Thrombospondin 1 (THBS1) (NM_003246) Human Untagged Clone

CAT#: SC118074

THBS1 (untagged)-Human thrombospondin 1 (THBS1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Thrombospondin 1
• Synonyms: THBS; THBS-1; TSP; TSP-1; TSP1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_003246, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGGGCTGGCCTGGGGACTAGGCGTCCTGTTCCTGATGCATGTGTGTGGCACCAACCGCATTCCAGAGT
  CTGGCGGAGACAACAGCGTGTTTGACATCTTTGAACTCACCGGGGCCGCCCGCAAGGGGTCTGGGCGCCG
  ACTGGTGAAGGGCCCCGACCCTTCCAGCCCAGCTTTCCGCATCGAGGATGCCAACCTGATCCCCCCTGTG
  CCTGATGACAAGTTCCAAGACCTGGTGGATGCTGTGCGGGCAGAAAAGGGTTTCCTCCTTCTGGCATCCC
  TGAGGCAGATGAAGAAGACCCGGGGCACGCTGCTGGCCCTGGAGCGGAAAGACCACTCTGGCCAGGTCTT
  CAGCGTGGTGTCCAATGGCAAGGCGGGCACCCTGGACCTCAGCCTGACCGTCCAAGGAAAGCAGCACGTG
  GTGTCTGTGGAAGAAGCTCTCCTGGCAACCGGCCAGTGGAAGAGCATCACCCTGTTTGTGCAGGAAGACA
  GGGCCCAGCTGTACATCGACTGTGAAAAGATGGAGAATGCTGAGTTGGACGTCCCCATCCAAAGCGTCTT
  CACCAGAGACCTGGCCAGCATCGCCAGACTCCGCATCGCAAAGGGGGGCGTCAATGACAATTTCCAGGGG
  GTGCTGCAGAATGTGAGGTTTGTCTTTGGAACCACACCAGAAGACATCCTCAGGAACAAAGGCTGCTCCA
  GCTCTACCAGTGTCCTCCTCACCCTTGACAACAACGTGGTGAATGGTTCCAGCCCTGCCATCCGCACTAA
  CTACATTGGCCACAAGACAAAGGACTTGCAAGCCATCTGCGGCATCTCCTGTGATGAGCTGTCCAGCATG
  GTCCTGGAACTCAGGGGCCTGCGCACCATTGTGACCACGCTGCAGGACAGCATCCGCAAAGTGACTGAAG
  AGAACAAAGAGTTGGCCAATGAGCTGAGGCGGCCTCCCCTATGCTATCACAACGGAGTTCAGTACAGAAA
  TAACGAGGAATGGACTGTTGATAGCTGCACTGAGTGTCACTGTCAGAACTCAGTTACCATCTGCAAAAAG
  GTGTCCTGCCCCATCATGCCCTGCTCCAATGCCACAGTTCCTGATGGAGAATGCTGTCCTCGCTGTTGGC
  CCAGCGACTCTGCGGACGATGGCTGGTCTCCATGGTCCGAGTGGACCTCCTGTTCTACGAGCTGTGGCAA
  TGGAATTCAGCAGCGCGGCCGCTCCTGCGATAGCCTCAACAACCGATGTGAGGGCTCCTCGGTCCAGACA
  CGGACCTGCCACATTCAGGAGTGTGACAAGAGATTTAAACAGGATGGTGGCTGGAGCCACTGGTCCCCGT
  GGTCATCTTGTTCTGTGACATGTGGTGATGGTGTGATCACAAGGATCCGGCTCTGCAACTCTCCCAGCCC
  CCAGATGAACGGGAAACCCTGTGAAGGCGAAGCGCGGGAGACCAAAGCCTGCAAGAAAGACGCCTGCCCC
  ATCAATGGAGGCTGGGGTCCTTGGTCACCATGGGACATCTGTTCTGTCACCTGTGGAGGAGGGGTACAGA
  AACGTAGTCGTCTCTGCAACAACCCCACACCCCAGTTTGGAGGCAAGGACTGCGTTGGTGATGTAACAGA
  AAACCAGATCTGCAACAAGCAGGACTGTCCAATTGATGGATGCCTGTCCAATCCCTGCTTTGCCGGCGTG
  AAGTGTACTAGCTACCCTGATGGCAGCTGGAAATGTGGTGCTTGTCCCCCTGGTTACAGTGGAAATGGCA
  TCCAGTGCACAGATGTTGATGAGTGCAAAGAAGTGCCTGATGCCTGCTTCAACCACAATGGAGAGCACCG
  GTGTGAGAACACGGACCCCGGCTACAACTGCCTGCCCTGCCCCCCACGCTTCACCGGCTCACAGCCCTTC
  GGCCAGGGTGTCGAACATGCCACGGCCAACAAACAGGTGTGCAAGCCCCGTAACCCCTGCACGGATGGGA
