IFI16 (NM_005531) Human Untagged Clone

CAT#: SC116712

IFI16 (untagged)-Human interferon, gamma-inducible protein 16 (IFI16), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: IFI16
• Synonyms: IFNGIP1; PYHIN2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_005531 edited
  GAATTCGGCACGAGGGAATAGGAGCAAGCCAGCACTAGTCAGCTAACTAAGTGACTCAAC
  CAAGGCCTTTTTTCCTTGTTATCTTTGCAGATACTTCATTTTCTTAGCGTTTCTGGAGAT
  TACAACATCCTGCGGTTCCGTTTCTGGGAACTTTACTGATTTATCTCCCCCCTCACACAA
  ATAAGCATTGATTCCTGCATTTCTGAAGATCTCAAGATCTGGACTACTGTTGAAAAAATT
  TCCAGTGAGGCTCACTTATGTCTGTAAAGATGGGAAAAAAATACAAGAACATTGTTCTAC
  TAAAAGGATTAGAGGTCATCAATGATTATCATTTTAGAATGGTTAAGTCCTTACTGAGCA
  ACGATTTAAAACTTAATTTAAAAATGAGAGAAGAGTATGACAAAATTCAGATTGCTGACT
  TGATGGAAGAAAAGTTCCGAGGTGATGCTGGTTTGGGCAAACTAATAAAAATTTTCGAAG
  ATATACCAACGCTTGAAGACCTGGCTGAAACTCTTAAAAAAGAAAAGTTAAAAGTAAAAG
  GACCAGCCCTATCAAGAAAGAGGAAGAAGGAAGTGGATGCTACTTCACCTGCACCCTCCA
  CAAGCAGCACTGTCAAAACTGAAGGAGCAGAGGCAACTCCTGGAGCTCAGAAAAGAAAAA
  AATCAACCAAAGAAAAGGCTGGACCCAAAGGGAGTAAGGTGTCCGAGGAACAGACTCAGC
  CTCCCTCTCCTGCAGGAGCCGGCATGTCCACAGCCATGGGCCGTTCCCCATCTCCCAAGA
  CCTCATTGTCAGCTCCACCCAACACTTCTTCAACTGAGAACCCGAAAACAGTGGCCAAAT
  GTCAGGTAACTCCCAGAAGAAATGTTCTCCAAAAACGCCCAGTGATAGTGAAGGTACTGA
  GTACAACAAAGCCATTTGAATATGAGACCCCAGAAATGGAGAAAAAAATAATGTTTCATG
  CTACAGTGGCTACACAGACACAGTTCTTCCATGTGAAGGTTTTAAACACCAGCTTGAAGG
  AGAAATTCAATGGAAAGAAAATCATCATCATATCAGATTATTTGGAATATGATAGTCTCC
  TAGAGGTCAATGAAGAATCTACTGTATCTGAAGCTGGTCCTAACCAAACGTTTGAGGTTC
  CAAATAAAATCATCAACAGAGCAAAGGAAACTCTGAAGATTGATATTCTTCACAAACAAG
  CTTCAGGAAATATTGTATATGGGGTATTTATGCTACATAAGAAAACAGTAAATCAGAAGA
  CCACAATCTACGAAATTCAGGATGATAGAGGAAAAATGGATGTAGTGGGGACAGGACAAT
  GTCACAATATCCCCTGTGAAGAAGGAGATAAGCTCCAACTTTTCTGCTTTCGACTTAGAA
  AAAAGAACCAGATGTCAAAACTGATTTCAGAAATGCATAGTTTTATCCAGATAAAGAAAA
  AAACAAACCCGAGAAACAATGACCCCAAGAGCATGAAGCTACCCCAGGAACAGAGTCAGC
  TTCCAAATCCTTCAGAGGCCAGCACAACCTTCCCTGAGAGCCATCTTCGGACTCCTCAGA
  TGCCACCAACAACTCCATCCAGCAGTTTCTTCACCAAGAAAAGTGAAGACACAATCTCCA
  AAATGAATGACTTCATGAGGATGCAGATACTGAAGGAAGGGAGTCATTTTCCAGGACCGT
  TCATGACCAGCATAGGCCCAGCTGAGAGCCATCCCCACACTCCTCAGATGCCTCCATCAA
  CACCAAGCAGCAGTTTCTTAACCACGTTGAAACCAAGACTGAAGACTGAACCTGAAGAAG
