DREF (ZBED1) (NM_004729) Human Untagged Clone

CAT#: SC117180

ZBED1 (untagged)-Human zinc finger, BED-type containing 1 (ZBED1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DREF
• Synonyms: ALTE; DREF; hDREF; TRAMP
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC117180 sequence for NM_004729 edited (data generated by NextGen Sequencing)
  ATGGAGAATAAAAGCCTGGAGAGCTCCCAGACAGACCTGAAGCTGGTGGCCCACCCCCGC
  GCCAAGAGCAAGGTGTGGAAGTATTTCGGCTTCGACACCAACGCCGAGGGATGCATCCTG
  CAGTGGAAGAAAATCTACTGCCGCATCTGCATGGCCCAGATCGCCTACTCCGGAAACACC
  TCCAACCTGTCCTACCACCTGGAGAAGAACCACCCCGAGGAATTCTGCGAGTTCGTCAAG
  AGCAACACGGAGCAGATGCGTGAAGCCTTCGCCACCGCCTTCTCCAAGCTGAAGCCCGAG
  TCGTCCCAGCAGCCCGGGCAGGACGCGCTGGCCGTCAAGGCCGGCCACGGCTACGACAGC
  AAGAAGCAGCAGGAGCTGACGGCCGCCGTGCTGGGCCTCATCTGCGAGGGGCTGTACCCA
  GCCTCCATCGTGGACGAGCCCACCTTCAAGGTGCTGCTGAAGACGGCCGACCCCCGGTAT
  GAGCTGCCCAGCCGGAAGTACATCTCTACCAAGGCCATCCCTGAGAAGTACGGGGCCGTC
  CGGGAGGTGATCCTGAAGGAGCTGGCCGAGGCCACCTGGTGTGGCATCTCCACCGACATG
  TGGAGGAGTGAGAATCAGAACCGCGCCTACGTCACGCTGGCCGCCCACTTCCTGGGCCTG
  GGCGCCCCCAACTGCCTGTCCATGGGCTCCCGCTGCCTGAAGACCTTCGAGGTGCCCGAA
  GAGAACACGGCGGAGACCATCACGCGAGTGCTCTATGAGGTCTTCATCGAGTGGGGCATC
  AGCGCCAAGGTCTTCGGGGCCACCACCAACTATGGCAAGGACATCGTGAAGGCGTGCTCC
  CTGCTGGACGTCGCAGTGCACATGCCCTGCCTGGGCCACACCTTCAATGCCGGCATCCAG
  CAGGCCTTCCAGCTCCCGAAGCTGGGGGCGCTGCTGTCGCGCTGCCGCAAACTGGTGGAG
  TACTTCCAGCAGTCTGCCGTGGCCATGTACATGCTCTATGAGAAGCAGAAGCAGCAGAAC
  GTGGCCCACTGCATGCTGGTGAGCAACCGCGTCTCCTGGTGGGGGAGCACGCTGGCCATG
  CTGCAGCGCCTCAAGGAGCAGCAGTTCGTCATCGCCGGGGTCTTGGTGGAGGACAGCAAC
  AACCACCACCTCATGCTGGAGGCCAGCGAGTGGGCCACCATCGAGGGGCTGGTGGAGCTC
  CTGCAGCCCTTCAAGCAGGTGGCCGAGATGCTGTCGGCCTCCAGGTACCCCACCATCAGC
  ATGGTGAAGCCGCTGCTGCACATGCTCCTGAACACCACGCTCAACATCAAGGAGACCGAC
  TCCAAGGAGCTCAGCATGGCCAAGGAGGTCATCGCCAAGGAGCTTTCCAAGACCTACCAG
  GAGACGCCCGAGATCGACATGTTTCTCAACGTGGCCACCTTCCTGGACCCCCGCTACAAG
  AGGCTGCCCTTCCTCTCCGCCTTCGAGCGGCAGCAGGTGGAGAATCGCGTGGTGGAAGAG
  GCCAAGGGCCTGCTGGACAAGGTCAAAGACGGCGGCTACCGGCCGGCTGAGGACAAGATC
  TTCCCGGTGCCCGAGGAGCCTCCCGTCAAGAAGCTCATGCGGACATCCACGCCGCCGCCC
  GCCAGCGTCATCAACAACATGCTGGCCGAGATCTTCTGCCAGACAGGCGGCGTGGAGGAC
  CAGGAAGAGTGGCATGCCCAGGTGGTGGAGGAGCTGAGCAACTTCAAGTCCCAGAAGGTG
  CTTGGCCTCAACGAAGACCCCCTCAAGTGGTGGTCAGACCGCCTGGCCCTCTTCCCCCTG
  CTGCCCAAGGTGCTGCAGAAGTACTGGTGCGTGACGGCCACGCGCGTCGCCCCTGAGCGT
  CTCTTCGGATCCGCCGCCAACGTGGTCAGCGCCAAGAGGAACCGGCTGGCTCCCGCGCAC
  GTGGACGAGCAGGTGTTTCTGTATGAGAACGCCCGGAGTGGGGCAGAGGCGGAACCCGAG
  GACCAGGACGAGGGGGAGTGGGGCCTGGACCAGGAGCAGGTGTTCTCCTTGGGGGATGGC
  GTCAGCGGCGGTTTCTTTGGCATTAGGGACAGCAGCTTCCTGTAG
  
