GSDME (NM_004403) Human Untagged Clone

CAT#: SC117401

DFNA5 (untagged)-Human deafness, autosomal dominant 5 (DFNA5), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GSDME
• Synonyms: DFNA5; ICERE-1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC117401 sequence for NM_004403 edited (data generated by NextGen Sequencing)
  ATGTTTGCCAAAGCAACCAGGAATTTTCTTAGAGAAGTTGATGCTGATGGTGACCTGATT
  GCAGTATCAAATCTGAATGACTCTGATAAGTTACAGCTTCTAAGTCTGGTGACAAAAAAG
  AAGAGATTCTGGTGCTGGCAGAGACCCAAGTACCAGTTTTTATCCCTCACCCTTGGCGAT
  GTACTCATAGAAGACCAATTTCCGAGTCCAGTGGTCGTGGAGTCGGACTTTGTGAAATAC
  GAGGGCAAGTTTGCAAACCACGTGAGTGGAACCCTGGAGACTGCACTGGGGAAGGTCAAG
  CTGAACCTGGGGGGCAGCAGCCGCGTAGAGAGCCAGTCTTCATTTGGAACCCTGAGGAAG
  CAGGAGGTGGATTTGCAGCAGCTCATCAGAGACTCTGCCGAGAGAACAATAAATCTGAGA
  AACCCTGTGCTCCAGCAGGTGCTGGAAGGAAGGAATGAGGTCCTGTGCGTTTTGACACAG
  AAGATCACGACGATGCAGAAGTGTGTGATCTCTGAGCACATGCAGGTCGAGGAGAAGTGT
  GGTGGCATCGTGGGCATCCAGACCAAGACGGTGCAGGTGTCAGCGACGGAGGATGGGAAT
  GTCACCAAGGACTCCAACGTGGTGCTGGAGATCCCAGCTGCCACCACCATTGCCTACGGT
  GTCATTGAGTTATACGTGAAACTGGACGGCCAGTTCGAGTTCTGCCTTCTCCGAGGGAAG
  CAAGGTGGCTTCGAGAACAAGAAGAGAATTGACTCTGTCTACCTGGACCCCCTGGTCTTT
  CGAGAGTTTGCATTCATAGACATGCCAGATGCTGCGCATGGGATATCTTCCCAGGATGGA
  CCATTAAGTGTTTTAAAGCAAGCGACCCTGCTCCTGGAGAGGAATTTCCATCCATTTGCG
  GAGCTGCCTGAGCCACAACAGACAGCTTTGAGTGACATCTTCCAGGCGGTCCTATTTGAT
  GATGAACTACTCATGGTCCTGGAACCAGTGTGCGATGACCTGGTCAGCGGCCTCTCGCCC
  ACAGTGGCGGTGCTGGGGGAGCTGAAGCCCCGGCAGCAGCAGGACCTTGTGGCCTTCCTG
  CAGCTGGTGGGGTGCAGCTTACAGGGTGGGTGTCCGGGCCCCGAGGATGCAGGCAGCAAG
  CAGCTGTTTATGACAGCCTACTTCTTGGTCAGTGCCCTCGCAGAAATGCCAGATAGCGCA
  GCAGCTCTGCTGGGCACTTGCTGCAAACTCCAGATCATTCCCACACTGTGCCACTTGCTT
  CGTGCTCTGTCTGATGATGGAGTATCTGATCTTGAAGACCCAACCTTGACTCCCCTGAAA
  GATACAGAAAGGTTTGGGATTGTGCAGCGCTTGTTTGCCTCAGCTGACATTAGTCTGGAG
  AGACTGAAGTCATCTGTGAAAGCTGTCATTCTGAAGGACTCTAAAGTCTTCCCACTGCTT
  CTTTGTATAACCCTGAATGGACTCTGTGCTTTAGGCAGAGAACATTCATGA
  