  TTTCCATAGAAGACAGTGCCCAGAGTGACCTCAAAGAAGTGATGGTGCTGAACGCAACAG
  AATCATTTGTATATGAGCCCAAAGAGCAGAAGAAAATGTTTCATGCCACAGTGGCAACTG
  AGAATGAAGTCTTCCGAGTGAAGGTTTTTAATATTGACCTAAAGGAGAAGTTCACCCCAA
  AGAAGATCATTGCCATAGCAAATTATGTTTGCCGCAATGGGTTCCTGGAGGTATATCCTT
  TCACACTTGTGGCTGATGTGAATGCTGACCGAAACATGGAGATCCCAAAAGGATTGATTA
  GAAGTGCCAGCGTAACTCCTAAAATCAATCAGCTTTGCTCACAAACTAAAGGAAGTTTTG
  TGAATGGGGTGTTTGAGGTACATAAGAAAAATGTAAGGGGTGAATTCACTTATTATGAAA
  TACAAGATAATACAGGGAAGATGGAAGTGGTGGTGCATGGACGACTGACCACAATCAACT
  GTGAGGAAGGAGATAAACTGAAACTCACCTGCTTTGAATTGGCACCGAAAAGTGGGAATA
  CCGGGGAGTTGAGATCTGTAATTCATAGTCACATCAAGGTCATCAAGACCAGGAAAAACA
  AGAAAGACATACTCAATCCTGATTCAAGTATGGAAACTTCACCAGACTTTTTCTTCTAAA
  ATCTGGATGTCATTGACGATAATGTTTATGGAGATAAGGTCTAAGTGCCTAAAAAAATGT
  ACATATACCTGGTTGAAATACAACACTATACATACACACCACCATATATACTAGCTGTTA
  ATCCTATGGAATGGGGTATTGGGAGTGCTTTTTTAATTTTTCATAGTTTTTTTTTAATAA
  AATGGCATATTTTGCATCTACAACTTCTATAATTTGAAAAAATAAATAAACATTATCTTT
  TTTGTGAAAAAAAAAAAAAAAAAACTCGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005531 unedited
  TTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGAATAGGAGCAAG
  CCAGCACTAGTCAGCTAACTAAGTGACTCAACCAAGGCCTTTTTTCCTTGTTATCTTTGC
  AGATACTTCATTTTCTTAGCGTTTCTGGAGATTACAACATCCTGCGGTTCCGTTTCTGGG
  AACTTTACTGATTTATCTCCCCCCTCACACAAATAAGCATTGATTCCTGCATTTCTGAAG
  ATCTCAAGATCTGGACTACTGTTGAAAAAATTTCCAGTGAGGCTCACTTATGTCTGTAAA
  GATGGGAAAAAAATACAAGAACATTGTTCTACTAAAAGGATTAGAGGTCATCAATGATTA
  TCATTTTAGAATGGTTAAGTCCTTACTGAGCAACGATTTAAAACTTAATTTAAAAATGAG
  AGAAGAGTATGACAAAATTCAGATTGCTGACTTGATGGAAGAAAAGTTCCGAGGTGATGC
  TGGTTTGGGCAAACTAATAAAAATTTTCGAAGATATACCAACGCTTGAAGACCTGGCTGA
  AACTCTTAAAAAAGAAAAGTTAAAAGTAAAAGGACCAGCCCTATCAAGAAAGAGGAAGAA
  GGAAGTGGATGCTACTTCACCTGCACCCTCCACAAGCAGCACTGTCAAAACTGAAGGAGC
  AGAGGCAACTCCTGGAGCTCAGAAAAGAAAAAAATCAACCANAGAAAAGGCTGCACCCAA
  AGGGAGTAANGGTGTCCGAGAACAGACTCACCCTCCTCTCNCTGCAGAGCCCGCATGTCC
  ACAGCCATGGGCCGTTNCCCATCTCCCAAGACCTCATTGTCAGCTTCACCCACACTTCTT
  TCACTGAGAACCCGAAACAGTGGCCAATGTCAGTTACTCCCCAAAGAAATGTCTCAAAAC
  CCCCATGATAGGGAAGTTCTGAT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005531 unedited
  CGCGGCCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTAACAAAAAAGAAATGTTTA
