Angiotensinogen (AGT) (NM_000029) Human Untagged Clone

CAT#: SC117553

AGT (untagged)-Human angiotensinogen (serpin peptidase inhibitor, clade A, member 8) (AGT)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Angiotensinogen
• Synonyms: ANHU; hFLT1; SERPINA8
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC117553 sequence for NM_000029 edited (data generated by NextGen Sequencing)
  ATGCGGAAGCGAGCACCCCAGTCTGAGATGGCTCCTGCCGGTGTGAGCCTGAGGGCCACC
  ATCCTCTGCCTCCTGGCCTGGGCTGGCCTGGCTGCAGGTGACCGGGTGTACATACACCCC
  TTCCACCTCGTCATCCACAATGAGAGTACCTGTGAGCAGCTGGCAAAGGCCAATGCCGGG
  AAGCCCAAAGACCCCACCTTCATACCTGCTCCAATTCAGGCCAAGACATCCCCTGTGGAT
  GAAAAGGCCCTACAGGACCAGCTGGTGCTAGTCGCTGCAAAACTTGACACCGAAGACAAG
  TTGAGGGCCGCAATGGTCGGGATGCTGGCCAACTTCTTGGGCTTCCGTATATATGGCATG
  CACAGTGAGCTATGGGGCGTGGTCCATGGGGCCACCGTCCTCTCCCCAACGGCTGTCTTT
  GGCACCCTGGCCTCTCTCTATCTGGGAGCCTTGGACCACACAGCTGACAGGCTACAGGCA
  ATCCTGGGTGTTCCTTGGAAGGACAAGAACTGCACCTCCCGGCTGGATGCGCACAAGGTC
  CTGTCTGCCCTGCAGGCTGTACAGGGCCTGCTAGTGGCCCAGGGCAGGGCTGATAGCCAG
  GCCCAGCTGCTGCTGTCCACGGTGGTGGGCGTGTTCACAGCCCCAGGCCTGCACCTGAAG
  CAGCCGTTTGTGCAGGGCCTGGCTCTCTATACCCCTGTGGTCCTCCCACGCTCTCTGGAC
  TTCACAGAACTGGATGTTGCTGCTGAGAAGATTGACAGGTTCATGCAGGCTGTGACAGGA
  TGGAAGACTGGCTGCTCCCTGATGGGAGCCAGTGTGGACAGCACCCTGGCTTTCAACACC
  TACGTCCACTTCCAAGGGAAGATGAAGGGCTTCTCCCTGCTGGCCGAGCCCCAGGAGTTC
  TGGGTGGACAACAGCACCTCAGTGTCTGTTCCCATGCTCTCTGGCATGGGCACCTTCCAG
  CACTGGAGTGACATCCAGGACAACTTCTCGGTGACTCAAGTGCCCTTCACTGAGAGCGCC
  TGCCTGCTGCTGATCCAGCCTCACTATGCCTCTGACCTGGACAAGGTGGAGGGTCTCACT
  TTCCAGCAAAACTCCCTCAACTGGATGAAGAAACTGTCTCCCCGGACCATCCACCTGACC
  ATGCCCCAACTGGTGCTGCAAGGATCTTATGACCTGCAGGACCTGCTCGCCCAGGCTGAG
  CTGCCCGCCATTCTGCACACCGAGCTGAACCTGCAAAAATTGAGCAATGACCGCATCAGG
  GTGGGGGAGGTGCTGAACAGCATTTTTTTTGAGCTTGAAGCGGATGAGAGAGAGCCCACA
  GAGTCTACCCAACAGCTTAACAAGCCTGAGGTCTTGGAGGTGACCCTGAACCGCCCATTC
  CTGTTTGCTGTGTATGATCAAAGCGCCACTGCCCTGCACTTCCTGGGCCGCGTGGCCAAC
  CCGCTGAGCACAGCATGA
  
