MAPKAP Kinase 2 (MAPKAPK2) (NM_032960) Human Untagged Clone

CAT#: SC117159

MAPKAPK2 (untagged)-Human mitogen-activated protein kinase-activated protein kinase 2 (MAPKAPK2), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MAPKAP Kinase 2
• Synonyms: MAPKAP-K2; MK-2; MK2
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_032960 edited
  ATGCTGTCCAACTCCCAGGGCCAGAGCCCGCCGGTGCCGTTCCCCGCCCCGGCCCCGCCG
  CCGCAGCCCCCCACCCCTGCCCTGCCGCACCCCCCGGCGCAGCCGCCGCCGCCGCCCCCG
  CAGCAGTTCCCGCAGTTCCACGTCAAGTCCGGCCTGCAGATCAAGAAGAACGCCATCATC
  GATGACTACAAGGTCACCAGCCAGGTCCTGGGGCTGGGCATCAACGGCAAAGTTTTGCAG
  ATCTTCAACAAGAGGACCCAGGAGAAATTCGCCCTCAAAATGCTTCAGGACTGCCCCAAG
  GCCCGCAGGGAGGTGGAGCTGCACTGGCGGGCCTCCCAGTGCCCGCACATCGTACGGATC
  GTGGATGTGTACGAGAATCTGTACGCAGGGAGGAAGTGCCTGCTGATTGTCATGGAATGT
  TTGGACGGTGGAGAACTCTTTAGCCGAATCCAGGATCGAGGAGACCAGGCATTCACAGAA
  AGAGAAGCATCCGAAATCATGAAGAGCATCGGTGAGGCCATCCAGTATCTGCATTCAATC
  AACATTGCCCATCGGGATGTCAAGCCTGAGAATCTCTTATACACCTCCAAAAGGCCCAAC
  GCCATCCTGAAACTCACTGACTTTGGCTTTGCCAAGGAAACCACCAGCCACAACTCTTTG
  ACCACTCCTTGTTATACACCGTACTATGTGGCTCCAGAAGTGCTGGGTCCAGAGAAGTAT
  GACAAGTCCTGTGACATGTGGTCCCTGGGTGTCATCATGTACATCCTGCTGTGTGGGTAT
  CCCCCCTTCTACTCCAACCACGGCCTTGCCATCTCTCCGGGCATGAAGACTCGCATCCGA
  ATGGGCCAGTATGAATTTCCCAACCCAGAATGGTCAGAAGTATCAGAGGAAGTGAAGATG
  CTCATTCGGAATCTGCTGAAAACAGAGCCCACCCAGAGAATGACCATCACCGAGTTTATG
  AACCACCCTTGGATCATGCAATCAACAAAGGTCCCTCAAACCCCACTGCACACCAGCCGG
  GTCCTGAAGGAGGACAAGGAGCGGTGGGAGGATGTCAAGGAGGGAGATGACCAGTGCCTT
  GGCCACAATGCGCGTTGACTACGAGCAGATCAAGATAAAAAAGATTGAAGATGCATCCAA
  CCCTCTGCTGCTGAAGAGGCGGGAAGAAAGCTCGGGCCCTGGAGGCTGCGGCTCTGGCCC
  ACTGAGCCACCGCGCCCTCCTGCCCACGGGAGGACAAGCAATAACTCTCTACA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_032960 unedited
  TTATTACCCCCGCCCGTTGCCGCAAAGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATA
  AGCAGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCC
  GCGAATTCGGCACGAGGCCCGGAGCCGGAGGAGGGGGCGGCCGCGGGCACCCCCGCCTGT
  GCCCCGGCGTCCCACGGCACCATGCTGTCCAACTCCCAGGGCCAGAGCCCGCCGGTGCCG
  TTCCCCGCCCCGGCCCCGCCGCCGCAGCCCCCCACCCCTGCCCTGCCGCACCCCCCGGCG
  CAGCCGCCGCCGCCGCCCCCGCAGCAGTTCCCGCACTTCCCCGTCAAGTCCGGCCTGCCC
  ATCAAGAATAACGCCACTCTCGATGACTACAACGTCACCAGCCAAGTCCTGGGGCTGGGC
  ATCAACGGCCAAGTTTTGCGGATCTTCAACCAAAGGACCCAGCACAAACTCGCCCTCAAA
  ATGCTTCAGGACTTGCCCCAAGCCCACACGGAGGTGGAGCTGCACTGGGCGGCCTTCCAC
  TGCCCGGACAACTGACCGATCCTAGATGTGTACAACCAATCCGTCCCCAGGGAGAAAGAC
  CTTGTGATAGACCATGAATTTTTGGAATGTCGAGAACTCCTTAACCCGAATCCCTATTCT