  Clone variation with respect to NM_004403.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004403 unedited
  GGGCAATTTTTGTATACGACTCCTATAGGCGGCNCGCGATTCGGCACGAGGCTGGAGCTT
  TCAAAAGTTTGCCAAAGCAACCAGGAATTTTCTTAGAGAAGTTGATGCTGATGGTGACCT
  GATTGCAGTATCAAATCTGAATGACTCTGATAAGTTACAGCTTCTAAGTCTGGTGACAAA
  AAAGAAGAGATTCTGGTGCTGGCAGAGACCCAAGTACCAGTTTTTATCCCTCACCCTTGG
  CGATGTACTCATAGAAGACCAATTTCCGAGTCCAGTGGTCGTGGAGTCGGACTTTGTGAA
  ATACGAGGGCAAGTTTGCAAACCACGTGAGTGGAACCCTGGAGACTGCACTGGGGAAGGT
  CAAGCTGAACCTGGGGGGCAGCAGCCGCGTAGAGAGCCAGTCTTCATTTGGAACCCTGAG
  GAAGCAGGAGGTGGATTTGCAGCAGCTCATCAGAGACTCTGCCGAGAGAACAATAAATCT
  GAGAAACCCTGTGCTCCAGCAGGTGCTGGAAGGAAGGAATGAGGTCCTGTGCGTTTTGAC
  ACAGAAGATCACGACGATGCAGAAGTGTGTGATCTCTGAGCACATGCAGGTCGAGGAGAA
  GTGTGGTGGCATCGTGGGCATCCAGACCAAGACGGTGCAGGTGTCAGCGACGGAGGATGG
  GAATGTCACCAAGGACTCCAACGTGGTGCTGGAGATCCCAGCTGCCACCACCATTGCCTA
  CGGTGTCATTGAGTTATACGTGAAACTGGACGGCCAGTTCGAGTTCTGCCTTCTCTCGAG
  GAAGCAAGGTGGCTTCCAGAACCAAGAGAGAATTGACTCTGTCTACCTGGACCCCTGGTC
  TTTCCAGAGTTTGCATTCATANACTGCCAGATGCTGCGCATGGGAATCTTCCCAGAAGAG
  ACATTAAATGTTT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004403 unedited
  CCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTAGTTCAAAGTTTCCTTTA
  ATAGAATACCCAGAATACATAGNGAAATCAACATGGTGCTAGACATAGAATTAGTATGTT
  CACTGAAGCATGGCAAGGTCACATAAAATGCATATAGCTAGGGTCCCAAGCAGGGGTGTG
  TTACTGTTAGATTTTTTTGAAGTTTAATTTTGTTCAGCAGAGGAATATACTCTAGGAGCA
  TTTACAGTTCATTTTCAGTCATCAAATAACATACATCACTTCCAAAACGTAGCCTCTTGT
  GTGCAGAGAAATTGCCTTCCCACAGCATTCACAATGTAAAAAGACCTTTTGGATTAAAGG
  GTCAGACTCTTTCTATGTAACAAAAAGTGGAATGCAAGTGACAGATGGACTTATTATTGT
  GCAGAAAAAACGTCTGAATGTATGCTAAGAATTCTCCGCCCTCCCAGCTCTTTACATGCT
  GGAACCTAACTCAGATGCTGGGTCACCAGCACAGGAGGGCCTCTGATAAGGAAAACTGTT
  TAAAGAGTACCTGGTGGCTAAAAGTCAGGTCATTCATCATGCAAAATGTCACCACTTCTT
  AAACTGTTCTGTAAATTCATTTCATTGGTCAACTTTTAACGTGCATATGACCTTTTACAG
  TTCTGAAAAATAGCCTTGGCCAGTAACACGTACTTCTAGTTCACATATGACATCATGAAT
  GTTCTCTGCCTAAGCACAGAGTTCATTCAGGGTTATACAAAGAAGCAGGGGGGAGAACTT
  AGAGGTCTTCGAATGACAGCTTTCACGATGACTTTAGTCTCTTCAGACTAATGTCAACTG
  AGGCAAACAAGCGCTGCACCATCCCAAACCTTTCTGGATTTTTCCGGGGAGTCAGGGTTT
  GGGTCTTCAAGATCAGATACTCCTTTCTCAGACGGACCCGAAACAGTGGGCCAATNTGGG
  AATGATC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004403
• Insert Size: 2260 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004403.1, NP_004394.1
• RefSeq Size: 2230 bp
• RefSeq ORF: 1491 bp
• Locus ID: 1687
• UniProt ID: O60443
• Cytogenetics: 7p15.3
• Domains: DFNA5
• Protein Families: Druggable Genome
• Gene Summary: Hearing impairment is a heterogeneous condition with over 40 loci described. The protein encoded by this gene is expressed in fetal cochlea, however, its function is not known. Nonsyndromic hearing impairment is associated with a mutation in this gene. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (a). Variants 1 and 2 both encode isoform a.