  CCCACGACTGCAACAAGAACGCCAAGTGCAACTACCTGGGCCACTATAGCGACCCCATGTACCGCTGCGA
  GTGCAAGCCTGGCTACGCTGGCAATGGCATCATCTGCGGGGAGGACACAGACCTGGATGGCTGGCCCAAT
  GAGAACCTGGTGTGCGTGGCCAATGCGACTTACCACTGCAAAAAGGATAATTGCCCCAACCTTCCCAACT
  CAGGGCAGGAAGACTATGACAAGGATGGAATTGGTGATGCCTGTGATGATGACGATGACAATGATAAAAT
  TCCAGATGACAGGGACAACTGTCCATTCCATTACAACCCAGCTCAGTATGACTATGACAGAGATGATGTG
  GGAGACCGCTGTGACAACTGTCCCTACAACCACAACCCAGATCAGGCAGACACAGACAACAATGGGGAAG
  GAGACGCCTGTGCTGCAGACATTGATGGAGACGGTATCCTCAATGAACGGGACAACTGCCAGTACGTCTA
  CAATGTGGACCAGAGAGACACTGATATGGATGGGGTTGGAGATCAGTGTGACAATTGCCCCTTGGAACAC
  AATCCGGATCAGCTGGACTCTGACTCAGACCGCATTGGAGATACCTGTGACAACAATCAGGATATTGATG
  AAGATGGCCACCAGAACAATCTGGACAACTGTCCCTATGTGCCCAATGCCAACCAGGCTGACCATGACAA
  AGATGGCAAGGGAGATGCCTGTGACCACGATGATGACAACGATGGCATTCCTGATGACAAGGACAACTGC
  AGACTCGTGCCCAATCCCGACCAGAAGGACTCTGACGGCGATGGTCGAGGTGATGCCTGCAAAGATGATT
  TTGACCATGACAGTGTGCCAGACATCGATGACATCTGTCCTGAGAATGTTGACATCAGTGAGACCGATTT
  CCGCCGATTCCAGATGATTCCTCTGGACCCCAAAGGGACATCCCAAAATGACCCTAACTGGGTTGTACGC
  CATCAGGGTAAAGAACTCGTCCAGACTGTCAACTGTGATCCTGGACTCGCTGTAGGTTATGATGAGTTTA
  ATGCTGTGGACTTCAGTGGCACCTTCTTCATCAACACCGAAAGGGACGATGACTATGCTGGATTTGTCTT
  TGGCTACCAGTCCAGCAGCCGCTTTTATGTTGTGATGTGGAAGCAAGTCACCCAGTCCTACTGGGACACC
  AACCCCACGAGGGCTCAGGGATACTCGGGCCTTTCTGTGAAAGTTGTAAACTCCACCACAGGGCCTGGCG
  AGCACCTGCGGAACGCCCTGTGGCACACAGGAAACACCCCTGGCCAGGTGCGCACCCTGTGGCATGACCC
  TCGTCACATAGGCTGGAAAGATTTCACCGCCTACAGATGGCGTCTCAGCCACAGGCCAAAGACGGGTTTC
  ATTAGAGTGGTGATGTATGAAGGGAAGAAAATCATGGCTGACTCAGGACCCATCTATGATAAAACCTATG
  CTGGTGGTAGACTAGGGTTGTTTGTCTTCTCTCAAGAAATGGTGTTCTTCTCTGACCTGAAATACGAATG
  TAGAGATCCCTAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003246 unedited
  GTATTTTGTAATACGAACTCACTATAGGGCGGCCGCGAATTCGCACGAGGCGAGTTCAGG
  GCTCCTGCCGCTCTCCAGNAGCAACCTCTACTCCGGACGCACAGGCATTCCCCGCGCCCC
  TCCAGCCCTCGCCGCCCTCGCCACCGCTCCCGGCCGCCGCGCTCCGGTACACACAGGATC
  CCTGCTGGGCACCAACAGCTCCACCATGGGGCTGGCCTGGGGACTAGGCGTCCTGTTCCT
  GATGCATGTGTGTGGCACCAACCGCATTCCAGAGTCTGGCGGAGACAACAGCGTGTTTGA
  CATCTTTGAACTCACCGGGGCCGCCCGCAAGGGGTCTGGGCGCCGACTGGTGAAGGGCCC
  CGACCCTTCCAGCCCAGCTTTCCGCATCGAGGATGCCAACCTGATCCCCCCTGTGCCTGA
  TGACAAGTTCCAAGACCTGGTGGATGCTGTGCGGGCAGAAAAGGGTTTCCTCCTTCTGGC
  ATCCCTGAGGCAGATGAAGAAGACCCGGGGCACGCTGCTGGCCCTGGAGCGGAAAGACCA
  CTCTGGCCAGGTCTTCAGCGTGGTGTCCAATGGCAAGGCGGGCACCCTGGACCTCAGCCT
  GACCGTCCAAGGAAAGCAGCACGTGGTGTCTGTGGAAGAAGCTCTCCTGGCAACCGGCCA
