Semaphorin 7a (SEMA7A) (NM_003612) Human Untagged Clone

CAT#: SC117884

SEMA7A (untagged)-Human semaphorin 7A, GPI membrane anchor (John Milton Hagen blood group) (SEMA7A), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Semaphorin 7a
• Synonyms: CD108; CDw108; H-SEMA-K1; H-Sema-L; JMH; SEMAK1; SEMAL
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC117884 sequence for NM_003612 edited (data generated by NextGen Sequencing)
  ATGACGCCTCCTCCGCCCGGACGTGCCGCCCCCAGCGCACCGCGCGCCCGCGTCCCTGGC
  CCGCCGGCTCGGTTGGGGCTTCCGCTGCGGCTGCGGCTGCTGCTGCTGCTCTGGGCGGCC
  GCCGCCTCCGCCCAGGGCCACCTAAGGAGCGGACCCCGCATCTTCGCCGTCTGGAAAGGC
  CATGTAGGGCAGGACCGGGTGGACTTTGGCCAGACTGAGCCGCACACGGTGCTTTTCCAC
  GAGCCAGGCAGCTCCTCTGTGTGGGTGGGAGGACGTGGCAAGGTCTACCTCTTTGACTTC
  CCCGAGGGCAAGAACGCATCTGTGCGCACGGTGAATATCGGCTCCACAAAGGGGTCCTGT
  CTGGATAAGCGGGACTGCGAGAACTACATCACTCTCCTGGAGAGGCGGAGTGAGGGGCTG
  CTGGCCTGTGGCACCAACGCCCGGCACCCCAGCTGCTGGAACCTGGTGAATGGCACTGTG
  GTGCCACTTGGCGAGATGAGAGGCTACGCCCCCTTCAGCCCGGACGAGAACTCCCTGGTT
  CTGTTTGAAGGGGACGAGGTGTATTCCACCATCCGGAAGCAGGAATACAATGGGAAGATC
  CCTCGGTTCCGCCGCATCCGGGGCGAGAGTGAGCTGTACACCAGTGATACTGTCATGCAG
  AACCCACAGTTCATCAAAGCCACCATCGTGCACCAAGACCAGGCTTACGATGACAAGATC
  TACTACTTCTTCCGAGAGGACAATCCTGACAAGAATCCTGAGGCTCCTCTCAATGTGTCC
  CGTGTGGCCCAGTTGTGCAGGGGGGACCAGGGTGGGGAAAGTTCACTGTCAGTCTCCAAG
  TGGAACACTTTTCTGAAAGCCATGCTGGTATGCAGTGATGCTGCCACCAACAAGAACTTC
  AACAGGCTGCAAGACGTCTTCCTGCTCCCTGACCCCAGCGGCCAGTGGAGGGACACCAGG
  GTCTATGGTGTTTTCTCCAACCCCTGGAACTACTCAGCCGTCTGTGTGTATTCCCTCGGT
  GACATTGACAAGGTCTTCCGTACCTCCTCACTCAAGGGCTACCACTCAAGCCTTCCCAAC
  CCGCGGCCTGGCAAGTGCCTCCCAGACCAGCAGCCGATACCCACAGAGACCTTCCAGGTG
  GCTGACCGTCACCCAGAGGTGGCGCAGAGGGTGGAGCCCATGGGGCCTCTGAAGACGCCA
  TTGTTCCACTCTAAATACCACTACCAGAAAGTGGCCGTCCACCGCATGCAAGCCAGCCAC
  GGGGAGACCTTTCATGTGCTTTACCTAACTACAGACAGGGGCACTATCCACAAGGTGGTG
  GAACCGGGGGAGCAGGAGCACAGCTTCGCCTTCAACATCATGGAGATCCAGCCCTTCCGC
  CGCGCGGCTGCCATCCAGACCATGTCGCTGGATGCTGAGCGGAGGAAGCTGTATGTGAGC
  TCCCAGTGGGAGGTGAGCCAGGTGCCCCTGGACCTGTGTGAGGTCTATGGCGGGGGCTGC
  CACGGTTGCCTCATGTCCCGAGACCCCTACTGCGGCTGGGACCAAGGCCGCTGCATCTCC
  ATCTACAGCTCCGAACGGTCAGTGCTGCAATCCATTAATCCAGCCGAGCCACACAAGGAG
  TGTCCCAACCCCAAACCAGACAAGGCCCCACTGCAGAAGGTTTCCCTGGCCCCAAACTCT
  CGCTACTACCTGAGCTGCCCCATGGAATCCCGCCACGCCACCTACTCATGGCGCCACAAG
  GAGAACGTGGAGCAGAGCTGCGAACCTGGTCACCAGAGCCCCAACTGCATCCTGTTCATC
  GAGAACCTCACGGCGCAGCAGTACGGCCACTACTTCTGCGAGGCCCAGGAGGGCTCCTAC
  TTCCGCGAGGCTCAGCACTGGCAGCTGCTGCCCGAGGACGGCATCATGGCCGAGCACCTG
  CTGGGTCATGCCTGTGCCCTGGCCGCCTCCCTCTGGCTGGGGGTGCTGCCCACACTCACT
  CTTGGCTTGCTGGTCCACTAG
  
