ALDH6A1 (NM_005589) Human Untagged Clone

CAT#: SC116671

ALDH6A1 (untagged)-Human aldehyde dehydrogenase 6 family, member A1 (ALDH6A1), nuclear gene encoding mitochondrial protein

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ALDH6A1
• Synonyms: MMSADHA; MMSDH
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_005589, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCGGCGCTATTGGCGGCGGCGGCAGTGCGAGCCCGGATCCTGCAGGTTTCTTCCAAGGTGAAATCCA
  GTCCCACCTGGTATTCAGCATCTTCCTTCTCTTCTTCAGTGCCAACTGTAAAGCTCTTCATTGGTGGGAA
  ATTCGTTGAATCCAAAAGTGACAAATGGATCGATATCCACAACCCAGCCACCAATGAGGTCATTGGTCGG
  GTCCCTCAGGCCACCAAGGCAGAAATGGATGCAGCCATTGCTTCCTGCAAACGTGCTTTTCCTGCATGGG
  CAGACACTTCAGTATTAAGCCGCCAGCAGGTCTTGCTCCGCTATCAACAACTTATTAAAGAAAACTTGAA
  AGAAATTGCCAAGTTAATCACATTGGAACAAGGGAAGACCCTAGCTGATGCTGAAGGAGATGTATTTCGA
  GGCCTTCAGGTGGTTGAGCATGCCTGTAGTGTGACATCCCTCATGATGGGAGAGACCATGCCATCCATCA
  CCAAAGACATGGACCTTTATTCCTACCGTCTGCCTCTGGGAGTGTGTGCAGGCATTGCTCCATTCAATTT
  TCCTGCCATGATCCCCCTTTGGATGTTTCCCATGGCCATGGTGTGTGGAAATACCTTCCTAATGAAACCA
  TCTGAGCGAGTCCCTGGAGCAACTATGCTTCTTGCTAAGTTGCTCCAGGATTCTGGTGCCCCTGATGGAA
  CATTAAACATCATCCATGGACAGCATGAAGCTGTAAATTTTATTTGCGATCATCCGGACATCAAAGCAAT
  CAGCTTTGTGGGATCCAACAAGGCAGGAGAGTATATCTTCGAGAGAGGATCAAGACATGGCAAGAGGGTT
  CAAGCCAATATGGGAGCCAAGAACCATGGGGTAGTCATGCCAGATGCCAATAAGGAAAATACCCTGAACC
  AGCTGGTTGGGGCAGCATTTGGAGCTGCTGGTCAGCGCTGCATGGCTCTTTCAACAGCAGTCCTTGTGGG
  AGAAGCCAAGAAGTGGCTGCCAGAGCTGGTGGAGCATGCCAAAAACCTGAGAGTCAATGCAGGAGATCAG
  CCTGGAGCTGATCTTGGCCCTCTGATCACTCCCCAGGCCAAAGAGCGAGTCTGTAATCTGATTGATAGTG
  GAACAAAGGAGGGAGCTTCCATCCTTCTTGATGGACGAAAAATTAAAGTGAAAGGCTATGAAAATGGCAA
  CTTTGTTGGACCAACCATCATCTCGAATGTCAAGCCAAATATGACCTGTTACAAAGAGGAGATTTTTGGT
  CCAGTTCTTGTGGTTCTGGAGACAGAAACATTGGATGAAGCCATCCAGATTGTAAATAACAACCCATATG
  GAAATGGAACTGCCATCTTCACCACCAATGGAGCCACTGCTCGGAAATATGCCCACTTGGTGGATGTTGG
  ACAGGTGGGAGTGAATGTCCCCATTCCAGTGCCTTTGCCAATGTTCTCATTCACCGGCTCTCGATCCTCC
  TTCAGGGGAGACACCAATTTCTATGGCAAACAGGGCATCCAATTCTACACTCAGTTAAAGACCATTACTT
  CTCAGTGGAAAGAAGAAGATGCTACTCTTTCCTCACCTGCTGTTGTCATGCCTACCATGGGCCGTTAG
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005589 unedited
  AGGATTTTGTAATACGACTCACTATAGGGCGGCCGCGACTTCGGCACGAGGCCTCGTGCC
  GAATTCGGCACGAGGCTAGGGCGGCCGAGAGCCATGGCGGCGCTATTGGCGGCGGCGGCA
  GTGCGAGCCCGGATCCTGCAGGTTTCTTCCAAGGTGAAATCCAGTCCCACCTGGTATTCA
  GCATCTTCCTTCTCTTCTTCAGTGCCAACTGTAAAGCTCTTCATTGGTGGGAAATTCGTT
  GAATCCAAAAGTGACAAATGGATCGATATCCACAACCCAGCCACCAATGAGGTCATTGGT
  CGGGTCCCTCAGGCCACCAAGGCAGAAATGGATGCAGCCATTGCTTCCTGCAAACGTGCT
  TTTCCTGCATGGGCAGACACTTCAGTATTAAGCCGCCAGCAGGTCTTGCTCCGCTATCAA
  CAACTTATTAAAGAAAACTTGAAAGAAATTGCCAAGTTAATCACATTGGAACAAGGGAAG
  ACCCTAGCTGATGCTGAAGGAGATGTATTTCGAGGCCTTCAGGTGGTTGAGCATGCCTGT
