KCNJ8 (NM_004982) Human Untagged Clone

CAT#: SC117022

KCNJ8 (untagged)-Human potassium inwardly-rectifying channel, subfamily J, member 8 (KCNJ8)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: KCNJ8
• Synonyms: KIR6.1; uKATP-1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC117022 sequence for NM_004982 edited (data generated by NextGen Sequencing)
  ATGTTGGCCAGAAAGAGTATCATCCCGGAGGAGTATGTGCTGGCGCGCATCGCCGCAGAG
  AACCTGCGCAAGCCGCGCATCCGAGACCGCCTCCCCAAAGCCCGCTTCATCGCCAAGAGC
  GGGGCCTGCAACCTGGCGCATAAGAACATCCGTGAGCAAGGACGCTTTCTACAGGACATC
  TTCACCACCTTGGTGGACCTGAAATGGCGCCACACGCTGGTCATCTTTACCATGTCCTTC
  CTCTGCAGCTGGCTGCTCTTCGCTATCATGTGGTGGCTGGTGGCCTTTGCCCATGGGGAC
  ATCTATGCTTACATGGAGAAAAGTGGAATGGAGAAAAGTGGTTTGGAGTCCACTGTGTGT
  GTGACTAATGTCAGGTCTTTCACTTCTGCTTTTCTCTTCTCCATTGAAGTTCAAGTTACC
  ATTGGGTTTGGAGGGAGGATGATGACAGAGGAATGCCCTTTGGCCATCACGGTTTTGATT
  CTCCAGAATATTGTGGGTTTGATCATCAATGCAGTCATGTTAGGCTGCATTTTCATGAAA
  ACAGCTCAGGCTCACAGAAGGGCAGAAACTTTGATTTTCAGCCGCCATGCTGTGATTGCC
  GTCCGAAATGGCAAGCTGTGCTTCATGTTCCGAGTGGGTGACCTGAGGAAAAGCATGATC
  ATTAGTGCCTCTGTGCGCATCCAGGTGGTCAAGAAAACAACTACACCTGAAGGGGAGGTG
  GTTCCTATTCACCAACTGGACATTCCTGTTGATAACCCAATCGAGAGCAATAACATTTTT
  CTGGTGGCCCCTTTGATCATCTGCCACGTGATTGACAAGCGCAGTCCCCTGTATGACATC
  TCAGCAACTGACCTGGCCAACCAAGACTTGGAGGTCATAGTTATTCTGGAAGGAGTGGTT
  GAAACTACTGGCATCACCACACAAGCACGAACCTCCTACATTGCTGAGGAGATCCAATGG
  GGCCACCGCTTTGTGTCCATTGTGACTGAGGAAGAAGGAGTGTATTCTGTGGATTACTCC
  AAATTTGGCAACACTGTTAAAGTAGCTGCTCCACGGTGCAGTGCCCGAGAGCTGGATGAG
  AAACCTTCCATCCTTATTCAGACCCTCCAAAAGAGTGAACTGTCTCATCAAAATTCTCTG
  AGGAAGCGCAACTCCATGAGAAGAAACAATTCCATGAGGAGGAACAATTCTATCCGAAGG
  AACAATTCTTCCCTCATGGTACCAAAGGTGCAATTTATGACTCCAGAAGGAAATCAAAAC
  ACATCGGAATCATGA
  
