NGAL (LCN2) (NM_005564) Human Untagged Clone

CAT#: SC116655

LCN2 (untagged)-Human lipocalin 2 (LCN2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NGAL
• Synonyms: 24p3; MSFI; NGAL; p25
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC116655 sequence for NM_005564 edited (data generated by NextGen Sequencing)
  ATGCCCCTAGGTCTCCTGTGGCTGGGCCTAGCCCTGTTGGGGGCTCTGCATGCCCAGGCC
  CAGGACTCCACCTCAGACCTGATCCCAGCCCCACCTCTGAGCAAGGTCCCTCTGCAGCAG
  AACTTCCAGGACAACCAATTCCAGGGGAAGTGGTATGTGGTAGGCCTGGCAGGGAATGCA
  ATTCTCAGAGAAGACAAAGACCCGCAAAAGATGTATGCCACCATCTATGAGCTGAAAGAA
  GACAAGAGCTACAATGTCACCTCCGTCCTGTTTAGGAAAAAGAAGTGTGACTACTGGATC
  AGGACTTTTGTTCCAGGTTGCCAGCCCGGCGAGTTCACGCTGGGCAACATTAAGAGTTAC
  CCTGGATTAACGAGTTACCTCGTCCGAGTGGTGAGCACCAACTACAACCAGCATGCTATG
  GTGTTCTTCAAGAAAGTTTCTCAAAACAGGGAGTACTTCAAGATCACCCTCTACGGGAGA
  ACCAAGGAGCTGACTTCGGAACTAAAGGAGAACTTCATCCGCTTCTCCAAATCTCTGGGC
  CTCCCTGAAAACCACATCGTCTTCCCTGTCCCAATCGACCAGTGTATCGACGGCTGA
  
  Clone variation with respect to NM_005564.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005564 unedited
  NGGGTCAGAATTTGTATACGACTCCTATAGGGCGGCCGCGAATCGGCACCAGCTCTTCCA
  CCCCTGCCAGGCCCAGCAGCCACCACAGCGCCTGCTTCCTCGGCCCTGAAATCATGCCCC
  TAGGTCTCCTGTGGCTGGGCCTAGCCCTGTTGGGGGCTCTGCATGCCCAGGCCCAGGACT
  CCACCTCAGACCTGATCCCAGCCCCACCTCTGAGCAAGGTCCCTCTGCAGCAGAACTTCC
  AGGACAACCAATTCCAGGGGAAGTGGTATGTGGTAGGCCTGGCAGGGAATGCAATTCTCA
  GAGAAGACAAAGACCCGCAAAAGATGTATGCCACCATCTATGAGCTGAAAGAAGACAAGA
  GCTACAATGTCACCTCCGTCCTGTTTAGGAAAAAGAAGTGTGACTACTGGATCAGGACTT
  TTGTTCCAGGTTGCCAGCCCGGCGAGTTCACGCTGGGCAACATTAAGAGTTACCCTGGAT
  TAACGAGTTACCTCGTCCGAGTGGTGAGCACCAACTACAACCAGCATGCTATGGTGTTCT
  TCAAGAAAGTTTCTCAAAACAGGGAGTACTTCAAGATCACCCTCTACGGGAGAACCAAGG
  AGCTGACTTCGGAACTAAAGGAGAACTTCATCCGCTTCTCCAAATCTCTGGGCCTCCCTG
  AAAACCACATCGTCTTCCCTGTCCCAATCGACCAGTGTATCGACGGCTGAGTGCACAGGT
  GCCGCCAGCTGCCGCACCAGCCCGAACACCATTGAGGGAGCTGGGAGACCCTCCCCCAGT
  GCCACCATGCAGCTGCTCCCCAGCCACCCCGCTGATGGAGCCCCACCTTGTCTGCTNAAT
  AACATGTGCCCTCAGAANAAAAAAAAAAAAACTCGACTCTAGATTGCGCCGCCGTCATAG
  CTGTTTCTGACAGATCCGGNTGCATCCTGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005564 unedited
  CTATGACACGCGGCACGCAATCTAGAGATCGAGTTTTTTTTTTTTTTTTTTCTGAGGGCA
  CATGTTTATTTAGCAGACAAGGTGGGGCTCCATCAGCGGGGTGGCCTGGGGAGCAGCTGC
  ATGGGTGGCACTGTGGGGAGGGTCTCCCAGCTCCCTCAATGGTGTTCGGGCTGGTGCGGC
  AGCTGGCGGCACCTGTGCACTCAGCCGTCGATACACTGGTCGATTGGGACAGGGAAGACG
  ATGTGGTTTTCAGGGAGGCCCAGAGATTTGGAGAAGCGGATGAAGTTCTCCTTTAGTTCC
  GAAGTCAGCTCCTTGGTTCTCCCGTAGAGGGTGATCTTGAAGTACTCCCTGTTTTGAGAA
  ACTTTCTTGAAGAACACCATAGCATGCTGGTTGTAGTTGGTGCTCACCACTCGGACGAGG
  TAACTCGTTAATCCAGGGTAACTCTTAATGTTGCCCAGCGTGAACTCGCCGGGCTGGCAA
  CCTGNAACAAAAGTCCTGATCCAGTANTCACACTTTTTTTTTCTAAACAGGACGGAGGTG
  ACATTGAAGCTCTTGTCTTCTTTCAACCCATANATGGTGGCATACATCTTTTGCGGGTCT
  TTGTCTCTTTGAGAATTGAATTTCCTTGCAGCCTACCACATTCCACTTCCCCTGAAATTG
  GTTGTCCTGGTAATTTTTGCTGCAAAAGGCCTTTGTCTCAAAGAGGGTCTAGGTAACCAC
  CCCAAGGTAGAACCTTTGGTTTTGGTATGCTAAATATTCAATAGTTTTTTTCTTTTTTTC
  TCGACCACTCAGCACTTTGATCTAANNNNCCCGCCAACTTCCTCATTTCNAGATTTTTTC
  TCCATTTTATCCCTTCTATCTTGCGCCACCTTGTCCTTCTCGTTATCTGCTACGCCCCTC
  CCTTTTTTTTTTTTTATTCTCCCCCTCCTTTTTTTATAACACAGCACACTCATATCTATC
  CCCATACCTCCATCA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005564
• Insert Size: 850 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005564.2, NP_005555.2
• RefSeq Size: 845 bp
• RefSeq ORF: 597 bp
• Locus ID: 3934
• UniProt ID: P80188
• Cytogenetics: 9q34.11
• Domains: lipocalin
• Protein Families: Secreted Protein
• Gene Summary: This gene encodes a protein that belongs to the lipocalin family. Members of this family transport small hydrophobic molecules such as lipids, steroid hormones and retinoids. The protein encoded by this gene is a neutrophil gelatinase-associated lipocalin and plays a role in innate immunity by limiting bacterial growth as a result of sequestering iron-containing siderophores. The presence of this protein in blood and urine is an early biomarker of acute kidney injury. This protein is thought to be be involved in multiple cellular processes, including maintenance of skin homeostasis, and suppression of invasiveness and metastasis. Mice lacking this gene are more susceptible to bacterial infection than wild type mice. [provided by RefSeq, Sep 2015]