Aurora B (AURKB) (NM_004217) Human Untagged Clone

CAT#: SC117526

AURKB (untagged)-Human aurora kinase B (AURKB)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Aurora B
• Synonyms: AIK2; AIM-1; AIM1; ARK-2; ARK2; AurB; aurkb-sv1; aurkb-sv2; IPL1; PPP1R48; STK-1; STK5; STK12
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC117526 sequence for NM_004217 edited (data generated by NextGen Sequencing)
  ATGGCCCAGAAGGAGAACTCCTACCCCTGGCCCTACGGCCGACAGACGGCTCCATCTGGC
  CTGAGCACCCTGCCCCAGCGAGTCCTCCGGAAAGAGCCTGTCACCCCATCTGCACTTGTC
  CTCATGAGCCGCTCCAATGTCCAGCCCACAGCTGCCCCTGGCCAGAAGGTGATGGAGAAT
  AGCAGTGGGACACCCGACATCTTAACGCGGCACTTCACAATTGATGACTTTGAGATTGGG
  CGTCCTCTGGGCAAAGGCAAGTTTGGAAACGTGTACTTGGCTCGGGAGAAGAAAAGCCAT
  TTCATCGTGGCGCTCAAGGTCCTCTTCAAGTCCCAGATAGAGAAGGAGGGCGTGGAGCAT
  CAGCTGCGCAGAGAGATCGAAATCCAGGCCCACCTGCACCATCCCAACATCCTGCGTCTC
  TACAACTATTTTTATGACCGGAGGAGGATCTACTTGATTCTAGAGTATGCCCCCCGCGGG
  GAGCTCTACAAGGAGCTGCAGAAGAGCTGCACATTTGACGAGCAGCGAACAGCCACGATC
  ATGGAGGAGTTGGCAGATGCTCTAATGTACTGCCATGGGAAGAAGGTGATTCACAGAGAC
  ATAAAGCCAGAAAATCTGCTCTTAGGGCTCAAGGGAGAGCTGAAGATTGCTGACTTCGGC
  TGGTCTGTGCATGCGCCCTCCCTGAGGAGGAAGACAATGTGTGGCACCCTGGACTACCTG
  CCCCCAGAGATGATTGAGGGGCGCATGCACAATGAGAAGGTGGATCTGTGGTGCATTGGA
  GTGCTTTGCTATGAGCTGCTGGTGGGGAACCCACCCTTTGAGAGTGCATCACACAACGAG
  ACCTATCGCCGCATCGTCAAGGTGGACCTAAAGTTCCCCGCTTCTGTGCCCACGGGAGCC
  CAGGACCTCATCTCCAAACTGCTCAGGCATAACCCCTCGGAACGGCTGCCCCTGGCCCAG
  GTCTCAGCCCACCCTTGGGTCCGGGCCAACTCTCGGAGGGTGCTGCCTCCCTCTGCCCTT
  CAATCTGTCGCCTGA
  
