TCTP (TPT1) (NM_003295) Human Untagged Clone

CAT#: SC118056

TPT1 (untagged)-Human tumor protein, translationally-controlled 1 (TPT1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TCTP
• Synonyms: HRF; p02; p23; TCTP
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_003295, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGATTATCTACCGGGACCTCATCAGCCACGATGAGATGTTCTCCGACATCTACAAGATCCGGGAGATCG
  CGGACGGGTTGTGCCTGGAGGTGGAGGGGAAGATGGTCAGTAGGACAGAAGGTAACATTGATGACTCGCT
  CATTGGTGGAAATGCCTCCGCTGAAGGCCCCGAGGGCGAAGGTACCGAAAGCACAGTAATCACTGGTGTC
  GATATTGTCATGAACCATCACCTGCAGGAAACAAGTTTCACAAAAGAAGCCTACAAGAAGTACATCAAAG
  ATTACATGAAATCAATCAAAGGGAAACTTGAAGAACAGAGACCAGAAAGAGTAAAACCTTTTATGACAGG
  GGCTGCAGAACAAATCAAGCACATCCTTGCTAATTTCAAAAACTACCAGTTCTTTATTGGTGAAAACATG
  AATCCAGATGGCATGGTTGCTCTATTGGACTACCGTGAGGATGGTGTGACCCCATATATGATTTTCTTTA
  AGGATGGTTTAGAAATGGAAAAATGTTAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003295 unedited
  GGAATTTGTAAACGACTCACTATAGGCGGCCGCGAATTCGGCACCATAGCCGCGCCCCGT
  TTCCCAGGACGAAGGGCACTCCGCACCGGACCCCGGTCCCGGCGCGCGGCGGGGCACGCG
  CCCTCCCGCGCGCGCGGGGCGCGTGGAGGGGGGGGGCGGCCCGCCGGCGGGGACAGGCGG
  GGGACCGGCTATCCGAGGCCAACCGAGGCTCCGCGGAGCTGCCGTATCGTTCCGCCTGGG
  CGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCACAGATGGTAGCTTCGCCCCATTG
  GCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCGGTTCCTCTCGTACTGAGCA
  GGATTACCATGGCAACAACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTC
  TAACCCCACTCCCCCCGAGCGCCGCTCCGGCTGCACCGCGCTCGCTCCGAGTTTCAGGCT
  CGAGCTAAGCTAGCGCCGTCGTCGTCTCCCTTCAGTCGCCATCATGATTATCTACCGGGA
  CCTCATCAGCCACGATGAGATGTTCTCCGACATCTACAAGATCCGGGAGATCGCGGACGG
  GTTGTGCCTGGAGGTGGAGGGGAAGATGGTCAGTACGACAGAACGTAACATTGATGACTC
  GCTCATTGGAGGAAATGCCTCCGCTGTATGCCCCGATGGCGAAAGTACCGAAAGCACAGT
  AATCACTGGTGTCGATATTGCCATGAACCATCACCTGCAAGGAACAAGTCTCACATAAGA
  AGCCTACAAGAAGTACATCAAAGATTACATGAAATCAATCAAAGGGAAACTCGAAGAACA
  GAGACCAGGATAGATAATACCCTTTATGACAGGGGCTGCAGAACAAATCAGCACATCCTT
  GCTAATTTCAAAACTACCAGTTCTCTATTGGTGGAAACATGAATTCCAAAGGCATGGCTG
  CTCTATGCACTACCGTGAGAATGGCGGACCCATTATGATCTTCTTAAGAAGGTTAA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003295 unedited
  GAGAAAAGGAANAANANAAAAANTATTTNNNTNTCNNTTTTACTTTGNNCCGCGGNCGCG
  CAACTAGNGATCGGTTTTTTTTTTTTTTTTTTTGAGTTTAAATGCATTTTATTTTTAGAC
  AACCTACATGACATGTTTTTCTTAAAAACAATGCCTCCACTCCAAATAAATCACAGTCAA
  AATAAATGAAGAGCTCAAGATGACATCAGTCCCATTTGTCTTAAGTCCTGGTGTTGTGTG
  GATGACAAGCAGAAGCCAGTTATGATGACAGGTGATAGATCCAAAATAATTGCCACATTT
  GTTAACATTTTTCCATTTCTAAACCATCCTTAAAGAAAATCATATATGGGGTCACACCAT
  CCTCACGGTAGTCCAATAGAGCAACCATGCCATCTGGATTCATGTTTTCACCAATAAAGA
  ACTGGTAGTTTTTGAAATTAGCAAGGATGTGCTTGATTTGTTCTGCAGCCCCTGTCATAA
  AAGGTTTTACTCTTTCTGGTCTCTGTTCTTCAAGTTTCCCTTTGATTGATTTCATGTAAT
  CTTTGATGTACTTCTTGTAGGCTTCTTTTGTGAAACTTGTTTCCTGCANGTGATGGTTCA
  TGACAATATCGACACCAGTGATTACTGTGCTTTCGGTACCTTCGCCCTCGGGGCCTTCAG
  CGGGAGCATTTCCCCAATGAGCGAGTCATCAATGTTACCTTCTGTCCTACTGACCATCTT
  NCCCCTCCACCTTCAGGCACAACCCGTCCGCGATCTCCCGGATCTTGTAGAATGTCCGAG
  ACATCTCATCGTGGCTGATGAAGTCCCCGCTAGATAATCATGATGGCCGACTGTAAGGGA
  GCACCACGACGGGCGCTTAGCTTAACACGAGCCTTGAAAACTTGGAACGAGCGCCGTGCC
  ACCCGGNACCGNGCCTCNGNGGGGAATGGGGTTAGACCCGCTCTGAAACAGGTTAAATTT
  ACCCTACTGAAGAAGGGTTGGTGGCAGGG
  
• Restriction Sites: ECoRI-NOT
• ACCN: NM_003295
• Insert Size: 1340 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003295.1, NP_003286.1
• RefSeq Size: 830 bp
• RefSeq ORF: 519 bp
• Locus ID: 7178
• UniProt ID: P13693
• Cytogenetics: 13q14.13
• Domains: TCTP
• Gene Summary: This gene encodes a protein that is a regulator of cellular growth and proliferation. Its mRNA is highly structured and contains an oligopyrimidine tract (5'-TOP) in its 5' untranslated region that functions to repress its translation under quiescent conditions. The encoded protein is involved in a variety of cellular pathways, including apoptosis, protein synthesis and cell division. It binds to and stabilizes microtubules, and removal of this protein through phosphorylation is required for progression through mitotic and meiotic cell divisions. This gene is known to play a role in carcinogenesis, and is upregulated in some cancer cells. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2017]
  Transcript Variant: This variant (2) uses an alternate splice site in the 3' coding region, which results in a frameshift, compared to variant 1. The encoded isoform (2) has a shorter C-terminus than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.