  Clone variation with respect to NM_004729.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004729 unedited
  GGATTTTGTATCACGACTCACTATAGNNGGCGGCCGCGNAATTCGGCACGAGGGCGGGGG
  CTTCCTGGAGCCAGCAGGTCCCCACGCATGAGCAGCCGTCCAGCAGGCAGGCTCCGGTGG
  AGAAGCAATGGAGAATAAAAGCCTGGAGAGCTCCCAGACAGACCTGAAGCTGGTGGCCCA
  CCCCCGCGCCAAGAGCAAGGTGTGGAAGTATTTCGGCTTCGACACCAACGCCGAGGGATG
  CATCCTGCAGTGGAAGAAAATCTACTGCCGCATCTGCATGGCCCAGATCGCCTACTCCGG
  AAACACCTCCAACCTGTCCTACCACCTGGAGAAGAACCACCCCGAGGAATTCTGCGAGTT
  CGTCAAGAGCAACACGGAGCAGATGCGTGAAGCCTTCGCCACCGCCTTCTCCAAGCTGAA
  GCCCGAGTCGTCCCAGCAGCCCGGGCAGGACGCGCTGGCCGTCAAGGCCGGCCACGGCTA
  CGACAGCAAGAAGCAGCAGGAGCTGACGGCCGCCGTGCTGGGCCTCATCTGCGAGGGGCT
  GTACCCAGCCTCCATCGTGGACGAGCCCACCTTCAAGGTGCTGCTGAAGACGGCCGACCC
  CCGGTATGAGCTGCCCAGCCGGAAGTACATCTCTACCAAGGCCATCCCTGAGAAGTACGG
  NGCCGTCCGGGAGGTGATCCTGAANGAGCTGGCCGANGCCACCCTGGTGTGGCATCTCCA
  CCGACATGTGGAGGAGTGAGAATCAGAACCGCGCCTACGTCACGCTGGCCGCCCACTTCC
  TGGGCCTGNGCGCCCCCCACTGCCTGTCCATGGGCTTCCGCTGCCTGAAGACCTTCGAGT
  GCCCGAAGAGAAACACGCGAGACCTCACGCGAGTGCTCTATGAGTCCTCATCGAGTGGGC
  ATCAGCGCAAGTCTCCGNGCCACACCACTATGCAGGACATGGAAGCTGCTCCTGCTGAG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004729 unedited
  GAACCGCGGCCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTGAGAGATCTCGCTC
  TTGTCGCCCAGGCTGGAGTGCAGCGATCTCAGCTTACTGCAACCTCCACCTCCCAGGTTC
  AAGCGATTCTCTGGCCTCAGCCTCCAGAAGCTGGGATTACAGGCACATGCCATCAGTCCT
  GGCTAATTTTTGTATTTTTAGTAGAGACGGGGTTTCGCCATGTTGGCCAGGCTGGTCTCG
  ATCTCCTGACCTCAGCTGATCTGCCCACCTCGGCCTCCCAAAGTGCTGCGATTATAGACA
  GGAGCCACCGCCCCCGACCCTCTCTCACTTCTCGAATCTCTTTCCTTTTTCCACCTTCTA
  GGTGTCAAAGACAGTGGATGGTCTCTGAGGTTCAAAACCAAGCTGACCGGGTAAGTATTT
  ACAGCAAAGCATCCAATGGGCTGCTGCGGGGATGACTTGTAAGACAACACGCTTCCTCGC
  TACAGGAAGCTGCTGTCCCTAATGCCAAAGAAACCGCCGCTGACGCCATCCCCCAAGGAG
  AACACCTGCTCCTGGTCCAGGCCCCACTCCCCCTCGTCCTGGTCCTCGGGTTCCGCCTCT
  GCCCCACTCCGGGCGTTTTCATACAGAAACACCTGCTCGTTCACGTGCGCCGGAGCCCAC
  CCGTTCCCTCTTGGCCCTGACCACCCTCGCCGCGGATCCCCAACAAACCCTCACGGGCCA
  CCCCCCCTGGCCGCCCACCCACCAAATTCCTTCGCCGTCCCTTGGGCTACAAGGGGATAA
  AAGGCCCCTCCGGGCCCAACCCCACTCTAGAGGGTACTTCCGTGTGCCCCCTACCCTCCT
  TCGGCACTAGAACCTCCTATCGTCTCCCCCCCCTGTACTTCTCCTCTTTCCAGCTCCTAA
  CCCTCGCCTCCCACCCCAAACATCCGAGCACGTACGCGACATCAATCAGCGC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004729
• Insert Size: 2680 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004729.3, NP_004720.1
• RefSeq Size: 4525 bp
• RefSeq ORF: 2085 bp
• Locus ID: 9189
• UniProt ID: O96006
• Cytogenetics: X;Y
• Domains: zf-BED
• Protein Families: Druggable Genome
• Gene Summary: This gene is located in the pseudoautosomal region 1 (PAR1) of X and Y chromosomes. It was earlier identified as a gene with similarity to Ac transposable elements, however, was found not to have transposase activity. Later studies show that this gene product is localized in the nucleus and functions as a transcription factor. It binds to DNA elements found in the promoter regions of several genes related to cell proliferation, such as histone H1, hence may have a role in regulating genes related to cell proliferation. Alternatively spliced transcript variants with different 5' untranslated region have been found for this gene. [provided by RefSeq, Jan 2010]
  Transcript Variant: This variant (2) uses an alternate acceptor splice site at the 3' terminal exon compared to variant 1. Variants 1-3 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.