  Clone variation with respect to NM_004982.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004982 unedited
  GTTAAATTTGTATACGACTACTATAGGGCGGCCGCGAATTCGCACGAGGCGGCCCCTGGT
  CCCGCGCGCCAGCATGGGAGAGCGAGGGACCTGCCCGCGGCCCGCCGGCGTGTGCAAGGA
  GGTCCAGCCGCCGCGCCCGCTACCCGGAGTCTGAGGACGGGTGTCCAGGGACGGAGAGGC
  AGGTGAGAGGGAGGTGGCTAAGCTGGCTATGGTGACAGGACGATGTTGGCCAGAAAGAGT
  ATCATCCCGGAGGAGTATGTGCTGGCGCGCATCGCCGCAGAGAACCTGCGCAAGCCGCGC
  ATCCGAGACCGCCTCCCCAAAGCCCGCTTCATCGCCAAGAGCGGGGCCTGCAACCTGGCG
  CATAAGAACATCCGTGAGCAAGGACGCTTTCTACAGGACATCTTCACCACCTTGGTGGAC
  CTGAAATGGCGCCACACGCTGGTCATCTTTACCATGTCCTTCCTCTGCAGCTGGCTGCTC
  TTCGCTATCATGTGGTGGCTGGTGGCCTTTGCCCATGGGGACATCTATGCTTACATGGAG
  AAAAGTGGAATGAGAAAAGTGGTTTGGAGTCCACTGTGTGTGTGACTAATGTCAGGTCTT
  TCACTTCTGCTTTTCTCTTCTCATTGAAGTTCAAGTTCCATTGNGTTTGGANGGGAGGAT
  GATGACAGANGAATGCCCTTTGGCATTNACCGGNTTTGATTCTCAATATTTTGGGNGGGG
  TTTTGATCATCAATGCAATCATGTTTAGGCTGCATTTTCATGAAACCCAGCTCAGGCTTA
  ACAGAAGGGCAGAACTTTTTTGATTTTCAGCGCCATGCTTGGNTAATTTTCCGTCCGAAT
  GGNAAAGCTGTGGTTTCATGTTTTTCCCGCGGGGGGGGGGGACCCCGGGGGAAAACATGA
  TCCTTTTTGGGCCCCCTGTCCATCCCCCCGGGGGGGCAGAAAA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004982 unedited
  GCTATGGACCGGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTGGTGTGTTA
  CTTTTTATTACTACAGAAAGTGCTGTTGCTTGAATATGAATATCATTTAGTGTAATAAAA
  TGTAGAAGGACACATTATTGTGTTCCAGCTCTGGTTCAGTCTGGCAGTGCCCAGCATAAA
  CCGTCAAAACTTGATAAAAGACTGTCTTGGGGTTATCTTGCTGTCATGATTCCGATGTGT
  TTTGATTTCCTTCTGGAGTCATAAATTGCACCTTTGGTACCATGAGGGAAGAATTGTTCC
  TTCGGATAGAATTGTTCCTCCTCATGGAATTGTTTCTTCTCATGGAGTTGCGCTTCCTCA
  GAGAATTTTGATGAGACAGTTCACTCTTTTGGAGGGTCTGAATAAGGATGGAAGGTTTCT
  CATCCAGCTCTCGGGCACTGCACCGTGGAGCAGCTACTTTAACAGTGTTGCCAAATTTGG
  AGTAATCCACAGAATACACTCCTTCTTCCTCAGTCACAATGGACACAAAGCGGTGGCCCC
  ATTGGATCTCCTCACAATGTAAGAGGTTCGTGCTTGTGTGGTGATGCCAGTAGTTTCAAC
  CACTCCTTCCAGATAACTATGACCTCCAAGTCTTGGTTGGCCAGGTCAGTTGCTGAGATG
  TCATACAGGNGGACTGCGCTTGTCAATCACGTGGCAGATGATCAAAGGGGCCACCAGAAA
  AATGTTATTGCTCTCGATTGGGTTATCAACAGGATGTCCAGTTGGTGAATANGAACCACC
  TCCNCTTCAGTGGTAGNTGNTTTCTTGACCACCTGGATGCGCACCAGAGCACTATGATCA
  TGCTTTTTCCTCAGNCACCCACTCGACATGAAGCACAGCTGCCATTTTCGACGCATCACA
  CATGGCGCTGAAATCAAGTTNCTGNCCTCTGTGAGCCTGACTGTTCATGAAATGCAGCTA
  CTGACGCATGATGA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004982
• Insert Size: 1660 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004982.2, NP_004973.1
• RefSeq Size: 2381 bp
• RefSeq ORF: 1275 bp
• Locus ID: 3764
• UniProt ID: Q15842
• Cytogenetics: 12p12.1
• Domains: IRK
• Protein Families: Druggable Genome, Ion Channels: Potassium, Transmembrane
• Gene Summary: Potassium channels are present in most mammalian cells, where they participate in a wide range of physiologic responses. The protein encoded by this gene is an integral membrane protein and inward-rectifier type potassium channel. The encoded protein, which has a greater tendency to allow potassium to flow into a cell rather than out of a cell, is controlled by G-proteins. Defects in this gene may be a cause of J-wave syndromes and sudden infant death syndrome (SIDS). [provided by RefSeq, May 2012]