MRE11 (NM_005591) Human Untagged Clone

CAT#: SC116673

MRE11A (untagged)-Human MRE11 meiotic recombination 11 homolog A (S. cerevisiae) (MRE11A), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MRE11
• Synonyms: ATLD; HNGS1; MRE11A; MRE11B
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC116673 sequence for NM_005591 edited (data generated by NextGen Sequencing)
  ATGAGTACTGCAGATGCACTTGATGATGAAAACACATTTAAAATATTAGTTGCAACAGAT
  ATTCATCTTGGATTTATGGAGAAAGATGCAGTCAGAGGAAATGATACGTTTGTAACACTC
  GATGAAATTTTAAGACTTGCCCAGGAAAATGAAGTGGATTTTATTTTGTTAGGTGGTGAT
  CTTTTTCATGAAAATAAGCCCTCAAGGAAAACATTACATACCTGCCTCGAGTTATTAAGA
  AAATATTGTATGGGTGATCGGCCTGTCCAGTTTGAAATTCTCAGTGATCAGTCAGTCAAC
  TTTGGTTTTAGTAAGTTTCCATGGGTGAACTATCAAGATGGCAACCTCAACATTTCAATT
  CCAGTGTTTAGTATTCATGGCAATCATGACGATCCCACAGGGGCAGATGCACTTTGTGCC
  TTGGACATTTTAAGTTGTGCTGGATTTGTAAATCACTTTGGACGTTCAATGTCTGTGGAG
  AAGATAGACATTAGTCCGGTTTTGCTTCAAAAAGGAAGCACAAAGATTGCGCTATATGGT
  TTAGGATCCATTCCAGATGAAAGGCTCTATCGAATGTTTGTCAATAAAAAAGTAACAATG
  TTGAGACCAAAGGAAGATGAGAACTCTTGGTTTAACTTATTTGTGATTCATCAGAACAGG
  AGTAAACATGGAAGTACTAACTTCATTCCAGAACAATTTTTGGATGACTTCATTGATCTT
  GTTATCTGGGGCCATGAACATGAGTGTAAAATAGCTCCAACCAAAAATGAACAACAGCTG
  TTTTATATCTCACAACCTGGAAGCTCAGTGGTTACTTCTCTTTCCCCAGGAGAAGCTGTA
  AAGAAACATGTTGGTTTGCTGCGTATTAAAGGGAGGAAGATGAATATGCATAAAATTCCT
  CTTCACACAGTGCGGCAGTTTTTCATGGAGGATATTGTTCTAGCTAATCATCCAGACATT
  TTTAACCCAGATAATCCTAAAGTAACCCAAGCCATACAAAGCTTCTGTTTGGAGAAGATT
  GAAGAAATGCTTGAAAATGCTGAACGGGAACGTCTGGGTAATTCTCACCAGCCAGAGAAG
  CCTCTTGTACGACTGCGAGTGGACTATAGTGGAGGTTTTGAACCTTTCAGTGTTCTTCGC
  TTTAGCCAGAAATTTGTGGATCGGGTAGCTAATCCAAAAGACATTATCCATTTTTTCAGG
  CATAGAGAACAAAAGGAAAAAACAGGAGAAGAGATCAACTTTGGGAAACTTATCACAAAG
  CCTTCAGAAGGAACAACTTTAAGGGTAGAAGATCTTGTAAAACAGTACTTTCAAACCGCA
  GAGAAGAATGTGCAGCTCTCACTGCTAACAGAAAGAGGGATGGGTGAAGCAGTACAAGAA
  TTTGTGGACAAGGAGGAGAAAGATGCCATTGAGGAATTAGTGAAATACCAGTTGGAAAAA
  ACACAGCGATTTCTTAAAGAACGTCATATTGATGCCCTCGAAGACAAAATCGATGAGGAG
  GTACGTCGTTTCAGAGAAACCAGACAAAAAAATACTAATGAAGAAGATGATGAAGTCCGT
  GAGGCTATGACCAGGGCCAGAGCACTCAGATCTCAGTCAGAGGAGTCTGCTTCTGCCTTT
  AGTGCTGATGACCTTATGAGTATAGATTTAGCAGAACAGATGGCTAATGACTCTGATGAT
  AGCATCTCAGCAGCAACCAACAAAGGAAGAGGCCGAGGAAGAGGTCGAAGAGGTGGAAGA
  GGGCAGAATTCAGCATCGAGAGGAGGGTCTCAAAGAGGAAGAGCAGACACTGGTCTGGAG
  ACTTCTACCCGTAGCAGGAACTCAAAGACTGCTGTGTCAGCATCTAGAAATATGTCTATT
  ATAGATGCCTTTAAATCTACAAGACAGCAGCCTTCCCGAAATGTCACTACTAAGAATTAT
  TCAGAGGTGATTGAGGTAGATGAATCAGATGTGGAAGAAGACATTTTTCCTACCACTTCA
  AAGACAGATCAAAGGTGGTCCAGCACATCACCCAGCAAAATCATGTCCCAGAGTCAAGTA
  TCGAAAGGGGTTGATTTTGAATCAAGTGAGGATGATGATGATGATCCTTTTATGAACACT
  AGTTCTTTAAGAAGAAATAGAAGATAA
  
