SPARC (NM_003118) Human Untagged Clone

CAT#: SC118167

SPARC (untagged)-Human secreted protein, acidic, cysteine-rich (osteonectin) (SPARC)


  "NM_003118" in other vectors (6)

Reconstitution Protocol

USD 450.00

In Stock*

Size
    • 10 ug

Product Images

Frequently bought together (3)
SPARC mouse monoclonal antibody, clone OTI2B1 (formerly 2B1)
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Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
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USD 59.00

Other products for "SPARC"

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol SPARC
Synonyms BM-40; OI17; ON; ONT
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC118167 sequence for NM_003118 edited (data generated by NextGen Sequencing)
ATGAGGGCCTGGATCTTCTTTCTCCTTTGCCTGGCCGGGAGGGCCTTGGCAGCCCCTNNN
NNAGAAGCCCTGCCTGATGAGACAGAGGTGGTGGAAGAAACTGTGGCAGAGGTGACTGAG
GTATCTGTGGGAGCTAATCCTGTCCAGGTGGAAGTAGGAGAATTTGATGATGGTGCAGAG
GAAACCGAAGAGGAGGTGGTGGCGGAAAATCCCTGCCAGAACCACCACTGCAAACACGGC
AAGGTGTGCGAGCTGGATGAGAACAACACCCCCATGTGCGTGTGCCAGGACCCCACCAGC
TGCCCAGCCCCCATTGGCGAGTTTGAGAAGGTGTGCAGCAATGACAACAAGACCTTCGAC
TCTTCCTGCCACTTCTTTGCCACAAAGTGCACCCTGGAGGGCACCAAGAAGGGCCACAAG
CTCCACCTGGACTACATCGGGCCTTGCAAATACATCCCCCCTTGCCTGGACTCTGAGCTG
ACCGAATTCCCCCTGCGCATGCGGGACTGGCTCAAGAACGTCCTGGTCACCCTGTATGAG
AGGGATGAGGACAACAACCTTCTGACTGAGAAGCAGAAGCTGCGGGTGAAGAAGATCCAT
GAGAATGAGAAGCGCCTGGAGGCAGGAGACCACCCCGTGGAGCTGCTGGCCCGGGACTTC
GAGAAGAACTATAACATGTACATCTTCCCTGTACACTGGCAGTTCGGCCAGCTGGACCAG
CACCCCATTGACGGGTACCTCTCCCACACCGAGCTGGCTCCACTGCGTGCTCCCCTCATC
CCCATGGAGCATTGCACCACCCGCTTTTTCGAGACCTGTGACCTGGACAATGACAAGTAC
ATCGCCCTGGATGAGTGGGCCGGCTGCTTCGGCATCAAGCAGAAGGATATCGACAAGGAT
CTTGTGATCTAA

