EBI3 (NM_005755) Human Untagged Clone

CAT#: SC116552

EBI3 (untagged)-Human Epstein-Barr virus induced 3 (EBI3)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: EBI3
• Synonyms: IL-27B; IL27B; IL35B
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC116552 sequence for NM_005755 edited (data generated by NextGen Sequencing)
  ATGACCCCGCAGCTTCTCCTGGCCCTTGTCCTCTGGGCCAGCTGCCCGCCCTGCAGTGGA
  AGGAAAGGGCCCCCAGCAGCTCTGACACTGCCCCGGGTGCAATGCCGAGCCTCTCGGTAC
  CCGATCGCCGTGGATTGCTCCTGGACCCTGCCGCCTGCTCCAAACTCCACCAGCCCCGTG
  TCCTTCATTGCCACGTACAGGCTCGGCATGGCTGCCCGGGGCCACAGCTGGCCCTGCCTG
  CAGCAGACGCCAACGTCCACCAGCTGCACCATCACGGATGTCCAGCTGTTCTCCATGGCT
  CCCTACGTGCTCAATGTCACCGCCGTCCACCCCTGGGGCTCCAGCAGCAGCTTCGTGCCT
  TTCATAACAGAGCACATCATCAAGCCCGACCCTCCAGAAGGCGTGCGCCTAAGCCCCCTC
  GCTGAGCGCCAGCTACAGGTGCAGTGGGAGCCTCCCGGGTCCTGGCCCTTCCCAGAGATC
  TTCTCACTGAAGTACTGGATCCGTTACAAGCGTCAGGGAGCTGCGCGCTTCCACCGGGTG
  GGGCCCATTGAAGCCACGTCCTTCATCCTCAGGGCTGTGCGGCCCCGAGCCAGGTACTAC
  GTCCAAGTGGCGGCTCAGGACCTCACAGACTACGGGGAACTGAGTGACTGGAGTCTCCCC
  GCCACTGCCACAATGAGCCTGGGCAAGTAG
  
  Clone variation with respect to NM_005755.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005755 unedited
  NNNGGTTCAAATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACCAGGAGCAGA
  GCTGGCCGCAGCCATGACCCCGCAGCTTCTCCTGGCCCTTGTCCTCTGGGCCAGCTGCCC
  GCCCTGCAGTGGAAGGAAAGGGCCCCCAGCAGCTCTGACACTGCCCCGGGTGCAATGCCG
  AGCCTCTCGGTACCCGATCGCCGTGGATTGCTCCTGGACCCTGCCGCCTGCTCCAAACTC
  CACCAGCCCCGTGTCCTTCATTGCCACGTACAGGCTCGGCATGGCTGCCCGGGGCCACAG
  CTGGCCCTGCCTGCAGCAGACGCCAACGTCCACCAGCTGCACCATCACGGATGTCCAGCT
  GTTCTCCATGGCTCCCTACGTGCTCAATGTCACCGCCGTCCACCCCTGGGGCTCCAGCAG
  CAGCTTCGTGCCTTTCATAACAGAGCACATCATCAAGCCCGACCCTCCAGAAGGCGTGCG
  CCTAAGCCCCCTCGCTGAGCGCCAGCTACAGGTGCAGTGGGAGCCTCCCGGGTCCTGGCC
  CTTCCCAGAGATCTTCTCACTGAAGTACTGGATCCGTTACAAGCGTCAGGGAGCTGCGCG
  CTTCCACCGGGTGGGGCCCATTGAAGCCACGTCCTTCATCCTCAGGGCTGTGCGGCCCCG
  AGCCAGGTACTACGTCCAAGTGGCGGCTCAGGACCTCACAGACTACGGNGAACTGAGTGA
  CTGGAGTCTCCCCGCCACTGCCACAATGAGCCTGNGCAAGTAGCAAGGGCTTCCCGCTGC
  CTNCAGACGCACCTGNGTCCTCGCCACCCTAAGCCCCGGACACCTGTTGGAGGGCGGATG
  GGATCTGCCTAGCCTGGGCTGGAGTCCTTTGCTTGCTGCTGCTGAGCTGCCGGGCACCTC
  AGAGACCGACTTTTCCTNTGAGCCTCAGTTCTCTAGCTGAAAC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005755
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005755.2, NP_005746.2
• RefSeq Size: 1149 bp
• RefSeq ORF: 690 bp
• Locus ID: 10148
• UniProt ID: Q14213
• Cytogenetics: 19p13.3
• Domains: FN3
• Protein Families: Druggable Genome, Secreted Protein
• Gene Summary: This gene was identified by its induced expression in B lymphocytes in response Epstein-Barr virus infection. It encodes a secreted glycoprotein belonging to the hematopoietin receptor family, and heterodimerizes with a 28 kDa protein to form interleukin 27 (IL-27). IL-27 regulates T cell and inflammatory responses, in part by activating the Jak/STAT pathway of CD4+ T cells. [provided by RefSeq, Sep 2008]