Histone H1.2 (HIST1H1C) (NM_005319) Human Untagged Clone

CAT#: SC116823

HIST1H1C (untagged)-Human histone cluster 1, H1c (HIST1H1C)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Histone H1.2
• Synonyms: H1.2; H1C; H1F2; H1s-1; HIST1H1C
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC116823 sequence for NM_005319 edited (data generated by NextGen Sequencing)
  ATGTCCGAGACTGCTCCTGCCGCTCCCGCTGCCGCGCCTCCTGCGGAGAAGGCCCCTGTA
  AAGAAGAAGGCGGCCAAAAAGGCTGGGGGTACGCCTCGTAAGGCGTCTGGTCCCCCGGTG
  TCAGAGCTCATCACCAAGGCTGTGGCCGCCTCTAAAGAGCGTAGCGGAGTTTCTCTGGCT
  GCTCTGAAAAAAGCGTTGGCTGCCGCCGGCTATGATGTGGAGAAAAACAACAGCCGTATC
  AAACTTGGTCTCAAGAGCCTGGTGAGCAAGGGCACTCTGGTGCAAACGAAAGGCACCGGT
  GCTTCTGGCTCCTTTAAACTCAACAAGAAGGCAGCCTCCGGGGAAGCCAAGCCCAAGGTT
  AAAAAGGCGGGCGGAACCAAACCTAAGAAGCCAGTTGGGGCAGCCAAGAAGCCCAAGAAG
  GCGGCTGGCGGCGCAACTCCGAAGAAGAGCGCTAAGAAAACACCGAAGAAAGCGAAGAAG
  CCGGCCGCGGCCACTGTAACCAAGAAAGTGGCTAAGAGCCCAAAGAAGGCCAAGGTTGCG
  AAGCCCAAGAAAGCTGCCAAAAGTGCTGCTAAGGCTGTGAAGCCCAAGGCCGCTAAGCCC
  AAGGTTGTCAAGCCTAAGAAGGCGGCGCCCAAGAAGAAATAG
  
  Clone variation with respect to NM_005319.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005319 unedited
  GGGGGTTTAAATTTGTATACGACTCATATAGGCGGCCGCGNATTCGGCACGAGGGCCACT
  TGTACCCGAGTTTTTGATTCTCAACATGTCCGAGACTGCTCCTGCCGCTCCCGCTGCCGC
  GCCTCCTGCGGAGAAGGCCCCTGTAAAGAAGAAGGCGGCCAAAAGGCTGGGGGTACGCCT
  CGTAAGGCGTCTGGTCCCCCGGTGTCAGAGCTCATCACCAAGGCTGTGGCCGCCTCTAAA
  GAGCGTAGCGGAGTTTCTCTGGCTGCTCTGAAAAAAGCGTTGGCTGCCGCCGGCTATGAT
  GTGGAGAAAAACAACAGCCGTATCAAACTTGGTCTCAAGAGCCTGGTGAGCAAGGGCACT
  CTGGTGCAAACGAAAGGCACCGGTGCTTCTGGCTCCTTTAAACTCAACAAGAAGGCAGCC
  TCCGGGGAAGCCAAGCCCAAGGTTAAAAAGGCGGGCGGAACCAAACCTAAGAAGCCAGTT
  GGGGCAGCCAAGAAGCCCAAGAAGGCGGCTGGCGGCGCAACTCCGAAGAAGAGCGCTAAG
  AAAACACCGAAGAAAGCGAAGAAGCCGGCCGCGGCCACTGTAACCAAGAAAGTGGCTAAG
  AGCCCAAAGAAGGCCAAGGTTGCGAAGCCCAAGAAAGCTGCCAAAAGTGCTGCTAAGGCT
  GTGAAGCCCAAGGCCGCTAAGCCCAAGGTTGTCAAGCCTAAGAAGGCGGCGCCCAAGAAG
  AAATAGGCGAACGCCTACTTCTAAAACCCAAAGGCTCTTTTCAGAGCCACCACTGATCTC
  AATAAAAGAGCTGGATAATTTCTTTACAAAAAANAAAAAAAAAAACTCGACTCTAGATTT
  GCGCCGCGGTCATAGCTGTTTCCTGAACAGACCCCGGNTGGGATCCCTGTGACCA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005319 unedited
  GGACCGCGGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGTAAAAAAATATCC
  AGCTCTTTTATTGAGATCAGTGGTGGCTCTGAAAAGACCTTTTGGGTTTTAGAAGTAGGC
  GTTCGCCTATTTCTTCTTGGGCGCCGCCTTCTTAGGCTTGACAACCTTGGGCTTAGCGGC
  CTTGGGCTTCACAGCCTTAGCAGCACTTTTGGCAGCTTTCTTGGGCTTCGCAACCTTGGC
  CTTCTTTGGGCTCTTAGCCACTTTCTTGGTTACAGTGGCCGCGGCCGGCTTCTTCGCTTT
  CTTCGGTGTTTTCTTAGCGCTCTTCTTCGGAGTTGCGCCGCCAGCCGCCTTCTTGGGCTT
  CTTGGCTGCCCCAACTGGCTTCTTAGGTTTGGTTCCGCCCGCCTTTTTAACCTTGGGCTT
  GGCTTCCCCGGAGGCTGCCTTCTTGTTGAGTTTAAAGGAGCCAGAAGCACCGGTGCCTTT
  CGTTTGCACCAGAGTGCCCTTGCTCACCAGGCTCTTGAGACCAAGTTTGATACGGCTGTT
  GTTTTTCTCCACATCATAGCCGGCGGCAGCCAACGCTTTTTTCAGAGCAGCCAGAGAAAC
  TCCGCTACGCTCTTTAGAGGCGGCCACAGCCTTGGTGATGAGCTCTGACACCGGGGGACC
  AGACGCCTTACGAGGCGTACCCCCAGCCTTTTTGGCCGCCTTCTTCTTTACAGGGGCCCT
  TCTCCGCAAGAAGCGCCGCAACGGGAGCCGCCAGAGCANTCTCGGACATGTTGAGAACAA
  AAACTCGGGTACAAGTGGCCTTCGTGCCGAATTCCGGCCGCCCTAAATGAAGCCGATACA
  AAATCTGACGGTCACTAACGGACCCTGCTTATTAGACCTCCACCGACCCGCCTACCGCCA
  TTTGGTCACGGGGCGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005319
• Insert Size: 820 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005319.3, NP_005310.1
• RefSeq Size: 732 bp
• RefSeq ORF: 642 bp
• Locus ID: 3006
• UniProt ID: P16403
• Cytogenetics: 6p22.2
• Domains: linker_histone
• Gene Summary: Histones are basic nuclear proteins responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H1 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. [provided by RefSeq, Aug 2015]