IQGAP1 (NM_003870) Human Untagged Clone

CAT#: SC117725

IQGAP1 (untagged)-Human IQ motif containing GTPase activating protein 1 (IQGAP1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: IQGAP1
• Synonyms: HUMORFA01; p195; SAR1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_003870 edited
  GAATTCGGCACGAGCTCAGCAGAGACTCGGGCTCGTCCGCCATGTCCGCCGCAGACGAGG
  TTGACGGGCTGGGCGTGGCCCGGCCGCACTATGGCTCTGTCCTGGATAATGAAAGACTTA
  CTGCAGAGGAGATGGATGAAAGGAGACGTCAGAACGTGGCTTATGAGTACCTTTGTCATT
  TGGAAGAAGCGAAGAGGTGGATGGAAGCATGCCTAGGGGAAGATCTGCCTCCCACCACAG
  AACTGGAGGAGGGGCTTAGGAATGGGGTCTACCTTGCCAAACTGGGGAACTTCTTCTCTC
  CCAAAGTAGTGTCCCTGAAAAAAATCTATGATCGAGAACAGACCAGATACAAGGCGACTG
  GCCTCCACTTTAGACACACTGATAATGTGATTCAGTGGTTGAATGCCATGGATGAGATTG
  GATTGCCTAAGATTTTTTACCCAGAAACTACAGATATCTATGATCGAAAGAACATGCCAA
  GATGTATCTACTGTATCCATGCACTCAGTTTGTACCTGTTCAAGCTAGGCCTGGCCCCTC
  AGATTCAAGACCTATATGGAAAGGTTGACTTCACAGAAGAAGAAATCAACAACATGAAGA
  CTGAGTTGGAGAAGTATGGCATCCAGATGCCTGCCTTTAGCAAGATTGGGGGCATCTTGG
  CTAATGAACTGTCAGTGGATGAAGCCGCATTACATGCTGCTGTTATTGCTATTAATGAAG
  CTATTGACCGTAGAATTCCAGCCGACACATTTGCAGCTTTGAAAAATCCGAATGCCATGC
  TTGTAAATCTTGAAGAGCCCTTGGCATCCACTTACCAGGATATACTTTACCAGGCTAAGC
  AGGACAAAATGACAAATGCTAAAAACAGGACAGAAAACTCAGAGAGAGAAAGAGATGTTT
  ATGAGGAGCTGCTCACGCAAGCTGAAATTCAAGGCAATATAAACAAAGTCAATACATTTT
  CTGCATTAGCAAATATCGACCTGGCTTTAGAACAAGGAGATGCACTGGCCTTGTTCAGGG
  CTCTGCAGTCACCAGCCCTGGGGCTTCGAGGACTGCAGCAACAGAATAGCGACTGGTACT
  TGAAGCAGCTCCTGAGTGATAAACAGCAGAAGAGACAGAGTGGTCAGACTGACCCCCTGC
  AGAAGGAGGAGCTGCAGTCTGGAGTGGATGCTGCAAACAGTGCTGCCCAGCAATATCAGA
  GAAGATTGGCAGCAGTAGCACTGATTAATGCTGCAATCCAGAAGGGTGTTGCTGAGAAGA
  CTGTTTTGGAACTGATGAATCCCGAAGCCCAGCTGCCCCAGGTGTATCCATTTGCCGCCG
  ATCTCTATCAGAAGGAGCTGGCTACCCTGCAGCGACAAAGTCCTGAACATAATCTCACCC
  ACCCAGAGCTCTCTGTCGCAGTGGAGATGTTGTCATCGGTGGCCCTGATCAACAGGGCAT
  TGGAATCAGGAGATGTGAATACAGTGTGGAAGCAATTGAGCAGTTCAGTTACTGGTCTTA
  CCAATATTGAGGAAGAAAACTGTCAGAGGTATCTCGATGAGTTGATGAAACTGAAGGCTC
  AGGCACATGCAGAGAATAATGAATTCATTACATGGAATGATATCCAAGCTTGCGTGGACC
  ATGTGAACCTGGTGGTGCAAGAGGAACATGAGAGGATTTTAGCCATTGGTTTAATTAATG
  AAGCCCTGGATGAAGGTGATGCCCAAAAGACTCTGCAGGCCCTACAGATTCCTGCAGCTA
  AACTTGAGGGAGTCCTTGCAGAAGTGGCCCAGCATTACCAAGACACGCTGATTAGAGCGA
  AGAGAGAGAAAGCCCAGGAAATCCAGGATGAGTCAGCTGTGTTATGGTTGGATGAAATTC
  AAGGTGGAATCTGGCAGTCCAACAAAGACACCCAAGAAGCACAGAAGTTTGCCTTAGGAA