  Clone variation with respect to NM_000029.3
  1116 a=>g
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000029 unedited
  GTGTGCACTATTTTGTNAATACGACNCACTTATAGGGCGGCCGCGATTCGGCACGAGGTT
  GTTCTGGGTACTACAGCAGAAGGGTATGCGGAAGCGAGCACCCCAGTCTGAGATGGCTCC
  TGCCGGTGTGAGCCTGAGGGCCACCATCCTCTGCCTCCTGGCCTGGGCTGGCCTGGCTGC
  AGGTGACCGGGTGTACATACACCCCTTCCACCTCGTCATCCACAATGAGAGTACCTGTGA
  GCAGCTGGCAAAGGCCAATGCCGGGAAGCCCAAAGACCCCACCTTCATACCTGCTCCAAT
  TCAGGCCAAGACATCCCCTGTGGATGAAAAGGCCCTACAGGACCAGCTGGTGCTAGTCGC
  TGCAAAACTTGACACCGAAGACAAGTTGAGGGCCGCAATGGTCGGGATGCTGGCCAACTT
  CTTGGGCTTCCGTATATATGGCATGCACAGTGAGCTATGGGGCGTGGTCCATGGGGCCAC
  CGTTCTCTCCCCAACGGCTGTCTTTGGCACCCTGGCCTCTCTCTATCTGGGAGCCTTGGA
  CCACACAGCTGACAGGCTACAGGCAATCCTGGGTGTTCCTTGGAAGGACAAGAACTGCAC
  CTCCCCGGCTGGATGCGCACAAGGCCCCTGTCTGCCCTGCAGGCTGTACAGGGCCCTGCT
  AATGGCCCAAGGCAGGGCTGATAGCCAGGCCCAGCTGCTGCTGTCCACGTGGTGGGCGTG
  TTCACACTCCCAGCCTGCACTCGAACCACCGTTTGTGCAGGCCCTGCTTTCTATAACCCC
  TGAGGTCCTCCACCCTCTCTGGACTTCACAAAATGGATGTGCCGCTGACAAAAGGCAGGT
  TCATGCCCCCTGTGCACCGAAGGAAGACCGGCTGTTCCCTGATGGGACCACCGTGGAACA
  CACACCTGCCTT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000029 unedited
  GGCAACTTTCCAGGGCCAGGNATAGCACTGGGGAGGGGTCACAGGGCAGCCACCCGGGAT
  CTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTT
  TTTGTGGCAAGACGTTTATTACTAACACAAGGGAGAAATAACCAGCTATTGTTCCGCATT
  CAAACAGAAATTCAGGTGCTTGCATCTTTCACGTATTGGTCAAAAATCACAAGCATCTGT
  GGAAAAAACTAAGGTATTACAGACACTACACGGAGGTCATGTTCTTACATTCAAGACACT
  AAATACAAACCGAAGGCAATGCAAAAATGTATACTTTAATTTTAAAACCCAATTTTTGTT
  CTCAACTTGAAAAGGGAACACTTTTTTGTTTCACAAACAAGCTGGTCGGTTGGAATTCTT
  TTTGGAACAGTAGTCCCGCGCTAAACACTGGTTCTTGCCTCCCCACCCCCATTCTCTAAA
  ATAAACCCAGCAAACTGGGAGGTGCATTTGTGCCGCTGCAGGCTTCTACTGCTCACTCCA
  TGCAGCACACTTAGACCAAGGAGAAACGGCTGCTTTCCAGCTCAAAGTCGACTATTAAAA
  GAAAAGGTGGGAGACTGGGGGTGACCATCCTGATTGTCCCCGGGGGGTGGTATCTGGCTG
  CTGGCCTTTGCCTCAAAGCCAAGGGCAAAAGCCTTGCAGCAACTGGGTTTTTGGGGGGCC
  TGGCTCATGCTGGCTAAAGGGGGTTGGCCCCCCGCCCACAAAAGCAGGGGCAATGGGGCT
  TTTTAATCTCCACACAGAAAGGGGGGGGGTTGGAGGCCCCCCCCAACCTAGGCTGTAAAA
  GCCGGGGGGGAAACCTTGGGGGGCCTCTTATAACGGTAAGCCAAAAAAGGCGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000029
• Insert Size: 2050 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000029.2
• RefSeq Size: 2192 bp
• RefSeq ORF: 1458 bp
• Locus ID: 183
• UniProt ID: P01019
• Cytogenetics: 1q42.2
• Domains: SERPIN
• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: Renin-angiotensin system
• Gene Summary: The protein encoded by this gene, pre-angiotensinogen or angiotensinogen precursor, is expressed in the liver and is cleaved by the enzyme renin in response to lowered blood pressure. The resulting product, angiotensin I, is then cleaved by angiotensin converting enzyme (ACE) to generate the physiologically active enzyme angiotensin II. The protein is involved in maintaining blood pressure, body fluid and electrolyte homeostasis, and in the pathogenesis of essential hypertension and preeclampsia. Mutations in this gene are associated with susceptibility to essential hypertension, and can cause renal tubular dysgenesis, a severe disorder of renal tubular development. Defects in this gene have also been associated with non-familial structural atrial fibrillation, and inflammatory bowel disease. [provided by RefSeq, Nov 2019]