GRPR (NM_005314) Human Untagged Clone

CAT#: SC116819

GRPR (untagged)-Human gastrin-releasing peptide receptor (GRPR)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GRPR
• Synonyms: BB2; BB2R; BRS2
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC116819 sequence for NM_005314 edited (data generated by NextGen Sequencing)
  ATGGCTCTAAATGACTGTTTCCTTCTGAACTTGGAGGTGGACCATTTCATGCACTGCAAC
  ATCTCCAGTCACAGTGCGGATCTCCCCGTGAACGATGACTGGTCCCACCCGGGGATCCTC
  TATGTCATCCCTGCAGTTTATGGGGTTATCATTCTGATAGGCCTCATTGGCAACATCACT
  TTGATCAAGATCTTCTGTACAGTCAAGTCCATGCGAAACGTTCCAAACCTGTTCATTTCC
  AGTCTGGCTTTGGGAGACCTGCTCCTCCTAATAACGTGTGCTCCAGTGGATGCCAGCAGG
  TACCTGGCTGACAGATGGCTATTTGGCAGGATTGGCTGCAAACTGATCCCCTTTATACAG
  CTTACCTCTGTTGGGGTGTCTGTCTTCACACTCACGGCGCTCTCGGCAGACAGATACAAA
  GCCATTGTCCGGCCAATGGATATCCAGGCCTCTCATGCCCTGATGAAGATCTGCCTCAAA
  GCCGCCTTTATCTGGATCATCTCCATGCTGCTGGCCATTCCAGAGGCCGTGTTTTCTGAC
  CTCCATCCCTTCCATGAGGAAAGCACCAACCAGACCTTCATTAGCTGTGCCCCATACCCA
  CACTCTAATGAGCTTCACCCCAAAATCCATTCTATGGCTTCCTTTCTGGTCTTCTACGTC
  ATTCCACTGTCGATCATCTCTGTTTACTACTACTTCATTGCTAAAAATCTGATCCAGAGT
  GCTTACAATCTTCCCGTGGAAGGGAATATACATGTCAAGAAGCAGATTGAATCCCGGAAG
  CGACTTGCCAAGACAGTGCTGGTGTTTGTGGGCCTGTTCGCCTTCTGCTGGCTCCCCAAT
  CATGTCATCTACCTGTACCGCTCCTACCACTACTCTGAGGTGGACACCTCCATGCTCCAC
  TTTGTCACCAGCATCTGTGCCCGCCTCCTGGCCTTCACCAACTCCTGCGTGAACCCCTTT
  GCCCTCTACCTGCTGAGCAAGAGTTTCAGGAAACAGTTCAACACTCAGCTGCTCTGTTGC
  CAGCCTGGCCTGATCATCCGGTCTCACAGCACTGGAAGGAGTACAACCTGCATGACCTCC
  CTCAAGAGTACCAACCCCTCCGTGGCCACCTTTAGCCTCATCAATGGAAACATCTGTCAC
  GAGCGGTATGTCTAG
  
