAAACTGGGTCAGAGTGA
  GAGCCAGGGCCCTCCACGGGCCTTCGCCAAAGTGAACAGCATCAGCCCCGGCTCCCCAGC
  CAGCATCGCGGGTCTGCAAGTGGATGATGAGATTGTGGAGTTCGGCTCTGTGAACACCCA
  GAACTTCCAGTCACTGCATAACATTGGCAGTGTGGTGCAGCACAGTGAGGGGAAGCCCCT
  GAATGTGACAGTGATCCGCAGGGGGGAAAAACACCAGCTTAGACTTGTTCCCACACGCTG
  GGCANGAAAAGGACTGCTGGGCTGCAACATTATTCCTCTGCAAGATGATTGTCCCTGGGG
  AAACAGTACAGGNAAGCATCTTTCCCTTGCCCTGGACTNGGGTCTAGGGGATTTCCACTT
  GTCNTCTCTNCCCTGAGCAATAAGATCTGGNAAGAGGCCTTGTACCTGAACCTCTGN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002813 unedited
  CCGCGGCCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTACATATCAGCACTGTAAA
  ACTCTTTATTAAAAATAATAATAATAATCACACCAGTACCTGCCAGATAACTTCCGCCCA
  CTTGCCCACAATCCAGGGAATTCCTATTTAGGGCCGTGGCCTAATTTGTGAAAGAGGCCA
  AGCCTAAGTTTTTAAGAATGCCTTAATCCAGGGAGATTACACTGGTGGGCCACAGTACTG
  CCACCACACAGAAGTTCAGGTTACAAGCCTCTTCCAGATCCTTATGCTTCAGGGAGAGAA
  GACAAGTTGGAAATCCCTAGACCCAAGTCCAGGGCAAGGGAAGATGCTTTCCTGTTACTG
  TTCCCCAGGGACAATCATCTTTGCAGAGGAATAATGTTGCAGCCCAGCAGTCCTTTTCCT
  GCCCAGCGTGTTGGAACAAGTCTAAGCTGGTGTTTTTCCCCCCTGCGGATCACTGTCACA
  TTCAGGGGCTTCCCCTCACTGTGCTGCACCACACTGCCAATGTTATGCAGTGACTGGAAG
  TTCTGGGTGTTCACAGAGCCGAACTCCACAATCTCATCATCCACTTGCANACCCGCGATG
  CTGGCTGGGGAGCCGGGGCTGATGCTGTTCACTTTGGCGAAAGCCCGTGGAGGGCCCTGG
  CTCTCACTTCTGACCCCATTTGCGGTTCATGGCCCTCTTTGTGGGCCTCAGCCATGTCCC
  GGGGCCTGTTNTTTCTTGTCCCGAGCGTGCACCTGGTGCAAGGCCTTCTCCACCTGCCAA
  ATAAATGCCTTGTGATCATTTTGCAGGCATATGAATGTTGGGCCTGGCGGGGCCGACTTG
  GTACCATGTCCAACTTTTGACCGGGGGGTAGCCTTAACAACCAACACGGGGCTTGTTATT
  TCCATGGCCTTTTTGCCTTTCCCACCGTCATATATGGCCTTGATCTGCGCTATTATT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002813
• Insert Size: 1140 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002813.4, NP_002804.2
• RefSeq Size: 2360 bp
• RefSeq ORF: 672 bp
• Locus ID: 5715
• UniProt ID: O00233
• Cytogenetics: 12q24.31
• Domains: PDZ
• Gene Summary: The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a non-ATPase subunit of the 19S regulator. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, May 2012]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1).