SGK1 (NM_005627) Human Untagged Clone

CAT#: SC116597

SGK1 (untagged)-Human serum/glucocorticoid regulated kinase 1 (SGK1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SGK1
• Synonyms: SGK
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_005627 edited
  ATGACGGTGAAAACTGAGGCTGCTAAGGGCACCCTCACTTACTCCAGGATGAGGGGCATG
  GTGGCAATTCTCATCGCTTTCATGAAGCAGAGGAGGATGGGTCTGAACGACTTTATTCAG
  AAGATTGCCAATAACTCCTATGCATGCAAACACCCTGAAGTTCAGTCCATCTTGAAGATC
  TCCCAACCTCAGGAGCCTGAGCTTATGAATGCCAACCCTTCTCCTCCACCAAGTCCTTCT
  CAGCAAATCAACCTTGGCCCGTCGTCCAATCCTCATGCTAAACCATCTGACTTTCACTTC
  TTGAAAGTGATCGGAAAGGGCAGTTTTGGAAAGGTTCTTCTAGCAAGACACAAGGCAGAA
  GAAGTGTTCTATGCAGTCAAAGTTTTACAGAAGAAAGCAATCCTGAAAAAGAAAGAGGAG
  AAGCATATTATGTCGGAGCGGAATGTTCTGTTGAAGAATGTGAAGCACCCTTTCCTGGTG
  GGCCTTCACTTCTCTTTCCAGACTGCTGACAAATTGTACTTTGTCCTAGACTACATTAAT
  GGTGGAGAGTTGTTCTACCATCTCCAGAGGGAACGCTGCTTCCTGGAACCACGGGCTCGT
  TTCTATGCTGCTGAAATAGCCAGTGCCTTGGGCTACCTGCATTCACTGAACATCGTTTAT
  AGAGACTTAAAACCAGAGAATATTTTGCTAGATTCACAGGGACACATTGTCCTTACTGAC
  TTCGGACTCTGCAAGGAGAACATTGAACACAACAGCACAACATCCACCTTCTGTGGCACG
  CCGGAGTATCTCGCACCTGAGGTGCTTCATAAGCAGCCTTATGACAGGACTGTGGACTGG
  TGGTGCCTGGGAGCTGTCTTGTATGAGATGCTGTATGGCCTGCCGCCTTTTTATAGCCGA
  AACACAGCTGAAATGTACGACAACATTCTGAACAAGCCTCTCCAGCTGAAACCAAATATT
  ACAAATTCCGCAAGACACCTCCTGGAGGGCCTCCTGCAGAAGGACAGGACAAAGCGGCTC
  GGGGCCAAGGATGACTTCATGGAGATTAAGAGTCATGTCTTCTTCTCCTTAATTAACTGG
  GATGATCTCATTAATAAGAAGATTACTCCCCCTTTTAACCCAAATGTGAGTGGGCCCAAC
  GACCTACGGCACTTTGACCCCGAGTTTACCGAAGAGCCTGTCCCCAACTCCATTGGCAAG
  TCCCCTGACAGCGTCCTCGTCACAGCCAGCGTCAAGGAAGCTGCCGAGGCTTTCCTAGGC
  TTTTCCTATGCGCCTCCCACGGACTCTTTCCTCTGA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005627 unedited
  ATCTTGGATTTGTAACCGACTTCTATAGGCGGCACGCGAATTCGCACGAGGCCGAGCCGG
  TCTTTGAGCGCTAACGTCTTTCTGTCTCCCCGCGGTGGTGATGACGGTGAAAACTGAGGC
  TGCTAAGGGCACCCTCACTTACTCCAGGATGAGGGGCATGGTGGCAATTCTCATCGCTTT
  CATGAAGCAGAGGAGGATGGGTCTGAACGACTTTATTCAGAAGATTGCCAATAACTCCTA
  TGCATGCAAACACCCTGAAGTTCAGTCCATCTTGAAGATCTCCCAACCTCAGGAGCCTGA
  GCTTATGAATGCCAACCCTTCTCCTCCACCAAGTCCTTCTCAGCAAATCAACCTTGGCCC
  GTCGTCCAATCCTCATGCTAAACCATCTGACTTTCACTTCTTGAAAGTGATCGGAAAGGG
  CAGTTTTGGAAAGGTTCTTCTAGCAAGACACAAGGCAGAAGAAGTGTTCTATGCAGTCAA
  AGTTTTACAGAAGAAAGCAATCCTGAAAAAGAAAGAGGAGAAGCATATTATGTCGGAGCG
  GAATGTTCTGTTGAAGAATGTGAAGCACCCTTTCCTGGTGGGCCTTCACTTCTCTTTCCA
