ASCL1 (NM_004316) Human Untagged Clone

CAT#: SC117427

ASCL1 (untagged)-Human achaete-scute complex homolog 1 (Drosophila) (ASCL1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ASCL1
• Synonyms: ASH1; bHLHa46; HASH1; MASH1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_004316, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGAAAGCTCTGCCAAGATGGAGAGCGGCGGCGCCGGCCAGCAGCCCCAGCCGCAGCCCCAGCAGCCCT
  TCCTGCCGCCCGCAGCCTGTTTCTTTGCCACGGCCGCAGCCGCGGCGGCCGCAGCCGCCGCAGCGGCAGC
  GCAGAGCGCGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGGCGCCGCAGCTGAGACCGGCGGCC
  GACGGCCAGCCCTCAGGGGGCGGTCACAAGTCAGCGCCCAAGCAAGTCAAGCGACAGCGCTCGTCTTCGC
  CCGAACTGATGCGCTGCAAACGCCGGCTCAACTTCAGCGGCTTTGGCTACAGCCTGCCGCAGCAGCAGCC
  GGCCGCCGTGGCGCGCCGCAACGAGCGCGAGCGCAACCGCGTCAAGTTGGTCAACCTGGGCTTTGCCACC
  CTTCGGGAGCACGTCCCCAACGGCGCGGCCAACAAGAAGATGAGTAAGGTGGAGACACTGCGCTCGGCGG
  TCGAGTACATCCGCGCGCTGCAGCAGCTGCTGGACGAGCATGACGCGGTGAGCGCCGCCTTCCAGGCAGG
  CGTCCTGTCGCCCACCATCTCCCCCAACTACTCCAACGACTTGAACTCCATGGCCGGCTCGCCGGTCTCA
  TCCTACTCGTCGGACGAGGGCTCTTACGACCCGCTCAGCCCCGAGGAGCAGGAGCTTCTCGACTTCACCA
  ACTGGTTCTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004316 unedited
  TGTTANATTTTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACGAGGCTGGCCAGG
  GAACGTGGAAGGCGCACCGACAGGNATCCGGCCAGGGAGGGCGAGTGAAAGAAGGAAATC
  AGAAAGGAAGGGAGTTAACAAAATAATAAAAACAGCCTGAGCCACGGCTGGAGAGACCGA
  GACCCGGCGCAAGAGAGCGCAGCCTTAGTAGGAGAGGAACGCGAGACGCGGCAGAGCGCG
  TTCAGCACTGACTTTTGCTGCTGCTTCTGCTTTTTTTTTTCTTAGAAACAAGAAGGCGCC
  AGCGGCAGCCTCACACGCGAGCGCCACGCGAGGCTCCCGAAGCCAACCCGCGAAGGGAGG
  AGGGGAGGGAGGAGGAGGCGGCGTGCAGGGAGGAGAAAAAGCATTTTCACTTTTTTTGCT
  CCCACTCTAAGAAGTCTCCCGGGGATTTTGTATATATTTTTTAACTTCCGTCAGGGCTCC
  CGCTTCATATTTCCTTTTCTTTCCCTCTCTGTTCCTGCACCCAAGTTCTCTCTGTGTCCC
  CCTCGCGGGCCCCGCACCTCGCGTCCCGGATCGCTCTGATTCCGCGACTCCTTGGCCGCC
  GCTGCGCATGGAAAGCTCTGCCAAGATGGAGAGCGGCGGCGCCGGCCAGCAGCCCCAGCC
  GCAGCCCCAGCAGCCCTTCCTGCCGCCCGCAGCCTGTTTCTTTGCCACGGCCGCAGCCGC
  CGGCGGCCGCAGCCGCCGCAGCGGCAGCGCAGAGCGCGCAGCAGCAGCAGCAGCAGCAGC
  AGCAGCAGCAGCAGGCGCCGCAGCTGAGACCGGCGGCCGACGCCAGCCTCAGGGGGCCGG
  TCACAAGTCAGCGCCCAAGCAGTCAAGCGAAGCGCTCGTCTTCGNCCGACTGATGCGCTG
  CAACGCCGCTCACTCAGCGGCTTT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004316 unedited
  ACTTAAAGAGGGAAAAGACTTATTACTGAACTAGTTTGTATAAAACAGGGTTATACATAT
  TTTTGTAAGTTTGTAATAAAACAGTAAGAAAAAAAAGCAGTAATAGAAATCTCCAAAAGG
  CAACCTATCAAAACCAACTGGCTGCCACTTTGAGTTTGGACAGTAGCTGCATAAACTTTG
  TTCTTCTTGAACAGTATTTAATAACATCATTAATACATTAACAACATTTCTATAAAGTAA
  GACACATTGGTGCTGAAGTACAACTGGTGGCCTCTTGATCTCACCTATGAGGAGAGTTCT
  TTACAAAACCACATAGGGAAAATTGCAGTTGTAAGGTGAACTACACATCTAAAATATGCA
  GAGGTAATAGCATTACATGTTAAAGTATCAAGATATACACATTTTAAACCATTTGTACAA
  AACTTCTATAAATTTTTCTCTCTCTTTCTCTCTTATGTACAAAAATATCTTAATATATCC
  CCGAACTGGTTAGGATAGATACAAATAGATTTTTTATAATAAAAAATTCACAAAAGATTG
  GAAGCATTCTATAATGAAAATGGTAGAAAAGACAGTGTGAGGGAAGCCATGGGGTTTGGG
  AATCGGGCCCTGGAGGAGAAGCAGAGTTTCAAAGGGCTGAGAATAGCATAGTTTCACTGT
  AAACCAATGTCTACAGCTTATTGGGGTGGGGGCTACTGAGACGAAAGACACCAACTTCGT
  TTCTAGAAGGCTAAGAACTGCCCTCTAAGAAGGGCGGCGAGGTGAAGGGACCCGAGCATG
  ACCTTCAGCTGTGCCGTGTATAGTGATGGGAGTACTATAACGCGTGTGCTGCTCCCTTCT
  GCCAGAACCCACTGCTTTTGCCACCAGCTGCATTTTGCGCATTGACTCAGTTCCAGTTGC
  TCTCTTATCTCCCGGATGATTGAGAGCAAAAAC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004316
• Insert Size: 2600 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004316.2, NP_004307.2
• RefSeq Size: 2482 bp
• RefSeq ORF: 711 bp
• Locus ID: 429
• UniProt ID: P50553
• Cytogenetics: 12q23.2
• Domains: HLH
• Protein Families: Druggable Genome, Transcription Factors
• Gene Summary: This gene encodes a member of the basic helix-loop-helix (BHLH) family of transcription factors. The protein activates transcription by binding to the E box (5'-CANNTG-3'). Dimerization with other BHLH proteins is required for efficient DNA binding. This protein plays a role in the neuronal commitment and differentiation and in the generation of olfactory and autonomic neurons. Mutations in this gene may contribute to the congenital central hypoventilation syndrome (CCHS) phenotype in rare cases. [provided by RefSeq, Jul 2008]