  TTTATTTTTTCAAATTATAGAAGTTGTAGATGCAAAATATGCCATTTTATTAAAAAAAAA
  CTATGAAAAATTAAAAAAGCACTCCCAATACCCCATTCCATAGGATTAACAGCTAGTATA
  TATGGTGGTGTGTATGTATAGTGTTGTATTTCAACCAGGTATATGTACATTTTTTTAGGC
  ACTTAAACCTTATCTCCATAAACATTATCGTCAATGACATCCAGATTTTAGAAGAAAAAG
  TCTGGTGAAGTTTCCATACTTGAATCAGGATTGAGTATGTCTTTCTTGTTTTTCCTGGAC
  TTGATGACCTTGATGCGACTATGAATTACAGATCTCAACCGCCCGGTATTGCCACATTTC
  GGTGCCCAACACAAACCTCGTTGACTCGCCGCTCGATCCCCTTCCTTCCCCTTCCACCGC
  TCCACCCTGTAATCCCGACCCCCCGATCATTCTCCCCGATCCACCATCGCGCCACCACCA
  CGCATCCTCACCCTTCATTGTATCTCCTTCATCTCCCCCCCGCCACTAACACGCCCCTCC
  CCCCCATAATCCCTCCCTCTCTCCCACTTCCTCATTCGCGTTTACTTTCCCCCCGCATCC
  TAACCCCGTCCTTATATCGCCCTCCCCAGTTGTCCTAATGCTACTCTCCGACCGCCCCCC
  CATCCCGCTATCGCCTCCCCCCCCCGCGCATCTTTACGTTCCTCTCATGCCACTCTCCCG
  CCTTTTACCGCCCTCCTACCTCCACCTACGTTTCTGTACTTATGTATATCCATCGCACTC
  ATACTGCCCGTTCGCCGTACTTCCCCCTTCTCTTATAAGCCCGCTCCAGTTTATNTATCC
  CCTCACTCCGTCTCCAATTTCCCCCATTTACCAATCCTCCATTACTACGATCGCACTCTA
  CACGTCGTATGTCTGTTTCCTCCGNATCCTACCCCTATCTTGTTTACGTCTCTAGTCCTC
  C
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005531
• Insert Size: 2770 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005531.1, NP_005522.1
• RefSeq Size: 2709 bp
• RefSeq ORF: 2190 bp
• Locus ID: 3428
• UniProt ID: Q16666
• Cytogenetics: 1q23.1
• Domains: PAAD_DAPIN, HIN
• Protein Families: Transcription Factors
• Gene Summary: This gene encodes a member of the HIN-200 (hematopoietic interferon-inducible nuclear antigens with 200 amino acid repeats) family of cytokines. The encoded protein contains domains involved in DNA binding, transcriptional regulation, and protein-protein interactions. The protein localizes to the nucleoplasm and nucleoli, and interacts with p53 and retinoblastoma-1. It modulates p53 function, and inhibits cell growth in the Ras/Raf signaling pathway. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2011]
  Transcript Variant: This variant (2) consists of 11 exons and encodes isoform 2 (also known as isoform B, PMIDs:9718316 and 12588705). Note the CDS was annotated to initiate translation from an in-frame downstream AUG, as it is flanked by a strong Kozak signal, and is better conserved than the upstream AUG located 4 codons upstream.