  GTGGAAGAGCATCACCCTGTTTGTGCAGGAAGACAGGGCCCAGCTGTACATCGACTGTGA
  AAAGATGGAGAATGCTGANGTGGACGTNCCCATNCCAAGCGTCTTCACCAGAGACCTGGC
  CAGCATCGCCAGACTCCGCATCGCANAGGGGGGCGTCAATGACAATTTCCAGGGGGTGCT
  GCAAAATGTGAGGGGTTGTCTTTGGACCACACCANAGNCATCCTCAGGAACAAAGGCTGN
  TNCAGCTCTACAGNGTCCTNCTCACCCTGACNAACACGTGTGAATGGTTCCAGCCTGCCA
  TCCGCACTACTACATGN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003246 unedited
  TCTGTACCGCGGCACCGCATTCTAGAGATCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTTTAAAATTCCCATATAGATTTATTGGGGGCCCTTGGCCATAAAAAAC
  AGTAATATACAACTTACACTGCTTTAAAATGGAAAAGGGATAAAAATGGGTCCAAAAAAA
  AGCCCATTCCTAAAAAAGGGTTTGGCAAATAGTAAAAAGGGGAGGGCAAAAGCAAAAAAA
  AAAAAAAAAAACCCCAACCAAAAAAAAAAAAAACCCCCCATTTCTTCAATTCAGGGGGCA
  AACTTTTTATAAAAATAAAAATCCTACCTTTACAGGCAGTTTTTCCTGATAAATTATTTA
  AATAGCATATCTCCCCAATCGGAAATTTTTTTCCCAAGGGCAAGGAAAAAATAATTTATA
  AAATTAAACCCTGGGTTTCCCAGATGATAAAAAAAAACCCTCCCTTTCAAAAATGGGATT
  TAACCTCTCAAGGCTTTTTCCTTATGGGGAAACCTTCCTCCGCAGAGTTTTTATGCTATG
  ACCAACCCGGACTTTTTAGGGCCCCCAGAAGCTTAAAGCTGGTTAAACCCTAAAACTCTT
  CCCGCTGGGCCCAAAAATCTCTTCCCCACCCCACCACAAATGGGGCAAATTTGGCCGGCA
  ACGCACCCCTCCCGGCCTCTTTGCCCCCCCTCGAACACCGAAAACAAAAAGGGCCATCCC
  CATCCCTCCCCCCGGGAAAACCCCCCAGGCGGGGGGCTGAAACACCGTCGGAACACCACC
  GGGCTAGGCTGGGAGTCGGAAACACTGGACGACGGCGCCACCGGTGGACCCCGCTCGGGG
  AACTCGGCCCCCGCGCGAAGATCAGGACCCCTTTGTCTTGTCTTCTTCGTCCACCCGGCT
  CCGTACACCAGTACATCTCCAGACACCTTCCGCGCCTCTCCCCGCTANACATCCCGCCTT
  CGGCCCCGTCCCCACGTTTCCNTCCCATCTCCCCCCCTCG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003246
• Insert Size: 3500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003246.2, NP_003237.2
• RefSeq Size: 5820 bp
• RefSeq ORF: 3513 bp
• Locus ID: 7057
• UniProt ID: P07996
• Cytogenetics: 15q14
• Domains: tsp_1, VWC, EGF_CA, TSPN, tsp_3, EGF, EGF
• Protein Families: Druggable Genome
• Protein Pathways: Bladder cancer, ECM-receptor interaction, Focal adhesion, p53 signaling pathway, TGF-beta signaling pathway
• Gene Summary: The protein encoded by this gene is a subunit of a disulfide-linked homotrimeric protein. This protein is an adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. This protein can bind to fibrinogen, fibronectin, laminin, type V collagen and integrins alpha-V/beta-1. This protein has been shown to play roles in platelet aggregation, angiogenesis, and tumorigenesis. [provided by RefSeq, Jul 2008]