  TCTTTGCCATTAATGAGGCAGTAGAAAGTGGTGATGTTGGCAAAACACTGAGTGCCCTTC
  GCTCCCCTGATGTTGGCTTGTATGGAGTCATCCCTGAGTGTGGTGAAACTTACCACAGTG
  ATCTTGCTGAAGCCAAGAAGAAAAAACTGGCAGTAGGAGATAATAACAGCAAGTGGGTGA
  AGCACTGGGTAAAAGGTGGATATTATTATTACCACAATCTGGAGACCCAGGAAGGAGGAT
  GGGATGAACCCCCAAATTTTGTGCAAAATTCTATGCAGCTTTCTCGGGAGGAGATCCAGA
  GTTCTATCTCTGGGGTGACTGCCGCATATAACCGAGAACAGCTGTGGCTGGCCAATGAAG
  GCCTGATCACCAGGCTGCAGGCTCGCTGCCGTGGATACTTAGTTCGACAGGAATTCCGAT
  CCAGGATGAATTTCCTGAAGAAACAAATCCCTGCCATCACCTGCATTCAGTCACAGTGGA
  GAGGATACAAGCAGAAGAAGGCATATCAAGATCGGTTAGCTTACCTGCGCTCCCACAAAG
  ATGAAGTTGTAAAGATTCAGTCCCTGGCAAGGATGCACCAAGCTCGAAAGCGCTATCGAG
  ATCGCCTGCAGTACTTCCGGGACCATATAAATGACATTATCAAAATCCAGGCTTTTATTC
  GGGCAAACAAAGCTCGGGATGACTACAAGACTCTCATCAATGCTGAGGATCCTCCTATGG
  TTGTGGTCCGAAAATTTGTCCACCTGCTGGACCAAAGTGACCAGGATTTTCAGGAGGAGC
  TTGACCTTATGAAGATGCGGGAAGAGGTTATCACCCTCATTCGTTCTAACCAGCAGCTGG
  AGAATGACCTCAATCTCATGGATATCAAAATTGGACTGCTAGTGAAAAATAAGATTACGT
  TGCAGGATGTGGTTTCCCACAGTAAAAAACTTACCAAAAAAAATAAGGAACAGTTGTCTG
  ATATGATGATGATAAATAAACAGAAGGGAGGTCTCAAGGCTTTGAGCAAGGAGAAGAGAG
  AGAAGTTGGAAGCTTACCAGCACCTGTTTTATTTATTGCAAACCAATCCCACCTATCTGG
  CCAAGCTCATTTTTCAGATGCCCCAGAACAAGTCCACCAAGTTCATGGACTCTGTAATCT
  TCACACTCTACAACTACGCGTCCAACCAGCGAGAGGAGTACCTGCTCCTGCGGCTCTTTA
  AGACAGCACTCCAAGAGGAAATCAAGTCGAAGGTAGATCAGATTCAAGAGATTGTGACAG
  GAAATCCTACGGTTATTAAAATGGTTGTAAGTTTCAACCGTGGTGCCCGTGGCCAGAATG
  CCCTGAGACAGATCTTGGCCCCAGTCGTGAAGGAAATTATGGATGACAAATCTCTCAACA
  TCAAAACTGACCCTGTGGATATTTACAAATCTTGGGTTAATCAGATGGAGTCTCAGACAG
  GAGAGGCAAGCAAACTGCCCTATGATGTGACCCCTGAGCAGGCGCTAGCTCATGAAGAAG
  TGAAGACACGGCTAGACAGCTCCATCAGGAACATGCGGGCTGTGACAGACAAGTTTCTCT
  CAGCCATTGTCAGCTCTGTGGACAAAATCCCTTATGGGATGCGCTTCATTGCCAAAGTGC
  TGAAGGACTCGTTGCATGAGAAGTTCCCTGATGCTGGTGAGGATGAGCTGCTGAAGATTA
  TTGGTAACTTGCTTTATTATCGATACATGAATCCAGCCATTGTTGCTCCTGATGCCTTTG
  ACATCATTGACCTGTCAGCAGGAGGCCAGCTTACCACAGACCAACGCCGAAATCTGGGCT
  CCATTGCAAAAATGCTTCAGCATGCTGCTTCCAATAAGATGTTTCTGGGAGATAATGCCC
  ACTTAAGCATCATTAATGAATATCTTTCCCAGTCCTACCAGAAATTCAGACGGTTTTTCC
  AAACTGCTTGTGATGTCCCAGAGCTTCAGGATAAATTTAATGTGGATGAGTACTCTGATT
  TAGTAACCCTCACCAAACCAGTAATCTACATTTCCATTGGTGAAATCATCAACACCCACA
  CTCTCCTGTTGGATCACCAGGATGCCATTGCTCCGGAGCACAATGATCCAATCCACGAAC
  TGCTGGACGACCTCGGCGAGGTGCCCACCATCGAGTCCCTGATAGGGGAAAGCTCTGGCA
