GPR37L1 (NM_004767) Human Untagged Clone

CAT#: SC117166

GPR37L1 (untagged)-Human G protein-coupled receptor 37 like 1 (GPR37L1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GPR37L1
• Synonyms: ET(B)R-LP-2; ETBR-LP-2; ETBRLP2
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_004767, RT-PCR generated
  CTCATCCAGCCATGCGGTGGCTGTGGCCCCTGGCTGTCTCTCTTGCTGTGATTTTGGCTG
  TGGGGCTAAGCAGGGTCTCTGGGGGTGCCCCCCTGCACCTGGGCAGGCACAGAGCCGAGA
  CCCAGGAGCAGCAGAGCCGATCCAAGAGGGGCACCGAGGATGAGGAGGCCAAGGGCGTGC
  AGCAGTATGTGCCTGAGGAGTGGGCGGAGTACCCCCGGCCCATTCACCCTGCTGGCCTGC
  AGCCAACCAAGCCCTTGGTGGCCACCAGCCCTAACCCCGACAAGGATGGGGGCACCCCAG
  ACAGTGGGCAGGAACTGAGGGGCAATCTGACAGGGGCACCAGGGCAGAGGCTACAGATCC
  AGAACCCCCTGTATCCGGTGACCGAGAGCTCCTACAGTGCCTATGCCATCATGCTTCTGG
  CGCTGGTGGTGTTTGCGGTGGGCATTGTGGGCAACCTGTCGGTCATGTGCATCGTGTGGC
  ACAGCTACTACCTGAAGAGCGCCTGGAACTCCATCCTTGCCAGCCTGGCCCTCTGGGATT
  TTCTGGTCCTCTTTTTCTGCCTCCCTATTGTCATCTTCAACGAGATCACCAAGCAGAGGC
  TACTGGGTGACGTTTCTTGTCGTGCCGTGCCCTTCATGGAGGTCTCCTCTCTGGGAGTCA
  CGACTTTCAGCCTCTGTGCCCTGGGCATTGACCGCTTCCACGTGGCCACCAGCACCCTGC
  CCAAGGTGAGGCCCATCGAGCGGTGCCAATCCATCCTGGCCAAGTTGGCTGTCATCTGGG
  TGGGCTCCATGACGCTGGCTGTGCCTGAGCTCCTGCTGTGGCAGCTGGCACAGGAGCCTG
  CCCCCACCATGGGCACCCTGGACTCATGCATCATGAAACCCTCAGCCAGCCTGCCCGAGT
  CCCTGTATTCACTGGTGATGACCTACCAGAACGCCCGCATGTGGTGGTACTTTGGCTGCT
  ACTTCTGCCTGCCCATCCTCTTCACAGTCACCTGCCAGCTGGTGACATGGCGGGTGCGAG
  GCCCTCCAGGGAGGAAGTCAGAGTGCAGGGCCAGCAAGCACGAGCAGTGTGAGAGCCAGC
  TCAACAGCACCGTGGTGGGCCTGACCGTGGTCTACGCCTTCTGCACCCTCCCAGAGAACG
  TCTGCAACATCGTGGTGGCCTACCTCTCCACCGAGCTGACCCGCCAGACCCTGGACCTCC
  TGGGCCTCATCAACCAGTTCTCCACCTTCTTCAAGGGCGCCATCACCCCAGTGCTGCTCC
  TTTGCATCTGCAGGCCGCTGGGCCAGGCCTTCCTGGACTGCTGCTGCTGCTGCTGCTGTG
  AGGAGTGCGGCGGGGCTTCGGAGGCCTCTGCTGCCAATGGGTCGGACAACAAGCTCAAGA
  CCGAGGTGTCCTCTTCCATCTACTTCCACAAGCCCAGGGAGTCACCCCCACTCCTGCCCC
  TGGGCACACCTTGCTGAGGCCCCAGTAGGGGTGGGGAGGG
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004767 unedited
  GCAACTTGTTNNGTTAACNATTTCTATAGGCGGCCGCCAGTGTGATGGATATCTGCAGAA
  TTCGGCTTCTCATCCAGCCATGCGGTGGCTGTGGCCCCTGGCTGTCTCTCTTGCTGTGAT
  TTTGGCTGTGGGGCTAAGCAGGGTCTCTGGGGGTGCCCCCCTGCACCTGGGCAGGCACAG
  AGCCGAGACCCAGGAGCAGCAGAGCCGATCCAAGAGGGGCACCGAGGATGAGGAGGCCAA
  GGGCGTGCAGCAGTATGTGCCTGAGGAGTGGGCGGAGTACCCCCGGCCCATTCACCCTGC
  TGGCCTGCAGCCAACCAAGCCCTTGGTGGCCACCAGCCCTAACCCCGACAAGGATGGGGG
  CACCCCAGACAGTGGGCAGGAACTGAGGGGCAATCTGACAGGGGCACCAGGGCAGAGGCT
  ACAGATCCAGAACCCCCTGTATCCGGTGACCGAGAGCTCCTACAGTGCCTATGCCATCAT
  GCTTCTGGCGCTGGTGGTGTTTGCGGTGGGCATTGTGGGCAACCTGTCGGTCATGTGCAT
  CGTGTGGCACAGCTACTACCTGAAGAGCGCCTGGAACTCCATCCTTGCCAGCCTGGCCCT
  CTGGGATTTTCTGGTCCTCTTTTTCTGCCTCCCTATTGTCATCTTCAACGAGATCACCAA
  GCAGAGGCTACTGGGTGACGTTTCTTGTCGTGCCGTGCCCTTCATGGAGGTCTCCTCTCT
  GGGAGTCACGACTTTCAGCCTCTGTGCCCTGNGCATTGACCGCTTNCACGTGGCCACCAG
  CACCCTGCCCAAGGTGAGGCCCATCGAGCGGTGCCAATCCATCCTGNNNCAAGTTGGCTG
  TATCCTGGGGTGGGCTCATGACGCTGGCTGTGCCTGAGCTCCTGCTGTGGCAGCTGGCAC
  AGNAGCCTGCCNCCACATGGGCACCCTGN
  
• Restriction Sites: Please inquire
• ACCN: NM_004767
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004767.2, NP_004758.2
• RefSeq Size: 2516 bp
• RefSeq ORF: 1446 bp
• Locus ID: 9283
• UniProt ID: O60883
• Cytogenetics: 1q32.1
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Gene Summary: G-protein coupled receptor (PubMed:27072655). Has been shown to bind the neuroprotective and glioprotective factor prosaposin (PSAP), leading to endocytosis followed by an ERK phosphorylation cascade (PubMed:23690594). However, other studies have shown that prosaposin does not increase activity (PubMed:27072655, PubMed:28688853). It has been suggested that GPR37L1 is a constitutively active receptor which signals through the guanine nucleotide-binding protein G(s) subunit alpha (PubMed:27072655). Participates in the regulation of postnatal cerebellar development by modulating the Shh pathway (By similarity). Regulates baseline blood pressure in females and protects against cardiovascular stress in males (By similarity). Mediates inhibition of astrocyte glutamate transporters and reduction in neuronal N-methyl-D-aspartate receptor activity (By similarity).[UniProtKB/Swiss-Prot Function]