  Clone variation with respect to NM_005314.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005314 unedited
  NGGTCAAAATTGTATACGACTCACTATAGGGCGGCCGCGATTCGCCCTTCTTATCTTCAT
  CTTCACTCGGTTGCAAAATCAATAGTTAAGAAATAGCATCTAAGGGAACTTTTAGGTGGN
  GAAAAAAAATCTAGAGATGGCTCTAAATGACTGTTTCCTTCTGAACTTGGAGGTGGACCA
  TTTCATGCACTGCAACATCTCCAGTCACAGTGCGGATCTCCCCGTGAACGATGACTGGTC
  CCACCCGGGGATCCTCTATGTCATCCCTGCAGTTTATGGGGTTATCATTCTGATAGGCCT
  CATTGGCAACATCACTTTGATCAAGATCTTCTGTACAGTCAAGTCCATGCGAAACGTTCC
  AAACCTGTTCATTTCCAGTCTGGCTTTGGGAGACCTGCTCCTCCTAATAACGTGTGCTCC
  AGTGGATGCCAGCAGGTACCTGGCTGACAGATGGCTATTTGGCAGGATTGGCTGCAAACT
  GATCCCCTTTATACAGCTTACCTCTGTTGGGGTGTCTGTCTTCACACTCACGGCGCTCTC
  GGCAGACAGATACAAAGCCATTGTCCGGCCAATGGATATCCAGGCCTCTCATGCCCTGAT
  GAAGATCTGCCTCAAAGCCGCCTTTATCTGGATCATCTCCATGCTGCTGGCCATTCCAGA
  GGCCGTGTTTTCTGACCTCCATCCCTTCCATGAGGAAAGCACCAACCAGACCTTCATTAG
  CTGTGCCCCATACCCACACTCTAATGAGCTTCACCCCAAAATCCATTCTATGGCTTCCTT
  TCTGGTCTTCTACGTCATTCCACTGTCGATCATCTCTGTTTACTACTACTTCATTGCTAA
  AAATCTGATCCAGAGTGCTTACCATCTTCCCGTGGAAGGAATATACATGTCAAGAAGCAG
  ATTGAATCCCNGAAGCGACTTGNNCAGACGTGCTGGTGTTTGTGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005314 unedited
  NNGGGCCATGGGGATGGCAACTTCCAGGGCCAGNAAAGCACTGGGGNAGGGTCACAGGGA
  TGCCACCCGGGATCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGACAA
  GCTTGATATCGGTACCAGATCTGAATTCGCCCTTCACCTACACCACTCAGGAGCATTCTG
  AAGGCTCTTTGGAGGGCACAGACACAACAATGGATGCAAGGGTTCCTGTCTAGCCATAAA
  GCAAAACCGTCCCTCAGGGGGCAAAATCAAGGGTCAATCTAGACATACCGCTCGTGACAG
  ATGTTTCCATTGATGAGGCTAAAGGTGGCCACGGAGGGGTTGGTACTCTTGAGGGAGGTC
  ATGCAGGTTGTACTCCTTCCAGTGCTGTGAGACCGGATGATCAGGCCAGGCTGGCAACAG
  AGCAGCTGAGTGTTGAACTGTTTCCTGAAACTCTTGCTCAGCAGGTAGAGGGCAAAGGGG
  TTCACGCAGGAGTTGGTGAAGGCCAGGAGGCGGGCACAGATGCTGGTGACAAAGTGGAGN
  CATGGAGGTGTCCACCTCAGAGTAGTGGTAGGAGCGGTACAGGTAGATGACATGATTGGG
  GAGCCAGCAGAAGGCGAACAGGCCCACAAACACCAGCACTGTCTTGGCAAGTCGCTTCCG
  GGATTCAATCTGCTTCTTGACATGTATATTCCCTTCCCGGGAAGATTGTAGCACTCTGGA
  TCAGATTTTTAGCAATGAAGTAGTAGTAAACAGAGATGATCGACAGTGAATGACGTAGAA
  GACCAGAAAGGAGCATAGATGGNATTTGGGGGTAAGCTCATAAGTGTGGGGTATGGGCAC
  ATAATGAAGNNGNGGGGGGGGCTTCTCATGAGGGGGTGGGGGGGGAAAAAACCCGCTTTT
  GGAGTCACAN
  
• Restriction Sites: Please inquire
• ACCN: NM_005314
• Insert Size: 1700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005314.2, NP_005305.1
• RefSeq Size: 2681 bp
• RefSeq ORF: 1155 bp
• Locus ID: 2925
• UniProt ID: P30550
• Cytogenetics: Xp22.2
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Calcium signaling pathway, Neuroactive ligand-receptor interaction
• Gene Summary: Gastrin-releasing peptide (GRP) regulates numerous functions of the gastrointestinal and central nervous systems, including release of gastrointestinal hormones, smooth muscle cell contraction, and epithelial cell proliferation and is a potent mitogen for neoplastic tissues. The effects of GRP are mediated through the gastrin-releasing peptide receptor. This receptor is a glycosylated, 7-transmembrane G-protein coupled receptor that activates the phospholipase C signaling pathway. The receptor is aberrantly expressed in numerous cancers such as those of the lung, colon, and prostate. An individual with autism and multiple exostoses was found to have a balanced translocation between chromosome 8 and a chromosome X breakpoint located within the gastrin-releasing peptide receptor gene. [provided by RefSeq, Jul 2008]