Natriuretic Peptide Receptor B (NPR2) (NM_003995) Human Untagged Clone

CAT#: SC117646

NPR2 (untagged)-Human natriuretic peptide receptor B/guanylate cyclase B (atrionatriuretic peptide receptor B) (NPR2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Natriuretic Peptide Receptor B
• Synonyms: AMDM; ANPb; ANPRB; ECDM; GC-B; GCB; GUC2B; GUCY2B; NPRB; NPRBi; SNSK
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_003995 edited
  GAATTCGGCACGAGCCCAAGTTCTGGGGGCGGTGGGGCTGCTGCTTTATCCCCATGGCGC
  TGCCATCACTCCTGCTGTTGGTGGCAGCCCTGGCAGGTGGGGTGCGTCCTCCCGGGGCGC
  GGAACCTGACGCTGGCGGTGGTGCTGCCAGAACACAACCTGAGCTATGCCTGGGCCTGGC
  CACGGGTGGGACCCGCTGTGGCACTAGCTGTGGAGGCTCTGGGCCGGGCACTGCCCGTGG
  ACCTGCGGTTTGTCAGCTCCGAACTGGAAGGCGCCTGCTCTGAGTACCTGGCACCGCTGA
  GCGCTGTGGACCTCAAGCTGTACCATGACCCCGACCTGCTGTTAGGTCCCGGTTGCGTGT
  ACCCTGCTGCCTCTGTGGCCCGCTTTGCCTCCCACTGGCGCCTTCCCCTGCTGACTGCGG
  GTGCTGTGGCCTCTGGTTTTTCGGCTAAGAATGACCATTATCGTACCCTGGTTCGCACTG
  GCCCCTCTGCTCCCAAGCTGGGTGAGTTTGTGGTGACACTACACGGGCACTTCAATTGGA
  CTGCCCGTGCTGCCTTGCTGTACCTGGATGCTCGCACAGATGACCGGCCTCACTACTTCA
  CCATCGAGGGCGTCTTTGAGGCCCTGCAGGGCAGCAACCTCAGTGTGCAGCACCAGGTGT
  ATGCCCGAGAGCCAGGGGGCCCCGAGCAGGCCACCCACTTCATCCGGGCCAACGGGCGCA
  TTGTGTATATCTGCGGCCCTCTGGAGATGCTGCATGAGATCCTGCTTCAGGCCCAGAGGG
  AGAATCTGACCAATGGGGATTATGTCTTCTTTTACCTGGATGTCTTTGGGGAGAGTCTCC
  GTGCAGGCCCCACACGTGCTACAGGCCGGCCCTGGCAGGACAATCGCACCCGGGAACAGG
  CCCAGGCCCTCAGAGAGGCCTTTCAGACTGTATTGGTGATCACGTACCGAGAACCCCCAA
  ATCCTGAGTATCAGGAATTCCAGAATCGTCTGCTGATAAGAGCCCGGGAAGACTTTGGTG
  TGGAGCTGGGCCCTTCCCTGATGAACCTCATCGCTGGCTGCTTCTATGATGGGATCCTGC
  TATATGCTGAAGTCCTGAATGAGACAATACAGGAAGGAGGCACCCGGGAGGATGGACTTC
  GAATTGTGGAAAAGATGCAGGGACGAAGATATCACGGTGTAACTGGGCTGGTTGTCATGG
  ACAAGAACAATGACCGAGAGACTGACTTTGTCCTCTGGGCCATGGGAGACCTGGATTCTG
  GGGACTTTCAGCCTGCAGCCCACTACTCGGGAGCTGAGAAGCAGATTTGGTGGACGGGAC
  GGCCTATTCCCTGGGTGAAGGGGGCTCCTCCCTCGGACAATCCCCCCTGTGCCTTTGACT
  TGGACGACCCATCCTGTGATAAAACTCCACTTTCAACCCTGGCAATTGTGGCTCTGGGCA
  CAGGAATCACCTTCATCATGTTTGGTGTTTCCAGCTTCCTAATTTTCCGAAAGCTGATGC
  TGGAGAAGGAGCTGGCTAGCATGTTGTGGCGTATTCGCTGGGAAGAACTGCAGTTTGGCA
  ACTCAGAGCGTTATCACAAAGGTGCAGGCAGTCGCCTCACACTGTCGCTGCGGGGATCCA
  GTTACGGCTCGCTCATGACAGCCCATGGGAAATACCAGATCTTTGCCAACACCGGTCACT
  TCAAGGGAAATGTTGTCGCCATCAAACATGTGAATAAGAAGCGCATTGAGCTGACCCGGC
  AGGTTCTGTTTGAACTCAAACATATGAGAGATGTTCAGTTCAACCATCTCACTCGCTTCA
  TTGGCGCCTGCATAGACCCTCCCAACATTTGCATTGTCACTGAATATTGTCCTCGTGGGA