  AGGTGACCCAGTGTATTCCATACAGAGAACCTTCCTGAATTCTGACGAAACACTGTTGAG
  CGCACACAAGATGTAGCTTAATGATATTTGCTGACCCGGAGGCGGCAACCTGGAAACCCC
  TTATTCCGCTCCCAACGAGGCACCCTCCCGGCACAACGCCCACTGACTATGGGCTCNGCC
  CTGGAGAGACCCGCACCCTTGAAGATGAGGGCTTCCCCTCGTAAGACACCTCCTTNTGTG
  CCCGTTAATGGCGTGACTACATACAG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_032960 unedited
  NAAATAGCTTGNACCGCGCCGCTTTCTANNGATCGGTTTTTTTTTTTTTTTTTTGTAACT
  ATTAAATCTGTTTAGTAAAGACAATTTCGCTCATACAAAACAACAGTTTACAACAATCGA
  GTGCTGGACACACCATCTGAGACAAGGGAGGCGCTGCAGGCACCCAGCGCCTTCCCCCAT
  ACACAAAGACGACCCTGGGCGCCCCTCCACCACCTGCCCCTGAGGGAGAGGAAAGGTGAA
  GTATTCCTGCCACCTCTCCCTCCAAGGGAAGGGGGAGTGGGGGGCCATGGCACAGACAGC
  CTACCCCAGCTGCTCCTCATCTGTGCTGGGGGAGGCCCCTCCCCTCACCGGCCCCGAAGC
  TGAGTTGTTTGGGGTGATGGCAAATGGCTGATGGGCAAGGGGGCAAAGTCAGGACATCTT
  TGGTGCAAAGACTCCCCCTGACCCACCAGGCCACACAATGGAAATCTGAGGGAAGACTTC
  AAAAGTGGCCCTGCCTTTGCCCACAAGGGGTAGCTGGCTGATGCCCCAGAACCCAGGACA
  AACACAGGAAATGCAAAAGCTCTGCCAACAGAAGCTGCCCAGGCTCACCTACATGAGCTG
  GGCGGTGGCCCATGGAGAAATGGCTAAAAATTTAAGGAAAATAAACAAAAATGGGTTAAT
  AATGGCAAGAAGGGGAAGTAGAGAGGTCATTCAGTAGATGAGTGCCTCAGAGGACCTAGG
  ACCACCTTTNAATTGGGGGGAGGGGGGGCCCTGGGGGCTGGGGGTCCAGTCTTCCTGATC
  GCAGTGCCAGTGCCCGGTCACTCCTCTCTGCCTCCCGGAACCCAGCTAATAGAGACAGGC
  CACAGAGGGCCTGGTTCAGAATCTGGGTGGGCTTCCCANGCACTTTGGCAGGCTTCATAT
  GGGCAGGCCAGACCCAAGAAGGGAGAAGTTTCTAC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_032960
• Insert Size: 9000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_032960.2, NP_116584.2
• RefSeq Size: 3071 bp
• RefSeq ORF: 1203 bp
• Locus ID: 9261
• UniProt ID: P49137
• Cytogenetics: 1q32.1
• Domains: pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: MAPK signaling pathway, Neurotrophin signaling pathway, VEGF signaling pathway
• Gene Summary: This gene encodes a member of the Ser/Thr protein kinase family. This kinase is regulated through direct phosphorylation by p38 MAP kinase. In conjunction with p38 MAP kinase, this kinase is known to be involved in many cellular processes including stress and inflammatory responses, nuclear export, gene expression regulation and cell proliferation. Heat shock protein HSP27 was shown to be one of the substrates of this kinase in vivo. Two transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (2) uses an alternate splice junction at the 5' end of the last exon compared to variant 1. The resulting isoform (2) has a longer and distinct C-terminus compared to isoform 1.