FEZ1 (NM_005103) Human Untagged Clone

CAT#: SC116927

FEZ1 (untagged)-Human fasciculation and elongation protein zeta 1 (zygin I) (FEZ1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: FEZ1
• Synonyms: UNC-76
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC116927 sequence for NM_005103 edited (data generated by NextGen Sequencing)
  ATGGAGGCCCCACTGGTGAGTCTGGATGAAGAGTTTGAGGACCTTCGACCCTCCTGCTCG
  GAGGACCCGGAGGAGAAGCCCCAGTGTTTCTATGGTTCATCTCCCCACCATCTCGAGGAC
  CCCTCCCTCTCCGAGCTTGAGAATTTTTCTTCCGAAATAATCAGCTTCAAGTCCATGGAG
  GACCTCGTAAATGAATTTGATGAGAAGCTCAATGTCTGCTTTCGGAACTACAACGCCAAG
  ACCGAGAACCTAGCTCCCGTGAAGAACCAGTTACAGATCCAAGAGGAGGAGGAGACCCTT
  CAGGACGAGGAGGTTTGGGATGCTCTGACAGACAATTACATCCCTTCACTCTCAGAAGAC
  TGGAGGGATCCAAACATCGAGGCTCTGAATGGCAACTGCTCTGACACTGAGATCCATGAG
  AAAGAAGAGGAAGAGTTCAATGAGAAGAGTGAAAATGATTCCGGTATCAACGAGGAGCCT
  CTGCTCACAGCAGATCAGGTAATTGAGGAGATTGAGGAAATGATGCAGAACTCCCCAGAC
  CCTGAGGAAGAAGAGGAGGTTCTGGAAGAAGAGGATGGAGGAGAAACTTCCTCCCAGGCA
  GACTCGGTCCTCCTGCAGGAGATGCAGGCATTGACACAGACCTTCAACAACAACTGGTCC
  TATGAAGGGCTGAGGCACATGTCTGGGTCTGAGCTGACCGAGCTGCTGGACCAGGTGGAG
  GGTGCCATCCGTGACTTCTCGGAGGAGCTGGTGCAGCAGCTGGCCCGCCGGGACGAGCTG
  GAGTTTGAGAAGGAAGTGAAGAACTCCTTTATCACGGTGCTTATTGAGGTTCAGAACAAG
  CAGAAGGAGCAGCGAGAACTGATGAAAAAGAGGCGGAAAGAGAAAGGGCTGAGCCTGCAG
  AGCAGCCGGATAGAGAAGGGAAACCAGATGCCTCTCAAGCGCTTCAGCATGGAAGGCATC
  TCCAACATTCTGCAGAGTGGCATCCGCCAGACCTTTGGCTCCTCAGGAACTGACAAACAG
  TATCTGAACACAGTCATTCCTTACGAGAAGAAAGCCTCTCCTCCCTCAGTGGAAGACCTG
  CAGATGCTGACAAACATTCTCTTTGCCATGAAGGAGGATAATGAGAAGGTGCCTACTTNG
  CTAACGGACTACATTTTAAAAGTGCTCTGCCCTACCTAA
  