  GTTTACAGGATATTCTAGAAAATGACAGCATCAACTTGGACTGGATGTTTCGTTATTCAC
  TCATTAATGACCTTGTTAAGGGCATGGCCTTTCTCCACAACAGCATTATTTCATCGCATG
  GGAGTCTCAAGTCCTCCAACTGTGTGGTGGATAGTCGTTTTGTGCTCAAAATCACAGACT
  ATGGCCTGGCCAGCTTCCGATCAACTGCTGAACCTGATGACAGCCATGCCCTCTATGCCA
  AGAAGCTGTGGACTGCCCCAGAACTGCTCAGTGGGAACCCCTTGCCAACCACAGGCATGC
  AGAAGGCTGACGTCTATAGCTTTGGGATCATCCTGCAGGAGATAGCACTTCGCAGTGGTC
  CTTTCTACTTGGAGGGCCTGGACCTCAGCCCCAAAGAGATTGTCCAGAAGGTACGAAATG
  GTCAGCGGCCATATTTCCGGCCAAGCATTGACCGGACCCAACTGAATGAAGAGCTAGTTT
  TGCTGATGGAGCGATGTTGGGCTCAGGACCCAGCTGAGCGGCCAGACTTTGGACAGATTA
  AGGGCTTCATTCGGCGCTTTAACAAGGAGGGTGGCACCAGCATATTGGACAACCTCCTGC
  TGCGCATGGAACAGTATGCCAATAACTTGGAGAAGCTGGTGGAGGAACGCACACAGGCCT
  ATCTGGAGGAAAAACGCAAGGCTGAAGCTCTGCTCTACCAAATCCTACCCCATTCAGTGG
  CAGAGCAGTTAAAACGGGGAGAGACTGTACAGGCTGAGGCCTTTGACAGTGTTACCATCT
  ACTTCAGTGACATTGTTGGCTTCACAGCATTGTCAGCAGAGAGCACCCCCATGCAGGTAG
  TGACACTTCTTAATGACCTGTATACCTGCTTTGATGCCATAATTGACAACTTTGATGTCT
  ACAAGGTGGAGACGATTGGGGATGCTTACATGGTGGTATCTGGCCTCCCAGGCCGAAATG
  GTCAACGCCATGCACCAGAAATTGCTCGTATGGCCCTAGCATTACTAGATGCAGTTTCTT
  CCTTTCGCATCCGCCACCGACCCCATGACCAGCTGAGGCTACGCATAGGGGTCCATACTG
  GGCCAGTCTGTGCTGGGGTTGTTGGCCTGAAGATGCCCCGTTATTGTCTTTTTGGAGACA
  CAGTGAACACTGCTTCTCGAATGGAGTCTAATGGTCAAGCGCTGAAGATCCATGTCTCCT
  CTACCACCAAGGATGCCCTAGATGAGCTAGGATGCTTCCAGCTAGAGCTTCGGGGGGATG
  TGGAAATGAAGGGAAAAGGAAAGATGCGAACATACTGGCTCTTAGGAGAGCGGAAAGGAC
  CTCCTGGACTCCTGTAAACCCCCATTCTTTCCAAGTCAGATAGTCTTCTGCTGCTGGTAC
  CTGGGTGGGCAATGGCCACCATGTCTGCACACACCAGAAATGGACATTTTCATATGCAAT
  GGAAAACAGCCACAAAAAAACCTACCTTATATGGAAGTTGTAGCCCTCTGCAGCTCAGCC
  CTGTACATATACCTGTCCCTCTCTGGCTTGGTCCCCTTCCTCCCTACTTTCTGTAAATAT
  CTGTATCTAAACCAGAATATTTTGGTCAAATATAAAACAATAATAAAAAAAGTTCTGATG
  TCAAAAAAAAAAAAAAAAACTCGA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003995 unedited
  NAAAATTGGCAGCCTGGCAGTGTGGTGCGTCCTCCCGGGCGCGGNAACCTGAGCTGGCGG
  TGGTGCTGCCAGAACACAACCTGAGCTATGCCTGGGCCTGGCCACGGGTGGGACCCGCTG
  TGGCACTAGCTGTGGAGGCTCTGGGCCGGGCACTGCCCGTGGACCTGCGGTTTGTCAGCT
  CCGAACTGGAAGGCGCCTGCTCTGAGTACCTGGCACCGCTGAGCGCTGTGGACCTCAAGC
  TGTACCATGACCCCGACCTGCTGTTAGGTCCCGGTTGCGTGTACCCTGCTGCCTCTGTGG
  CCCGCTTTGCCTCCCACTGGCGCCTTCCCCTGCTGACTGCGGGTGCTGTGGCCTCTGGTT
  TTTCGGCTAAGAATGACCATTATCGTACCCTGGTTCGCACTGGCCCCTCTGCTCCCAAGC
  TGGGTGAGTTTGTGGTGACACTACACGGGCACTTCAATTGGACTGCCCGTGCTGCCTTGC
  TGTACCTGGATGCTCGCACAGATGACCGGCCTCACTACTTCACCATCGAGGGCGTCTTTG
  AGGCCCTGCAGGGCAGCAACCTCAGTGTGCAGCACCAGGTGTATGCCCGAGAGCCAGGGG