  ATTTAAATGACCCAAATAAGGAGGCACTGGCTAAGACGGAAGTGTCTCTCACCCTGACCA
  ACAAGTTCGACGTGCCTGGAGATGAGAATGCAGAAATGGATGCTCGAACCATCTTACTGA
  ATACAAAACGTTTAATTGTGGATGTCATCCGGTTCCAGCCAGGAGAGACCTTGACTGAAA
  TCCTAGAAACACCAGCCACCAGTGAACAGGAAGCAGAACATCAGAGAGCCATGCAGAGAC
  GTGCTATCCGTGATGCCAAAACACCTGACAAGATGAAAAAGTCAAAATCTGTAAAGGAAG
  ACAGCAACCTCACTCTTCAAGAGAAGAAAGAGAAGATCCAGACAGGTTTAAAGAAGCTAA
  CAGAGCTTGGAACCGTGGACCCAAAGAACAAATACCAGGAACTGATCAACGACATTGCCA
  GGGATATTCGGAATCAGCGGAGGTACCGACAGAGGAGAAAGGCCGAACTAGTGAAACTGC
  AACAGACATACGCTGCTCTGAACTCTAAGGCCACCTTTTATGGGGAGCAGGTGGATTACT
  ATAAAAGCTATATCAAAACCTGCTTGGATAACTTAGCCAGCAAGGGCAAAGTCTCCAAAA
  AGCCTAGGGAAATGAAAGGAAAGAAAAGCAAAAAGATTTCTCTGAAATATACAGCAGCAA
  GACTACATGAAAAAGGAGTTCTTCTGGAAATTGAGGACCTGCAAGTGAATCAGTTTAAAA
  ATGTTATATTTGAAATCAGTCCAACAGAAGAAGTTGGAGACTTCGAAGTGAAAGCCAAAT
  TCATGGGAGTTCAAATGGAGACTTTTATGTTACATTATCAGGACCTGCTGCAGCTACAGT
  ATGAAGGAGTTGCAGTCATGAAATTATTTGATAGAGCTAAAGTAAATGTCAACCTCCTGA
  TCTTCCTTCTCAACAAAAAGTTCTACGGGAAGTAATTGATCGTTTGCTGCCAGCCCAGAA
  GXXXXXXXXXXXXXXXXXXXXXXXXXXTAAAGTATAGATGTATGTCTTAAAATGTGGGTG
  ATAGGAATTTTAAAGATTTATATAATGCATCAAAAGCCTTAGAATAAGAAAAGCTTTTTT
  TAAATTGCTTTATCTGTATATCTGAACTCTTGAAACTTATAGCTAAAACAATAGGATTTA
  TCTGCAGTGTTGCAGGGAGATAATTCTGCCTTAAATTGTCTAAAACAAAAACAAAACCAG
  CCAACCTATGTTACACGTGAGATTAAAACCAATTTTTTCCCCATTTTTTTTCCTTTTTTC
  TCTTGCTGCCCACATTGTGCCTTTATTTTATGAGCCCCAGTTTTTTGGGCTTAGTTTAAA
  AAAAAAATCAAGTCTAAACATTGCATTTAGAAAGCTTTTGTTCTTGGATAAAAAGTCATA
  CACTTTAAAAAAAAAAAAAACTTTTTCCAGGAAAATATATTGAAATCATGCTGCTGAGCC
  TCTATTTTCTTTCTTTGATGTTTTGATTCAGTATTCTTTTATCATAAATTTTTAGCATTT
  AAAAATTCACTGATGTACATTAAGCCAATAAACTGCTTTAATGAATAACAAAAAAAAAAA
  AAAAAAAACTCGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003870 unedited
  TTCAAATTATGTAATACGACTCACTATAGGGCGGCCCGCGATTCGGCACGAGCTCAGCAG
  AGACTCGGGCTCGTCCGCCATGTCCGCCGCAGACGAGGTTGACGGGCTGGGCGTGGCCCG
  GCCGCACTATGGCTCTGTCCTGGATAATGAAAGACTTACTGCAGAGGAGATGGATGAAAG
  GAGACGTCAGAACGTGGCTTATGAGTACCTTTGTCATTTGGAAGAAGCGAAGAGGTGGAT
  GGAAGCATGCCTAGGGGAAGATCTGCCTCCCACCACAGAACTGGAGGAGGGGCTTAGGAA
  TGGGGTCTACCTTGCCAAACTGGGGAACTTCTTCTCTCCCAAAGTAGTGTCCCTGAAAAA
  AATCTATGATCGAGAACAGACCAGATACAAGGCGACTGGCCTCCACTTTAGACACACTGA
  TAATGTGATTCAGTGGTTGAATGCCATGGATGAGATTGGATTGCCTAAGATTTTTTACCC
  AGAAACTACAGATATCTATGATCGAAAGAACATGCCAAGATGTATCTACTGTATCCATGC