  GACTGCTGACAAATTGTACTTTGTCCTAGACTACATTAATGGTGGAGAGTTGTTCTACCA
  TCTCCAGAGGGAACGCTGCTTCCTGGAACCACGGGCTCGTTTCTATGCTGCTGANATAGC
  CAGTGCCTTGAGCTACCTGCATTCACTGAACATCGTTTATAGAGACTTANACCAGAGAAT
  ATNTTGCTAGATTACAGGGACACATTGTCCCTACTGACTTNCGACTCTGCAAGGAGAACA
  TTTGACACAACAGCACAACATCCACCTTCTGTGGCACGCCGGAGTATCTCGCACCTGAAG
  TGCT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005627 unedited
  TTCGCTTTGTACCGCGGCCCGCAATCTAGCATCGAGTTTTTTTTTTTTTTTTTTAAATAT
  TTTTCAAGGTTTTATTGCAAACCAAAATCAGTTTATCACACACAAAAAAAGTTGTGTGAT
  GGGATGAGGGAAGGATTGTACGTATTATAACCATGTTAATTACAGTACATTAAAATGGTG
  GTTTACATTACAAATAAGCCTGTAAGTTTAAATATACTAGTGTTATAACCCAATGTACAG
  ACGTTCTTTATACAATACATACAATTATCAGGAATGCAAAAAAAAAAACATAAATAATGC
  CCATTTTACAGGTGACATTTTAAACAATGAAAAACACCAACGGCTCTGACTGACAACTGG
  GGCATTGGTCCATAAAAACCCTTTCTAAAAATAGAAATATTTGTAGCAGTAATGCTTTCT
  TTAAGCATCTGAATACGAGTCATTGCAAGACCATTTCAATAAATTTTTAGTTATTAACCG
  TATCTTACAAAAAAGTCTACACATAAATTTATCTTCCAAATATGGAAGAAACAAACAATG
  TCCCACGTTGTAGTGTCCTGCAAGTGTAACATTGATGCTTATAACAAAATCCATCTGTGA
  TCAGGCATACCACACTCACACGACGGTTCACACAGCATATTCACAAGGGAAAATAAAGAA
  CTGAAACACTGCGATAGGAAAAGTTTTGGAGCTAACACATTCTCTTCAGACAATGCACAT
  TTCATATTATTTGTAATTAAAAGCCAAGACAGATCTGCCAGAAGACCTTTTTAGAACAGC
  GTCCCGCTTTCTAACAAAATTCCTGGCTTCCGTTTAAGGCCGCACTCCTACCGCTTGCTT
  TAGAGATAGCCCCCAGTTGTGAAGGAAGATTAAAATGAACACTTNTTGAGCTCATTGAGG
  AGATGCGCTTTCCAAAACTTCCAGCGACAGGTGGAATAAAAATGCTAACTTTCAAN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005627
• Insert Size: 2560 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005627.2, NP_005618.2
• RefSeq Size: 2386 bp
• RefSeq ORF: 1296 bp
• Locus ID: 6446
• UniProt ID: O00141
• Cytogenetics: 6q23.2
• Domains: pkinase, S_TK_X, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Gene Summary: This gene encodes a serine/threonine protein kinase that plays an important role in cellular stress response. This kinase activates certain potassium, sodium, and chloride channels, suggesting an involvement in the regulation of processes such as cell survival, neuronal excitability, and renal sodium excretion. High levels of expression of this gene may contribute to conditions such as hypertension and diabetic nephropathy. Several alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Jan 2009]
  Transcript Variant: This variant (1) represents the predominant transcript and encodes the shortest isoform (1).