  Clone variation with respect to NM_005103.4
  1139 t=>n
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005103 unedited
  GTATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCTCCCTCCGCCAGGACC
  CGCACAGATAAACTCATCCTGAAAGTCGCTGTTGTTCTCCTGCTGAGCAAGAATGGAGGC
  CCCACTGGTGAGTCTGGATGAAGAGTTTGAGGACCTTCGACCCTCCTGCTCGGAGGACCC
  GGAGGAGAAGCCCCAGTGTTTCTATGGTTCATCTCCCCACCATCTCGAGGACCCCTCCCT
  CTCCGAGCTTGAGAATTTTTCTTCCGAAATAATCAGCTTCAAGTCCATGGAGGACCTCGT
  AAATGAATTTGATGAGAAGCTCAATGTCTGCTTTCGGAACTACAACGCCAAGACCGAGAA
  CCTAGCTCCCGTGAAGAACCAGTTACAGATCCAAGAGGAGGAGGAGACCCTTCAGGACGA
  GGAGGTTTGGGATGCTCTGACAGACAATTACATCCCTTCACTCTCAGAAGACTGGAGGGA
  TCCAAACATCGAGGCTCTGAATGGCAACTGCTCTGACACTGAGATCCATGAGAAAGAAGA
  GGAAGAGTTCAATGAGAAGAGTGAAAATGATTCCGGTATCAACGAGGAGCCTCTGCTCAC
  AGCAGATCAGGTAATTGAGGAGATTGAGGAAATGATGCAGAACTCCCCAGACCCTGAGGA
  AGAAGAGGAGGTTCTGGAAGAAGAGGATGGANGGAGAAACTTCCTCCCAGGCAGACTCGG
  TCCTTCTGCANGAGATGCANGCATTGACACAGACCTTCAACAACACTGGTCCTATGAAGG
  GCTGANGCACATGNTCTGGGTCTGAGCTGACCGAGCTGCTGNACCANGTGGGNAGGNTGC
  CCATCCGTGACTTNCTCGAGNAGCTGGTGCANCAACTGGCCCGCCGGGACGAGCTGNAGT
  TNGANAAGGNAAGTGAAAACTNCTTNATCACGTGCTAATTTGGGNTCANAACAGA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005103 unedited
  CGTTCGGGGGGTAATCCAAATTAGTCTTAGNAGTTAAGCTGCTTTTGAGGGCTGTCAGCC
  GACTACATAATGAGCGGTGAAAGCGGCTGCCTTCCCCTCTCCTGACACCAGCAAGGGGGA
  GGCACCATCACCGGCCCTGCCCCATCATGCATCCAATGATTACTAGCACTAGAAGCCAAC
  GGCAAAGGACCCCGCGCGCTTGCTCGTGTTTAATCCAGGTTAAGCTATACACGTTTAAAT
  ACATGTCGGAGGTTACATGGTCTCATGCAGTCCCTGTGATGGAATGACTCTTGCTCATTG
  ACCTCCTGCAGCGAGGCTGCTCCAAAGGGCAAGGTTACGTAAGGCAGAGCACTTTTAAAA
  TGTAGTCCGTTAGCAAGTAGGCACCTTCTCATTATCCTCCTTCATGGCAAAGAGAATGTT
  TGTCACCATCTGCAGGTCTTCCACTGAGGGAGGAGAGGCTTTCTTCTCGTAAGGAATGAC
  TGTGTTCAGATACTGTTTGTCAGTTCCTGAGGAGCCAAAGGTCTGGCGGATGCCACTCTG
  CAGAATGTTGGAGATGCCTTCCATGCTGAAGCGCTTGAGAGGCATCTGGTTTCCCTTCTC
  TATCCGGCTGCTCTGCATGCTCAGTCCTTTCTCTATCCGACTCTTTTTCATCAGTTATCG
  CTGCTCCTTCTGCTTGTTCTGAACCTCAATAAGCACCGTGATAAAGGAGGTGTTCACTTN
  CTTCTGAAACTGCAACTCGTNCCGGCGGGCCAGCTGCTGCACCAACTACTCCGAGTAGTC
  ACAGATGGCACCCTCCACCTGGTCCATCAGCTCGGTCAGCTAAAACCCAGACATGTGCCC
  TCAGCCCTTCATAAGACCAGTTGTTGTTGAAGGTCTGGGCCATGACTGCATCTCCTGCAG
  GAAGACCCGAACTGCCTGAGAGGAGGTCCTCCCCATCCTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005103
• Insert Size: 1650 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005103.3, NP_005094.1
• RefSeq Size: 1702 bp
• RefSeq ORF: 1179 bp
• Locus ID: 9638
• UniProt ID: Q99689
• Cytogenetics: 11q24.2
• Gene Summary: This gene is an ortholog of the C. elegans unc-76 gene, which is necessary for normal axonal bundling and elongation within axon bundles. Expression of this gene in C. elegans unc-76 mutants can restore to the mutants partial locomotion and axonal fasciculation, suggesting that it also functions in axonal outgrowth. The N-terminal half of the gene product is highly acidic. Alternatively spliced transcript variants encoding different isoforms of this protein have been described. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) encodes the full-length isoform (1), which has a longer C-terminal end compared to isoform 2.