  ACTCAGTTTGTACCTGTTCAAGCTAGGCCTGGCCCCTCAGATTCAAGACCTATATGGAAA
  GGTTGACTTCACAGAAGAAGAAATCAACAACATGAAGACTGAGTTGGAGAAGTATGGCAT
  CCAGATGCCTGCCTTTAGCAAGATTGGGGGCATCTTGGCTAATGAACTGTCAGTGGATGA
  AGCCGCATTACATGCTGCTGTTATTGCTATTAATGAAGCTATTGACCGTAGAATTCCAGC
  CGACACATTTGCAGCTTTGAAAAATCCGAATGCCATGCTTGNTAATCTTGAAGAGCCCTT
  GGCATNCACTTACCAGNATATACTNNTACCAGNNCTAGCAGACANNATGACAATGCTAAA
  ACAGGACAGAAACTCAGAGAGAGAAAGAGAGTTNATGAGGAGCTGCTCCGC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003870 unedited
  CTGCAATTACTTGCACCCGGCCGCAATCTACGAGCGACTTTTTTTTTTTTTTTTTTTGTT
  ATTCATTAAAGCAGTTTATTGGCTTACAGTACATCAGTGAATTTTTAAATGCTAAAAATT
  TATGATAAAAGAATACTGAATCAAAACATCAAAGAAAGAAAATAGAGGCTCAGCAGCATG
  ATTTCAATATATTTTCCTGGAAAAAGTTTTTTTTTTTTTTAAAGTGTATGACTTTTTATC
  CAAGAACAAAAGCTTTCTAAATGCAATGTTTAGACTTGATTTTTTTTTTAAACTAAGCCC
  AGAAAACTGGGGCTCATAAAATAAAGGCACAATGTGGGCAGCAAGACAAAAAAGGAGAAA
  AAATGGGGAAAAAATTGGCTTTAATCTCACGTGTAACATAGGTTGGCTGGTTTTGTTTTT
  GTTTTAGACAATTTAAGGCAGAATTATCTCCCTGCAACACTGCAGATAAATCCTAGTGTT
  TTAGCTATAAGTTTCAAGAGTTCAGATATACAGATAAAGCAATTTAAAAAAAGCTTTTCT
  TATTCTAAGGCTTTTGATGCATTATATAAATTTTTAAAATTCCTATCACCCACATTTTAA
  GACCTACATCTATACTTTAATAAGGCACTCTTAAGACATAAAACCGCTTTAAAAACAATG
  ACATTCTACACTAGGGCTAACCCATTCCTCTGCCCTAGGGATGGAGTGAACTGCCTCTCT
  GAATAAGCCACACTTCTGTGCACCAACACCAACCGGCCGGTGCCCTGACCCACTCTGAAC
  TTACCTGATCACCTTCACTTTAACACTGGGCCTCTCCCAACCCTACCCGGGGAGCTCCCC
  CACCCCAACTCCCCTATTTGACAATGAACACCACCCCCTTCTTTCTTTTTTCTAAACACG
  TTTCCTG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003870
• Insert Size: 6500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003870.3, NP_003861.1
• RefSeq Size: 7219 bp
• RefSeq ORF: 4974 bp
• Locus ID: 8826
• UniProt ID: P46940
• Cytogenetics: 15q26.1
• Domains: IQ, RasGAP_C, WW, CH, RasGAP
• Protein Families: Druggable Genome
• Protein Pathways: Adherens junction, Regulation of actin cytoskeleton
• Gene Summary: This gene encodes a member of the IQGAP family. The protein contains four IQ domains, one calponin homology domain, one Ras-GAP domain and one WW domain. It interacts with components of the cytoskeleton, with cell adhesion molecules, and with several signaling molecules to regulate cell morphology and motility. Expression of the protein is upregulated by gene amplification in two gastric cancer cell lines. [provided by RefSeq, Jul 2008]