  Clone variation with respect to NM_004217.2
  885 c=>t;893 t=>c
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004217 unedited
  NAGGTCGAGATTTTGTAAACGACTTACTATAGGCGGCCGCGAATTCGCACGGGGGGGCGG
  CCGGGAGAGTAGCAGTGCCTTGGACCCCAGCTCTCCTCCCCCTTTCTCTCTAAGGATGGC
  CCAGAAGGAGAACTCCTACCCCTGGCCCTACGGCCGACAGACGGCTCCATCTGGCCTGAG
  CACCCTGCCCCAGCGAGTCCTCCGGAAAGAGCCTGTCACCCCATCTGCACTTGTCCTCAT
  GAGCCGCTCCAATGTCCAGCCCACAGCTGCCCCTGGCCAGAAGGTGATGGAGAATAGCAG
  TGGGACACCCGACATCTTAACGCGGCACTTCACAATTGATGACTTTGAGATTGGGCGTCC
  TCTGGGCAAAGGCAAGTTTGGAAACGTGTACTTGGCTCGGGAGAAGAAAAGCCATTTCAT
  CGTGGCGCTCAAGGTCCTCTTCAAGTCCCAGATAGAGAAGGAGGGCGTGGAGCATCAGCT
  GCGCAGAGAGATCGAAATCCAGGCCCACCTGCACCATCCCAACATCCTGCGTCTCTACAA
  CTATTTTTATGACCGGAGGAGGATCTACTTGATTCTAGAGTATGCCCCCCGCGGGGAGCT
  CTACAAGGAGCTGCAGAAGAGCTGCACATTTGACGAGCAGCGAACAGCCACGATCATGGA
  GGAGTTGGCAGATGCTCTAATGTACTGCCATGGGAAGAAGGTGATTCACAGAGACATAAA
  GCCAGATAATCTGCTCTTAGGGCTCAAGGGAGAGCTGAAGATTGCTGACTTCGGCTGGTC
  TGTGCATGCGCCCTCCCTGACGAGGAAAGACATGTGTGGCACCCTGGACTACCTGCCCTC
  AGAATGATTGAGGGGCGCATGCACATGAGTAGGTGGATCTGTGGTGCATTGGAGTGCCTT
  GCTATGAGCTGCTGGTGGGGCACCCACN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004217 unedited
  GTACACAGTGGCATGCCCACTCCGGGTATCTGTTGCAGGAAAACAGCTATGACCGCGGCC
  GCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTACAAAAAGCTTCAGCCTTTATTAAAC
  AAAGGAGGAGGTAGAAAACAGATAAGGGAACAGTTAGGGATCCCTTCTTTCCCCTATACA
  TACACAGACATACAAACACACGCACCCGAGTGAATGACAGGGACCATCAGGCGACAGATT
  GAAGGGCAGAGGGAGGCAGCACCCTCCGAGAGTTGGCCCGGACCCAAGGGTGGGCTGAGA
  CCTGGGCCAGGGGCAGCCGTTCCGAGGGGTTATGCCTGACCAGTTTGGAGATGAAGTCCT
  GGGCTCCCGTGGGCACAGAAGCGGGGAACTTTAGGTCCACCTTGACAATACGGCGATAGG
  TCTCGTTGTGTGATGCACTCTCAAAGGGGTGGGTTCCCCACCAGCAGCTCATAGCATAGC
  ACTCCAATGCACCACATATCCACCTTCTCATTGTGCATGCGCCCCTCAATCATCTCTGGG
  GGCAGGTAGTCCAGGGTGCCACACATTGTCTTCCTCCTCAGGGAGGGCGCATGCACAGAC
  CAGCCCGAAGTCAGCATCTTCAGCTCTCCCTTGAGCCCTAANACAAATTTTCTGGCTTTA
  TGTCTCTGAGAACACCTTCTTCCCATGGCAGTACATTAGAGCATCTGCCAACTCCTCCAT
  GATCGTGGGCTGTTCGCTGCTCGTCAAATGTGCAGCTCTTCTGCAGCTCCTTGTAAAGCT
  CCCCGCGGGGGGCATACTCTAAATCAAGTTGATCCTCCTCCCGTCATAAAATATTGTAAA
  ACCCCAGATGTTGGGATGGTTCCAAGTGGCCCTGGATTTTTATCTCTCGCACAACTGATG
  CCCACCCT
  
• Restriction Sites: Please inquire
• ACCN: NM_004217
• Insert Size: 1300 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004217.1, NP_004208.1
• RefSeq Size: 1224 bp
• RefSeq ORF: 1035 bp
• Locus ID: 9212
• UniProt ID: Q96GD4
• Cytogenetics: 17p13.1
• Protein Families: Druggable Genome, Protein Kinase, Stem cell - Pluripotency
• Gene Summary: This gene encodes a member of the aurora kinase subfamily of serine/threonine kinases. The genes encoding the other two members of this subfamily are located on chromosomes 19 and 20. These kinases participate in the regulation of alignment and segregation of chromosomes during mitosis and meiosis through association with microtubules. A pseudogene of this gene is located on chromosome 8. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Sep 2015]
  Transcript Variant: This variant (1) represents the predominant transcript and encodes the predominant isoform (1). Variants 1 and 4 encode the same isoform.