  AGTGTGACATCCCTCATGATGGGAGAGACCATGCCATCCATCACCAAAGACATGGACCTT
  TATTCCTACCGTCTGCCTCTGGGAGTGTGTGCAGGCATTGCTCCATTCAATTTTCCTGCC
  ATGATCCCCCTTTGGATGTTTCCCATGGCCATGGTGTGTGGAAATACCTTCCTAATGAAA
  CCATCTGAGCGAGTCCCTGGAGCAACTATGCTTCTTGCTAAGTTGCTCCACGATTCTGGT
  GCCCCTGATGGAACATTANACATCATCCATGGACAGCATGAAGCTGTAAATTTTATTTTG
  CGATCATCCGGACATCAAAGCAATCAGCTTTGTTGGGATCCAACAGGCAGGAGAGTATAT
  CTTCGACAGAGATCAAGACATGCAGGAGGGTCAAGCCATATGGGAGCCAGACCATGGGGT
  AA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005589 unedited
  CGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTCTCTTTAAAAGTTTTTAATTT
  ATAATTTGTTATTCATAATTAGTACAATGTAGAATCTGAGAAATTTCAAATGAAGCCACA
  TTAGAACAAAAAGAAAATACTGACTTTTTAGCCGCGATCACATGAATATAACTGATGAGT
  TTTTTTCATCTAAGAAATAAGAAACTCTGAGCAGCAAATAGTTGGCCAGATGAGTTGTTT
  CTAATTAGAGCATCACAGTTCTGATTAGTGTGTTTTTTTAGAAGTGATTTCCCTTAAATA
  TAAGGAAAATGTTAGGAATCTCTGTGACTACATTTAATTCCACTTGGAATATAGACAATA
  ATGACCTTTTATTTAACCCAGCCTATAGTTGTTGGATTGGATTAAATATCACTACCAGGG
  CCAGGTGCGGTGGTTCATTTTCTTTACATACACTGGCTTTTCTCCCCTTCAAATCATCAG
  AAAATGGGATAATTTTTAGAAATCAGGTTTAAGCACTACTTTCAGATAAGCATTTCATAG
  TTACAACAGTTACCTCAAAATAGAAGTATGAAGAGCAAGTGAGAAATCTGGTTTCATTGT
  TACACTAGGCAGTTCCCTATAGAAACTTTGCAGGGGTTAATAGTCCTGNAGAAGATTTTA
  GTTACAATGTATTCCAATCCCATCGATCTGATCTGAGCAAAGCTGACAAATGAAGCTGGT
  CAAAAATAAGGGAGATTACTCAGGATGGAGTCAGTCTTAAACAACCTTGTTCTAACGGCC
  ATGGTAGGCATGACAACAGCAGGTGAGGGAAGAGTAGCATCTTCTTCTTNCACTGAGAAG
  TAATGGTCTTCACTGAGTTANAATTGGAGCCCTGTTTGCCTANAAATGTGTCTCCCTGAA
  GAGATCGGAGCCGTGATGAAAACTTGCAAGGCCTGGATGGGACATTACTCACTGTCACAT
  CCCAGGGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005589
• Insert Size: 2500 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005589.2, NP_005580.1
• RefSeq Size: 2183 bp
• RefSeq ORF: 1608 bp
• Locus ID: 4329
• UniProt ID: Q02252
• Cytogenetics: 14q24.3
• Domains: aldedh
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Inositol phosphate metabolism, Metabolic pathways, Propanoate metabolism, Valine, leucine and isoleucine degradation
• Gene Summary: This gene encodes a member of the aldehyde dehydrogenase protein family. The encoded protein is a mitochondrial methylmalonate semialdehyde dehydrogenase that plays a role in the valine and pyrimidine catabolic pathways. This protein catalyzes the irreversible oxidative decarboxylation of malonate and methylmalonate semialdehydes to acetyl- and propionyl-CoA. Methylmalonate semialdehyde dehydrogenase deficiency is characterized by elevated beta-alanine, 3-hydroxypropionic acid, and both isomers of 3-amino and 3-hydroxyisobutyric acids in urine organic acids. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013]
  Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.