Aquaporin 3 (AQP3) (NM_004925) Human Untagged Clone

CAT#: SC117030

AQP3 (untagged)-Human aquaporin 3 (Gill blood group) (AQP3)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Aquaporin 3
• Synonyms: AQP-3; GIL
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC117030 sequence for NM_004925 edited (data generated by NextGen Sequencing)
  ATGGGTCGACAGAAGGAGCTGGTGTCCCGCTGCGGGGAGATGCTCCACATCCGCTACCGG
  CTGCTCCGACAGGCGCTGGCCGAGTGCCTGGGGACCCTCATCCTGGTGATGTTTGGCTGT
  GGCTCCGTGGCCCAGGTTGTGCTCAGCCGGGGCACCCACGGTGGTTTCCTCACCATCAAC
  CTGGCCTTTGGCTTTGCTGTCACTCTGGGCATCCTCATCGCTGGCCAGGTCTCTGGGGCC
  CACCTGAACCCTGCCGTGACCTTTGCCATGTGCTTCCTGGCTCGTGAGCCCTGGATCAAG
  CTGCCCATCTACACCCTGGCACAGACGCTGGGAGCCTTCTTGGGTGCTGGAATAGTTTTT
  GGGCTGTATTATGATGCAATCTGGCACTTCGCCGACAACCAGCTTTTTGTTTCGGGCCCC
  AATGGCACAGCCGGCATCTTTGCTACCTACCCCTCTGGACACTTGGATATGATCAATGGC
  TTCTTTGACCAGTTCATAGGCACAGCCTCCCTTATCGTGTGTGTGCTGGCCATTGTTGAC
  CCCTACAACAACCCCGTCCCCCGAGGCCTGGAGGCCTTCACCGTGGGCCTGGTGGTCCTG
  GTCATTGGCACCTCCATGGGCTTCAACTCCGGCTATGCCGTCAACCCTGCCCGGGACTTT
  GGCCCCCGCCTTTTTACAGCCCTTGCGGGCTGGGGCTCTGCAGTCTTCACGACCGGCCAG
  CATTGGTGGTGGGTGCCCATCGTGTCCCCACTCCTGGGCTCCATTGCGGGTGTCTTCGTG
  TACCAGCTGATGATCGGCTGCCACCTGGAGCAGCCCCCACCCTCCAACGAGGAAGAGAAT
  GTGAAGCTGGCCCATGTGAAGCACAAGGAGCAGATCTGA
  
  Clone variation with respect to NM_004925.4
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004925 unedited
  GCAGCATATTTGTAATACGACTTACGTATAGGGCGGCCGCGAATTCGCACCAGCCTCCCG
  CCACCCCTGCCCGCCCGACAGCGCCGCCGCCTGCCCCGCCATGGGTCGACAGAAGGAGCT
  GGTGTCCCGCTGCGGGGAGATGCTCCACATCCGCTACCGGCTGCTCCGACAGGCGCTGGC
  CGAGTGCCTGGGGACCCTCATCCTGGTGATGTTTGGCTGTGGCTCCGTGGCCCAGGTTGT
  GCTCAGCCGGGGCACCCACGGTGGTTTCCTCACCATCAACCTGGCCTTTGGCTTTGCTGT
  CACTCTGGGCATCCTCATCGCTGGCCAGGTCTCTGGGGCCCACCTGAACCCTGCCGTGAC
  CTTTGCCATGTGCTTCCTGGCTCGTGAGCCCTGGATCAAGCTGCCCATCTACACCCTGGC
  ACAGACGCTGGGAGCCTTCTTGGGTGCTGGAATAGTTTTTGGGCTGTATTATGATGCAAT
  CTGGCACTTCGCCGACAACCAGCTTTTTGTTTCGGGCCCCAATGGCACAGCCGGCATCTT
  TGCTACCTACCCCTCTGGACACTTGGATATGATCAATGGCTTCTTTGACCAGTTCATAGG
  CACAGCCTCCCTTATCGTGTGTGTGCTGGCCATTGTTGACCCCTACAACAACCCCGTCCC
  CCGAGGCCTGGAGGCCTTCACCGTGGGCCTGGTGGTCCTGGTCATTGGCACCTCCATGGG
  CTTCAACTCCGGCTATGCCGTCAACCCTGCCCGGGACTTTGGCCCCCGCCTTTTTACAGC
  CCTTGCGGGCTGGGGCTCTGCAGTCTTCACGACCGGCCAGCATTGGTGGTGGGTGCCCAT
  CGTGNTCCCATCCTGGGCTCATTGCGGNTGTCCTCGTGTCCAACTGATGATCGGCTGCCA
  CCTGNAGCAGCCCCACCTCCACGAGAAGAAATGTGAGCTGGCCATGTGAGCCAA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004925 unedited
  NCGTCTTTATGNACCGCGCCGCATTCTANGATCGATTTTTTTTTTTTTTTTTTAGGCACA
  TATTTATTTTATTTTTTTTAATATAAAAGTAAAAGAGTACATTGGTTGAGTAGAGGATTA
  AAGGAGTGACGACCCTTTCTAAAGTGGGGTCTCCCATCCCGGATCCCTAAGACTGTAACA
  TCTGCTACATACATTAAAAACAAAACAAAACAAAAGCAAACATGAAACTTATGACCTGAC
  TTCACTCCACCCTTCATGCCTGCATTATGACAGAAACACGTCCCACTGCTCCTACTTATG
  TATGTACATCCAGAGCTCCAAACCTAAGCTGTGGCCCCCTCCTCCCAGCCCTACCCACAT
  CCACCCTACTTCCCAAAAGCCTGTAGAAGCCCCCACTTAGAAAAAAAGGCAGACATACAC
  ATACACAGAAACACATATCTATCTGCAGCTCCTCCATGTGAAGCCCCTGAAACATACACA
  CCCTGGAACTTTCCTCTCCTTATGCACAGATGGACAGGCTGCCTTCCCCTGTCTCTGGGC
  TCCCCCAATAGCCAGAATCCCTTCCGACTGGTCCCTTGCCCTGAATATCTGGGAACCCCC
  CCCCCAACACACACCCCTGCACACACATGCACACACATGCACACACATGCACACACACAC
  ATACCTGCTGCCCATTCTCTTTCCCTTGGGAGACAAAGGATGTATTGACCCAAATTCCGT
  TCCACCCAGCTTAGGGCAGTGGCCTAAGGTGCTATTTGGGCAAAGTCCAGTGGAAATCCT
  GAAGGGGCTGTCTCGTGGGGTGAGGGTAGATAGGGAGCTCCTTACCCGAAAAGGTGGATC
  GTGAAGGGGCCTCTTGGGNAGTGGCCCTG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004925
• Insert Size: 2000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004925.3, NP_004916.1
• RefSeq Size: 1835 bp
• RefSeq ORF: 879 bp
• Locus ID: 360
• UniProt ID: Q92482
• Cytogenetics: 9p13.3
• Domains: MIP
• Protein Families: Druggable Genome, Transmembrane
• Gene Summary: This gene encodes the water channel protein aquaporin 3. Aquaporins are a family of small integral membrane proteins related to the major intrinsic protein, also known as aquaporin 0. Aquaporin 3 is localized at the basal lateral membranes of collecting duct cells in the kidney. In addition to its water channel function, aquaporin 3 has been found to facilitate the transport of nonionic small solutes such as urea and glycerol, but to a smaller degree. It has been suggested that water channels can be functionally heterogeneous and possess water and solute permeation mechanisms. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2015]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).