Clone variation with respect to NM_003118.2
58 c=>n;59 a=>n;60 g=>n;61 c=>n;62 a=>n
>OriGene 5' read for NM_003118 unedited
GTCGCATTTGTATACGACTCCTATAGGGCGGCCGCGAATCGGCACCAGCTCCACATTCCC
GCGGTCCTTCAGACTGCCCGGAGAGCGCGCTCTGCCTGCCGCCTGCCTGCCTGCCACTGA
GGGTTCCCAGCACCATGAGGGCCTGGATCTTCTTTCTCCTTTGCCTGGCCGGGAGGGCCT
TGGCAGCCCCTCAAGAAGCCCTGCCTGATGAGACAGAGGTGGTGGAAGAAACTGTGGCAG
AGGTGACTGAGGTATCTGTGGGAGCTAATCCTGTCCAGGTGGAAGTAGGAGAATTTGATG
ATGGTGCAGAGGAAACCGAAGAGGAGGTGGTGGCGGAAAATCCCTGCCAGAACCACCACT
GCAAACACGGCAAGGTGTGCGAGCTGGATGAGAACAACACCCCCATGTGCGTGTGCCAGG
ACCCCACCAGCTGCCCAGCCCCCATTGGCGAGTTTGAGAAGGTGTGCAGCAATGACAACA
AGACCTTCGACTCTTCCTGCCACTTCTTTGCCACAAAGTGCACCCTGGAGGGCACCAAGA
AGGGCCACAAGCTCCACCTGGACTACATCGGGCCTTGCAAATACATCCCCCCTTGCCTGG
ACTCTGAGCTGACCGAATTCCCCCTGCGCATGCGGGACTGGCTCAAGAACGTCCTGGTCA
CCCTGTATGAGAGGGATGAGGACAACAACCTTCTGACTGAGAAGCAGAAGCTGCGGGTGA
AGAAGATCCATGAGAATGAGAAGCGCCTGGNAGGCAGAGACCACCCCGTGGAGCTGCTGG
CCCCGGACTTCGAGAAGACTATAACATGTACATCCTCCCTGTACACTGGCAGTTCGGCCA
GCTGGACCAGCACCCCATTGACGGGTACCTCTCCCACACCGAGCTGGCTCCATGCGTGCT
CCCCTCATCCCATGGAGCATGCAC
>OriGene 3' read for NM_003118 unedited
CTATGGACCGCGGCACGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTTGATTTTTTA
GGAGCTTTTATTGTTTTAGTAATCTTAACATAACTTAAAATAAGAGAGGGGAAATGACAT
CTGGAGATCTAGGTATGTGGCCCATTGCAATTGAGCACATTTCTTGGGTCTGTTTCTCTA
TCTCTAAGGGGAGTCTCAAAACCCCAGCTCAAAATACGACACTAACATGATGAACATGCA
TGAGCTTTGAAAAGTGCTCTGTAGTCTTATGATGATCTAGAAGAGCACTGTCCAATAGAA
CTTTCTGTGATGATGAAAAGATTCTACTTCTGACCTATTCAATAGGGTAACCACTAATCA
TGCATGGCTCTCAAGCACTTGAAATGTTGCTAGTGTGATTGGGGAGCTGCGTTTTGAATG
TTAACTAATTTAAATTTAAATCTTAAAAAGTTACATGTGGGTAGTGGCTGCCGTACGGAA
CAGTACAGCACTAGAATAATGCTGTGTCCTCCTGTTACACATACTCATGGTCTCTCCTAC
TCTCCCTTCATCACACCTGTGACATCTTGCAATTTATATTTGATTGTGTCATTATCTAAT
GTATTCCTCCTGGACTCTAAGCTCCAAATGAACAAATGCTTTGTCTACTTTGCCATAACT
GTGTCCCCAGTGCTTGGCATGGGACCTGGTGCANAGTAAATACATCATAAATATTTTGTG
AATGAATGAATGAATGAATGACCANNTGATAATAAAAGATATAGCTGCTCANTCTGGTGC
TGATATTGGGGGANATGATCAAAAGGGTAAACCACAGGTCCTTAAATGAAAACAGCTAAC
TTATTGCTTACAAGAAACCTACCCTCCCTGGGTATAAACTGCCCGGTGTTTGTGGTCCCG
GGTGGTGTGTGCAAAAACATAATGGGGTTGCAAAGGGTGCATTTGGGGAACCATGGTAAA
CAAGATGCCCTTGGGTTTTTGGGGAAGAAAATAAAGGAACTAAAA
Restriction Sites NotI-NotI     
ACCN NM_003118
Insert Size 3280 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_003118.2, NP_003109.1
RefSeq Size 3178 bp
RefSeq ORF 912 bp
Locus ID 6678
UniProt ID P09486
Cytogenetics 5q33.1
Domains kazal, FOLN
Protein Families Druggable Genome
Gene Summary This gene encodes a cysteine-rich acidic matrix-associated protein. The encoded protein is required for the collagen in bone to become calcified but is also involved in extracellular matrix synthesis and promotion of changes to cell shape. The gene product has been associated with tumor suppression but has also been correlated with metastasis based on changes to cell shape which can promote tumor cell invasion. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2015]
Transcript Variant: This variant (1) encodes the predominant isoform (1).

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