RGS2 (NM_002923) Human Untagged Clone

CAT#: SC118352

RGS2 (untagged)-Human regulator of G-protein signaling 2, 24kDa (RGS2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RGS2
• Synonyms: G0S8
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_002923 edited
  ATGCAAAGTGCTATGTTCTTGGCTGTTCAACACGACTGCAGACCCATGGACAAGAGCGCA
  GGCAGTGGCCACAAGAGCGAGGAGAAGCGAGAAAAGATGAAACGGACCCTTTTAAAAGAT
  TGGAAGACCCGTTTGAGCTACTTCTTACAAAATTCCTCTACTCCTGGGAAGCCCAAAACC
  GGCAAAAAAAGCAAACAGCAAGCTTTCATCAAGCCTTCTCCTGAGGAAGCACAGCTGTGG
  TCAGAAGCATTTGACGAGCTGCTAGCCAGCAAATATGGTCTTGCTGCATTCAGGGCTTTT
  TTAAAGTCGGAATTCTGTGAAGAAAATATTGAATTCTGGCTGGCCTGTGAAGACTTCAAA
  AAAACCAAATCACCCCAAAAGCTGTCCTCAAAAGCAAGGAAAATATATACTGACTTCATA
  GAAAAGGAAGCTCCAAAAGAGATAAACATAGATTTTCAAACCAAAACTCTGATTGCCCAG
  AATATACAAGAAGCTACAAGTGGCTGCTTTACAACTGCCCAGAAAAGGGTATACAGCTTG
  ATGGAGAACAACTCTTATCCTCGTTTCTTGGAGTCAGAATTCTACCAGGACTTGTGTAAA
  AAGCCACAAATCACCACAGAGCCTCATGCTACATGA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002923 unedited
  AGCATATTTGTATACGACTCACTATAGGGCGGCNCGCGAATTCGCACGAGGGGGAGAACG
  ATAATGCAAAGTGCTATGTTCTTGGCTGTTCAACACGACTGCAGACCCATGGACAAGAGC
  GCAGGCAGTGGCCACAAGAGCGAGGAGAAGCGAGAAAAGATGAAACGGACCCTTTTAAAA
  GATTGGAAGACCCGTTTGAGCTACTTCTTACAAAATTCCTCTACTCCTGGGAAGCCCAAA
  ACCGGCAAAAAAAGCAAACAGCAAGCTTTCATCAAGCCTTCTCCTGAGGAAGCACAGCTG
  TGGTCAGAAGCATTTGACGAGCTGCTAGCCAGCAAATATGGTCTTGCTGCATTCAGGGCT
  TTTTTAAAGTCGGAATTCTGTGAAGAAAATATTGAATTCTGGCTGGCCTGTGAAGACTTC
  AAAAAAACCAAATCACCCCAAAAGCTGTCCTCAAAAGCAAGGAAAATATATACTGACTTC
  ATAGAAAAGGAAGCTCCAAAAGAGATAAACATAGATTTTCAAACCAAAACTCTGATTGCC
  CAGAATATACAAGAAGCTACAAGTGGCTGCTNTACAACTGCCCAGAANAGGGTATACAGC
  TTGATGGAGAACAACTCTTATCCTCGTTTCTTGGAGTCAGAATTCTACCAGGACTTGTGN
  TAAAAGCCACANATCACCACAGAGCCTCATGCTACATGAAATGTANAAGGGAGCCCCAGA
  ATGGAGGACATTTCATTCTTTNTCCTGAGGGGAAGGACTGTGACCTGCCATANAGACTGA
  CCTTGNATNCAGCCTGGGTGTTCAGGNAACATCACTCAGAACTATTGATCCAAAGNTGGG
  GTAGTGAATCAGGAAGCCCAGTACTGACTAGGAGAAGCTGGTATCAGAACAGCTTNCCTC
  ACTGTGTACAGACGCCAGAGGGNATAGGTGTCTGAACGTGTGTCTCATCTGAAAGCAGAA
  TGTAGATGAG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002923 unedited
  CAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTAAATATTATTTATCAAAATAAATTTAT
  TAAAAGTATTCAAAGACCACTTCAAAGTGTAGCTGCCTTCAAGACAGATTTTTGGCACTC
  ATAACGGACACTGCAGTTTTCAACACCATAGCACTCATTCTATTTCACACATCATTTTTA
  ACAATGCAAACACGGACCATTTCAGTTTTAGCATTACATGAGACAACAGTACTGATGATC
  TGTGGACATAAAAACTTCAATACCGTTGCACATAGTAAACACTTAACTGTTACTGAATCC
  TAAACTAAAACTACTATGTGGTAACATGGATCGATTAAGGGAAAGATGTACAACCAGCTA
  CCTAAGGCCACATAATCCCAGATCTATTGATTTTAAATGCTTTTGGACCAACAGTATTAC
  ATTGTCTCTTTCATCATCTTACATTCCTGCTTTTCAGAGTGAGACACCACGTTCAGACCA
  CCTATTCCCTTCTTGCGTTCTGTACACAGTGAGGGAAGCTGTTCTGATACCAGCTTCTCC
  TAGTCAGTTACTGGCTTCCTGATTCCTACCCAACTTTGAATCAATAGTTCTGAGTGATGT
  TTCCTAGACACTCCAGCTGAATTCAAGGTCAGTCTTTATGGAAGGTCACAGTGCTTCCCC
  TCAGGAAAAGAACGAAATGTCCTCCATTTCTGGGCTCACTTTACATTCATGTACATGAGG
  CTCGGTGGGATTTGTGCCTTTTACCAGTCCTGGAAATTCTGACTCCAGAACGAGATAGAG
  CTGTCTCATCAGCTGTAACCCTTTCTGCCACNTGAAAGCACCCCTTGTATTCTTGTTATC
  TGGCATACAGTTTGTTGAAAACTAGTNATCTTTTGAGCTGCTTTCAGAGCAGAAATTTTC
  CTGTTTGAGACACTTGGGGGATTGCTTTTGAACTCACAGCCCCCATAAATTTCTCCACAT
  CCAT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002923
• Insert Size: 1330 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002923.1, NP_002914.1
• RefSeq Size: 1345 bp
• RefSeq ORF: 636 bp
• Locus ID: 5997
• UniProt ID: P41220
• Cytogenetics: 1q31.2
• Domains: RGS
• Protein Families: Druggable Genome
• Gene Summary: Regulator of G protein signaling (RGS) family members are regulatory molecules that act as GTPase activating proteins (GAPs) for G alpha subunits of heterotrimeric G proteins. RGS proteins are able to deactivate G protein subunits of the Gi alpha, Go alpha and Gq alpha subtypes. They drive G proteins into their inactive GDP-bound forms. Regulator of G protein signaling 2 belongs to this family. The protein acts as a mediator of myeloid differentiation and may play a role in leukemogenesis. [provided by RefSeq, Aug 2009]