kynurenine 3 monooxygenase (KMO) (NM_003679) Human Untagged Clone

CAT#: SC117858

KMO (untagged)-Human kynurenine 3-monooxygenase (kynurenine 3-hydroxylase) (KMO)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: kynurenine 3 monooxygenase
• Synonyms: dJ317G22.1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC117858 sequence for NM_003679 edited (data generated by NextGen Sequencing)
  ATGGACTCATCTGTCATTCAAAGGAAAAAAGTAGCTGTCATTGGTGGTGGCTTGGTTGGC
  TCATTACAAGCATGCTTTCTTGCAAAGAGGAATTTCCAGATTGATGTATATGAAGCTAGG
  GAAGATACTCGAGTGGCTACCTTCACACGTGGAAGAAGCATTAACTTAGCCCTTTCTCAT
  AGAGGACGACAAGCCTTGAAAGCTGTTGGCCTGGAAGATCAGATTGTATCCCAAGGTATT
  CCCATGAGAGCAAGAATGATCCACTCTCTTTCAGGAAAAAAGTCTGCAATTCCCTATGGG
  ACAAAGTCTCAGTATATTCTTTCTGTAAGCAGAGAAAATCTAAACAAGGATCTATTGACT
  GCTGCTGAGAAATACCCCAATGTGAAAATGCACTTTAACCACAGGCTGTTGAAATGTAAT
  CCAGAGGAAGGAATGATCACAGTGCTTGGATCTGACAAAGTTCCCAAAGATGTCACTTGT
  GACCTCATTGTAGGATGTGATGGAGCCTATTCAACTGTCAGATCTCACCTGATGAAGAAA
  CCTCGCTTTGATTACAGTCAGCAGTACATTCCTCATGGGTACATGGAGTTGACTATTCCA
  CCTAAGAACGGAGATTATGCCATGGAACCTAATTATCTGCATATTTGGCCTAGAAATACC
  TTTATGATGATTGCACTTCCTAACATGAACAAATCATTCACATGTACTTTGTTCATGCCC
  TTTGAAGAGTTTGAAAAACTTCTAACCAGTAATGATGTGGTAGATTTCTTCCAGAAATAC
  TTTCCAGATGCCATCCCTCTAATTGGAGAGAAACTCCTAGTGCAAGATTTCTTCCTGTTG
  CCTGCCCAGCCCATGATATCTGTAAAGTGCTCTTCATTTCACTTTAAATCTCACTGTGTA
  CTGCTGGGAGATGCAGCTCATGCTATAGTGCCGTTTTTTGGGCAAGGAATGAATGCGGGC
  TTTGAAGACTGCTTGGTATTTGATGAGTTAATGGATAAATTCAGTAACGACCTTAGTTTG
  TGTCTTCCTGTGTTCTCAAGATTGAGAATCCCAGATGATCACGCGATTTCAGACCTATCC
  ATGTACAATTACATAGAGATGCGAGCACATGTCAACTCAAGCTGGTTCATTTTTCAGAAG
  AACATGGAGAGATTTCTTCATGCGATTATGCCATCGACCTTTATCCCTCTCTATACAATG
  GTCACTTTTTCCAGAATAAGATACCATGAGGCTGTGCAGCGTTGGCATTGGCAAAAAAAG
  GTGATAAACAAAGGACTCTTTTTCTTGGGATCACTGATAGCCATCAGCAGTACCTACCTA
  CTTATACACTACATGTCACCACGATCTTTCCTCTGCTTGAGAAGACCATGGAACTGGATA
  GCTCACTTCCGGAATACAACATGTTTCCCCGCAAAGGCCGTGGACTCCCTAGAACAAATT
  TCCAATCTCATTAGCAGGTGA
  
