Munc18 1 (STXBP1) (NM_003165) Human Untagged Clone

CAT#: SC118145

STXBP1 (untagged)-Human syntaxin binding protein 1 (STXBP1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Munc18 1
• Synonyms: DEE4; MUNC18-1; N-Sec1; NSEC1; P67; RBSEC1; unc-18A; UNC18; unc18-1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118145 sequence for NM_003165 edited (data generated by NextGen Sequencing)
  ATGGCCCCCATTGGCCTCAAAGCTGTTGTCGGAGAGAAGATTATGCATGATGTGATAAAG
  AAGGTCAAGAAGAAGGGGGAATGGAAGGTGCTGGTGGTGGATCAGTTAAGCATGAGGATG
  CTGTCCTCCTGCTGCAAGATGACAGACATCATGACCGAGGGCATAACGATTGTGGAAGAT
  ATCAATAAGCGCAGAGAGCCGCTCCCCAGCCTGGAGGCTGTGTATCTCATCACTCCATCC
  GAGAAGTCCGTCCACTCTCTCATCAGTGACTTTAAGGACCCGCCGACTGCTAAATACCGG
  GCTGCACACGTCTTCTTCACTGACTCTTGTCCAGATGCCCTGTTTAATGAACTGGTAAAA
  TCCCGAGCAGCCAAAGTCATCAAAACTCTGACGGAAATCAATATTGCATTTCTCCCGTAT
  GAATCCCAGGTCTATTCCTTGGACTCTGCTGACTCTTTCCAAAGCTTCTACAGTCCCCAC
  AAGGCTCAGATGAAGAATCCTATACTGGAGCGCCTGGCAGAGCAGATCGCGACCCTTTGT
  GCCACCCTGAAGGAGTACCCGGCTGTGCGGTATCGGGGGGAATACAAGGACAATGCCCTG
  CTGGCTCAGCTAATCCAGGACAAGCTCGATGCCTATAAAGCTGATGATCCAACAATGGGG
  GAGGGCCCAGACAAGGCACGCTCCCAGCTCCTGATCCTGGATCGAGGCTTTGACCCCAGC
  TCCCCTGTGCTCCATGAATTGACTTTTCAGGCTATGAGTTATGATCTGCTGCCTATCGAA
  AATGATGTATACAAGTATGAGACCAGCGGCATCGGGGAGGCACGGGTGAAGGAGGTGCTC
  CTGGACGAGGACGACGACCTGTGGATAGCACTGCGCCACAAGCACATCGCAGAGGTGTCC
  CAGGAAGTCACCCGGTCTCTGAAAGATTTTTCTTCTAGCAAGAGAATGAATACTGGAGAG
  AAGACCACCATGCGGGACCTGTCCCAGATGCTGAAGAAGATGCCTCAGTACCAGAAAGAG
  CTCAGCAAGTACTCCACCCACCTGCACCTTGCTGAGGACTGTATGAAGCATTACCAAGGC
  ACCGTAGACAAACTCTGCCGAGTGGAGCAGGACCTGGCCATGGGCACAGATGCTGAGGGA
  GAGAAGATCAAGGACCCTATGCGAGCCATCGTCCCCATTCTGCTGGATGCCAATGTCAGC
  ACTTATGACAAAATCCGCATCATCCTTCTCTACATCTTTTTGAAGAATGGCATCACGGAG
  GAAAACCTGAACAAACTGATCCAGCACGCCCAGATACCCCCGGAGGATAGTGAGATCATC
  ACCAACATGGCTCACCTCGGCGTGCCCATCGTCACCGATTCCACGCTGCGTCGCCGGAGC
  AAGCCGGAGCGGAAGGAACGCATCAGCGAGCAGACCTACCAGCTCTCACGGTGGACTCCG
  ATTATCAAGGACATCATGGAGGACACTATTGAGGACAAACTTGACACCAAACACTACCCT
  TATATCTCTACCCGTTCCTCTGCCTCCTTCAGCACCACCGCCGTCAGCGCCCGCTATGGG
  CACTGGCATAAGAACAAGGCCCCAGGCGAGTACCGCAGTGGCCCCCGCCTCATCATTTTC
  ATCCTTGGGGGTGTGAGCCTGAATGAGATGCGCTGCGCCTACGAGGTGACCCAGGCCAAC
  GGAAAGTGGGAGGTGCTGATAGGTTCTACTCACATTCTTACTCCCACCAAATTTCTCATG
  GACCTGAGACACCCCGACTTCAGGGAGTCCTCTAGGGTATCTTTTGAGGATCAGGCTCCA
  ACAATGGAGTGA
  