  GCCCCGAGCAGGCCACCCACTTCATCCGGGCCAACGGGCGCATTGTGTATATCTGCGGCC
  CTCTGGAGATGCTGCATGAGATCCTGCTTCAGGCCCAGAGGGAGAATCTGACCAATGGGG
  ATTATGTCTTCTTTTACCTGGATGTCTTTGGGGAGAGTCTCCGTGCAGGCCCCACACGTG
  CTACAGGCCGGCCCTGGCAGGACAATCGCACCCGGGAACAGGCCCAGGCCCTCAGAGAGG
  CCTTTCAGACTGTATTGGTGATCACGTACCGAGAACCCCCAATCCTGAGTATCANGAATT
  CAGAATCGTC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003995 unedited
  NNNAATTACTGTGNACCGCGGCCCGCAATCTANGATCGAGTTTTTTTTTTTTTTTTTGAC
  ATCAGAACTTTTTTTATTATTGTTTTATATTTGACCAAAATATTCTGGTTTAGATACAGA
  TATTTACAGAAAGTAGGGAGGAAGGGGACCAAGCCAGAGAGGGACAGGTATATGTACAGG
  GCTGAGCTGCAGAGGGCTACAACTTCCATATAAGGTAGGTTTTTTTGTGGCTGTTTTCCA
  TTGCATATGAAAATGTCCATTTCTGGTGTGTGCAGACATGGTGGCCATTGCCCACCCAGG
  TACCAGCAGCAGAAGACTATCTGACTTGGAAAGAATGGGGGTTTACAGGAGTCCAGGAGG
  TCCTTTCCGCTCTCCTAAGAGCCAGTATGTTCGCATCTTTCCTTTTCCCTTCATTTCCAC
  ATCCCCCCGAAGCTCTAGCTGGAAGCATCCTAGCTCATCTAGGGCATCCTTGGTGGTAGA
  GGAGACATGGATCTTCAGCGCTTGACCATTAGACTCCATTCGAGAAGCAGTGTTCACTGT
  GTCTCCAAAAAGACAATAACGGGGCATCTTCAGGCCAACAACCCCAGCACAGACTGGCCC
  AGTATGGACCCCTATGCGTAGCCTCAGCTGGTCATGGGGTCGGTGGCGGATGCGAAAGGA
  AGAAACTGCATCTAGTAATGCTAGGGCCATACGAGCAATTTCTGGTGCATGGCGTTGACC
  ATTTCGGCCTGGGAGGCCAGATACCACCATGTAAGCATCCCCAATCGTCTCCACCTTGTA
  GACATCAAAGTTGTCAATTATGGCATCAAAGCAGGTATACAGGTCATTAAGAAGTGTCAC
  TACCTGCATGGGGGTGCTCTCTGCTGACAATGCTGTGAAGCCACATGTCACTGAATAGAT
  GGTACACTGTCAAAGCCTCAGC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003995
• Insert Size: 3300 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003995.3, NP_003986.2
• RefSeq Size: 3447 bp
• RefSeq ORF: 3144 bp
• Locus ID: 4882
• UniProt ID: P20594
• Cytogenetics: 9p13.3
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Purine metabolism, Vascular smooth muscle contraction
• Gene Summary: This gene encodes natriuretic peptide receptor B, one of two integral membrane receptors for natriuretic peptides. Both NPR1 and NPR2 contain five functional domains: an extracellular ligand-binding domain, a single membrane-spanning region, and intracellularly a protein kinase homology domain, a helical hinge region involved in oligomerization, and a carboxyl-terminal guanylyl cyclase catalytic domain. The protein is the primary receptor for C-type natriuretic peptide (CNP), which upon ligand binding exhibits greatly increased guanylyl cyclase activity. Mutations in this gene are the cause of acromesomelic dysplasia Maroteaux type. [provided by RefSeq, Jul 2008]