  Clone variation with respect to NM_003679.3
  786 g=>a;1354 c=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003679 unedited
  NAACGTTCAAATTTGTAAACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGCACTCA
  GTGACAGAAGCAACAATAATTGTGAAAAAACTTCAGCAGTTATGGACTCATCTGTCATTC
  AAAGGAAAAAAGTAGCTGTCATTGGTGGTGGCTTGGTTGGCTCATTACAAGCATGCTTTC
  TTGCAAAGAGGAATTTCCAGATTGATGTATATGAAGCTAGGGAAGATACTCGAGTGGCTA
  CCTTCACACGTGGAAGAAGCATTAACTTAGCCCTTTCTCATAGAGGACGACAAGCCTTGA
  AAGCTGTTGGCCTGGAAGATCAGATTGTATCCCAAGGTATTCCCATGAGAGCAAGAATGA
  TCCACTCTCTTTCAGGAAAAAAGTCTGCAATTCCCTATGGGACAAAGTCTCAGTATATTC
  TTTCTGTAAGCAGAGAAAATCTAAACAAGGATCTATTGACTGCTGCTGAGAAATACCCCA
  ATGTGAAAATGCACTTTAACCACAGGCTGTTGAAATGTAATCCAGAGGAAGGAATGATCA
  CAGTGCTTGGATCTGACAAAGTTCCCAAAGATGTCACTTGTGACCTCATTGTAGGATGTG
  ATGGAGCCTATTCAACTGTCAGATCTCACCTGATGAAGAAACCTCGCTTTGATTACAGTC
  AGCAGTACATTCCTCATGGGTACATGGAGTTGACTATTCCCACCTAGAACGAGATTATGC
  CATGGGACCTAATTATCTGCATATTTGGCCTAGAAATACCTTTATGATGATTGCACTTNC
  CTACATGAAACAATCATTCACATGTACTTTGTTCATGCCCTTTTGAAGAGTTTGAAAAAC
  TCTNACCAGTAATGATGTGGTAGATTTNCTTCCAGAATACTTTCCAGATGCCATCCCNTC
  TATTGNAGAGAAACTNNCTAGTGCAGAC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003679 unedited
  GCGGCCCGCAATCTAGNATCGAGTTTTTTTTTTTTTTTTTTTGCCCAATTTTTATTTCTT
  ATATGACTCTAGTGTTTCATCTTCATAATTAATCATGTTTGAAGGATTTCTGAGTGACTC
  AGCAGCCTGTTAAAGAAGGATGAACCAAAGAAAACATTTCACTAAATGTGCTTTTAAAAA
  TCAAGTGTATTGCTGGTTCTGCTGCAGTATGTAGTCGAAGAATAAATTAGTAAATTGCTT
  CTGAGGGTCTGAAATTGAATAAAGTAATGGCTTTGTATTTCTATAAAAGTTGTCTCCCCT
  TGTTTCCTTTCCATTCTGGCACATGTAGACATTTAATTTATGAATAAAACCCTTTCCTGT
  CCTTCACTTAGTATGATATTAATAGCCTTTTTCTTATCTTTAGAGGGTAACTCTGGGCTG
  GCAGCGGTGGCTCACAACTCTAATCCCTGTACTTTGGGAGGCTGAGGTGGGCAGATCACA
  TGAGGCCAGGAGTTTGAGACTGGCCTGNGCAGCATGGCAAGACCCGGTCTCTACTGAAAA
  ATACCATGGTAGCCCCAGCTATTTGNGAGGCTGAAGTGGGAGGATTGCTTGAGCCCAGGA
  GGCAGAGGTTGCAGTGAACCCAGATCTCACCACTGCACTCCAGCCTGGTCAACAGAGTGA
  GACCTGATCTCTAATACATAANTAAATAGAGATAGATAGATAGATAGATAGATAGATAGA
  TAGATAGATGATCGATAGATAGATAGATGATAGATAGATAGACGATAAACCAGATGATGA
  TACTACCTATATTTGAAGGTGGGAAATAAAATGAGGTCATTCTGAAGTCTCCTGGACTCC
  CCTGGAATCCTTATTTCCATTTCTGGTTTTAACTAGAAAGTCTGAACCCTGGGGNTATGA
  AGTTTCCATCACNGTATGGAAAGGGCAGTTACATGCTAGTAAGTTGGGATTTTAGCCCTA
  GGGTG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003679
• Insert Size: 1461 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003679.2, NP_003670.1
• RefSeq Size: 4992 bp
• RefSeq ORF: 1461 bp
• Locus ID: 8564
• UniProt ID: O15229
• Cytogenetics: 1q43
• Domains: Monooxygenase
• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Tryptophan metabolism
• Gene Summary: This gene encodes a mitochondrion outer membrane protein that catalyzes the hydroxylation of L-tryptophan metabolite, L-kynurenine, to form L-3-hydroxykynurenine. Studies in yeast identified this gene as a therapeutic target for Huntington disease. [provided by RefSeq, Oct 2011]