  Clone variation with respect to NM_003612.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003612 unedited
  CCCCCCCCCGNNNNNNNNNNNCNNNCNCNNNNCCCCNCCCCCCCCNNNGGGTTCATAATT
  GNATACAATCATATAGGCGGCCGCGAAATTCGCACGAGTCGCTCCCGGGGCCACGGGNAG
  ACGCCTCCTCCGCCCGGACGTGCCGCCCCCAGCGCACCGCGCGCCCGCGTCCCTGGCCCG
  CCGGCTCGGTTGGGGCTTCCGCTGCGGCTGCGGCTGCTGCTGCTGCTCTGGGCGGCCGCC
  GCCTCCGCCCAGGGCCACCTAAGGAGCGGACCCCGCATCTTCGCCGTCTGGAAAGGCCAT
  GTAGGGCAGGACCGGGTGGACTTTGGCCAGACTGAGCCGCACACGGTGCTTTTCCACGAG
  CCAGGCAGCTCCTCTGTGTGGGTGGGAGGACGTGGCAAGGTCTACCTCTTTGACTTCCCC
  GAGGGCAAGAACGCATCTGTGCGCACGGTGAATATCGGCTCCACAAAGGGGTCCTGTCTG
  GATAAGCGGGACTGCGAGAACTACATCACTCTCCTGGAGAGGCGGAGTGAGGGGCTGCTG
  GCCTGTGGCACCAACGCCCGGCACCCCAGCTGCTGGAACCTGGTGAATGGCACTGTGGTG
  CCACTTGGCGAGATGAGAGGCTACGCCCCCTTCAGCCCGGACGAGAACTCCCTGGTTCTG
  TTTGAAGGGGACGAGGTGTATTCCACCATCCNGAAGCAGGAATACAATGGGAAGATCCCT
  NCGTTCCGCCGCATCCGGNGCGAGAGTGAGCTGTACACCAGTGATACTGTCATGCAGAAC
  CCACAGTTCATCAAAGCCACCATCGTGCACCAAGACCCAGCTTNACGATGACAGATCTAC
  TACTTCTTNCGAGAGGACATCCTGACAAGAATCCTGAGGCCTCTCTCATGTGTCCCGTGT
  TGCCCCAGTGTGCCAGGGGGACCAGGTTGGGAAAGTCACTGTCAGTCTCCAGTGGGACAC
  TTTTCTGGAGAN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003612 unedited
  CCGTGGCGCCATTGGGCGATGGTCAACTTGCCAGGGCCAGGAAAGCACTGGGGAAGGGTC
  ACAGGGCTGCCACCCGGGTTCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAG
  TCGAGTTTTTTTTTTTTTTTTTTTAAGCCTAACATAAAAATAGAGCTTTTTCCGTCCTTC
  CGTCCGGAAAGCAAACATCCTTCAATAAACATGCAAGGCGGCTGTCCTGTGGGACCCAGG
  ACCAGAGAGGGAGCTGCAGAGGACAGGGCTGGACAGAGGGTAGCCCTGGGTCTTCAAGAA
  CACCAGCCACCCAGCCATGAGAGAGGGAGGGGAAGGAGGCAATGTGGGTACCAAGAGTCC
  AGAAGGACTCAGGCCTCAGCCCCAGGGTCGAGATGGAGTCCCAGCTCTCCTATCCAAACC
  CACTCCCCGACCCATGGGCTCTTGGGCTGGGAGCATCGCTGCATTTAGTCAAGTTTGAGG
  AGTCTGAAAAATATTTTCCAGAAGATAAAGTCTTGGGTCATCGATGCCCCAGCTTCACAG
  TCGGTGCCCTCATTCTCAGCCCCTCACCATCCGTGCGCCACCTGGGGCCCAGCAGCCGCC
  TGCGGCTGGACGTCTCCAGGCCTGGCATCCTCCACTGGGTTATTCTGTCCCCTGGAAGAA
  GTGGCAGGCAAGGAGCTCCCGGGCCAGCCGGCTCTGAGTGTGAGACGTTCTAGTGCCCTG
  GGCTGCANAAGCCTGAGGCATGCCCAGCCTCGGGAGGCCCTAGTGGACCAGCAAGCCAAG
  AGTGAGTGTGGGCAGCACCCCCAGCCAGAGGGAGGCGGCCAGGGCACAGGCATGACCCAG
  CAGGTGCTCGGCCATGATGCCGTCCTCGGGCAGCAGCTGCCAGTGCTGAGCCTCGCAGAA
  GTAAGAGCCCT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003612
• Insert Size: 2900 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003612.1, NP_003603.1
• RefSeq Size: 2661 bp
• RefSeq ORF: 2001 bp
• Locus ID: 8482
• UniProt ID: O75326
• Cytogenetics: 15q24.1
• Domains: Sema, PSI
• Protein Pathways: Axon guidance
• Gene Summary: This gene encodes a member of the semaphorin family of proteins. The encoded preproprotein is proteolytically processed to generate the mature glycosylphosphatidylinositol (GPI)-anchored membrane glycoprotein. The encoded protein is found on activated lymphocytes and erythrocytes and may be involved in immunomodulatory and neuronal processes. The encoded protein carries the John Milton Hagen (JMH) blood group antigens. Mutations in this gene may be associated with reduced bone mineral density (BMD). Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. [provided by RefSeq, Feb 2016]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record represents the SEMA7A*001.1.1 allele.