  Clone variation with respect to NM_003165.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003165 unedited
  ATACGACTCACTATAGGGCGGCCGCGAATTCGCACGAGGGGAGCCGGGAGACTCGCGCAG
  CGCCATGGCCCCCATTGGCCTCAAAGCTGTTGTCGGAGAGAAGATTATGCATGATGTGAT
  AAAGAAGGTCAAGAAGAAGGGGGAATGGAAGGTGCTGGTGGTGGATCAGTTAAGCATGAG
  GATGCTGTCCTCCTGCTGCAAGATGACAGACATCATGACCGAGGGCATAACGATTGTGGA
  AGATATCAATAAGCGCAGAGAGCCGCTCCCCAGCCTGGAGGCTGTGTATCTCATCACTCC
  ATCCGAGAAGTCCGTCCACTCTCTCATCAGTGACTTTAAGGACCCGCCGACTGCTAAATA
  CCGGGCTGCACACGTCTTCTTCACTGACTCTTGTCCAGATGCCCTGTTTAATGAACTGGT
  AAAATCCCGAGCAGCCAAAGTCATCAAAACTCTGACGGAAATCAATATTGCATTTCTCCC
  GTATGAATCCCAGGTCTATTCCTTGGACTCTGCTGACTCTTTCCAAAGCTTCTACAGTCC
  CCACAAGGCTCAGATGAAGAATCCTATACTGGAGCGCCTGGCAGAGCAGATCGCGACCCT
  TTGTGCCACCCTGAAGGAGTACCCGGCTGTGCGGTATCGGGGGGAATACAAGGACAATGC
  CCTGCTGGCTCAGCTAATCCAGGACAAGCTCGATGCCTATAAAGCTGATGATCCAACATG
  GGGGGAGGGCCCAGACAGGCACGCTCCAGCTCCTGATCCTGGATCGAGGCTTTGACCCCA
  GCTCCCTGTGCTCATGAATTGACTTTTCAGCTATGAGTTATGATCTGCTGCTATCGAAAT
  GATGTATACAGTATGAGACAGCGCATCGGGAGCACGGTGAAANGAGTGCTCTGGGACAGA
  CNACACCTGTGNTAGCCTGCGCACAGCATGGNAAAGTGTCCAGGAGTCCCCGCTTTGAAG
  ATTTCTTTAAN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003165 unedited
  TGGACCGCGGGCCGCAATCTAGNATCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTAGGTTTGAGGGGGAATGCTGGAAATTGTAATGGGTATGGAAACATAT
  CATATAAGTAATGCTAGGGGGAGTGGTAGGAAATTTTTTCATAGGAGGGGTATGAATTGG
  TCGTACCGGAATCGGGGGTATGAAACCCTGGGAAAGGCAAAAAGGTCTGTTCCACCTTGG
  ACCTCCTGAAATATCCTGATTGTCACCTCCCAGGTTATTTGGATGAAAACAGGCCCTGAG
  GGGTAGGGACTGGAATGAAAATACCAAATTAGTCCTCAGTGGGAAATACTCCAAGAACAC
  AGACATCTCTATGATTCTCAACAAGGACAGGAACAACCCCAAACAAGGTTTTAAAAACAC
  CCAAATTTCTACCGTTCCATACCCAAGCAATGGGCACGTCACCCAGGGTTATGTACAAGG
  GCACAAATCTTAAATAACGGGGAGAAGGCGTCCATTCATCCTAACAGTCCAAAAATTTCT
  CCTGCTGTGCACCAATTGCACATGAGCTTCTCTTTTTAGGATACATGTATAAGATACTCT
  TTTTAAAAAACACAATGCATTATTTTCTTTTGTTTCAAACAAGAAGAGGGCCTTACTGAA
  CACGAGAATGCAGCGGCAACAGTGCCACCCAGGGCGTGCAAGCCTGGGGAGGCAACACAC
  GCCAAGGAACTGAGGCGGGCCGTGGTGGGAAACCTCCGAACCCTGTGGGATGGGGGGCTG
  TTTTTTGAAGGCCGACCTTATTTTTTAACCGCTTATTTCTTCATCGGGTTATTCAAGTCC
  TCCAAGGGTCCCACCATTTCTGTGGGGGGGAGATATTNTTTCGGAAACCCTTCCCCCCTC
  CC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003165
• Insert Size: 2500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003165.1, NP_003156.1
• RefSeq Size: 3899 bp
• RefSeq ORF: 1812 bp
• Locus ID: 6812
• UniProt ID: P61764
• Cytogenetics: 9q34.11
• Domains: Sec1
• Gene Summary: This gene encodes a syntaxin-binding protein. The encoded protein appears to play a role in release of neurotransmitters via regulation of syntaxin, a transmembrane attachment protein receptor. Mutations in this gene have been associated with infantile epileptic encephalopathy-4. Alternatively spliced transcript variants have been described. [provided by RefSeq, Feb 2010]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a).