  Clone variation with respect to NM_005591.3
  2011 t=>c
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005591 unedited
  GCACGAGGGCGGGTTCTCAGAGGCAAGTTCAGACCGTGTTGTTTTCTTTTCACGGATCCT
  GCCCTTTCTTCCCGAAAAGAAGACAGCCTTGGGTCGCGATTGTGGGGCTTCGAAGAGTCC
  AGCAGTGGGAATTTCTAGAATTTGGAATCGAGTGCATTTTCTGACATTTGAGTACAGTAC
  CCAGGGGTTCTTGGAGAAGAACCTGGTCCCAGAGGAGCTTGACTGACCATAAAAATGAGT
  ACTGCAGATGCACTTGATGATGAAAACACATTTAAAATATTAGTTGCAACAGATATTCAT
  CTTGGATTTATGGAGAAAGATGCAGTCAGAGGAAATGATACGTTTGTAACACTCGATGAA
  ATTTTAAGACTTGCCCAGGAAAATGAAGTGGATTTTATTTTGTTAGGTGGTGATCTTTTT
  CATGAAAATAAGCCCTCAGGAAAACATTACATACCTGCCTCGAGTTATTAAGAAAATATT
  GTATGGGTGATCGGCCTGTCCAGTTTGAAATTCTCAGTGATCAGTCAGTCAACTTTGGTT
  TTAGTAAGTTTCCATGGGTGAACTATCAAGATGGCAACCTCAACA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005591 unedited
  GAACGCGGCACGCAATCTATAATCGAGTTTTTTTTTTTTTTTTTTCTTATGAAAAGAAAG
  TAAAAGGTAGCTTTATTTTAATCTTTACTCAAGAGTGATATTGAAACAAAGCTCTTACTA
  CAACAACCAGGTTAAAAAAACAAGGTGAATCAATGCTATTGTATGTCTGTGAACTAAAAA
  TTTCTTACTTATGGAGTTATGCTCAGGAAACAATACTTAAAATCTTAAACTGTAAACTCT
  TAGCTTGTAACATTTTCATTTTTCCTGTATCTTGCATGTTTCTCAGTGCCATTAAATATA
  TTATCTTCTATTTCTTCTTAAAGAACTAGTGTTCATAAAAGGATCATCATCATCATCCTC
  ACTTGATTCAAAATCAACCCCTTTCGATACTTGACTCTGGGACATGATTTTGCTGGGTGA
  TGTGCTGGACCACCTTTGATCTGTCTTTGAAGTGGTAGGAAAAATGTCTTCTTCCACATC
  TGATTCATCTACCTCAATCACCTCTGAATAATTCTTAGTAGTGACATTTCGGGAAGGCTG
  CTGTCTTGTAGATTTAAAGGCATCTATAATAGACATATTTCTAGATGCTGACACAGCAGT
  CTTTGAGTTCCTGCTACGGGTAGAAGTCTCCAGACCAGTGTCTGCTCTTCCTCTTTGAGA
  CCCTCCTCTCGATGCTGAATTCTGCCCTCTTCCACCTCTTCGACCTCTTCCTCGGCCTCT
  TCCTTTGTTGGTTGCTGCTGAGATGCTATCATCAGAGTCATTAGCCATCTGTTCTGCTAA
  ATCTATACTCATAAGGTCATCAGCACTTAAAGCAGAAACAGACTTCTCTGACTGAGATCT
  GAGTGCTCTGGCCCTGGTCATAGCCTCACAGACTCATCATCTCTTCATTAAAATTTTTTG
  CCTGGTTCTCTGAACAAGTACCTCTCATG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005591
• Insert Size: 3040 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005591.3, NP_005582.1
• RefSeq Size: 5141 bp
• RefSeq ORF: 2127 bp
• Locus ID: 4361
• UniProt ID: P49959
• Cytogenetics: 11q21
• Domains: Metallophos, Mre11_DNA_bind
• Protein Families: Druggable Genome, Stem cell - Pluripotency
• Protein Pathways: Homologous recombination, Non-homologous end-joining
• Gene Summary: This gene encodes a nuclear protein involved in homologous recombination, telomere length maintenance, and DNA double-strand break repair. By itself, the protein has 3' to 5' exonuclease activity and endonuclease activity. The protein forms a complex with the RAD50 homolog; this complex is required for nonhomologous joining of DNA ends and possesses increased single-stranded DNA endonuclease and 3' to 5' exonuclease activities. In conjunction with a DNA ligase, this protein promotes the joining of noncomplementary ends in vitro using short homologies near the ends of the DNA fragments. This gene has a pseudogene on chromosome 3. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) encodes the longer isoform of this protein.